• Title/Summary/Keyword: CPE

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Molecular Analysis of Carbapenem-Resistant Enterobacteriaceae at a South Korean Hospital

  • Lee, Miyoung;Choi, Tae-Jin
    • Microbiology and Biotechnology Letters
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    • v.48 no.3
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    • pp.389-398
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    • 2020
  • The prevalence of carbapenem-resistant Enterobacteriaceae (CRE) is increasing globally, resulting in high mortality rates. Although CRE is a relatively recent problem in Korea (the first case was not diagnosed until 2010), it is responsible for serious morbidities at an alarming rate. In this study, we carried out a molecular genetic analysis to determine the incidence of CRE and carbapenemase-producing Enterobacteriaceae (CPE) at a general hospital in Korea between August 2017 and August 2019. Forty strains of CPE were isolated from various clinical specimens and analyzed via antimicrobial susceptibility testing, polymerase chain reaction to detect β-lactamase genes, deoxyribonucleic acid sequencing, multilocus sequence typing, curing testing, and conjugal transfer of plasmids. The results demonstrated that all 40 isolates were multidrug-resistant. The fluoroquinolone susceptibility test showed that 75% of the Enterobacteriaceae isolates were resistant to ciprofloxacin, whereas 72.5% were resistant to trimethoprim-sulfamethoxazole. Further, conjugation accounted for 57.5% of all resistant plasmid transfer events, which is 4.3-fold higher than that observed in 2010 by Frost et al. Finally, the high detection rate of transposon Tn4401 was associated with the rapid diffusion and evolution of CPE. Our results highlight the rapid emergence of extensively drugresistant strains in Korea and emphasize the need for employing urgent control measures and protocols at the national level.

Voltammetric Determination of Droxidopa in the Presence of Tryptophan Using a Nanostructured Base Electrochemical Sensor

  • Yaghoubian, Halimeh;Jahani, Shohreh;Beitollahi, Hadi;tajik, Somayeh;Hosseinzadeh, Rahman;Biparva, Pouria
    • Journal of Electrochemical Science and Technology
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    • v.9 no.2
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    • pp.109-117
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    • 2018
  • A novel carbon paste electrode modified with $Cu-TiO_2$ nanocomposite, 2-(ferrocenylethynyl)fluoren-9-one (2FF) and ionic liquid (IL) (2FF/$Cu-TiO_2$/IL/CPE) was fabricated and employed to study the electrocatalytic oxidation of droxidopa, using cyclic voltammetry (CV), chronoamperometry (CHA) and differential pulse voltammetry (DPV) as diagnostic techniques. It has been found that the oxidation of droxidopa at the surface of modified electrode occurs at a potential of about 295 mV less positive than that of an unmodified CPE. DPV exhibits a linear dynamic range from $5.0{\times}10^{-8}$ to $4.0{\times}10^{-4}M$ and a detection limit of 30.0 nM for droxidopa. Finally this modified electrode was used for simultaneous determination of droxidopa and tryptophan. Also the 2FF/$Cu-TiO_2$/IL/CPE shows excellent ability to determination of droxidopa and tryptophan in real samples.

Performance of Spectrum Sensing Using AF Cooperative Relay for Cognitive Radio System (인지 무선 통신에서 AF 협력 릴레이를 이용한 스펙트럼 센싱 성능)

  • Lee, Mi-Sun;Kim, Yoon-Hyun;Kim, Jin-Young
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.12 no.4
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    • pp.31-36
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    • 2012
  • In this paper, we proposed spectrum sensing using cooperative relay to solve problem of sensing performance degradation due to CPE (Customer-Premises equipments) which causes low SNR (signal-to-noise ratio) problem. In cooperative communication system, AF (amplify-and-forward) and DF (decoded-and-forward) is widely used for relay mechanism. Also, it is expected that cooperative relay scheme guarantees the high sensing performance by its diversity gain. Based on these backgrounds, in this paper, we apply to cooperative relay scheme to the CR (Cognitive Radio) system, and simulation results show comparison of the sensing performance.

