• 대한임상검사과학회지
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• 제52권1호
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• pp.18-27
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• 2020

카바페넴내성장내세균속균종(carbapenem-resistant Enterobacteriaceae, CRE)과 카바페넴분해효소 생성 장내세균과(carbapenemase-producing Enterobacteriaceae, CPE)의 정확한 구분과 CPE의 빠른 탐지는 임상 감염의 치료 및 관리에 중요하다. 선별방법은 주로 선택적 배지에서의 직장 면봉 표본 배양 후 카바페넴분해 효소의 활성도, 신속한 카바페넴의 불활성화 방법, 측방유동면역분석(lateral flow immunoassay, LFI), 메트릭스보조레이저 탈착/이온화이온사이클론 공명 질량분석법(matrix assisted laser desorption/ionisation time of flight mass spectrometry, MALDI-TOF MS)을 통해 표현형을 측정하는 분자기반 방법들이다. CRE, 특히 CPE의 적절한 시기에 정확한 탐지는 감염의 임상 치료 및 예방에 필수적이다. 다양한 표현형 검출방법 및 유전자-기반 검출방법이 카바페넴의 신속한 검출을 위해 이용 가능하며, 이들은 임상 미생물학 실험실에서 일상적으로 사용된다. 신속한 처리 시간으로 현장에서 치료를 위한 검사 방법을 사용하는 CRE에 대한 능동적인 감시활동에서 카바페넴분해효소를 생성하는 CRE의 탐지는 중요한 가치를 갖는다. 따라서 카바페넴분해효소의 확산을 통제하기 위해서는 전세계의 많은 검사실에서 신뢰할 수 있고 신속하고 고효율적이며, 간편하고 저비용의 검사법을 사용해야 할 것이다. 환자의 적용에서도 최적의 효과를 가지려면 CRE에 대한 신속한 검사를 통해 항균제의 관리 개입이나 다양한 형태의 임상 의사의 치료에 결정적인 지원을 재현성있게 나타나야 할 것이다. 최적의 검사법을 위해서는 보완되는 검사법을 결합하여 다양한 내성 박테리아 종을 감별하고 다양한 종류의 카바페넴분해효소의 유전적 다양성을 발굴하여 최상의 감염관리 전략을 포괄하는 시스템이 마련되어야 할 것으로 사료된다.

• Journal of Yeungnam Medical Science
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• 제7권2호
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• pp.79-87
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• 1990

Using Zung's self-rating depression scale, the authors studied depression in 310 elderlies residing in the 9 home for the aged (HFA) in Kyungbuk area, and 234 elderlies living in Taegu city and 107 elderlies attending a Life-long education program in Taegu, which serve as a control group, Community resident elderlies(CRE). The research had been administered during the period from June to August, 1986. The results were as follows : 1. The mean scores of male. female. and the total of the elderlies in HFA were $38.95{\pm}11.55$, $44.18{\pm}14.15$:: and $42.8{\pm}13.7$ respectively while CRE marked $40.8{\pm}11.3$ for male, $45.2{\pm}12.0$ for female, and $43.4{\pm}11.9$ for the total. Therefore there were significant differences between male and female in both groups(p<0.01, p<0.001), though no significant difference between the two groups. However, the depression score of elderlies in HFA might be much higher than that of CRE if 81 elderlies(14.2%) in HFA who had been left out of the statistical evaluation due to their having severe depression, organic brain syndrom, or pseudoementia, etc had been included. 2. The score distribution by items for the elderlies in HFA were from highest scores hopelessness, worthlessness, emptiness, decreased appetite, confusion, while for CRE, hopelessness, decreased appetite, psychomotor retardation, indecisiveness, and worthlessness. Elderlies in HFA showed significantly high scores in depressed mood, weight loss, suicidal rumination(ideation) and psychomotor excitement, while CRE showed significantly high scores in decreased appetite, psychomotor retardation, indecisiveness, and dissatisfaction. 3. Elderlies who scored over 50 numbered 10 males(16%), 57 females(34%), and total of 67(29%) in HFA and 28 males(21%), 77 females(37%), and total of 105(31%) in CRE : female showed higher seores in both groups. 4. Psychosocial factors such as getting older(respectively p<0.01, p<0.01), being Buddhist(respectively p<0.01, p<0.01), and monthly pocket money less than \30,000(respectively p<0.001, p<0.001) were found to have a noticable impact on the depression level of the elderlies in both groups. Factors such as illiteracy (p<0.001, monthly pocket money less than \10,000(p<0.05), and having no family(p<0.01) recorded significantly higher scores among CRE than the elderlies in HFA.

