• Journal of the Institute of Convergence Signal Processing
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• v.2 no.4
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• pp.91-96
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• 2001

In this paper we propose a new ENMODL(Enhanced-NORA-MODL) CLA(Carry-Look Ahead Adder) for high speed and low power multiplier. To reduce transistor counts, area and power dissipation we developed new-approaches. The method makes use of a dynamic CMOS logic ENMODL CLA. The advantage of ENMODL is small area and high speed The speed of ENMODL CLA is invreased by 6.27 % as compared with conventional NMOCL CLA. The proposed method was verified by HSPICE simulation and layout througth 0.6${\mu}{\textrm}{m}$ CMOS process.

• Food Science of Animal Resources
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• v.18 no.4
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• pp.339-347
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• 1998

The effects of conjugated linoleic acid (CLA) in egg yolk on fatty acid composition and lipid oxidation during chilled storage (4$^{\circ}C$) were investigated. CLA was synthesized according to the method of alkali isomerization using safflower seed oil (SSO). A total of 250 hens (200 days of age) were fed control diet (commercial formula feed for han) or CLA-supplemented diet (1%, 2.5% and 5% CLA) or 5% SSO supplemented diet for 6 weeks, and eggs were collected for analysis of CLA, fatty acid compositons and lipid oxidation. Eggs from CLA-supplemented diets groups showed significantly (p＜0.05) higher CLA content compared to those of control group. The contents of linoleic, palmitic, and myristic acid were increased as well as CLA content by feeding a CLA-supplemented diet. However, the contents of oleic and arachidonic acids in egg yolks were decreased by dietary CLA supplementation. The pH of egg yolk increased by the levels of CLA during storage. The contents of CLA were not significantly (p＜0.05) changed during chilled storage for 28 days, whereas TBARS were significantly (p＜0.05) increased. It is suggested that lipid oxidation of egg yolk might be affected by the levels of CLA in egg yolk due to changes in fatty acid compositions.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.3
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• pp.365-371
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• 2016

This study was aimed to evaluate the stability of conjugated linoleic acids (CLAs) by nano-encapsulation against in vitro ruminal biohydrogenation by microbial enzymatic conversion. CLAs (free fatty acid form of CLA [CLA-FFA], nano-encapsulated CLA-FFA, triglyceride form of CLA [CLA-TG], and nano-encapsulated CLA-TG) were used in the in vitro fermentation experiments. When Butyrivibrio fibrisolvens (B. fibrisolvens) was incubated with CLA-FFAs, the concentrations of cis-9, trans-11 CLA and vaccenic acid (VA) slightly was decreased and increased by nano-encapsulation, respectively. When B. fibrisolvens was incubated with CLA-TG, the concentrations of cis-9, trans-11 CLA and VA decreased, but these were increased when B. fibrisolvens was incubated with nano-encapsulated CLA-TG. The nano-encapsulation was more effective against the in vitro biohydrogenation activity of B.fibrisolvens incubated with CLA-FFA than with CLA-TG. In the in vitro ruminal incubation test, the total gas production and concentration of total volatile fatty acids incubated with nano-encapsulated CLA-FFA and CLA-TG were increased significantly after 24 h incubation (p<0.05). Nano-encapsulated CLA-FFA might, thus, improve the ruminal fermentation characteristics without adverse effects on the incubation process. In addition, nano-encapsulated CLA-FFA increased the population of Fibrobacter succinogenes and decreased the population of B. fibrisolvens population. These results indicate that nano-encapsulation could be applied to enhance CLA levels in ruminants by increasing the stability of CLA without causing adverse effects on ruminal fermentation.

• Korean Journal of Poultry Science
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• v.29 no.2
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• pp.125-134
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• 2002

Two experiments were conducted to compare the dietary supplemental influence of conjugated linoleic acid(CLA), soybean oil(SBO) and commercial tallow(CT) on MEn, performance and breast meat composition of broiler chicks. Diets contained 21.5, 19% CP and 3,100, 3,100kcal/kg ME for starter and finisher, respectively. Each three levels(1.0, 2.0, 3.0%) of CLA, SBO, CT were supplemented to basal diets. Five hundred fDrty and three hundred sixty one day old, male broiler chicks were replaced to 3$\times$3, 2$\times$3 factorial design with four replicates in Expt 1 and 2. Weight gain, fled intake, fled conversion, W antibody titer and fatty acid composition were measured. Metabolizable energy(ME) were measured through the metabolic feeding trial in each oil. ME was 8,542, 9,179, 8,733 kcal/kg in CLA, SBO and CT, respectively. In Expt 1, weight gain was not statistically different between dietary oil treatments. Feed intake was significantly increased by CLA supplement(P<0.05). Feed conversion was significantly improved in SBO supplemental groups of all treatments(P<0.05). Weight gain and feed intake were significantly increased and fled conversion was significantly improved in CLA 2% and 3% supplemental groups compared with CLA 1% group(P<0.05). Fatty acid composition of breast meat was changed by CLA supplement. CLA content of breast meat was 12.23, 18.74, 25.67 mg/g in 1, 2, and 3% CLA treatments and showed significant difference between them(P<0.05). In Expt 2, CLA supplements increased weight gain signi(icantly for (inishing period(P<0.05) compared to that of other treatments. There was no significant difference in M Antibody titer in Expt 1 and Expt 2. As the results of these experiments, birds fed CLA tended to gain higher weight and significantly increased CLA contents of breast meat(P<0.05).

