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http://dx.doi.org/10.6116/kjh.2018.33.6.35.

Anti-inflammatory effects of Coptidis Rhizoma in chronic cold stress-exposed mice  

Choi, Jin Gyu (Department of Pharmacy, College of Pharmacy, Kyung Hee University)
Huh, Eugene (Department of Medical Science of Meridian, Graduate School, Kyung Hee University)
Lee, Wonil (Department of Life and Nanopharmaceutical Sciences, Graduate school, Kyung Hee University)
Kim, Yun-Kyung (Department of Herbal Medicine, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University)
Lee, Tae-Hee (Department of Formulae Pharmacology, School of Oriental Medicine, Gachon University)
Oh, Myung Sook (Department of Life and Nanopharmaceutical Sciences, Graduate school, Kyung Hee University)
Publication Information
The Korea Journal of Herbology / v.33, no.6, 2018 , pp. 35-42 More about this Journal
Abstract
Objectives : The aim of this study was to investigate whether the extract of Coptidis Rhizoma inhibits inflammation in chronic cold stress (CCS)-exposed mice or not. Methods : Coptidis Rhizoma extract (CRE) was made by reflux with distilled water. Male ICR mice (7 weeks old) were divided randomly into 5 groups: (1) control, (2) CCS, (3) CCS+CRE 100 mg/kg, (4) CCS+CRE 300 mg/kg, (5) CCS+CRE 1,000 mg/kg groups. Mice were orally administered once a day for 14 days starting from 1 day before CCS. Group (2)-(5) were exposed to CCS conditions that maintained at $4^{\circ}C$ for 2 h once a day for 14 days. The levels of serum cortisol and hypothalamic prostaglandin E1 (PGE1) and PGE2 were measured by enzyme-linked immunosorbent assay kit. The expression levels of several pro-inflammatory factors like heat shock protein 70 (HSP70), c-fos, and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) were measured by western blot analysis in mouse hypothalamus. Results : Oral administration of CRE 1,000 mg/kg significantly suppressed the increase of serum cortisol levels in mice exposed to CCS. CCS-exposed mice had significantly increased the expression of HSP70, c-fos, and NF-kB in hypothalamus, while CRE treatment significantly attenuated the elevation of these pro-inflammatory factors. The ratio of PGE2/PGE1 was also higher in CCS-exposed mice than control group. CRE treatment significantly reduced the increase of PGE2/PGE1 ratio induced by CCS. Conclusion : These findings suggest that Coptidis Rhizoma may work as a potential agent to modulate inflammatory responses under the condition of cold adaptation formed by CCS.
Keywords
Coptidis Rhizoma; chronic cold stress; inflammation;
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