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The Role of Vitamin C and Vitamin E Supplementation on Iron Contents and Biomarkers of Oxidative Stress in Blood, Liver and Brain of Aging Rats (나이에 따른 흰쥐의 혈액, 간, 뇌조직의 철분함량, 산화 스트레스 지표에 대한 비타민 C 와 비타민 E공급의 역할)

  • 황은희
    • Journal of Nutrition and Health
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    • v.33 no.5
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    • pp.507-516
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    • 2000
  • The purpose of this study was to investigate the effect of vitamin C and vitamin E supplementation on the iron contents and oxidative stress of the rats. Rats were fed 18g ascorbic acid and 300IU $\alpha$-tocopherol/kg diet, respectively. Rats were sacrificed at 1, 3, 5 and 7 month of age. The blood, liver and brain were selected for the quantitation of iron and malondialdehyde(MDA) contents, glutathione peroxidase(GSHPx), superoxided dismutase(SOD) and catalase(CAT) activity. Iron and MDA contents and GSHPx activities were increased with aging. Vitamin C and Vitamin E supplementation increased iron contents of the plasma. Vitamin C raised iron contents, but vitamin E decreased iron contents of the liver. In the brain vitamin C and vitamin E did not affect the iron level. MDA levels were decreased with vitamin C and vitamin E supplementation in the erythrocyte and liver, and vitamin C supplementation elevated MDA levels in the brain. GSHPx activity was increased with vitamin C and vitamin E supplementation. SOD activities of erythroucyte and brain were not affected with age, but in the liver, SOD activity was raised with age and vitamin C supplementation. Vitamin C and vitamin E supplementation promoted CAT activity of erythroucyte and liver, and CAT activity of brain was eleveated with vitamin addition but was decreaed with vitamin E addition. Vitamin C and vitamin E decreased iron contents of blood plasma, MDA contents of plasma and liver, and CAT activity of erythrocyte. Above results indicated that iron contents and biomarkers of oxidative stress were more affected by age than antioxidant action of vitamin C and vitamin E.

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Simultaneous Removal of Organic Pollutants, N, P, and Antibiotics from Liquid Fertilizer using a Microbubble and Catalyst Coupling System (마이크로버블/촉매 융합 시스템을 이용한 액비 내 유기오염물질, N, P 및 항생제 동시 제거)

  • Lee, Dong Gwan;Sim, Young Ho;Paek, Yee;Kwon, Jin Kyung;Jang, Jae Kyung
    • Journal of Environmental Science International
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    • v.28 no.11
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    • pp.983-991
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    • 2019
  • This study investigated the use of a hydroxyl-radicals-generated microbubble/catalyst (MB/Cat) system for removing organic pollutants, nitrogen, and phosphorous from liquid fertilizer produced by livestock wastewater treatment. Use of the MB/Cat system aims to improve the quality of liquid fertilizer by removing pollutants originally found in the wastewater. In addition, a reduction effect has been reported for antibiotics classified as representative non-biodegradable matter. Samples of liquid fertilizer produced by an aerobic biological reactor for swine wastewater treatment were first analyzed for initial concentrations of pollutants and antibiotics. When the MB/Cat system was applied to the liquid fertilizer, TCOD, TOC, $BOD_5$, and $NH_3-N$, and $PO_4-P$ removal efficiencies were found to be approximately 52%, 51%, 30%, 21%, and 66%, respectively. Additionally, Amoxicillin hydrate was removed by 10%, and Chlortetracycline HCl and Florfenicol were not present at detectable levels These findings confirm that the MB/Cat system can be used with livestock wastewater treatment to improve liquid fertilizer quality and to process wastewater that is safe for agricultural re-use.

A Development Study on New Hand Rehabilitation Training Tool Using Cat's Cradle Game (실뜨기 놀이를 활용한 새로운 수부재활훈련도구 개발 연구)

  • Lee, Yu Sol;Chung, Do Sung
    • Design Convergence Study
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    • v.17 no.3
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    • pp.1-19
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    • 2018
  • Hand rehabilitation training tools are used in hospitals and at home for patients and users who require recovery of disabled hands and improvement in overall hand function. However, existing training tools are not organized into a progressive system, and they lead to repeatability operations over a period of time. As a result, patients feel free and cannot be motivated by rehabilitation, and continuous rehabilitation training is difficult. Based on this argument, the study combines one of the elements of the game called the "Cat's cradle" to enable the user to feel achievement through play and to achieve natural rehabilitation through unconsciousness. After examining the characteristics of the tool, the user's environment, the relevance of the Cat's cradle game to the training tool and to the patient's continued rehabilitation was established. And design elements were derived through professional interviews. Later, design guidelines and prototypes have been created to complement the problems associated with guidelines and prototypes by conducting usability testing and design element assessment.

