• TTA Journal
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• s.61
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• pp.128-133
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• 1999

우정성은 통신백서(1998년 통신에 관한 현황 보고)를 5월 26일 각료 회의 후에 공표하였다. 이번 백서는 $\ulcorner$디지털 네트워크 사회 개막$\~$변해가는 라이프스타일$\~$$\lrcorner$이라는 제목의 특집호로 구성되었다. 본 특집에서는 급속히 보급되고 있는 인터넷, 휴대전화, CS 디지털 방송 등의 미디어가 일, 취미$\cdot$오락, 학습 등의 생활에 주는 영향을 분석하고 있다. 또 정보 literacy의 일$\cdot$미 비교 등도 실시하여 $\ulcorner$다양한 선택$\lrcorner$과 $\ulcorner$자유로운 참가$\lrcorner$의 기회가 제공되어,$\ulcorner$개성 발휘$\lrcorner$를 할 수 있는 보다 풍부하게 열린 $\ulcorner$디지털 네트워크 사회$\lrcorner$실현을 위한 과제를 제시하고 있다. 이외에 본 백서에서는 정보통신의 현황분석이나, 정보통신산업의 일$\cdot$미 비교, 사이버 비즈니스의 특성 분석, 도도부현의 정보화 분석 등을 나타내고 있다. 또한 작년에 이어, CD-ROM판을 첨부한 책자 및 DVD-ROM판 백서를 판매하고 있다. 금년부터 일본의 백서로는 처음으로, 인터넷상에 공표일 당일부터 백서 전문을 게재하였다. 1998년 백서는 3장으로 구성되어 있는데 각 장의 포인트를 소개하고자 한다.

• Nuclear Engineering and Technology
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• v.55 no.7
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• pp.2366-2372
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• 2023

Are borate and silicate glasses suitable for working as shieling materials against fast neutrons? To correctly answer the above question, some silicate, and borate-based glasses were fabricated and irradiated with various doses of fast neutrons varied between 1.73 and 12.10 MGy. The color and hardness of the fabricated glasses were affected by the fast neutron fluence where the transparent glasses turned colored as well as the hardness of the fabricated glasses was decreased. The gamma-ray spectrometric analysis shows a high activity concentration produced in the barium borate glasses due to the formation of radioisotopes Ba-131 and Ba-133 reaches to 5.92E+05 Bq and 4.25E+03 Bq, respectively for sample Cd-5 Batch 3. Additionally, the gamma-ray spectrometric analysis for the sodium silicate glasses shows low activity concentrations emitted from isotopes formed due to the activation of Y₂O₃-associated impurities. These activities are low compared to that emitted by barium borate-based glasses.

• Journal of Genetic Medicine
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• v.4 no.2
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• pp.133-141
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• 2007

Purpose : Cri-du-Chat syndrome (CdCs) is a rare but clinically recongnizable condition with an estimated incidence of 1:50,000 live births. The clinical characteristics of the syndrome include severe psychomotor and mental retardation, microcephaly, hypertelorism, hypotonia, and slow growth. Also the size of the chromosome 5p deletion ranges were known from the region 5p13 to the terminal region. In this study, we report the spectrum of 5p deletion in Korean 20 pts. with CdCs and genotype-phenotype associations in CdCs. Methods : In order to delineate genotype-phenotype correlation, molecular cytogenetic studies including GTG banding and clinical characterization were performed on Korean 20 pts with CdCs including parents. CGH array and Fluorescence in situ hybridization (FISH) analysis were used to confirm a terminal deletion karyotype and map more precisely the location of the deletion breakpoint. Results : Molecular analysis of the spectrum of 5p deletion revealed 9 pts (45%) with a del (5)(p14), 7 pts. (35%) a del (5)(p13), 3 pts. (15%) a del (5)(p15.1) and 1 pt. (5%) a del (5)(p15.2) in 20 pts with CdCs. 4(20%)pts were identified to have additional chromosome abnormalites of deficiency and duplication involving chromosomes of 6, 8, 18, & 22. Parental study identified 3 familial case (2 paternal and 1 maternal origin) showing parents being a balanced translocation carrier. And the comparison study of the deletion break points among these 20 pts. with their phenotype has showed the varying clinical pheno-types in the CdCs critical region. Conclusion : The characterization of 5p deletion including parental study may help to delineate the genotypephenotype correlation in CdCs. Also these molecular cytogenetic analyses will be able to offer better information for accurate genetic diagnosis in CdCs and further make possible useful genetic counseling in pts. and family.

