Browse > Article
http://dx.doi.org/10.5713/ajas.2009.70380

Effects of Protected Conjugated Linoleic Acid Supplementation on Milk Fatty Acid in Dairy Cows  

Piamphon, N. (Faculty of Agro-industrial Technology, Rajamangala University of Technology Isan Kalasin campus)
Wachirapakorn, Chalong (Department of Animal Science, Khon Kaen University)
Wanapat, M. (Department of Animal Science, Khon Kaen University)
Navanukraw, C. (Department of Animal Science, Khon Kaen University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.22, no.1, 2009 , pp. 49-56 More about this Journal
Abstract
The objective was to determine the effects of supplementation of protected conjugated linoleic acid (CLA), CLA-20 comprising 10% each of cis-9, trans-11 and trans-10, cis-12, on milk production and fatty acid profiles in plasma and milk in lactating dairy cows. Five mid-lactation, multiparous crossbred Holstein Friesian cows with average 402${\pm}$20 kg BW were used in a 5${\times}$5 Latin square design for 21-d periods. Cows were given a total mixed ration (TMR) and supplemented with CLA-20 at 0, 20, 40, 80 and 160 g/d. The results showed that dry matter intake depression occurred in cows supplemented with CLA-20 at 160 g/d. Milk production slightly increased when CLA-20 supplementation was at 20, 40 and 80 g/d. However, 3.5% fat-corrected milk (FCM) was not affected by CLA-20 supplementation. Increased levels of CLA-20 supplementation resulted in a significantly decreased percentage of milk fat. Plasma concentrations of fatty acid were not altered by the amounts of CLA-20 supplementation except for the concentration of trans-10, cis-12 CLA. For all dietary treatments, percentages of fatty acids (C4:0, C6:0, C8:0, C13:0, C14:0 C14:1 C15:0 C15:1 C16:0, C16:1, C18:1n9t, C18:2n6t, C18:2n6c, C20:0, C18:3n6, C18:3n3, C20:1 and C20:3n6) in milk fat were similar. Concentrations of C10:0, C11:0, C12:0 and C18:1n9c were decreased cubically and C18:0 was elevated linearly (p<0.01) according to the increased amounts of CLA-20 supplemented. The linear increase was observed for cis-9, trans-11 CLA (0.62, 1.17, 1.94, 1.87 and 1.82% of total fatty acid), trans-10, cis-12 CLA (0.01, 0.63, 0.67, 0.93 and 0.95% of total fatty acid) and total CLA (0.80, 2.25, 3.16, 3.97 and 3.94% of total fatty acid) in milk fat from 0 to 160 g/d of CLA-20 supplement. In conclusion, concentration of cis-9, trans-11 CLA in milk fat was concomitantly elevated at an increasing rate with the increased amounts of CLA-20. Based on the results in this study, supplementation of CLA-20 at 80 g/d optimally enhanced total CLA in milk fat.
Keywords
Protected Conjugated Linoleic Acid; Plasma; Fatty Acid; Milk; Cows;
Citations & Related Records