Change in Polyphenol Content, Antioxidant Activity, and Antioxidant Enzyme Status of Cowpea During Germination (동부 발아기간 중 폴리페놀 함량, 항산화성 및 항산화효소 활성 변이)

  • Chon, Sang-Uk
    • Korean Journal of Plant Resources
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    • v.26 no.1
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    • pp.60-67
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    • 2013
  • A laboratory experiment was conducted to determine the content of phenolics and flavonoids, antioxidant activity and antioxidant enzyme activity for the extracts from cowpea seed and sprouts. Plant length and weight of cowpea sprouts were significantly increased until 7 days after seeding. Total phenolics level [mg chlorogenic acid equivalents (CAE) $kg^{-1}$ DW] was highest in dry seed (DS) extracts of cowpea ($63.9mg\;kg^{-1}$), followed by imbibed seed (IS) ($56.8mg\;kg^{-1}$) and 1-day-old sprout (1DOS) extracts ($46.4mg\;kg^{-1}$), and significantly reduced with increase of sprout age (p < 0.05). The antioxidant activity of the methanol extracts from all the samples showed same tendency to the results of total phenolics level, and dose-dependently increased. DPPH (1,1-diphenyl-2-picryl hydrazyl radical) free radical scavenging activity was higher in DS (87.3%) and IS (41.2%) than in cowpea sprouts from 1DOS to 7DOS, ranging from 17.1 to 30.4%. Antioxidant enzymes, APX, POX, and POX activities were highest in 7DOS and lowest in DS. SOD activity showed much higher activity in sprouts and in seeds. Correlation coefficient between physiological-active substance and the activity was highest between APX and CAT activities ($r^2$= 0.9574). Especially, total phenolics content was more highly correlated with antioxidant or with antioxidant enzyme activities than was total flavonoid level.

The effects of different metal posts, cements, and exposure parameters on cone-beam computed tomography artifacts

  • Ana Priscila Lira de Farias Freitas;Larissa Rangel Peixoto;Fernanda Clotilde Mariz Suassuna;Patricia Meira Bento;Ana Marly Araujo Maia Amorim;Karla Rovaris Silva;Renata Quirino de Almeida Barros;Andrea dos Anjos Pontual de Andrade Lima;Daniela Pita de Melo
    • Imaging Science in Dentistry
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    • v.53 no.2
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    • pp.127-135
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    • 2023
  • Purpose: This study assessed the intensity of artifacts produced by 2 metal posts, 2 cements, and different exposure parameters using 2 cone-beam computed tomography (CBCT) units. Materials and Methods: The sample was composed of 20 single-rooted premolars, divided into 4 groups: Ni-Cr/zinc phosphate, Ni-Cr/resin cement, Ag-Pd/zinc phosphate, and Ag-Pd/resin cement. Samples were scanned before and after post insertion and cementation using a CS9000 3D scanner with 4 exposure parameters (85/90 kV and 6.3/10 mA) and an i-CAT scanner with 120 kV and 5 mA. The presence of artifacts was assessed subjectively by 2 observers and objectively by a trained observer using ImageJ software. The Mann-Whitney, Wilcoxon, weighted kappa, and chi-square tests were used to assess data at a 95% confidence level(α<0.05). Results: In the subjective analyses, AgPd presented more hypodense and hyperdense lines than NiCr (P<0.05), and more hypodense halos were found using i-CAT (P<0.05) than using CS9000 3D. More hypodense halos, hypodense lines, and hyperdense lines were observed at 10 mA than at 6.3 mA (P<0.05). More hypodense halos were observed at 85 kV than at 90 kV (P<0.05). CS9000 3D presented more hypodense and hyperdense lines than i-CAT (P<0.05). In the objective analyses, AgPd presented higher percentages of hyperdense and hypodense artifacts than NiCr (P<0.05). Zinc phosphate cement presented higher hyperdense artifact percentages on CS9000 3D scans(P<0.05). CS9000 3D presented higher artifact percentages than i-CAT(P<0.05). Conclusion: High-atomic-number alloys, higher tube current, and lower tube voltage may increase the artifacts present in CBCT images.