• Journal of Veterinary Clinics
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• v.26 no.6
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• pp.528-533
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• 2009

In the current study, the mesenchymal stem cells (MSCs) isolated and propagated from the human umbilical cord blood (UCB) were tested for their capabilities of differentiation into chondrocytes in vitro. The mesenchymal progenitor cells (MPCs) collected from UCB were cultured in a low glucose DMEM medium with 10% FBS, L-glutamine and antibiotics. The human MSC colonies were positively stained by PAS reaction. When the immunophenotypes of surface antigens on the MSCs were analyzed by fluorescence-activated cell sorter (FACS) analysis, these cells expressed positively MSC-related antigens of CD 29, CD44, CD 90 and CD105, whereas they did not express antigens of CD14, CD31, CD34, CD45, CD133 and HLA-DR. Following induction these MSCs into chondrocytes in the chondrogenic differentiation medium for 3 weeks or more, the cells were stained positively with safranin O. We clearly confirmed that human MSCs were successfully differentiated into chondrocytes by RT-PCR and immunofluorescent stain of type-II collagen protein. These data also indicate that the isolation, proliferation and differentiation of the hUCB-derived MSCs in vitro can be used for elucidating the mechanisms involved in chondrogenesis. Moreover this differentiation technique can be applied to developing cell-based tissue regeneration or repair damaged tissues.

• Development and Reproduction
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• v.22 no.4
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• pp.379-391
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• 2018

Through the development of organic synthetic skill, chemicals that mimic signaling mediators such as steroid hormones have been exposed to the environment. Recently, it has become apparent that this circumstance should be further studied in the field of physiology. Estrogenic action of chronic low-dose nonylphenol (NP) and di-(2-ethylhexyl) phthalate (DEHP) in mouse uterus was assessed in this study. Ten to twelve-week-old female mice (CD-1) were fed drinking water containing NP (50 or $500{\mu}g/L$) or DEHP (133 or $1,330{\mu}g/L$) for 10 weeks. Uterine diameter, the thickness of myometrium and endometrium, and the height of luminal epithelial cells were measured and the number of glands were counted. The expression levels of the known $17{\beta}$-estradiol ($E_2$)-regulated genes were evaluated with real-time RT-PCR methodology. The ration of uterine weight to body weight increased in $133{\mu}g/L$ DEHP. Endometrial and myometrial thickness increased in 133 and $1,330{\mu}g/L$ DEHP treated groups, and in 50, $500{\mu}g/L$ NP and $133{\mu}g/L$ DEHP, respectively. The height of luminal epithelial cell decreased in NP groups. The numbers of luminal epithelial gland were decreased in NP groups but increased in $50{\mu}g/L$ DEHP group. The histological characters of glands were not different between groups. The mRNA expression profiles of the known $17{\beta}$-estradiol ($E_2$) downstream genes, Esr1, Esr2, Pgr, Lox, and Muc1, were also different between NP and DEHP groups. The expression levels dramatically increased in some genes by the NP or DEHP. Based on these results, it is suggested that the chronic low-dose NP or DEHP works as estrogen-like messengers in uterus with their own specific gene expression-regulation patterns.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.2
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• pp.437-442
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• 2015

Aim: To establish a pancreatic cancer stem cell model using human pancreatic cancer cells in nude mice to provide a platform for pancreatic cancer stem cell research. Materials and Methods: To establish pancreatic cancer xenografts using human pancreatic cancer cell line SW1990, nude mice were randomly divided into control and gemcitabine groups. When the tumor grew to a volume of $125mm^3$, they treated with gemcitabine at a dose of 50mg/kg by intraperitoneal injection of 0.2ml in the gemcitabine group, while the mice in control group were treated with the same volume of normal saline. Gemcitabine was given 2 times a week for 3 times. When the model was established, the proliferation of pancreatic cancer stem cells was observed by clone formation assay, and the protein and/or mRNA expression of pancreatic stem cell surface markers including CD24, CD44, CD133, ALDH, transcription factors containing Oct-4, Sox-2, Nanog and Gli, the key nuclear transcription factor in Sonic Hedgehog signaling pathway was detected by Western blot and/or RT-PCR to verify the reliability of this model. Results: This model is feasible and safe. During the establishment, no mice died and the weight of nude mice maintained above 16.5g. The clone forming ability in gemcitabine group was stronger than that of the control group (p<0.01). In gemcitabine group, the protein expression of pancreatic cancer stem cell surface markers including CD44, and ALDH was up-regulated, the protein and mRNA expression of nuclear transcription factor including Oct-4, Sox-2 and Nanog was also significantly increased (P<0.01). In addition, the protein expression of key nuclear transcription factor in Sonic Hedgehog signaling pathway, Gli-1, was significantly enhanced (p<0.01). Conclusions: The pancreatic cancer stem cell model was successfully established using human pancreatic cancer cell line SW1990 in nude mice. Gemcitabine could enrich pancreatic cancer stem cells, simultaneously accompanied by the activation of Sonic Hedgehog signaling pathway.

