• Korean Journal of Medicinal Crop Science
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• v.23 no.6
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• pp.439-445
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• 2015

Background : Correct identification of Panax species is important to ensure food quality, safety, authenticity and health for consumers. This paper describes a high resolution melting (HRM) analysis based method using internal transcribed spacer (ITS) and 5.8S ribosomal DNA barcoding regions as target (Bar-HRM) to obtain barcoding information for the major Panax species and to identify the origin of ginseng plant. Methods and Results : A PCR-based approach, Bar-HRM was developed to discriminate among Panax species. In this study, the ITS1, ITS2, and 5.8S rDNA genes were targeted for testing, since these have been identified as suitable genes for use in the identification of Panax species. The HRM analysis generated cluster patterns that were specific and sensitive enough to detect small sequence differences among the tested Panax species. Conclusion : The results of this study show that the HRM curve analysis of the ITS regions and 5.8S rDNA sequences is a simple, quick, and reproducible method. It can simultaneously identify three Panax species and screen for variants. Thus, ITS1HRM and 5.8SHRM primer sets can be used to distinguish among Panax species.

• International Commerce and Information Review
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• v.13 no.3
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• pp.249-274
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• 2011

This paper examines agri-food export competitiveness among 4 countries(Korea, USA, Japan, China) based on Porter's Diamond model approach. Korea agri-food competitiveness index is much less than those of other countries. Korea is the lowest ranked among the 4 countries in terms of three determinants(except 1 factor, related and supporting industries) of Porter's Diamond Model: Factor condition, demand condition, firm strategy, structure and rivalry. Korea is assumed to have weak point in the factor condition, demand condition because of low capital and land as well as insufficient investment on the agri-food sector. on the other hand, it seems to have strong factor in consumer condition(demand condition). This paper suggest the following strategies to reinforced the agri-food export sector in Korea. 1) an incentive system for seed development and capital investment, 2) a cluster strategy between agriculture and related industry.

• The Journal of the Korea Contents Association
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• v.14 no.11
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• pp.592-601
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• 2014

This study examines newspaper obituaries in Korean media and English media. Initially, 100 Korean obituaries were collected from the JoongAng Ilbo which span over more than three years, from May 2011 to August 2014. After that, another 50 Korean obituaries were gathered from the DongA Ilbo which were published over the same time period with the JoongAng Ilbo. As for English newspapers, obituaries from the New York Times and the Guardian were included in the corpus for comparison. First, the structure and composition of obituaries in each language (Korean and English) are compared. Korean obituaries show a pattern of a combination of a death notice and an obituary. Second, distinct features of each newspaper are discussed. The JoongAng Ilbo has its obituary section titled "Life and Memories", and the DongA Ilbo's obituaries are under the heading of "Rest in Peace." Obituaries in the New York Times appear in print on different pages of the paper according to the deceased's field of interest. Following discussion of formal structure and characteristics of each newspaper, Korean and English obituaries will be compared in terms of content and cultural context.

• Analytical Science and Technology
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• v.10 no.5
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• pp.362-369
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• 1997

Chemometric technique was applied to the sugar composition in five honeys of known botanical or geographical origin following HPLC. Fructose and glucose were predominant carbohydrates in honeys, and small amount of sucrose was also detected in one sample. Sugar contents in honeys samples were compared by the geographical or botanical origin. Fructose/glucose ratio ranged from 0.99 to 1.55 was obtained and these results are in good agreement with the ratio of literature. The plot of principal components analysis(PCA) showed that different honey samples grouped into distinct cluster by the geographical or botanical origin. Increasing the first or second principal component score, higher amount of sugar or less fructose/glucose ratio was observed in PCA plot. Chemometric approach was very useful to provide pattern recognition of sugar profile or quality indices of honey sample and to detect adulteration.

