Browse > Article

Effects of Oriental Medicinal Drugs on Axonal Regeneration in the Spinal Cord Neurons  

An Joung-Jo (Department of Internal Medicine, College of Oriental Medicine, Daejeon University)
NamGung Uk (Department of Neurophysiology, College of Oriental Medicine, Daejeon University)
Seo In-Chan (Department of Internal Medicine, College of Oriental Medicine, Daejeon University)
Kim Yoon-Sik (Department of Internal Medicine, College of Oriental Medicine, Daejeon University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.19, no.6, 2005 , pp. 1640-1646 More about this Journal
Abstract
An oriental medicinal drugs Jahageo (JHG, Hominis placenta) were examined to determine its effects on the responsiveness of central nervous system neurons after injury. We found that JHG was involved in neurite outgrowth of DRG sensory axons. JHG treatment also increased expression of axonal growth-associated protein GAP-43 in DRG sensory neurons after sciatic nerve injury and in the injured spinal cord. JHG treatment during the spinal cord injury increased induction levels of cell division cycle 2 (Cdc2) protein in DRG as well as in the spinal cord. Histochemical investigation showed that induced Cdc2 in the injured spinal cord was found in non-neuronal cells. These results suggest that JHG regulates activities of non-neuronal cells such as oligodendrocyte and astrocyte in responses to spinal cord injury and protects neuronal responsiveness after axonal damage.
Keywords
Hominis placenta; regeneration; spinal cord; DRG sensory neuron; GAP-43; Cdc2;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Schwab, M.E. Repairing the injured spinal cord. Science 295, 1029-1031, 2002.
2 Horner, P,J., Gage, F.H. Regenerating the damaged central nervous system. Nature 407, 963-970, 2000.
3 Fournier, AE., GrandPre, T., Strittmatter, S.M. Identification of a receptor mediating Ncigo-66 inhibition of axonal regeneration. Nature 409, 341-346, 2001.
4 GrandPre, T., Nakamura, F., Vartanian, T., Strittmatter, S.M. Identification of the Nogo inhibitor of axon regeneration as a Reticulon protein. Nature 403, 439-444, 2000.
5 Wang, K.C., Koprivica, V., Kim, J.A, Sivasankaran, R., Guo, Y., Neve, R.L., He, Z. Oligodendrocyte-myelin glycoprotein is a Nogo receptor ligand that inhibits neurite outgrowth. Nature 417, 941-944, 2002.
6 Haller, C.A., Benowitz, N.L. Adverse cardiovascular and central nervous system events associated with dietary supplements containing ephedra alkaloids. N. Engl. J. Med. 21, 1833-1838, 2000.
7 Corio, A., Vitadello, M. Ganglioside prevention of neuronal functional decay. Prog. Brain. Res. 71, 203-208, 1987.
8 Cheng, Y.S., Cheng, W.C, Yao, C.H., Hsieh, C.L., Lin, J.G., Lai, T.Y., Lin, C.C., Tsai, C.C. Effects of buyang huanwu decoction on peripheral nerve regeneration using silicone rubber chambers. Am. J. Chin. Med. 29, 423-432, 2001.
9 Benowitz,L.I., Routtenberg, A GAP-43: an intrinsic determinant of neuronal development and plasticity. Trends. Neurosci. 20, 84-91, 1997.
10 Curt, A., Schwab, M.E., Dietz, V. Providing the clinical basis for new interventional therapies: refined diagnosis and assessment of recovery after spinal cord injury. Spinal Cord. 42, 1-6, 2004.
11 Bradbury, E.J., Moon, L.D., Popat, R.J., King, V.R., Bennett, G.S., Patel, P.N., Fawcett, J.W., McMahon, S.B. Chondroitinase ABC promotes functional recovery after spinal cord injury. Nature 11, 636-640, 2002.
12 Gong, X., Sucher, N.J. Stroke therapy in traditional Chinese medicine (TCM): prospects for drug discovery and development. Trends. Pharmacol. Sci. 20, 191-196, 1999.
13 GrandPre, T., Li. S., Strittmatter, S.M. Nogo-66 receptor antagonist peptide promotes axonal regeneration. Nature 417, 547-551, 2002.
14 Chen, Y.S., Wu, C.H., Yao, C.H., Chen, C.T. Ginsenoside RbI enhances peripheral nerve regeneration across wide gaps in silicone rubber chambers. Int. J. Artif. Organs. 25, 1103-1108, 2002.
15 Neumann, S., Woolf, CJ. Regeneration of dorsal column fibers into and beyond the lesion site following adult spinal cord injury. Neuron 23, 83-91, 1999.
16 Schreyer, D,J., Skene, J.H. Injury-associated induction of GAP-43 expression displays axon branch specificity in rat dorsal root ganglion neurons. J. Neurobiol. 24, 959-970, 1993.
17 Chen, MS., Huber, A.B., van der Haar, M.E., Frank, M., Schnell, L., Spillmann, A.A., Christ, F., Schwab, M.E. Nogo-A is a myelin-associated neurite outgrowth inhibitor and an antigen for monoclonal antibody IN-1. Nature 403, 434-439, 2000.
18 Park, J. Safety and efficacy needs to be tested in an appropriate design. Am. J. Chin. Med. 31, 1001-1002, 2003.
19 Fawcett, J.W., Asher, RA. The glial scar and central nervous system repair. Brain. Res. Bull. 49, 377-391, 1999.
20 McGee, A.W., Strittmatter, S.M. The Nogo-66 receptor: focusing myelin inhibition of axon regeneration. Trends. Neurosci. 26, 193-198. 2003.
21 Bomze, H.M., Bulsara, K.R., Iskandar, B.J., Caroni, P., Skene, J.H. Spinal axon regeneration evoked by replacing two growth cone proteins in adult neurons. Nat. Neurosci. 4, 38-43, 2001.
22 전국한의과대학 본초학교수 共編著. 本草學. 永林社. pp 155-156, 287-288, 331-333, 545-546, 562-563, 567-568, 571-572, 서울, Korea, 2000.
23 Porter, L.A., Donoghue, D.J. Cyclin B1 and CDK1: nuclear localization and upstream regulators. Prog. Cell. Cycle. Res. 5, 335-347, 2003.
24 Doree, M., Galas, S. The cyclin-dependent protein kinases and the control of cell division. FASEB. J. 8, 1114-1121. 1994.
25 Woolf, c.J., Bloechlinger, S. Neuroscience. It takes more than two to Nogo. Science 297, 1132-1134, 2002.
26 Kim, W.H., Choi, W.Y. 장부변증논치, 서울, 성보사, pp 281-287, 1990.
27 Corio, A, Aporti, F., Di Gregorio, F., Schiavinato, A, Siliprandi, R, Vitadello, M. Ganglioside treatment of genetic and alloxan-induced diabetic neuropathy. Adv. Exp. Med. BioI. 174, 549-564, 1984.
28 Namgung, U., Routtenberg, A. Transcriptional and post-transcriptional regulation of a brain growth protein: regional differentiation and regeneration induction of GAP-43. Eur. J. Neurosci. 12, 3124-3136, 2000.