Development of the EM Wave Absorber for the Hi-pass Using Amorphous Powder (비정질 재료를 이용한 Hi-pass용 전파흡수체 개발)

  • Yoo, Gun-Suk;Kim, Dong-Il;Choi, Dong-Soo
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.20 no.6
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    • pp.562-569
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    • 2009
  • Recently, Hi-pass system(non-stop electric toll collection) using DSRC is working. However, it has various problems such as system errors by EM wave interference or multi-path reflection. The EM wave absorber is able to solve these problems. In this paper, we designed and fabricated EM wave absorber using amorphous substance. As a result EM wave absorber with composition of amorphous metal powder:CPE=45:55 wt.% has the thickness of 2.65 mm and absorption ability was higher than 40 dB at 5.8 GHz.

A Protein Kinase-A Inhibitor, KT5720, Suppressed Cytopathic Effect Caused by Vesicular Stomatitis Virus (Protein Kinase Inhibitor, KT5720의 VSV에 의한 세포변성 억제 연구)

  • Kim, Young-Sook
    • Journal of Life Science
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    • v.17 no.10
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    • pp.1361-1367
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    • 2007
  • I investigated the effect of KT5720, an inhibitor of protein kinase A, on the vesicular stomatitis virus (VSV) infection in BHK-21cell cultures. The virus inducted cytopathic effect (CPE) was almost completely suppressed by KT5720 at 5uM. The inhibitor, however, did not affect replication of the virus nor the synthesis of viral macromolecules. KT5720, did not block the cytoskeletal disruption, while the cell rounding was suppressed. And, the KT5720-sensitive function may be involved in developing the VSV-induced CPE, but not essential for the virus replications.

Performance of Spectrum Sensing Using Cooperative Relay for Cognitive Radio System (인지 무선 통신을 위한 협력 릴레이 센싱 성능)

  • Lee, Mi-Sun;Kim, Yoon-Hyun;Kim, Jin-Young
    • Journal of Satellite, Information and Communications
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    • v.7 no.1
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    • pp.7-12
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    • 2012
  • In this paper, we proposed spectrum sensing using cooperative relay to solve problem of sensing performance degradation due to CPE (Customer-Primise equipments) which causes low SNR (signal-to-noise ratio) problem. In cooperative communication system, AF (amplify-and-forward) and DF (decoded-and-forward) is widely used for relay mechanism. Also, it is expected that cooperative relay scheme guarantees the high sensing performance by its diversity gain. Based on these backgrounds, in this paper, we apply to cooperative relay scheme to the CR (cognitive radio) system, and simulation results show comparison of the sensing performance between AF and DF.

Histopathological and immunohistochemical studies on the intestinal lesions related to the infected age in spontaneous canine parvovirus enteritis (개 파보바이러스장염의 감염일령에 따른 병변의 병리조직학적 및 면역조직화학적 관찰)

  • Ku, Ja-Iok;Seo, Il-bok;Lim, Chang-hyeong
    • Korean Journal of Veterinary Research
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    • v.34 no.3
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    • pp.537-547
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    • 1994
  • Ninety seven cases of histopathologically diagnosed spontaneous canine parvovirus enteritis(CPE) were studied gross pathologically, histopathologically, immunohistochemically, to investigate histopathological types of small intestinal lesions, and antigen distributions in each pattern related to the infected age. And also, reliability of histopathological method in diagnosis of CPE was inspected with immunohistochemistry. The results were as follows : 1. Age-related occurring ratio in histopathologically diagnosed CPE was 53.6% in 4-8 weeks, 26.8% in 9-15 weeks, 8.25 in 16-19 weeks and 11.3% in 20-45 weeks of the clog age. 2. In histopathologic classification based on patterns of villi/crypts lesions of small intestine(jejunum), the ratio of A type (initial phase of necrosis of crypt epithelia, desquamated epithelial cells in the dilated lumen of the crypt) was 20.6%; the ratio of B type(middle phase of atrophy and fission of the villi, collapse of the mucosa, loss of normal crypt structure) was 62.9%, and C type(regenerative phase of the crypt architecture) was 16.5%. 3. The ratio of A, B, C type in 4-8 weeks old, respectively, was 23.5%, 61.5%, 15.4%; in 9-15 weeks old was 19.2%, 65.4%, 15.3% in 16-19 weeks old was 25.0%, 75.0%, 0.0%; and in 20-45 weeks old was 9.0%, 54.5%, 36.4%. 4. The antigen distribution in the nuclei of the crypt epithelial cells was higher than of the cytoplasm and numerous desquamated epithelial cells in dialated crypts in A type; The antigen cytoplasm and numerous desquamated epithelial cells in dialated crypts in A type; The antigen distribution in the nuclei of the collapsed crypt epithelial cells was not higher than that of the cytoplasm, crypts were lined by and filled with released viral antigens from the destructed epithelial cells in B type; and its distribution was also higher than in the epithelial cells adjacent to the tips of the villi, but it was not reacted in the regenerative crypt epithelial cells in C type. 5. Immunohistochemically detected antigen ratio in the small intestine of histopathologically diagnosed CPE was 94.6%, and this result indicates that histopathological diagnosis is very reliable method in diagnosis of CPE.

