• 한국전산유체공학회:학술대회논문집
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• 2004.10a
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• pp.15-18
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• 2004

A numerical method for simulating two-phase flows including surface force is presented. The method is based on fractional step method of finite volume formulation and the interface is tracked with PLIC VOF method. In the CSF model, as color function, f, representing the location of interface varies steeply in the interface region, we need to use smoothed function f to get accurate unit normal and the curvature. Peskin kernel is used to get smoothed function f. A spherical drop in static equilibrium and three-dimensional merging of gas bubble are tested, resulting in the validation of this method

• IMMUNE NETWORK
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• v.6 no.1
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• pp.13-19
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• 2006

Background: Granulocyte colony stimulating factor (G-CSF) is known as a cytokine central to the hematopoiesis of blood cells and to modulate their cellular functions. Besides granulocytes and their precursors, monocytes/macrophages and endothelial cells are direct target cells of G-CSF action. G-CSF influences immune cells in an anti inflammatory way. Methods: To evaluate whether G-CSF has a potential for preventing or ameliorating diseases characterized by mucosal inflammation, we used a mouse model with trinitrobenzene sulfonic acid (TNBS)-induced inflammatory colitis. To the mice model G-CSF was administrated daily by intraperitoneal injection. Macroscopic evaluation and immunohistochemical analysis of colonic tissues were performed. Results: Re combinant human G-CSF significantly inhibited LPS-induced TNF-${\alpha}$ mRNA expression in THP-1 cells. As for in vivo relevance, G-CSF dramatically reduced the weight loss of mice, colonic damage, and mucosal ulceration that characterize TNBS colitis. Moreover, G-CSF suppressed the expression of tumor necrosis factor-${\alpha}$, interleukin-$1{\beta}$, and intercellular adhesion molecule-1 in TNBS colitis. Conclusion: Current results demonstrate that G-CSF may be an effective agent for the treatment of diseases characterized by mucosal inflammation.

• Applied Microscopy
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• v.39 no.3
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• pp.253-260
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• 2009

GM-CSF is a multipotent growth factor, which also plays an important role during the process of wound healing. rrhGM-CSF was specifically produced from rice cell culture in our laboratory (Hanson Biotech Co., Ltd, Daejeon). The rrhGMCSF contains more oligosaccharide side chains than any other types of GM-CSF. This work was taken to evaluate the influence on wound healing of rrhGM-CSF in male golden hamsters. Full thickness skin defects of 9 mm in diameter were made in the back of hamsters, and 100 ${\mu}L$ ointment containing rrhGM-CSF 50 ${\mu}g/mL$ was applied. Control groups were given ointment without rrhGM-CSF. The wound sizes were relatively reduced and skin was well regenerated in the experimental group compared with the control group. Structurally, reepithelialization and architecture of the skin following injury were well accomplished in the experimental group. And also, positive reaction of PCNA of the skin following injury was more prominent in rrhGM-CSF containing ointment treatment group. Since this type of GM-CSF has highly glycosylated side chains, the effectiveness might be retain longer and stable, regarding acceleration of wound healing in the animal model. The present study has important implications for further development of the therapeutic manipulation of wound healing using rrhGM-CSF.

• Proceedings of the Korean Society of Computer Information Conference
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• 2018.07a
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• pp.389-390
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• 2018

본 논문에서는 3차원 복원을 위한 특징점 추출 및 매칭에 대한 보다 정확한 방법을 제안한다. 이 방법은 컴퓨터 비전의 기본이 되는 분야로 복원뿐 만 아니라 SLAM과 같은 지도 작성 및 자율 운행에도 필요한 방법이다. 본 연구는 3차원 물체 복원을 위해서 사용하는 방법 중 하나인 Column space fitting(CSF)을 이용하여 turntable-image data에 적용하여 성능을 평가하여 정확성을 검증을 한다. 오늘날 3D scanner를 이용하여 물체를 3차원 모델을 획득하고 3D프린터를 이용하여 다양한 분야에 적용한다. 그러나 고가의 장비이기 때문에 접근성이 떨어진다. 본 연구는 영상들만을 가지고 기하학적 계산을 통해 3차원 모델을 획득한다. 본 연구결과는 기존의 방법인 KLT 알고리즘과 비교하여 RMSE의 값을 약 5배를 줄이는 성능 향상을 보인다.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.35 no.7
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• pp.723-733
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• 2011