Times Cited By Web Of Science : 2  (Related Records In Web of Science)
Times Cited By SCOPUS : 2
연도 인용수 순위
1 AOAC. 1990. Official Methods of Analysis. $5^{th}$ ed. Association of Official Analytical Chemists, Arlington, VA
2 Baumgard, L. H., J. K. Sangster and D. E. Bauman. 2001. Milk fat synthesis in dairy cows is progressively reduced by increasing supplemental amounts of trans-10,cis-12 conjugated linoleic acid (CLA). J. Nutr. 131:1764-1769
3 Giesy, J. G., M. A. McGuire, B. Shafii and T. W. Hanson. 2002. Effect of dose of calcium salts of conjugated linoleic acid (CLA) on percentage and fatty acid content of milk fat in midlactation Holstein cows. J. Dairy Sci. 85:2023-2029   DOI   ScienceOn
4 Hanson, T. W., M. A. McGuire, J. M. Griinari, A. Sæbo, A. Vinci, and K. Cummings. 1998. Feeding of rumen protected conjugated linoleic acid (CLA) to lactating dairy cows results in increased CLA concentrations and milk fat depression. In:Proc. Pacific Northwest Anim. Nutr. Conf., Vancouver, British Columbia
5 Loor, J. J. and J. H. Herbein. 2001. Alterations in plasma and milk fatty acid profiles of lactating Holstein cows in response to ruminal infusion of a conjugated linoleic acid mixture. Anim. Res. 50:463-476   DOI   ScienceOn
6 Mackle, T. R., J. K. Kay, M. J. Auldist, A. K. H. McGibbon, B. A. Philpott, L. H. Baumgard and D. E. Bauman. 2003. Effects of abomasal infusion of conjugated linoleic acid on milk fat concentration and yield from pasture-fed dairy cows. J. Dairy Sci. 86:644-652   DOI   ScienceOn
7 Perfield, J. W., II, G. Bernal-Santos, T. R. Overton and D. E. Bauman. 2002. Effects of dietary supplementation of rumenprotected conjugated linoleic acid in dairy cows during established lactation. J. Dairy Sci. 85:2609-2617   DOI   ScienceOn
8 Piperova, L. S., U. Moallem, B. B. Teter, J. Sampugna, M. P. Yurawecz, K. M. Morehouse, D. Luchini and R. A. Erdman. 2004. Changes in milk fat in response to dietary supplementation with calcium salts of trans-18:1 or conjugated linoleic fatty acids in lactating dairy cows. J. Dairy Sci. 87:3836-3844   DOI   ScienceOn
9 Wang, J. H., S. H. Choi, C. G. Yan and M. K. Song. 2005. Effect of monensin and fish oil supplementation on biohydrogenation and CLA production by rumen bacteria in vitro when incubated with safflower oil. Asian-Aust. J. Anim. Sci. 18:221-225
10 Medeiros, S. R., D. E. Oliveira, L. J. Aroeira, M. A. McGuire and D. P. Lanna. 2000. The effect of long-term supplementation of conjugated linoleic acid (CLA) to dairy cows grazing tropical pastures. J. Dairy Sci. 83:169(Abstr.)
11 Loor, J. J., J. H. Herbein and C. E. Polan. 2002. Trans18:1 and 18:2 isomers in plasma and milk fat of grazing cows fed a grain supplement containing solvent-extracted or mechanically extracted soybean meal. J. Dairy Sci. 85:1197-1207   DOI   ScienceOn
12 Wang, J. H., S. H. Choi, K. W. Lim, K. H. Kim and M. K. Song. 2006. Effect of the mixed oil and monensin supplementation, and feeding duration of supplements on c9,t11-CLA contents in plasma and fat tissues of Korean Native (Hanwoo) steers. Asian-Aust. J. Anim. Sci. 19:1464-1469
13 Chouinard, P. Y., L. Corneau, A. S$\ae$b$\phi$ and D. E. Bauman. 1999a. Milk yield and composition during abomasal infusion of conjugated linoleic acids in dairy cows. J. Dairy Sci. 82:2737-2745
14 SAS. 1996. User's Guide: Statistics, Version 8 Edition. SAS Inst., Inc., Cary, NC
15 Goering, H. K. and P. J. Van Soest. 1970. Forage Fiber Analyses(Apparatus, Reagents, Procedures, and Some Applications). Agricultural Handbook No. 379. ARS-USDA, Washington, D.C
16 AbuGhazaleh, A. A., D. J. Schingoethe, A. R. Hippen and K. F. Kalscheur. 2003. Conjugated linoleic acid and vaccenic acid in rumen, plasma, and milk of cows fed fish oil and fats differing in saturation of 18 carbon fatty acids. J. Dairy Sci. 86:3648-3660   DOI   ScienceOn
17 Perfield, J. W., II, A. L. Lock, A. M. Pfeiffer and D. E. Bauman. 2004. Effects of amide-protected and lipid-encapsulated conjugated linoleic acid (CLA) supplements on milk fat synthesis. J. Dairy Sci. 87:3010-3016   DOI   ScienceOn
18 Dhiman, T. R., G. R. Anand, L. D. Satter and M. W. Pariza. 1999. Conjugated linoleic acid content of milk from cows fed different diets. J. Dairy Sci. 82:2146-2156   DOI   ScienceOn
19 Chin, S. F., W. Liu, J. M. Storkson, Y. L. Ha and M. W. Pariza. 1992. Dietary sources of conjugated dienoic isomers of linoleic acid a newly recognized class of anticarcinogens. J. Food Comp. Anal. 5:185-197   DOI
20 Baumgard, L. H., B. A. Corl, D. A. Dwyer, A. Sæbo and D. E. Bauman. 2000. Identification of the conjugated linoleic acid isomer that inhibits milk fat synthesis. Am. J. Physiol. 278:179-184
21 Noble, R. C., J. C. O'Kelly and J. H. Moore. 1972. Observations on the lecithin: cholesterol acyltransferase in bovine plasma. Biochim. Biophys. Acta 270:519-528   DOI   PUBMED   ScienceOn
22 Baumgard, L. H., E. Matitashvili, B. A. Corl, D. A. Dwyerand and D. E. Bauman. 2002. Trans-10,cis-12 conjugated linoleic acid decreases lipogenic rates and expression of genes involved in milk lipid synthesis in dairy cows. J. Dairy Sci. 85:2155-2163   DOI   ScienceOn
23 Loor, J. J. and J. H. Herbein. 2003. Reduced fatty acid synthesis and desaturation due to exogenous trans 10,cis 12-CLA in cows fed oleic or linoleic oil. J. Dairy Sci. 86:1354-1369   DOI   ScienceOn
24 Bernal-Santos, G., J. W. Perfield II, D. M. Barbano, D. E. Bauman and T. R. Overton. 2003. Production responses of dairy cows to dietary supplementation with conjugated linoleic acid (CLA) during the transition period and early lactation. J. Dairy Sci. 86:3218-3228   DOI   ScienceOn
25 Chouinard, P. Y., L. Corneau, D. M. Barbano, L. E. Metzger and D. E. Bauman. 1999b. Conjugated linoleic acids alter milk fatty acid composition and inhibit milk fat secretion in dairy cows. J. Nutr. 129:1579-1584   DOI   ScienceOn
26 Loor, J. J. and J. H. Herbein. 1998. Exogenous conjugated linoleic acid isomers reduce bovine milk fat concentration and yield by inhibiting de novo fatty acid synthesis. J. Nutr. 128:2411-2419