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Development of the Construction Post-evaluation System in Public Construction Projects (공공건설사업에서의 건설공사 사후평가시스템 구축에 관한 연구)

  • Ok, Hyun;Yang, Sung-Hoon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.15 no.12
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    • pp.7364-7371
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    • 2014
  • Construction post-evaluation (CPE) boosts the efficiency of public construction projects by assessing the performance results of public construction projects and using the results to pursue similar projects. This evaluation comprehensively analyzes and assesses the planning of construction work, the estimated and actual construction costs, the construction period, the demand for construction projects, and the effects of construction projects after the construction work to ensure efficiency. The data was used to prevent lax project execution and enhance the quality and efficiency of similar construction projects. On the other hand, CPE results information must be managed systematically to refer to and use CPE data in similar construction projects. Therefore, in this study, the CPE system was developed as a measure of the systematic and comprehensive management and use of the CPE results information of individual ordering agencies. Therefore, the groundwork was laid for the assurance of quality enhancement and efficient project execution in public construction projects. This system is expected to serve as a useful tool for comprehensively analyzing and assessing construction works.

Determination of Hg (II) Ion at a Chemically Modified Carbon Paste Electrode Containing L-Sparteine (L-Sparteine 수식전극을 사용한 Hg (II) 이온의 정량)

  • Euh Duck Jeong;Mi-Sook Won;Yoon-Bo Shim
    • Journal of the Korean Chemical Society
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    • v.35 no.5
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    • pp.545-552
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    • 1991
  • A mercury ion-sensitive carbon-paste electrode (CPE) was constructed with l-sparteine. Mercury (II) ion was chemically deposited by the complexation with l-sparteine onto the CPE. The surface of CPEs was characterized by cyclic voltammetry and anodic stripping voltammetry in an acetate buffer solution, separately. Exposure of CPEs to acid solution could regenerate surface and reuse it for deposition. In 5 deposition/measurement/regeneration cycle, the response was reproducible and in licnear up to $2.0\;{\times}\;10^{-6}$ M with linear sweep voltammetry. In case of using the differential pulse technique, we have obtained the linear response up to $7.0 {\times}10^{-7}$ M with relative standard deviation of ${\pm}5.1$%. The detection limit was $5.0{\times}10^{-7}$ M for 20 minutes of the deposition. We have investigated the interference effect of various metal ions, which are expected to form the complex with ligand. Silver (I) ion of these has interfered with the analysis of Hg (II) ions. However, pretreatment of the silver (I) ion with potassium chloride led to no interference on the analysis of mercury ions in aqueous solution.

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A Study on EM Wave Absorber for Electromagnetic Wave Environment of Wireless LAN at 5.2 GHz (5.2 GHz 무선 LAN의 전자파 환경 대책용 전파흡수체에 관한 연구)

  • Yoo, Gun-Suk;Choi, Dong-Soo;Kim, Dong-Il
    • Journal of Navigation and Port Research
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    • v.34 no.1
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    • pp.15-19
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    • 2010
  • Recently, the wireless LAN system is rapidly growing because of its convenience of high speed communication. However, the wireless LAN systems at indoor places occur multi-propagation path by reflected waves from walls, ceilings, floors, and desks. Multipath problems cause transmission errors and degradation of communication speed. These problems can be solved by using EM wave absorbers. In this paper, we analyzed property of Graphite and derived the optimum ratio of Graphite: CPE to develop EM wave absorber for the wireless LAN system. First, we fabricated several samples in different composition ratios of Graphite and CPE, and then measured the reflection coefficient of each samples. Material constants of permittivity and permeability were calculated using the measured data and designed EM wave absorber. Secondly, the EM wave absorber was fabricated and tested on the base of the simulation data. As a result, it showed that the EM wave absorber in 1.7 mm thickness with the ratio of Graphite: CPE=50:50 wt.% has excellent absorption ability more than 27 dB at 5.2 GHz.