A numerical method of the CSF(Continuum Surface Force) model is presented for the calculation of the surface tension force and implemented in an in-house solution code(PowerCFD). The present method(code) employs an unstructured cell-centered method based on a conservative pressure-based finite-volume method with volume capturing method(CICSAM) in a volume of fluid(VOF) scheme for phase interface capturing. The application of the present method to a 2-D liquid drop problem is illustrated by an equilibrium and nonequilibrium oscillating drop calculation. It is found that the present method simulates efficiently and accurately surface tension-dominant multiphase flows.

• Korean Journal of Construction Engineering and Management
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• v.8 no.6
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• pp.227-234
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• 2007

현재 플랜트 산업이 해외 건설 부문에서 차지하는 비중은 해외 건설 수주액의 60% 이상에 달하고 있다. 하지만 시공부문의 높은 기술경쟁력에 비하여 고부가가치 창출이 가능한 설계부문은 그 기술이 미흡한 실정이다. 따라서 본 연구에서는 플랜트산업의 우수설계능력 확보를 위하여 플랜트 설계단계의 성공에 대한 정의를 내리고, 플랜트 설계업무를 분석한 뒤, 설문조사를 통하여 플랜트 설계단계 프로세스의 업무별 중요도를 파악하였다. 또한 이렇게 파악된 중요도 점수를 공정관리 프로그램에 적용하였고, 이를 바탕으로 제시된 결과에 대한 전문가의 검토를 통하여 최종적으로 플랜트 설계단계의 핵심성공요인(CSF)을 도출하였다. 본 연구의 결과는 플랜트 설계단계 성공의 요인을 파악하고 확인 및 관리를 위한 요소들을 제시하여 설계단계 업무수행의 가이드라인으로 활용될 수 있을 것으로 기대된다. 또한, 향후에는 도출된 핵심성공요인을 이용하여 플랜트의 성공과 실패를 가름할 수 있는 정량적 평가모델 개발 등의 연구가 수행될 수 있을 것이다.

• Biomolecules & Therapeutics
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• v.5 no.4
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• pp.376-379
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• 1997

The peripheral neutrophil recovery test was conducted to determine the efficacy of CJ-50001, a drug developed in Cheil Jedang R&D center as a recombinant granulocyte-colony stimulating factor (rG-CSF). Grasin was used as control drug. CJ-50001 and Gratin were subcutaneously administered to ${\gamma}$-ray irradiated mice for 21 days at a dose of 10$\mu$g/kg after bone marrow transplantation and the recovery of neutrophil number was examined on the days of 9, 13, 17, and 21 after the drug administration. It was observed that the peripheral neutrophil number of the vehicle control group was recovered to the normal level on the day of 13 after the transplantation whereas the group administered with CJ-50001 and Grasin respectively, showed the normal level of peripheral neutrophil number on 9th day after the bone marrow transplantation. The number of peripheral neutrophils reached the highest level on the 21 st day of drug administration, and was recovered to the normal level on the 4th day after ceasing of the drug administration (on the 25th day of the transplantation). Thus, it was presumed that CJ-50001 showed efficacy similar to Grasin on the peripheral neutrophil recovery after bone marrow transplantation.

• Applied Microscopy
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• v.40 no.2
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• pp.73-80
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• 2010