The evaluation of applicability for agricultural reservoir of CAT(Catchment hydrologic cycle Assessment Tool) (CAT 모형의 농업용 저수지 유역에 대한 적용성 평가)

  • Jang, Cheol-Hee;Kim, Hyeon-Jun
    • Proceedings of the Korea Water Resources Association Conference
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    • 2011.05a
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    • pp.121-121
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    • 2011
  • 도시유역 물순환 해석 모형(Catchment hydrologic cycle Analysis Tool, CAT)은 기존의 개념적 매개변수 기반의 집중형 수문모형과 물리적 매개변수 기반의 분포형 수문모형의 장점을 최대한 집약하여, 도시유역 개발 전/후의 장/단기적 물순환 변화특성을 정량적으로 평가하고 물순환 개선시설의 효과적인 설계를 지원하기 위한 물순환 해석 모형이다. 이 모형은 수문학적으로 균일하게 판단되는 범위를 소유역으로 분할하여 지형학적 요인에 의한 유출 특성을 객관적으로 반영할 수 있으며, 개발 공간 단위별로 침투, 증발, 지하수 흐름 등의 모의가 가능하도록 하는 링크-노드 방식으로 개발되었다. 모형의 UI(User Interface)는 사용자가 손쉽게 모형을 적용/관리하고, 여러 시나리오를 동시에 효과적으로 모의하여 분석할 수 있도록 설계되었다. 또한, 모든 입/출력 자료를 엑셀이나 텍스트 형식과 연동되도록 하여 프로젝트별 매개변수 관리가 용이하도록 개발하였다. 특히 본 모형에서는 사용자의 목적에 맞는 다양한 물순환 개선시설(침투시설, 저류지, 습지, 빗물저장시설, 리사이클 및 외부급수 등)의 구현 및 모의가 가능하도록 개발하였다. CAT은 수자원의지속적확보기술개발사업(2008 ~ 2011)의 연구 성과로서 한국건설기술연구원에서 개발하였다. 2008년 말에 모형의 기본구조가 개발되었고, 2009년에는 세부 알고리즘인 증발산, 침투, 유역 유출, 지하수 유거, 하도추적 등의 모듈과 사용자 편의시스템이 개발되었다. 2010년에는 우리나라 논의 특성을 반영한 논 유출 해석 모듈 및 저류지, 침투시설, 습지, 빗물이용시설 및 하천에서의 취수와 도수 등과 같은 물순환 개선시설을 평가할 수 있는 모듈을 추가하여 개발하였으며 2010년 3월에 도시유역 물순환 해석 모형 1.0 베타 버전을 출시하였다. 2010년 12월 에는 1.0 베타 버전에 침투해석모듈(Green&Ampt, Horton), 논에서의 개량물꼬 배수, 침투녹지(Bioretention) 및 차집관거 기능을 추가하였고, 기타 GUI의 업그레이드 및 추가를 통하여 1.5 베타 버전을 출시하였다. 현재까지 여러 자연유역과 신도시 개발지역에 대한 적용을 통하여 모형의 적용성을 평가하였다. 본 연구에서는 기존의 자연유역과 신도시 개발지역 외에 농업용 저수지와 논 관개지구가 위치한 유역을 대상으로 모형의 적용성을 평가하고자 하였다. 대상유역은 농업용수 지구이며 농업수리시설의 종류와 규모가 다양할 뿐만 아니라 농촌유역으로써의 대표성을 가지고 기존의 관측자료가 풍부한 점 등을 고려하여 경기도 평택의 이동유역을 선정하였다. 이동유역은 행정구역으로는 경기도 용인시 이동면, 남사면 일원이며 서쪽은 경기도 오산시, 남쪽은 평택시, 안성시 그리고, 북쪽은 용인시와 인접하고 있다. 이동유역 내 주요시설로서 유역면적 $94.4km^2$의 이동저수지와 상류에 용덕저수지($12.41km^2$)와 미산저수지($4.39km^2$), 노곡저수지($2.00km^2$)의 3개 저수지가 위치하며 2개의 유입하천(진위천, 송전천)에 의해 이동저수지로 유입된다.

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Magnetic Resonance Imaging Features of Suspected Acute Spinal Cord Infarction in Two Cats (두 마리 고양이에서 발생한 급성 척수 경색의 자기 공명 영상학적 진단 증례)

  • Jung, Sun-Young;Kim, Bo-Eun;Ji, Seo-Yeoun;Yoon, Jung-Hee;Choi, Min-Cheol
    • Journal of Veterinary Clinics
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    • v.30 no.4
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    • pp.320-323
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    • 2013
  • Spinal cord infarction is becoming recognized as an important cause of acute myelopathy in cats. Although the definitive diagnosis is confirmed through postmortem histopathologic examination, MR imaging features provide valuable informations for the diagnosis of spinal cord infarction. The aim of this report is to describe MR findings of acute spinal cord infarction in two cats and to evaluate usefulness of low field MRI (0.3Tesla) as a potential diagnostic tool of acute spinal cord infarction. A cat (unknown age, neutered male mixed breed cat) was referred one day after the acute onset of non-ambulatory spastic tetraparesis and the other cat (a 9-year-old, neutered female domestic short hair cat) was presented due to the acute onset of non-ambulatory paraparesis and one day later paraplegia. The lesions of the MR images were shown on the spinal cord parenchyma over C2 to C6 in case 1 and L2 to L5 in case 2. The MR images in these two cases were characterized by focal intramedullary lesions, mainly involving grey matter which were hyperintense T2 weighted and FLAIR images and hyperintense on DWI and hypointense on ADC map. The MR findings in both cases were highly suggestive of acute spinal cord infarctions, based upon previous reported small animal cases and human cases. In conclusion, based on MR features, together with the history and clinical examination findings, MRI modality can be used as an antemortem tool for the diagnosis of acute spinal cord infarction in cats.