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.1
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• pp.49-56
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• 2009

The objective was to determine the effects of supplementation of protected conjugated linoleic acid (CLA), CLA-20 comprising 10% each of cis-9, trans-11 and trans-10, cis-12, on milk production and fatty acid profiles in plasma and milk in lactating dairy cows. Five mid-lactation, multiparous crossbred Holstein Friesian cows with average 402${\pm}$20 kg BW were used in a 5${\times}$5 Latin square design for 21-d periods. Cows were given a total mixed ration (TMR) and supplemented with CLA-20 at 0, 20, 40, 80 and 160 g/d. The results showed that dry matter intake depression occurred in cows supplemented with CLA-20 at 160 g/d. Milk production slightly increased when CLA-20 supplementation was at 20, 40 and 80 g/d. However, 3.5% fat-corrected milk (FCM) was not affected by CLA-20 supplementation. Increased levels of CLA-20 supplementation resulted in a significantly decreased percentage of milk fat. Plasma concentrations of fatty acid were not altered by the amounts of CLA-20 supplementation except for the concentration of trans-10, cis-12 CLA. For all dietary treatments, percentages of fatty acids (C4:0, C6:0, C8:0, C13:0, C14:0 C14:1 C15:0 C15:1 C16:0, C16:1, C18:1n9t, C18:2n6t, C18:2n6c, C20:0, C18:3n6, C18:3n3, C20:1 and C20:3n6) in milk fat were similar. Concentrations of C10:0, C11:0, C12:0 and C18:1n9c were decreased cubically and C18:0 was elevated linearly (p<0.01) according to the increased amounts of CLA-20 supplemented. The linear increase was observed for cis-9, trans-11 CLA (0.62, 1.17, 1.94, 1.87 and 1.82% of total fatty acid), trans-10, cis-12 CLA (0.01, 0.63, 0.67, 0.93 and 0.95% of total fatty acid) and total CLA (0.80, 2.25, 3.16, 3.97 and 3.94% of total fatty acid) in milk fat from 0 to 160 g/d of CLA-20 supplement. In conclusion, concentration of cis-9, trans-11 CLA in milk fat was concomitantly elevated at an increasing rate with the increased amounts of CLA-20. Based on the results in this study, supplementation of CLA-20 at 80 g/d optimally enhanced total CLA in milk fat.

• Food Science of Animal Resources
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• v.21 no.4
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• pp.349-357
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• 2001

The effects of dietary conjugated linoleic aicd on pork quality was investigated using sixty four pigs. CLA was synthesized by alkaline isomerization method with corn oil. Pigs were devided into 4 treatment groups(16 pigs/group) arid subjected to one of four treatment diets(0, 0.1, 0.2, and 0.3% CLA diets) for 2 weeks before slaughter. Pork loin and belly were collected from the animals(105∼110kg body weight) slaughtering at the commercial slaughter house. Pork loin and belly meat were aerobic packaged and then stored during 2. 5. 8. 11 and 13 days at 4$\^{C}$ refrigerator. Samples were analysed for meat color, texture and sensory characteristics. There were no diference in meat color(L*, a*, b*), texture and sensory property among control and CLA treatment (s), and no changes as the storage period passed. This results mean that feeding CLA to pork doesn't change the meat color and texture of the pork what consumers consider when they buy some meat. Especially, feeding CLA to pork didn't transform sensory property of the pork. We considered these result as no problem in the pig fed with CLA.

• Korean Journal of Food Science and Technology
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• v.41 no.3
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• pp.307-312
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• 2009

To investigate the effects of conjugated linoleic acid (CLA) and/or exercise on body fat mass and weight, college women of normal weight (2130%) were recruited for this study. The participants were divided into 4 groups: placebo-no exercise, placebo-exercise, CLA-no exercise, and CLA-exercise groups. Three grams of either a placebo (corn oil) or CLA were taken every day for 12 weeks, and the exercise groups performed 80 minutes of aerobic and anaerobic exercise three times a week for 12 weeks. There were no differences in nutrient intakes among the groups. The CLA-exercise group had significantly lower body weight, BMI and body fat mass compared to the placebo-no exercise group. In addition, the HDL-cholesterol levels of subjects in the CLA-no exercise and CLA-exercise groups significantly increased compared to those in the placebo-no exercise group. These results suggest that a combination of CLA supplementation with exercise could efficiently reduce body fat mass and body weight compared to CLA supplementation or exercise alone.

