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http://dx.doi.org/10.7783/KJMCS.2015.23.6.439

Internal Transcribed Spacer Barcoding DNA Region Coupled with High Resolution Melting Analysis for Authentication of Panax Species  

Bang, Kyong Hwan (Planning and Coordination Division, NIHHS, RDA)
Kim, Young Chang (Department of Herbal Crop Research, NIHHS, RDA)
Lim, Ji Young (Department of Herbal Crop Research, NIHHS, RDA)
Kim, Jang Uk (Department of Herbal Crop Research, NIHHS, RDA)
Lee, Jung Woo (Department of Herbal Crop Research, NIHHS, RDA)
Kim, Dong Hwi (Department of Herbal Crop Research, NIHHS, RDA)
Kim, Kee Hong (Department of Herbal Crop Research, NIHHS, RDA)
Jo, Ick Hyun (Department of Herbal Crop Research, NIHHS, RDA)
Publication Information
Korean Journal of Medicinal Crop Science / v.23, no.6, 2015 , pp. 439-445 More about this Journal
Abstract
Background : Correct identification of Panax species is important to ensure food quality, safety, authenticity and health for consumers. This paper describes a high resolution melting (HRM) analysis based method using internal transcribed spacer (ITS) and 5.8S ribosomal DNA barcoding regions as target (Bar-HRM) to obtain barcoding information for the major Panax species and to identify the origin of ginseng plant. Methods and Results : A PCR-based approach, Bar-HRM was developed to discriminate among Panax species. In this study, the ITS1, ITS2, and 5.8S rDNA genes were targeted for testing, since these have been identified as suitable genes for use in the identification of Panax species. The HRM analysis generated cluster patterns that were specific and sensitive enough to detect small sequence differences among the tested Panax species. Conclusion : The results of this study show that the HRM curve analysis of the ITS regions and 5.8S rDNA sequences is a simple, quick, and reproducible method. It can simultaneously identify three Panax species and screen for variants. Thus, ITS1HRM and 5.8SHRM primer sets can be used to distinguish among Panax species.
Keywords
Panax Species; High Resolution Melting; ITS Barcoding; Real Time PCR;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
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