Antioxidative Effects of Red Ginseng Saponins on Paraquat-induced Oxidative Stress (Paraquat 유도 산화적 스트레스에 대한 홍삼 사포닌의 항산화 효과)

  • Kim, Dong-Jo;Seong, Kum-Soo;Kim, Dong-Won;Kim, Seong-Ruyong;Chang, Che-Chul
    • Journal of Ginseng Research
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    • v.28 no.1
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    • pp.5-10
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    • 2004
  • This study was carried out to investigate the effect of the active ingredients from ginseng on paraquat(PQ) toxicity. Oxidative stress was induced by intraperitreatneal injection of PQ at a single dose of 25 mg/kg. Saponin treated groups were given protopanaxadiol saponins(PPD) or protopanaxatriol saponins(PPT)(5 mg/kg, orally) per day for 1, 3, & 7 days. We also investigated the relationship between lipid peroxidation and ginseng saponins by measuring the levels of superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase (GPx), reduced glutathione(GSH), malondialdehyde (MDA), and hydrogen peroxide(H$_2$O$_2$) in liver tissue. The activities of SOD, CAT, and GPx were generally high in the PPD group; the SOD activity on each day was the highest in the PPD group. The H$_2$O$_2$ content was the lowest in the PPD group. The GSH levels were significantly increased in the PPD. The levels of MDA(the end product of lipid peroxidation) were significantly lower in the red ginseng component groups than in the PQ group; the levels were especially low in the PPD groups. These results led us to conclude that the antioxidant effects of extracts from red ginseng prevent oxidative damage by direct antioxidant effects involving SOD, CAT, & GPx, and increasing the ability of the body to synthesize endogenous antioxidants.

Synthesis of Poly(oxyethylene-co-adipate)-diol from Adipic Acid and Polyethylene Glycols: Effect of Catalyst Concentration (아디프산과 폴리에틸렌글리콜로부터 폴리(옥시에텔렌-아디페이트)-디올 공중합체 합성: 촉매농도의 영향)

  • Jung, Yong-Sung;Lee, Sang-Ho
    • Elastomers and Composites
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    • v.49 no.2
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    • pp.110-116
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    • 2014
  • We investigated the effect of the concentration of stannous 2-ethylhexanoate catalyst on the esterification rate between adipic acid (AA) and each of two PEG oligomers, diethylene glycol (DEG) and polyethylene glycol (PEG600). The concentration of the catalyst was varied from 0.15 to 2.0 wt.%. To attach hydroxy group to each end of the poly(oxyethylene-co-adipate) synthesized from AA and the PEGs, the esterification was performed with excessive PEG oligomers ([PEG]/[AA]=2) at $170^{\circ}C$. The degree of polymerization of the poly(oxyethylene-co-adipate)diol products were three. The apparent rate constant ($k_{app}$) of the esterification between AA and DEG shows the first order dependency on the catalyst concentration ($k_{app}=0.88[C_{cat}]$), whereas the $k_{app}$ of the esterification between AA and PEG600 has a relation of $k_{app}=0.123[C_{cat}]^{0.55}$ with the catalyst concentration. It is expected that the rate of esterification between AA and DEG has a non-linear dependency on the catalyst concentration as the catalyst concentration approaches to 0.22M.

HALO EMISSION OF THE CAT’S EYE NEBULA, NGC 6543: SHOCK EXCITATION BY FAST STELLAR WINDS

  • Hyung, Siek;Lee, Seong-Jae
    • Journal of Astronomy and Space Sciences
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    • v.19 no.3
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    • pp.173-180
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    • 2002
  • Images taken with the Chandra X-ray telescope have for the the first time revealed the central, wind-driven, hot bubble (Chu et al. 2001), while Hubble Space Telescope (HST) WFPC2 images of the Cat's Eye nebula, NGC 6543, show that the temperature of the halo region of angular radius ~ 20", is much higher than that of the inner bright H II region. With the coupling of a photoionization calculation to a hydrodynamic simulation, we predict the observed 〔O III〕 line intensities of the halo region with the same O abundance as in the core H II region: oxygen abundance gradient does not appear to exist in the NGC 6543 inner halo. An interaction between a (leaky) fast stellar wind and halo gas may cause the higher excitation temperatures in the halo region and the inner hot bubble region observed with the Chandra X-ray telescope.