• Title/Summary/Keyword: COG

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Genetic Composition Analysis of Marine-Origin Euryarchaeota by using a COG Algorithm (COG 알고리즘을 통한 해양성 Euryarchaeota의 유전적 조성 분석)

  • 이재화;이동근;김철민;이은열
    • Journal of Life Science
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    • v.13 no.3
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    • pp.298-307
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    • 2003
  • To figure out the conserved genes and newly added genes at each phylogenetic level of Archaea, COG (clusters of orthologous groups of proteins) algorithm was applied. The number of conserved genes within 9 species of Archaea was 340 and that of 8 species of Euryarchaeota was 388. Many of conserved 265 COGs, which are specific to Archaea and absent in Bacteria and S. cerevisiae, were concerned with 'information storage and processing' (94 COG, 35.5%) and 'metabolism' (82 COG, 30.9%). COGs related to these functions were assumed as highly conserved and permit peculiar life form to Archaea. It seemed that there was some difference in 'nucleotide transport and metabolism' and there was little difference in 'information storage and processing' between Euryarchaeota and Crenarchaeota. Marine-origin Euryarchaeota showed different conserved COGs with terrestrial Euryarchaeota. Conserved COGs, related to carbohydrate transport and metabolism and others, were different between marine- and terrestrial-origin Euryarchaeota. Hence it was assumed that their physiology might be different. This study may help to understand the origin and conserved genes at each phylogenetic level of marine-origin Euryarchaeota and may help in the mining of useful genes in marine Archaea as Manco et al. (Arch. Biochem. Biophy. 373, 182 (2000)).

Kinematic Analyses of Men's Pole Vault in IAAF World Championships, Daegu 2011 (2011 대구 세계육상선수권대회 남자 장대높이뛰기경기 기술의 운동학적 분석)

  • Choi, Kyoo-Jeong;Yi, Kyung-Ok;Kim, Nam-Hee;Kang, Ji-Eun;Kim, Hye-Lim
    • Korean Journal of Applied Biomechanics
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    • v.21 no.5
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    • pp.551-560
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    • 2011
  • The purpose of this study was to perform the kinematic analyses of the men's pole vault skills in IAAF World Championships Daegu 2011. Subjects were the 1st through 8th place finishers in the pole vault. The kinematic analyses were divided into four phases: two dimensional run up analysis, and three dimensional analyses for the remaining plant, swing up, and extension phases. Run-up variables consisted of run up distance, number of steps, average step length, the ratio of step length to his height, average velocity at the final 6~11 m, approach position. Three variables were analyzed during plant: pole angle, center of gravity (COG) velocity, and takeoff angle of COG. Swing up phase variables included: pole flexion angle, COG velocity (horizontal, vertical, resultant), COG trajectory and bar approach angle of COG. Compared to the 2009 World Championships in Berlin, the average vault height, run up velocity and approach position increased. However, horizontal velocity during the last two steps of the final approach decreased dramatically compared to speeds from 1990. These results reflect the change in both technique and improved physical fitness in pole vaulters. During extension, the peak height of COG averaged 0.3m higher then COG height when the pole was released. These specific results can help coaches and athletes modify training and improve performance.

Phylogenetic analysis of procaryote by uridylate kinase (Uridylate kinase를 이용한 원핵생물의 분류)

  • 이동근;김철민;김상진;하배진;하종명;이상현;이재화
    • Journal of Life Science
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    • v.13 no.6
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    • pp.856-864
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    • 2003
  • The 16S rRNA gene is the most common gene in the phylogenetic analysis of procaryotes. However very high conservative of 16S rRNA has limitation in the discrimination of highly related organisms, hence other molecule was applied in this study and the result was compared with that of 16S rRNA. Three COGs (Clusters of Orthologous of protein) were only detected in 42 procaryotes ; transcription elongation facto. (COG0195), bacterial DNA primase (COG0358) and uridylate kinase (COG0528). Uridylate kinase gene was selected because of the similarity and one single copy number in each genome. Bacteria, belong to same genus, and Archaebacteria were same position with high bootstrap value in phylogenetic tree like the tree of 16S rRNA. However, alpha and epsilon Proteobcteria showed different position and Spirochaetales of Eubarteria was grouped together with Archaebacteria unlike the result of 16S rRNA. Uridylate kinase may compensate the problem of very high conservative of 16S rRNA gene and it would help to access more accurate discrimination and phylogenetic analysis of bacteria.

