• 동의생리병리학회지
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• 제19권6호
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• pp.1640-1646
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• 2005

An oriental medicinal drugs Jahageo (JHG, Hominis placenta) were examined to determine its effects on the responsiveness of central nervous system neurons after injury. We found that JHG was involved in neurite outgrowth of DRG sensory axons. JHG treatment also increased expression of axonal growth-associated protein GAP-43 in DRG sensory neurons after sciatic nerve injury and in the injured spinal cord. JHG treatment during the spinal cord injury increased induction levels of cell division cycle 2 (Cdc2) protein in DRG as well as in the spinal cord. Histochemical investigation showed that induced Cdc2 in the injured spinal cord was found in non-neuronal cells. These results suggest that JHG regulates activities of non-neuronal cells such as oligodendrocyte and astrocyte in responses to spinal cord injury and protects neuronal responsiveness after axonal damage.

• Elastomers and Composites
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• 제42권2호
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• pp.75-85
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• 2007

본 연구에서는 폴리에스터 코드와 고무 매트릭스간의 접착을 향상시키기 위한 접착시스템에 관해 조사하였다. 폴리에스터 코드의 표면 활성화를 통해 접착력을 증진시키기 위해 0.03, 0.05, 0.1, 0.2, 0.5, 1 및 5 wt.％ NaOH 용액으로 폴리에스터 코드를 개질하였다. 개질된 폴리에스터 코드와 결합제가 직접블렌딩된 SBR 고무혼합물과의 접착력을 평가한 결과 0.05 wt.％의 NaOH 농도에서 10분 개질 할 때 최대의 접착강도를 나타내었다. 1 wt.％ 이상의 NaOH 농도에서는 과도한 개질로 인한 폴리에스터 코드의 손상으로 인하여 접착력 실험 동안 코드의 파괴가 발생하였다. 또한, 개질한 폴리에스터 코드에 triallylcyanurate(TC) 접착제를 코팅한 후 상온에서 건조하여 접착시키는 경우 보다 $220^{\circ}C$의 고온에서 건조한 후 고무 매트릭스와 접착시킬 경우 가장 우수한 접착력을 나타내었다.

• Restorative Dentistry and Endodontics
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• 제26권5호
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• pp.436-442
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• 2001

Neuropeptide such as calcitonin gene-related peptide and substance P may mediate neurogenic inflammation, but little is known about the regulation of neuropeptide release from rat spinal cord. Eugenol has been reported to reduce odontogenic pain and is known to have a structure similar to capsaicin, a potent stimulant of certain nociceptors. This study was done to examine the effect of capsaicin and eugenol on immunoreactive calcitonin gene-related peptide (iCGRP) release from rat spinal cord and whether eugenol regulates capsaicin-sensitive release of iCCRP or it evokes capsaicin-sensitive release of iCGRP. The dor-sal half of rat lumbar spinal cord was chopped into 200$\mu$m slices. They were superfused (500$\mu$l/min) in vitro with an oxygenated Kreb's buffer. The EC$_{50}$ of capsaicin on iCGRP release was measured. Eugenol (600$\mu$M and 1.2mM) and vehicle (0.02% 2-hydroxyl-$\beta$-cyclodextrin) were administered prior to stimulation of rat lumbar spinal cord with capsaicin. The amount of iCGRP release from rat lumbar spinal cord was measured by radioimmunoassay. The results were as follows : 1. iCGRP release from rat lumbar spinal cord was dependent on concentration of capsaicin. The EC$_{50}$ of capsaicin on iCGRP release was 3$\mu$M. 2. In the vehicle treated group, capsaicin (3$\mu$M) evoked a 14-fold increase over basal iCGRP level. 3. Administration of 600$\mu$M and 1.2mM eugenol evoked a 2.2-fold increase and a 2.3-fold increase over basal iCGRP level respectively. 4. Administration of 600$\mu$M and 1.2mM eugenol increased capsaicin evoked release of iCGRP by more than 50%. These results indicate that eugenol evoke CGRP release from central nervous system and potentiate the pain-inducing action of capsaicin on it.

• 한국전문물리치료학회지
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• 제14권2호
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• pp.11-20
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• 2007