• 한국식품저장유통학회지
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• 제23권5호
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• pp.631-637
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• 2016

커피박의 식품소재화를 위해 생리활성과 양갱 제조시 활용 가능성을 검토하기 위해 커피박 열수추출물 첨가 양갱(CREY)와 커피박 분말 첨가 양갱(CREP)를 제조하였다. 커피원두와 커피박 열수 추출물의 총 폴리페놀 함량과 DPPH 라디칼 소거능은 각각 $13.65{\mu}g/mL$와 78.75 %를 나타내었다. pH는 대조구가 7.42, CREY는 7.10~7.29의 범위를 나타내었고, CRPY는 6.95~7.15의 범위를 나타내었다. CREY의 수분 함량은 37.88~38.92%, CRPY는 39.3~40.0%의 범위로 대조구(36.34%)보다 높은 수분함량을 나타내었다. 당 함량은 대조구가 가장 높았다. 총 폴리페놀 함량은 CREY가 $15.88{\sim}66.19{\mu}g/mL$, CRPY는 $12.09{\sim}23.80{\mu}g/mL$의 범위를 나타내어 CRE와 CRP의 첨가농도(0.1, 0.3, 0.5 및 1.0 %)에 따라 유의적으로 증가하였다(p<0.05). DPPH 라디칼 소거능은 대조구가 1.88%, CREY는 8.77~43.10%로 농도 의존적으로 증가하였으며, CRYP도 5.28~14.92%의 범위를 나타내어 대조구보다 유의적으로 높았다(p<0.05). 양갱의 관능검사 결과, 색, 맛과 풍미 모두 CREY와 CRPY가 대조구에 비해 우수하였으며, 커피박 열수 추출물을 사용할 경우 0.5%, 커피박 분말을 사용할 경우 0.3%를 첨가한 양갱이 관능적으로 가장 우수하였다.

• 대한본초학회지
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• 제33권6호
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• pp.35-42
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• 2018

Objectives : The aim of this study was to investigate whether the extract of Coptidis Rhizoma inhibits inflammation in chronic cold stress (CCS)-exposed mice or not. Methods : Coptidis Rhizoma extract (CRE) was made by reflux with distilled water. Male ICR mice (7 weeks old) were divided randomly into 5 groups: (1) control, (2) CCS, (3) CCS+CRE 100 mg/kg, (4) CCS+CRE 300 mg/kg, (5) CCS+CRE 1,000 mg/kg groups. Mice were orally administered once a day for 14 days starting from 1 day before CCS. Group (2)-(5) were exposed to CCS conditions that maintained at $4^{\circ}C$ for 2 h once a day for 14 days. The levels of serum cortisol and hypothalamic prostaglandin E1 (PGE1) and PGE2 were measured by enzyme-linked immunosorbent assay kit. The expression levels of several pro-inflammatory factors like heat shock protein 70 (HSP70), c-fos, and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) were measured by western blot analysis in mouse hypothalamus. Results : Oral administration of CRE 1,000 mg/kg significantly suppressed the increase of serum cortisol levels in mice exposed to CCS. CCS-exposed mice had significantly increased the expression of HSP70, c-fos, and NF-kB in hypothalamus, while CRE treatment significantly attenuated the elevation of these pro-inflammatory factors. The ratio of PGE2/PGE1 was also higher in CCS-exposed mice than control group. CRE treatment significantly reduced the increase of PGE2/PGE1 ratio induced by CCS. Conclusion : These findings suggest that Coptidis Rhizoma may work as a potential agent to modulate inflammatory responses under the condition of cold adaptation formed by CCS.

• Journal of Microbiology and Biotechnology
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• 제25권7호
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• pp.1101-1107
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• 2015

The role of CRE1 in a thermophilic fungus, Myceliophthora thermophila ATCC42464, was studied using RNA interference. In the cre1-silenced strain C88, the filter paper hydrolyzing activity and β-1,4-endoglucanase activity were 3.76-, and 1.31-fold higher, respectively, than those in the parental strain when the strains were cultured in inducing medium for 6 days. The activities of β-1,4-exoglucanase and cellobiase were 2.64-, and 5.59-fold higher, respectively, than those in the parental strain when the strains were cultured for 5 days. Quantitative reverse-transcription polymerase chain reaction showed that the gene expression of egl3, cbh1, and cbh2 was significantly increased in transformant C88 compared with the wild-type strain. Therefore, our findings suggest the feasibility of improving cellulase production by modifying the regulator expression, and an attractive approach to increasing the total cellulase productivity in thermophilic fungi.

• Preventive Nutrition and Food Science
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• 제11권4호
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• pp.268-272
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• 2006

Cimicifugae Rhizoma (CR) belongs to the Ranunculaceae family, which has been traditionally used to treat climacteric complaints, antipyretics and diaphoresis as an alternative medicine for estrogen hormone replacement therapy with estrogens. Recently, it has been reported that different extract fractions of CR have various effects such as anti-allergic, anti-inflammatory and anti-proliferative activities. The current study investigated the immunomodulatory effects of Cimicifugae Rhizoma water extracts (CRE) in the macrophage-like cell line, Raw 264.7. Our results showed that CRE ($1\sim50\;{\mu}g/mL$) stimulated tumoricidal activity and NO production, whereas phagocytic activity was inhibited at the same concentrations. Additionally, iNOS mRNA expression was significantly increased in Raw 264.7 exposed to CRE as demonstrated by RT-PCR. These results indicate that the tumoricidal activity induced by CRE may be mediated by the production of NO and these activities may be useful for the treatment of diseases such as cancer.