• World Technopolis Review
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• v.1 no.1
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• pp.56-64
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• 2012

This paper provides a case study to explore the Kitakyushu case as a good referential example on STP development for rejuvenation of declining industrial city. The major data for the case, basically, has been sourced from some materials published by the municipality of Kitakyushu City, the website of the Kitakyushu Science and Research Park (KSRP) etc. The City of Kitakyushu has promoted the KSRP development to overcome industrial decline and stimulate city renaissance as a new industrial city. The core of the development was to develop a high-level education and research environment by gathering several HEIs into one campus. Based on the environment, the Kitakyushu Foundation for the Advancement of Industry and Science and Technology (FAIS) played the critical role as a coupler to make various networks and boost industry-academiaadministration innovation. The KSRP, and then, has been designated as a project area of some cluster projects initiated by the central government. It means that local projects can be synchronized, synergized and synthesized with national projects in the KSRP. In addition, through a series of the environmental approach from the Kitakyushu Eco-town Project to the Low Carbon Society Project, the development of the KSRP is being extended into the region. In the Kitakyushu case, networking is the essence of the KSRP development from the planning stage. First of all, the plan put emphasis on networking of academia for the knowledge creation based on competitive collaboration that is expected as the mainspring of rejuvenating declining industrial area and making a new industrial city that the plan aimed. Then, the roles of two organizations are very important for networking, especially networking of networks: the Campus Management Committee as an interface and the FAIS as a coupler. STP development without some strategic considerations for networking as the way of interaction among its participants cannot be a tool to promote innovation and rejuvenate a declining industrial area. The Kitakyushu case mentioned above explains the matter clearly.

• Journal of Microbiology and Biotechnology
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• v.16 no.11
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• pp.1728-1733
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• 2006

A Gram-negative, strictly aerobic, nonmotile, and nonspore-forming bacterial strain, designated $T5-12^T$, was isolated from compost and characterized using a polyphasic taxonomical approach. The isolate was positive for catalase and oxidase tests. It could degrade DNA, but was negative for degradation of macromolecules such as casein, collagen, starch, chitin, cellulose, and xylan. The DNA G+C content was 36.0 mol%. The predominant isoprenoid quinone was menaquinone 7 (MK-7). The major fatty acids were $iso-C_{15:0}$ (45.6%), $iso-C_{17:0}$ 3OH (17.2%), and summed feature 4 ($C_{16:0}\;{\omega}7c$ and/or $iso-C_{15:0}$ 2OH, 14.9%). Comparative 16S rRNA gene sequence analysis showed that strain $T5-12^T$ fell within the radiation of the cluster comprising members of the genus Sphingobacterium. Strain $T5-12^T$ exhibited lower than 94% of 16S rRNA gene sequence similarity with respect to the type strains of recognized Sphingobacterium species. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain $T5-12^T$ ($=KCTC\;12578^T=LMG\;23401^T=CCUG\;52467^T$) should be classified in the genus Sphingobacterium as the type strain of a novel species, for which the name Sphingobacterium composti sp. novo is proposed.

• The Journal of the Korea Contents Association
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• v.9 no.5
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• pp.76-83
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• 2009

This paper presents a compression method for animated meshes or mesh sequences which have a shared connectivity and geometry streams. Our approach is based on static semi-regular mesh compression algorithm introduced by Khodakovky et al. Our encoding algorithm consists of two stages. First, the proposed technique creates a semi-regular mesh sequence from an input irregular mesh sequence. For semi-regular remeshing of irregular mesh sequences, this paper adapts the MAPS algorithm. However, MAPS cannot directly be performed to the input irregular mesh sequence. Thus, the proposed remesh algorithm revises the MAPS remesher using the clustering information, which classify coherent parts during the animation. The second stage uses wavelet transformation and clustering information to compress geometries of mesh sequences efficiently. The proposed compression algorithm predicts the vertex trajectories using the clustering information and the cluster transformation during the animation and compress the difference other frames from the reference frame in order to reduce the range of 3D position values.

• Journal of Microbiology and Biotechnology
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• v.25 no.7
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• pp.1015-1025
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• 2015