Hovering Performance Improvement by Modifying COG of Underwater Robotic Platform (수중운항로봇 플랫폼의 무게중심 조정을 통한 제어성능 향상)

  • Bak, Jeongae;Kim, Jong-Won;Jin, Sangrok;Kim, Jongwon;Seo, TaeWon
    • Journal of the Korean Society for Precision Engineering
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    • v.32 no.7
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    • pp.661-666
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    • 2015
  • This paper presents control performance improvement by modifying center of gravity (COG) of an underwater robotic platform. To reduce the oscillation or to increase the positioning accuracy, it is important to accurately know the COG of an underwater robotic platform. The COG is determined by the three measured tilting angles of the platform in different postures. The tilting angle is measured while the platform is hanged by two strings. Using coordinate transformation, the plane of intersection is defined from the angle of the platform and the position of the string. The COG of the robotic platform is directly calculated by the intersected point in three defined planes. The measured COG is implemented to the control algorithm that is pre-designed in the previous research, and the empirical result on tilting gives 48.26% improved oscillation performance comparing to the oscillation result with the ideal COG position.

Investigation for warpage in the COG Module process (COG Module 공정에서의 Glass 휨 연구)

  • Kim, Byoung-Yong;Seo, Dae-Shik
    • Proceedings of the IEEK Conference
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    • 2008.06a
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    • pp.515-516
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    • 2008
  • We studied about new module technology to solve warpage problems that produce bending of cell in the LCD (Liquid crystal display). Characteristics of cell gap and glass bending of applying heat Panel's PAD part and cell at various temperature was investigated. When applies heat and compresses PAD party only in case of compressing COG(Chip on Glass), uniformity of cell gap that happen by glass bending by temperature of these compressing COG in the PAD party is decreased.

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A COG Variable Analysis of Air-rolling-breakfall in Judo (유도 공중회전낙법의 COG변인 분석)

  • Kim, Eui-Hwan;Chung, Chae-Wook;Kim, Sung-Sup
    • Korean Journal of Applied Biomechanics
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    • v.15 no.3
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    • pp.117-132
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    • 2005
  • It was to study a following research of "A Kinematic Analysis of Air-rolling-breakfall in Judo". The purpose of this study was to analyze the Center of Gravity(COG) variables when performing Air-rolling-breakfall motion, while passing forward over(PFO) to the vertical-hurdles(2m height, take off board 1m height) in judo. Subjects were four males of Y. University squad, who were trainees of the demonstration exhibition team, representatives of national level judoists and were filmed by four 5-VHS 16mm video cameras(60field/sec.) through the three dimensional film analysis methods.COG variable were anterior-posterior directional COG and linear velocity of COG, vertical directional COG and linear velocity of COG. The data collections of this study were digitized by KWON3D program computed The data were standardized using cubic spline interpolation based by calculating the mean values and the standard deviation calculated for each variables. When performing the Air-rolling-breakfall, from the data analysis and discussions, the conclusions were as follows : 1. Anterior-posterior directional COG(APD-COG) when performing Air-rolling-breakfall motion, while PFO over to the vertical-hurdles(2m height) in judo. The range of APD-COG by forward was $0.31{\sim}0.41m$ in take-off position(event 1), $1.20{\sim}1.33m$ in the air-top position(event 2), $2.12{\sim}2.30m$ in the touch-down position(event 3), gradually and $2.14{\sim}2.32m$ in safety breakfall position(event 4), respectively. 2 The linear velocity of APD-COG was $1.03{\sim}2.14m/sec$. in take-off position(event 1), $1.97{\sim}2.22m/sec$. gradually in the air-top position(event 2), $1.05{\sim}1.32m/sec$. in the touch-down position (event 3), gradual decrease and $0.91{\sim}1.23m/sec$. in the safety breakfall position(event 4), respectively. 3. The vertical directional COG(VD-COG) when performing Air-rolling-breakfall motion, while PFO to the vertical-hurdles(2m height) in judo. The range of VD-COG toward upward from mat was $1.35{\sim}1.46m$ in take-off position(event 1), the highest $2.07{\sim}2.23m$ in the air-top position(event 2), and after rapid decrease $0.3{\sim}0.58m$ in the touch-down position(event 3), gradual decrease $0.22{\sim}0.50m$ in safety breakfall position(event 4), respectively. 4. The linear velocity of VlJ.COG was $1.60{\sim}1.87m/sec$. in take-off position(event 1), $0.03{\sim}0.08m/sec$. gradually in the air-top position(event 2), $-4.37{\sim}\;-4.76m/sec$. gradual decrease in the touch-down position(event 3), gradual decrease and -4.40${\sim}\;-4.77m/sec$. in safety breakfall position(event 4), respectively. When performing Air-rolling-breakfall showed parabolic movement from take-off position to air-top position, and after showed vertical fall movement from air-top position to safety breakfall. In conclusion, Ukemi(breakfall) is safety fall method Therefore, actions need for performing safety fall movement, that decrease and minimize shock and impact during Air-rolling-breakfall from take-off board action to air-top position must be maximize of angular momentum, and after must be minimize in touch-down position and safety breakfall position.