• 한국통신학회논문지
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• 제25권10B호
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• pp.1675-1689
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• 2000

We present CReMeS a CORBA-compliant design and implementation of a new real-time communication service. It provides for efficient predictable and scalable communication between information producers and consumers. The CReMeS architecture is based on MidART's Real-Time Channel-based Reflective Memory (RT-CRM) abstraction. This architecture supports the separation of QoS specification between producer and consumer of data and employs a user-level scheduling scheme for communicating real-time tasks. These help us achieve end-to-end predictability and allows our service to scale. The CReMeS architecture provides a CORBA interface to applications and demands no changes to the ORB layer and the language mapping layer. Thus it can run on non real-time Off-The-Shelf ORBs enables applications on these ORBs to have scalable and end-to-end predictable asynchronous communication facility. In addition an application designer can select whether to use an out-of-band channel or the ORB GIOP/IIOP for data communication. This permits a trade-off between performance predictability and reliability. Experimental results demonstrate that our architecture can achieve better performance and predictability than a real-time implementation of the CORBA Even Service when the out-of-band channel is employed for data communication it delivers better predictability with comparable performance when the ORB GIOP/IIOP is used.

• Journal of Crop Science and Biotechnology
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• 제10권2호
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• pp.73-78
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• 2007

The green revolution has significantly helped in increasing the food production. So far, various breeding methods have been exploited, besides them recombination DNA technology provides another approach for increasing the food production. By means of this technology the losses in food production incurred by various biotic and abiotic stresses can be effectively controlled. In most of the transgenic studies scientists have used antibiotic resistant genes as markers for easy selection of transformants but there are risks involved in use of GM foods. To make such foods safer and environment friendly we have discussed a novel strategy i.e. Cre-lox which involves site specific recombination. By means of Cre-lox the marker genes can be specifically removed once the selection of transformants is over. In addition, this strategy can be used to module the hybrid chromosomes, avoid gene silencing and incorporate single copy of a transgene for its higher expression.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제7권11호
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• pp.2577-2596
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• 2013

In this paper, we present an Energy-Aware Self-Stabilizing Distributed Clustering protocol based on message-passing model for Ad Hoc networks. The latter does not require any initialization. Starting from an arbitrary configuration, the network converges to a stable state in a finite time. Our contribution is twofold. We firstly give the formal proof that the stabilization is reached after at most n+2 transitions and requires at most $n{\times}log(2n+{\kappa}+3)$ memory space, where n is the number of network nodes and ${\kappa}$ represents the maximum hops number in the clusters. Furthermore, using the OMNeT++ simulator, we perform an evaluation of our approach. Secondly, we propose an adaptation of our solution in the context of Wireless Sensor Networks (WSNs) with energy constraint. We notably show that our protocol can be easily used for constructing clusters according to multiple criteria in the election of cluster-heads, such as nodes' identity, residual energy or degree. We give a comparison under the different election metrics by evaluating their communication cost and energy consumption. Simulation results show that in terms of number of exchanged messages and energy consumption, it is better to use the Highest-ID metric for electing CHs.

• 구강회복응용과학지
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• 제30권3호
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• pp.223-230
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• 2014

목적: 하악 절치 발치 치료를 받은 환자들의 치료로 인한 향상과 유지기간 후의 안정성을 평가하는 것이다. 연구 재료 및 방법: 하악 절치를 발치한 환자 총 20명을 대상으로 치료 전, 치료 직후, 2년의 유지기간 후의 모형 및 파노라마 방사선 사진을 분석하였다. Irregularity index, overjet, overbite, 치아 크기와 악궁 길이의 차이, 견치간 폭경, 제1 대구치간 폭경 및 미국교정학회에서 제시한 방법(ABO-CRE)을 평가하였다. 통계분석은 Wilcoxon signed-rank test를 이용하였다. 결과: 치료 전후 irregularity index가 유의하게 감소되었고(P = 0.000), 치아 크기와 악궁 길이의 차이는 유의하게 증가 하였다(P = 0.028). 유지기간 동안에 irregularity index는 유의하게 악화 되었다(P = 0.001). ABO-CRE 전체값의 변화는 치료 전후에 유의한 감소를 보이고(P = 0.000), 유지 전후에는 유의한 차이를 보이지 않았다(P = 0.053). 결론: 모형과 파노라마 방사선 사진을 이용하여 치료의 안정성 평가 결과, 하악 절치 발치 치료는 2년간의 유지기간 동안 치료 결과가 안정적으로 유지되었다고 할 수 있다.