• The Journal of Dong Guk Oriental Medicine
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• v.7 no.1
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• pp.119-133
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• 1998

This study was performed to investigated the effects of an immunosuppression and a mitigation of inflammation of Scutellaria baicalensis GEORGI on Allergic contact dermatitis. In order to study, after oral administration of Scutellaria baicalensis GEORGI extract, contact hypersensitivity assay, general morphologic change of skin, sulfated acid mucosubstance, mast cell, IL-2R, ICAM-1 and CD11b were observed in BALB/C mouse induced allergic contact dermatitis by the contact sensitizing DNCB. Ear swelling in contact hypersensitivity assay was decreased in the Scutellaria baicalensis GEORGI extracts administered(HEGT) group, compared with DNCB painted group. In the general morphologic change of skin, hyperplasia of keratinocytes, increase of lymphocytes and inflammatory cell, increase of vasculogenesis and epidermal lymphocytes infiltration, increase of cell damage in epidermal basal layer and prickle layer were decreased in the HEGT group, compared with DNCB painted group. Increase of sulfated acid mucosubstance, mast cell, IL-2R positive cell, ICAM-1 positive cell, CD11b positive cell were decreased in the HEGT group, compared with DNCB painted group.

• Microbiology and Biotechnology Letters
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• v.40 no.2
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• pp.117-127
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• 2012

The beneficial effects of adipose-derived stem cell conditioned media (ADSC-CM) for skin regeneration have previously been reported, despite the precise mechanism of how ADSC-CM promotes skin regeneration remaining unclear. ADSC-CM contains various secretomes and this may be a factor in it being a good resource for the treatment of skin conditions. It is also known that ADSC-CM produced in hypoxia conditions, in other words Advanced Adipose-Derived Stem cell Protein Extract (AAPE), has excellent skin regenerative properties. In this study, a human primary skin cell was devised to examine how AAPE affects human dermal fibroblast (HDF) and human keratinocyte (HK), which both play fundamental roles in skin regeneration. The promotion of collagen formation by HDFs was observed at 0.32 mg/ml of AAPE. AAPE treatment significantly stimulated stress fiber formation. DNA gene chips demonstrated that AAPE in HKs (p<0.05) affected the expression of 133 identifiable transcripts, which were associated with cell proliferation, migration, cell adhesion, and response to wounding. Twenty five identified proteins, including MMP, growth factor and cytokines such as CD54, FGF-2, GM-CSF, IL-4, IL-6, VEGF, TGF-${\beta}2$, TGF-${\beta}3$, MMP-1, MMP-10, and MMP-19, were contained in AAPE via antibody arrays. Thus, AAPE might activate the HK biological function and induce the collagen synthesis of HDF. These results demonstrate that AAPE has the potential to be used for clinic applications aimed at skin regeneration.

• Nuclear Engineering and Technology
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• v.4 no.4
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• pp.340-344
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• 1972

Isomeric ratios were measured for the capture of thermal neutron by $^{79}$ Br, $^{80}$ Se, $^{103}$ Rh, $^{115}$ In and $^{133}$ Cs as well as those of epi-cadmium neutron by $^{79}$ Br, $^{80}$ Se and $^{l33}$Cs. The measurements were performed by analysing decay curves obtained by ${\gamma}$-ray spectrometry after irradiation. The counting efficiency curve was determined by using the calibrated standard sources with overall uncertainties of about 1%. Isomeric ratios, given in $\sigma$ high spin/($\sigma$ high spin + $\sigma$ low spin), of $^{80, 80m}$Br, $^{81,81m}$Se, $^{014, 104m}$Rh, $^{116,116m}$In and $^{134, 134m}$Cs produced by thermal neutron activation were found to be 0.21$\pm$0.01, 0.14$\pm$0.02, 0.12$\pm$0.02, 0.69$\pm$0.07 and 0.058$\pm$0.004, respectively, Those values of $^{80, 80m}$Br, $^{81,81m}$Se, and $^{134, 134m}$Cs Produced by epi-cadmium neutron were found to be 0.19$\pm$0.02, 0.29$\pm$0.02 and 0.074$\pm$0.011, respectively. The experimental values obtained were compared with the theoretical values deduced from the statistical model. There were the general agreements between the theory and the experiment.t.

• Korean Journal of Food Science and Technology
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• v.54 no.1
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• pp.28-34
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• 2022

Glioblastoma is the most common primary malignant brain tumor and has an extremely poor prognosis. Glioblastoma stem cells (GSCs) contribute to tumor initiation, recurrence, and resistance to therapy, and are thus a key therapeutic target. The peel of Citrus unshiu Markovich has been used in traditional medicine in East Asia to treat various diseases. In this study, we investigated the anticancer activity and molecular mechanism of the chloroform extract of this natural product (CECU) in U87MG GSCs. The results show that CECU inhibited the proliferation, tumorsphere formation, and migration of U87MG GSCs by causing cell cycle arrest at the G0/G1 phase and apoptosis. In addition, CECU downregulated key cancer stemness regulators, including CD133, Oct4, Nanog, integrin α6, ALDH1A1, and STAT3 signaling in U87MG GSCs. Furthermore, CECU significantly suppressed in vivo tumor growth of U87MG GSCs in a chorioallantoic membrane model. Therefore, CECU can be utilized as a natural medicine for the prevention and treatment of glioblastoma.