The development of diverse polymyxin derivatives is needed to solve the toxicity and resistance problems of polymyxins. However, no platform has generated polymyxin derivatives by genetically engineering a polymyxin synthetase, which is a nonribosomal peptide synthetase. In this study, we present a two-step approach for the construction of engineered polymyxin synthetases by substituting the adenylation (A) domains of polymyxin A synthetase, which is encoded by the pmxABCDE gene cluster of Paenibacillus polymyxa E681. First, the seventh L-threonine-specific A-domain region in pmxA was substituted with the L-leucine-specific A-domain region obtained from P. polymyxa ATCC21830 to make polymyxin E synthetase, and then the sixth D-leucine-specific A-domain region (A_6-D-Leu-domain) was substituted with the D-phenylalanine-specific A-domain region (A_6-D-Phe-domain) obtained from P. polymyxa F4 to make polymyxin B synthetase. This step was performed in Escherichia coli on a pmxA-containing fosmid, using the lambda Red recombination system and the sacB gene as a counter-selectable marker. Next, the modified pmxA gene was fused to pmxBCDE on the chromosome of Bacillus subtilis BSK4dA, and the resulting recombinant strains BSK4-PB and BSK4-PE were confirmed to produce polymyxins B and E, respectively. We also succeeded in constructing the B. subtilis BSK4-PP strain, which produces polymyxin P, by singly substituting the A_6-D-Leu-domain with the A_6-D-Phe-domain. This is the first report in which polymyxin derivatives were generated by genetically engineering polymyxin synthetases. The two recombinant B. subtilis strains will be useful for improving the commercial production of polymyxins B and E, and they will facilitate the generation of novel polymyxin derivatives.

• Journal of Communications and Networks
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• v.5 no.2
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• pp.157-166
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• 2003

This paper deals with the problem of channel identification for Single Input Multiple Output (SIMO) slow fading channels using clustering algorithms. Due to the intrinsic memory of the discrete-time model of the channel, over short observation periods, the received data vectors of the SIMO model are spread in clusters because of the AWGN noise. Each cluster is practically centered around the ideal channel output labels without noise and the noisy received vectors are distributed according to a multivariate Gaussian distribution. Starting from the Markov SIMO channel model, simultaneous maximum ikelihood estimation of the input vector and the channel coefficients reduce to one of obtaining the values of this pair that minimizes the sum of the Euclidean norms between the received and the estimated output vectors. Viterbi algorithm can be used for this purpose provided the trellis diagram of the Markov model can be labeled with the noiseless channel outputs. The problem of identification of the ideal channel outputs, which is the focus of this paper, is then equivalent to designing a Vector Quantizer (VQ) from a training set corresponding to the observed noisy channel outputs. The Linde-Buzo-Gray (LBG)-type clustering algorithms [1] could be used to obtain the noiseless channel output labels from the noisy received vectors. One problem with the use of such algorithms for blind time-varying channel identification is the codebook initialization. This paper looks at two critical issues with regards to the use of VQ for channel identification. The first has to deal with the applicability of this technique in general; we present theoretical results for the conditions under which the technique may be applicable. The second aims at overcoming the codebook initialization problem by proposing a novel approach which attempts to make the first phase of the channel estimation faster than the classical codebook initialization methods. Sample simulation results are provided confirming the effectiveness of the proposed initialization technique.

• Mycobiology
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• v.44 no.3
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• pp.155-161
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• 2016

The most economically important species used in a wide range of fermentation industries throughout Asia belong to Aspergillus section Flavi, which are morphologically and phylogenetically indistinguishable, with a few being toxigenic and therefore a major concern. They are frequently isolated from Korean fermentation starters, such as nuruk and meju. The growing popularity of traditional Korean alcoholic beverages has led to a demand for their quality enhancement, therefore requiring selection of efficient non-toxigenic strains to assist effective fermentation. This study was performed to classify the most efficient strains of Aspergillus section Flavi isolated from various types of traditional wheat nuruk, based on a polyphasic approach involving molecular and biochemical evaluation. A total of 69 strains were isolated based on colony morphology and identified as Aspergillus oryzae/flavus based on internal transcribed spacer and calmodulin gene sequencing. Interestingly, none were toxigenic based on PCR amplification of intergenic regions of the aflatoxin cluster genes norB-cypA and the absence of aflatoxin in the culture supernatants by thin-layer chromatography analysis. Saccharification capability of the isolates, assessed through ${\alpha}-amylase$ and glucoamylase activities, revealed that two isolates, TNA24 and TNA15, showed the highest levels of activity. Although the degrees of variation in ${\alpha}-amylase$ and glucoamylase activities among the isolates were higher, there were only slight differences in acid protease activity among the isolates with two, TNA28 and TNA36, showing the highest activities. Furthermore, statistical analyses showed that ${\alpha}-amylase$ activity was positively correlated with glucoamylase activity (p < 0.001), and therefore screening for either was sufficient to predict the saccharifying capacity of the Aspergillus strain.