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http://dx.doi.org/10.5142/JGR.2004.28.1.005

Antioxidative Effects of Red Ginseng Saponins on Paraquat-induced Oxidative Stress  

Kim, Dong-Jo (Hannong Chemicals Institute)
Seong, Kum-Soo (Department of Chemistry, Kunsan National University)
Kim, Dong-Won (Department of Chemistry, Kunsan National University)
Kim, Seong-Ruyong (Korea Ginseng Research Institute)
Chang, Che-Chul (Department of Chemistry, Kunsan National University)
Publication Information
Journal of Ginseng Research / v.28, no.1, 2004 , pp. 5-10 More about this Journal
Abstract
This study was carried out to investigate the effect of the active ingredients from ginseng on paraquat(PQ) toxicity. Oxidative stress was induced by intraperitreatneal injection of PQ at a single dose of 25 mg/kg. Saponin treated groups were given protopanaxadiol saponins(PPD) or protopanaxatriol saponins(PPT)(5 mg/kg, orally) per day for 1, 3, & 7 days. We also investigated the relationship between lipid peroxidation and ginseng saponins by measuring the levels of superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase (GPx), reduced glutathione(GSH), malondialdehyde (MDA), and hydrogen peroxide(H$_2$O$_2$) in liver tissue. The activities of SOD, CAT, and GPx were generally high in the PPD group; the SOD activity on each day was the highest in the PPD group. The H$_2$O$_2$ content was the lowest in the PPD group. The GSH levels were significantly increased in the PPD. The levels of MDA(the end product of lipid peroxidation) were significantly lower in the red ginseng component groups than in the PQ group; the levels were especially low in the PPD groups. These results led us to conclude that the antioxidant effects of extracts from red ginseng prevent oxidative damage by direct antioxidant effects involving SOD, CAT, & GPx, and increasing the ability of the body to synthesize endogenous antioxidants.
Keywords
Ginseng; SOD; catalase; GSH; MDA; antioxidant; Protopanaxadiol; Protopanaxatriol;
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