The development of neonatal neuromuscular system is accomplished by the functional interaction between the spinal neurons and its target cells, skeletal muscle cells, and the intrinsic and extrinsic factors affecting this process. The aim of this study was to identify the effect of suspension unloading (SU) and neuromuscular electrical stimulation (NMES) upon the development of the neonatal spinal cord. For this study, the neonatal rats were randomly divided into three groups: a control group, an experimental group I, and an experimental group II. The SU for experimental group I and II was applied from postnatal day (PD) 5 to PD 30, and the NMES for experimental group II was applied from PD 16 to PD 30 using NMES that gave isometric contraction with 10 Hz for 30 minutes twice a day. In order to observe the effect of SU and NMES, this study observed neutrophin-3 (NT-3) and microtubule associated protein 2 (MAP2) immunoreactivity in the lumbar spinal cord (L4-5) at the PD 15 and PD 30. The results are as follows. At PD 15, lumbar spinal cord of experimental group I and II had significantly lower NT-3 and MAP2 immunoreactivity than control group. It proved that a microgravity condition restricted the spinal development. At PD 30, lumbar spinal cord of control group and experimental group II had significantly higher NT-3 and MAP2 immunoreactivity than experimental group I. It proved that the NMES facilitated the spinal development by spinal cord-skeletal muscle interaction. These results suggest that weight bearing during the neonatal developmental period is essential for the development of neuromuscular development. Also, the NMES on its target skeletal muscle can encourage the development of the spinal cord system with a full supplementation of the effect of weight bearing, which is an essential factor in neonatal developmental process.

• Journal of Nutrition and Health
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• 제33권8호
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• pp.840-847
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• 2000

This study was out to evaluate the folate nutritional status of Korean pregnant women and to investigate the relationship between serum folate levels of maternal-umbilical cord blood and pregnancy outcomes. Folate intakes of the pregnants was estimated by dietary folate intakes obtained from semiquantitative frequency questionnaire and supplementary folate intakes. The serum folate levels in both maternal blood and umbilical cord of 29 pregnant women at delivery and 13 nonpregnant controls were measured by redioimmunoassay. The total folate intakes(dietary and supplementary)of the pregnant women was 465. 4$\mu\textrm{g}$/d which was 93% of the Korean RDA for folate. Maternal mean serum folate levels of the pregnants was 6.1$\mu\textrm{g}$/ml, significantly lower than that of nonpregnant women(8.9ng/ml). Mean serumfolate level of umbilical cord blood was 14.2ng/ml, which was 2.3 folds higher than that of maternal blood. This finding indicates that the uptake of folate in the fetus may be due to an active placental transport mechanism. Maternal serum folate level correlated positively with that of umbilical cord blood, showing that folate concentration of umbilical cord blood is affected by maternal status. There was no significant correlation between the serum folate levels in maternal-umbilical cord blood and the pregnancy outcomes.(Korean J Nutrition 33(8) : 840-847, 2000)

• Journal of Korean Neurosurgical Society
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• 제50권4호
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• pp.288-292
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• 2011

Objective : Matrix metalloproteinases (MMPs), especially MMP-2 and MMP-9 have been known to play an important role in secondary inflammatory reaction after spinal cord injury (SCI). The aim of this study was to investigate the expression and activity of MMP-2 and MMP-9 and to determine their relationship with disruption of endothelial blood-barrier after photochemically induced SCI in rats. Methods : Female Sprague-Dawley rats, weighing between 250 and 300 g (aged 8 weeks) received focal spinal cord ischemia by photothrombosis using Rose Bengal. Expressions and activities of MMP-2 and MMP-9 were assessed by Western blot and gelatin zymography at various times from 6 h to 7 days. Endothelial blood-barrier integrity was assessed indirectly using spinal cord water content. Results : Zymography and Western blot analysis demonstrated rapid up-regulation of MMP-9 protein levels in spinal cord after ischemic onset. Expressions and activities of MMP-9 showed a significant increased at 6 h after the photothrombotic ischemic event, and reached a maximum level at 24 h after the insult. By contrast, activated MMP-2 was not detected at any time point in either the experimental or the control groups. When compared with the control group, a significant increase in spinal cord water content was detected in rats at 24 h after photothrombotic SCI. Conclusion : Early up-regulation of MMP-9 might be correlated with increased water content in the spinal cord at 24 h after SCI in rats. Results of this study suggest that MMP-9 is the key factor involved in disruption of the endothelial blood-barrier of the spinal cord and subsequent secondary damage after photothrombotic SCI in rats.

• Child Health Nursing Research
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• 제26권3호
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• pp.348-356
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• 2020

Purpose: This study aimed to identify correlations between the status of the umbilical cord and neonatal health status. Methods: In total, 172 newborns were enrolled who were admitted to the newborn nursery with a gestational age of 35 weeks or older and a body weight of 2 kg or above. Data were collected on the basic personal information of the newborns, the diameter and soft tissue status of the umbilical cord, and neonatal health status after birth. Analyses were performed using t-test, analysis of variance, χ² test, and Fisher exact test. Results: Umbilical cord diameter exhibited a statistically significant difference by sex (t=2.71, p=.007). A thin umbilical cord diameter was associated with a 1-minute Apgar score less than 8 points (t=2.47, p=.015) and with being transferred to the intensive care unit (t=2.45, p=.015). Poor soft tissue status of the umbilical cord was associated with a 1-minute Apgar score of less than 8 points (χ²=16.68, p<.001) and with oxygen being supplied (χ²=4.81, p=.028). Conclusion: Assessing the umbilical cord diameter and status in newborns is an important tool for evaluating neonatal health status after birth, and this point also underscores the importance of professionals' careful observations in the newborn nursery.