Phylogenetic Analysis of 680 Prokaryotes by Gene Content (유전자 보유 계통수를 이용한 원핵생물 680종의 분석)

  • Lee, Dong-Geun;Lee, Sang-Hyeon
    • Journal of Life Science
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    • v.26 no.6
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    • pp.711-720
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    • 2016
  • To determine the degree of common genes and the phylogenetic relationships among genome-sequenced 680 prokaryotes, the similarities among 4,631 clusters of orthologous groups of protein (COGs)’ presence/ absence and gene content trees were analyzed. The number of COGs was in the range of 103–2,199 (mean 1377.1) among 680 prokaryotes. Candidatus Nasuia deltocephalinicola str. NAS-ALF, an obligate symbiont with insects, showed the minimum COG, while Pseudomonas aeruginosa PAO1, an opportunistic pathogen, represented the maximum COG. The similarities between two prokaryotes were 49.30–99.78 % (mean 72.65%). Methanocaldococcus jannaschii DSM 2661 (hyperthermophilic and autotrophic, Euryarchaeota phylum) and Mesorhizobium loti MAFF303099 (mesophilic and symbiotic, alpha-Proteobacteria class) had the minimum amount of similarities. As gene content may represent the potential for an organism to adapt to each habitat, this may represent the history of prokaryotic evolution or the range of prokaryotic habitats at present on earth. COG content trees represented the following. First, two members of Chloroflexi phylum (Dehalogenimonas lykanthroporepellens BL-DC-9 and Dehalococcoides mccartyi 195) showed a greater relationship with Archaea than other Eubacteria. Second, members of the same phylum or class in the 16S rRNA gene were separated in the COG content tree. Finally, delta- and epsilon-Proteobacteria were in different lineages with other Proteobacteria classes in neighbor-joining (NJ) and maximum likelihood (ML) trees. The results of this study would be valuable to identifying the origins of organisms, functional relationships, and useful genes.

Uridylate kinase as a New Phylogenetic Molecule for Procaryotes

  • Lee, Dong-Geun;Lee, Jin-Ok;Lee, Jae-Hwa
    • 한국생물공학회:학술대회논문집
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    • 2003.10a
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    • pp.810-814
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    • 2003
  • For the phylogenetic analysis of procaryotes, 16S rRNA gene has been used. In spite of it's common use, so high conservative of 16S rRNA gene limited resolving power, hence other molecule was applied in this study and the result was compared with that of 16S rRNA. COG (Clusters of Orthologous of protein) algorithm revealed that three COGs were only detected in 42 procaryotes ; transcription elongation factor (COG0195), bacterial DNA primase (COG0358) and uridylate kinase (COG0528). Uridylate kinase gene was selected owing to the similarity and one single copy number in each genome. Phylogenetic tree of 16S rRNA gene and uridylate kinase showed similarities and differences. Uridylate kinase may help the problem of very high conservative of 16S rRNA gene in rhylogenetic analysis and it would help to access more accurate discrimination and phylogenetic analysis of bacteria.

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