Liver regeneration is a result of highly coordinated proliferation of hepatocytes and nonparenchymal liver cells. Partial hepatectomy (PH) is the most often used stimulus to study liver regeneration because, compared with other methods that use hepatic toxins, it is not associated with the tissue injury and inflammation, and the initiation of the regenerative stimulus is precisely defined. Granulocyte macrophage-colony stimulating factor (GM-CSF), which is a cytokine able to regulate the proliferation and differentiation of epithelial cells, was first identified as the most potent mitogen for bone marrow. Particularly, rrhGM-CSF, which is highly glycosylated and sustained longer than any other types of GM-CSF in the blood circulation, was specifically produced from rice cell culture. In this experiment, effects of rrhGM-CSF administration were evaluated in the regenerating liver after 78% PH of rats. Morphological changes induced by PH were characterized by destroyed hepatocyte plate around the central vein and enlarged nuclear cytoplasmic ratio and increased hepatocytes with two nuclei. And then, proliferation of liver cells (parenchymal and nonparenchymal) and rearrangement of plates and lobules seemed to be carried out during liver regeneration. These alterations in the experimental group preceded those of the control. Since proliferating cell nuclear antigen (PCNA) is known to be a nuclear protein maximally elevated in the S phase of proliferating cells, the protein was used as a marker of liver regeneration after PH in rats. PCNA levels by western blot analysis and immunohistology were compared between the two groups. PCNA protein expression of two groups at 12 hr and 24 hr after injury showed similar pattern. The protein expression showed the peak at 3 days in both groups, however, the protein level of the experimental group was higher than that of the control. On immunohistochemical observations, the reaction product of PCNA was localized at the nuclei of proliferating cells and the positive reaction in experimental group at 3 days was clearly stronger than that in control group. The results by Western blotting and immunohistology for PCNA showed similar pattern in terms of the protein levels. In conclusion, rrhGM-CSF administration during liver regeneration after 78% PH accelerated breakdown and restoration of the hepatic plate and expression of PCNA. These results suggest that rrhGM-CSF might play an important role during liver regeneration in rats.

• Korean Journal of Head & Neck Oncology
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• v.19 no.1
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• pp.25-33
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• 2003

Background and Objectives: The Herpes Simplex type 2 Defective Infectious Single Cycle virus (DISC virus) is attenuated virus originally produced as viral vaccines but are also efficient gene transfer vehicle. The main goals of this study were to examine the efficiencies of the gene transfer using DISC vectors for various head and neck squamous cell carcinoma cell lines and to evaluate the efficacy of vaccination with DISC virus carrying a immunomodulatory genes (GM-CSF) as cancer therapy in a organotopic oral cavity squamous cell cancer model. Materials and Methods : We determinated the gene transfer efficiency of DISC virus by x-gal stain method and proved gene and protein expression of DISC-GMCSF transfected SCCVII cells by RT-PCR and ELISA method. Also we evaluated the ex vivo vaccination effects of SCCVII/GMCSF (DISC-GMCSF transfected SCCVII vaccine) vaccine on preventing the recurrence of micro-residual tumor. After the vaccination of SCCVII/GMCSF, specific cytotoxic T-cell responses was evaluated by CTL assay. Results: At an MOI of 10 DISC virus showed 64-88% of transfection rates in various head and neck squamous cancer cell lines. SCCVII cells transduced by DISC virus vector (MOI=10) carrying the GM-CSF gene, produced 4.5 nanogram quantities of GM-CSF per $10^6$ cells. In vivo vaccination using tumor cells transduced ex vivo with DISC-GMCSF resulted in better protection rate against subsequent tumor recurrence in organotopic oral cavity cancer model. Although tumor free survival rate was not statistically significantly increased in vaccination group (p=0.078), tumor specific cytotocic T-cell responses were significantly increased in SCCVII/GMCSF vaccination group. Conclusion: These data demonstrate that; 1) The DISC virus vector is capable of efficient gene transfer to various head and neck squamous cancer cell lines, 2) GM-CSF secreting genetically modified tumor vaccine (SCCVII/GMCSF) efficiently protected against tumor recurrence in organotopic micro-residual oral cavity cancer model and produced tumor specific cytotoxic T-cell response. DISC virus-mediated, cytokine gene transfer may prove to be useful as a clinical therapy for head and neck cancers.

• The Journal of Korean Institute for Practical Engineering Education
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• v.4 no.1
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• pp.86-92
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• 2012

For the successful operation of IPP program which is a unique Korean Co-op education program designed and implemented by Korea Tech, it is very crucial to have both a reasonable performance evaluation system and a systematic feedback and upgrading system for the program. In this paper, we will provide the logic model for long-term performance evaluation of Korea Tech's IPP program. Since the critical success factors(CSF) and Key Performance Index(KPI) are very important for the IPP program implementation, they are also provided and discussed in detail. In addition, we will discuss and analyze about the student and the industry survey results for IPP program's sucess factors.