• 한방재활의학과학회지
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• 제23권2호
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• pp.1-15
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• 2013

Objectives : In present study, we investigated the effects of ginsenoside Rg3 on early-stage inflammatory response in spinal cord compression of rodents. Methods : Spinal cord injury(SCI) was induced by a vascular clip method(30 g, 5 min) on the spinal cord of mice. Rg3 was treated orally at 1 hour prior to the SCI induction. Messenger ribonucleic acid(mRNA) expression of tumor necrosis factor-${\alpha}$(TNF-${\alpha}$), interleukin-1${\beta}$(IL-1${\beta}$), interleukin-6(IL-6) and cyclooxygenase-2(COX-2) was measured by the real-time polymerase chain reaction(RT-PCR). Microglia in the spinal cord tissue, neurophils and COX-2 in the peri-lesion and inducible nitric oxide synthase(iNOS) expression in the ventral horn of SCI induced rats were measured by immunohistochemical stain. Results : 1. Rg3 significantly reduced the mRNA expression of TNF-${\alpha}$, IL-1${\beta}$, and COX-2 in the spinal cord tissue compared with SCI group(p<0.05, p<0.01). 2. Rg3 significantly reduced the total number of activated microglia and proportion of phagocytic form in the total activated microglia compared with SCI group(p<0.05, p<0.01). 3. Rg3 significantly reduced myeloperoxidase(MPO) positive neurophil in the peri-lesion compared with SCI group(p<0.05). 4. Rg3 reduced the COX-2 expression in the tissue and motor neurons compared with SCI group. 5. Rg3 significantly reduced iNOS positive motor neurons in the ventral horn compared with SCI group(p<0.01). Conclusions : In conclusion, we demonstrated at first that treatment of ginsenoside Rg3 could reduce significantly the levels of inflammatory mediators in a spinal cord compression model of rodents. Therefore, these results suggested that ginsenoside Rg3 may be a useful antimiflamatory therapeutic candidate for SCI.

• Journal of Nutrition and Health
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• 제31권8호
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• pp.1263-1269
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• 1998

The maintenance of adequate folate levels in the umbilical cord blood is esential for supplying tissue requirements of fetal growth. However, there is data on folate levels in the cord blood of Korean infant. The present investigation was undertaken to determine folate levels in cord blood and aassess relationships between folate levels and pregnancy outcomes. Dietary and supplementary folate intake was obtained from thirty subjects who were in the third trimester fo pregancy . The umbilical cord blood was drawn at delivery and pregnancy outcomes for the subjects were collected from their medical records. Erythrocyte and plasma folate levels in the cord blood were analyzed. The subjects were divided into two groups ; high folate (HF, $\geq$654ng/ml) and low folate (LF, <654ng/ml) groups according to erythrocyte folate levels in cord blood. Dietary folate intake and the amount of supplemental folates were not significantly different between the two experimental groups. However, infant birth weight (3540$\pm$295g) and placental weight(910$\pm$85g) for the HF group were significantly higher(p=0.0041 and p=0.109, respectively) than those for the LF group, which were 3127 $\pm$419g and 823$\pm$80g , respectively. Although it was not significant, the gestational weight gain for the HF group was 2.8kg higher than that for the LF group. Thus, the erythrocyte folate level in the cord blood was significantly related to infant birth weight and placental weight. These results confirm that a high erythrocyte folate level in the umbilical cord blood promotes both fetal and placental growth and improves gestational weight gain as well.

• 한국화재소방학회:학술대회논문집
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• 한국화재소방학회 2003년도 춘계학술논문발표회논문집
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• pp.164-170
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• 2003

In this paper, we studied on the firing characteristics and electrical fire analysis of power cord deteriorated by thermal stress. The cross section of PVC insulating cord deteriorated by indirect flame decreased through heat convection. PVC insulating cord deteriorated by direct flame was bumpy shape. The exothermic peak of normal cord was shown at ($526.7^{\circ}C$), but the peaks or on(heat treatment temperature) ($150^{\circ}C$) cord was shown at ($299.6^{\circ}C$) and [$502.2^{\circ}C$]. The exothermic peaks according to high temperature were similar to those of amorphous carbon. In the FT-IR analysis, the absorption peak of normal cord indicated double bond of oxygen and carbon in benzene ring at 1720.0$cm^{1}$. As the HTT was high, the height of characteristic peak decreased and the peak of carbonyl group was shown at about 1625.7$cm^{-1}$. The characteristic peak of single bond(O-H) was shown at about 3479.2$cm^{-1}$. In case of the internal part of wire covering deteriorated by over current, the characteristic peak were shown at about 3417.3$cm^{-1}$ and 1600.2$cm^{-1}$. The above results show that we can distinguish the differences according to the fire pattern through the internalㆍexternal analysis of wire covering deteriorated by heat.