Browse > Article

Chondrogenesis of Mesenchymal Stem Cells Derived from Human Umbilical Cord Blood  

Koh, Phil-Ok (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
Cho, Jae-Hyun (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
Nho, Kyoung-Hwan (Laboratory of Stem cell and Tumor Biology, Department of Veterinary Public Health, and Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University)
Cha, Yun-Im (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
Kim, Young-Ki (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
Cho, Eun-Hae (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
Lee, Hee-Chun (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
Jung, Tae-Sung (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
Yeon, Seong-Chan (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
Kang, Kyung-Sun (Laboratory of Stem cell and Tumor Biology, Department of Veterinary Public Health, and Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University)
Lee, Hyo-Jong (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
Publication Information
Journal of Veterinary Clinics / v.26, no.6, 2009 , pp. 528-533 More about this Journal
Abstract
In the current study, the mesenchymal stem cells (MSCs) isolated and propagated from the human umbilical cord blood (UCB) were tested for their capabilities of differentiation into chondrocytes in vitro. The mesenchymal progenitor cells (MPCs) collected from UCB were cultured in a low glucose DMEM medium with 10% FBS, L-glutamine and antibiotics. The human MSC colonies were positively stained by PAS reaction. When the immunophenotypes of surface antigens on the MSCs were analyzed by fluorescence-activated cell sorter (FACS) analysis, these cells expressed positively MSC-related antigens of CD 29, CD44, CD 90 and CD105, whereas they did not express antigens of CD14, CD31, CD34, CD45, CD133 and HLA-DR. Following induction these MSCs into chondrocytes in the chondrogenic differentiation medium for 3 weeks or more, the cells were stained positively with safranin O. We clearly confirmed that human MSCs were successfully differentiated into chondrocytes by RT-PCR and immunofluorescent stain of type-II collagen protein. These data also indicate that the isolation, proliferation and differentiation of the hUCB-derived MSCs in vitro can be used for elucidating the mechanisms involved in chondrogenesis. Moreover this differentiation technique can be applied to developing cell-based tissue regeneration or repair damaged tissues.
Keywords
mesenchymal stem cells; human umbilical cord blood; chondrogenesis; FACs analysis; RT-PCR; Safranin-O staining; immunocytochemical staining;
Citations & Related Records

Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 Deans RJ, Moseley AB. Mesenchymal stem cells: biology and potential clinical uses. Exp Hematol 2000; 28: 875-884   DOI   ScienceOn
2 Krampera M, Pizzolo G, Aprili G, Franchini M. Mesenchymal stem cells for bone, cartilage, tendon and skeletal muscle repair. Bone 2006; 39: 678-683   DOI   ScienceOn
3 Ringe J, Kaps C, Burmester GR, Sittinger M. Stem cells for regenerative medicine: advances in the engineering of tissues and organs. Naturwissenschaften 2002; 89: 338-351   DOI   ScienceOn
4 Yang SE, C.W. Ha, M. Jung, H.J. Jin, H. Song and S. Choi, et al., Mesenchymal stem/progenitor cells developed in cultures from UC blood, Cytotherapy 6 (2004), pp. 476-486   DOI   ScienceOn
5 Zeng L, Rahrmann E, Hu Q, Lund T, Sandquist L, Felten M, O'Brien TD, Zhang J, Verfaillie C. Multipotent adult progenitor cells from swine bone marrow. Stem Cells 2006; 24: 2355-2366   DOI   ScienceOn
6 Oh W, Kim DS, Yang YS and Lee JK. Immunological properties of umbilical cord blood-derived mesenchymal stromal cells. Cellular Immunology. 2008; 251: 116-123   DOI   ScienceOn
7 Barry FP, Murphy JM. Mesenchymal stem cells: clinical applications and biological characterization. Int J Biochem Cell Biol 2004; 36: 568-584   DOI   ScienceOn
8 Lee KD, Kuo TK, Whang-Peng J, Chung YF, Lin CT, Chou SH, Chen JR, Chen YP, Lee OK. In vitro hepatic differentiation of human mesenchymal stem cells. Hepatology 2004; 40: 1275-1284   DOI   ScienceOn
9 Prockop DJ. Marrow stromal cells as stem cells for nonhematopoietc tissues. Science 1997; 276: 71-74   DOI
10 In't Anker PS, Scherjon SA, Kleijburg-van der Keur C, de Groot-Swings GM, Claas FH and Fibbe WE, et al., Isolation of mesenchymal stem cells of fetal and maternal origin from human placenta. Stem Cells 2004; 22: 1338-1345   DOI
11 In't Anker PS, Noort W.A, Scherjon SA, Kleijburg-van der Keur C, Kruisselbrink AB and van Bezooijen RL, et. al., Mesenchymal stem cells in human second-trimester bone marrow, liver, lung, and spleen exhibit a similar immunophenotype but a heterogeneous multilineage differentiation potential, Haematologica 2003; 88: 845–852
12 Jiang Y, Jahagirda BN, Reinhardt RL, Schwartz RE, Keene CD, Oritz-Gonzalez XR, et al. Pluripotency of mesenchymal stem cells derived from adult marrow. Nature 2002; 418: 41-49   DOI   ScienceOn
13 Colleoni S, Donofrio G, Lagutina I, Duchi R, Galli C, Lazzari G. Establishment, differentiation, electroporation, viral transduction, and nuclear transfer of bovine and porcine mesenchymal stem cells. Cloning Stem Cells 2005; 7: 154-166   DOI   ScienceOn
14 Faast R, Harrison SJ, Beebe LF, McIlfatrick SM, Ashman RJ, Nottle MB. Use of adult mesenchymal stem cells isolated from bone marrow and blood for somatic cell nuclear transfer in pigs. Cloning Stem Cells 2006; 8: 166-173   DOI   ScienceOn
15 Lee, O.K, Kuo TK, Chen WM, Lee KD, Hsieh SL and Chen TH. Isolation of multipotent mesenchymal stem cells from umbilical cord blood, Blood. 2004;103: 1669-1675   DOI   ScienceOn
16 Jiang Y, Vaessen B, Lenvik T, Blackstad M, Reyes M, Verfaillie CM. Multipotent progenitor cells can be isolated from postnatal murine bone marrow, muscle, and brain. Exp Hematol 2002; 30: 896-904   DOI   ScienceOn
17 Krampera M, Passini A, Rigo A, Scupoli MT, Tecchio C, Malpeli G, et al. HB-EGF/HER-1 signaling in bone marrow mesenchymal stem cells inducing cell expansion and reversibly preventing multi-lineage differentiation. Blood 2005; 106: 59-66   DOI   ScienceOn
18 Bosch P, Pratt SL, Stice SL. Isolation, characterization, gene modification, and nuclear reprogramming of porcine mesenchymal stem cells. Biol Reprod 2006; 74: 46-57   DOI   ScienceOn
19 Minguell JJ, Erices A, Conget P. Mesenchymal stem cells. Exp Biol Med (Maywood). 2001; 226: 507-520
20 Wang M, Yang Y, Yang D, Luo F, Liang W, Guo S and Xu J. The immunomodulatory activity of human umbilical cord blood-derived mesenchymal stem cells in vitro. Immunology 2008; 126: 220-223   DOI   ScienceOn
21 Lee RH, Kim B, Choi I, Kim H, Choi HS, Suh K, Bae YC, Jung JS. Characterization and expression analysis of mesenchymal stem cells from human bone marrow and adipose tissue. Cell Physiol Biochem 2004; 14: 311-324   DOI   ScienceOn
22 Erices A, Conget P and Minguel JJ. Mesenchymal progenitor cells in human umbilical cord blood, Br J Haematol 2000; 109: 235-242   DOI   ScienceOn