• Journal of Nutrition and Health
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• v.34 no.4
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• pp.367-374
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• 2001

Conjugated linoleic acid(CLA) is a naturally occuring group of derivatives of linoleic acid found in beef and dairy products. CLA has reported to reduce body fat. This study was designed to observe the effect of CLA supplementation on fat accumulation and degradation in male Sprague Dawley rats. Seventy two rats, weighing 150-180g, were divided into 2 groups according to the types of dietary fat(beef tallow or fish oil) and then each group was divided into 2 groups depending on CLA supplementation, i.e., BT, BT-CLA, FO, FO-CLA. All rats were fed experimental diet containing total fat at 12%(w/w) including CLA at 1% for 30 weeks. At 30 weeks, rats were sacrificed to measure TG, free fatty acid level in plasma, TG, lipogenic enzymes in lever and fat cell size, LPL and HSL activities in epididymal fat fad. Fish oil supplemented with CLA diet showed significant reduction in the food efficiency and weight in 30 weeks-fed rats. CLA supplement did not effect on plasma TG, hepatic TG levels and lipogenic enzymes activities in rats, but, fish oil significantly reduced, The LPL and HSL activities did not affected by CLA supplement and n-3 fatty acid rich fish oil. In conclusion, the results suggest that CLA supplement was not a proper way to reduce the fat accumulation in Sprague Dawley rats. Fish oil supplemented with CLA might better way to reduce the body fatness than fish oil itself. Therefore, It is recommended that further study be performed related to physiological and biochemical effects of CLA supplementation and n-3 fatty acid in rats for the reduction of body fatness.(Korean J Nutrition 34(4) : 367∼374, 2001)

• Journal of Nutrition and Health
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• v.36 no.3
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• pp.280-286
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• 2003

Conjugated linoleic acid (CLA) consists of several geometric isomers of linoleic acid. CLA is found in foods derived from ruminants and exhibits strong anticarcinogenic effects in a variety of animal models. Matrix metalloproteinases (MMPs) play a key role in cancer progression. Specifically, MMP-2 and -9, which hydrolyze the basal membrane type IV collagen, are involved in the initial breakdown of collagen and basement membrane components during tumor growth and invasion. However, the effects of CLA on cancer cell motility and MMP expression and activity are not currently well known. Therefore, the present study examined whether CLA reduces the activity of MMP and cell motility in SW480 and SW620 cells, the human colon cancer cell lines. Gelatin zymography and Western blot analysis revealed that phorbol 12-myristate 13-acetate (PMA) induced the activity and protein expression of Mr 92,000 MMP-9 in both cell lines. To examine whether CLA inhibits the MMP activity, cells were incubated with 100 ngfmL PMA in the presence of various concentrations of CLA. PMA-induced MMP-9 activity was decreased by 20 $\mu$ M CLA in SW480 cells, and by 10 $\mu$ M and 20 $\mu$ M CLA in SW620 cells. Results from the Hoyden chamber assay showed that cell motility was increased by PMA and that PMA-induced cell motility was significantly decreased by 20 $\mu$ M CLA in SW480 cells. These results indicate that CLA may reduce the motility and MMP activity in human colon cancer cells.

• Journal of Animal Science and Technology
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• v.47 no.2
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• pp.195-204
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• 2005

The current study was conducted to investigate the effects of dietary conjugated linoleic acid(CLA) on growth performance and body fat metabolism in broiler chickens. A total of 48 male birds aged 3 days were randomly allotted into three dietary groups; CORN(com oil 1.5%), CLA I (com oil 0.75 %+ CLA 0.75 %) and CLA II(CLA 1.5%) groups. After feeding commercial diet ad libitum for 3 weeks, eight selected birds on the basis of body weight were housed, two birds in a cage, and continuously given ad libitum corresponding experimental diet for another 2 weeks. As a result, dietary addition of CLA did not influence on body weight, gain and feed conversion rote. The relative weights of the liver and deposited fats(abdominal and thigh fat' pads) were not also affected by the dietary treatments. Serum glucose, triglyceride and cholesterol levels markedly( P < 0.05) decreased in CLA II compared with those in CORN group. However, serum nonesterified fatty acid(NEFA) was not altered by dietary CLA. Serum leptin level was tended to be decreased by dietary CLA without statistical difference. The diet supplemented with CLA caused a significant(P< 0.05) decrease in hepatic total lipid and NEFA without changing triglyceride level. Also, feeding dietary CLA at the level of 1.5% reduced leptin mRNA expression in the liver and abdominal fats compared with feeding com oil, In conclusion, our results suggest that dietary 1.5% CLA may affect, at least in part, lipid metabolism in the liver of broiler chickens.