• Animal Bioscience
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• 제35권6호
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• pp.791-803
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• 2022

Genome/gene-editing (GE) techniques, characterized by a low technological barrier, high efficiency, and broad application among organisms, are now being employed not only in medical science but also in agriculture/veterinary science. Different engineered CRISPR/Cas9s have been identified to expand the application of this technology. In pig production, GE is a precise new breeding technology (NBT), and promising outcomes in improving economic traits, such as growth, lean or healthy meat production, animal welfare, and disease resistance, have already been documented and reviewed. These promising achievements in porcine gene editing, including the Myostatin gene knockout (KO) in indigenous breeds to improve lean meat production, the uncoupling protein 1 (UCP1) gene knock-in to enhance piglet thermogenesis and survival under cold stress, the generation of GGTA1 and CMP-N-glycolylneuraminic acid hydroxylase (CMAH) gene double KO (dKO) pigs to produce healthy red meat, and the KO or deletion of exon 7 of the CD163 gene to confer resistance to porcine reproductive and respiratory syndrome virus infection, are described in the present article. Other related approaches for such purposes are also discussed. The current trend of global regulations or legislation for GE organisms is that they are exempted from classification as genetically modified organisms (GMOs) if no exogenes are integrated into the genome, according to product-based and not process-based methods. Moreover, an updated case study in the EU showed that current GMO legislation is not fit for purpose in term of NBTs, which contribute to the objectives of the EU's Green Deal and biodiversity strategies and even meet the United Nations' sustainable development goals for a more resilient and sustainable agri-food system. The GE pigs generated via NBT will be exempted from classification as GMOs, and their global valorization and commercialization can be foreseen.

• 한국시뮬레이션학회논문지
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• 제15권4호
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• pp.11-19
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• 2006

퍼베이시브 컴퓨팅(pervasive computing) 기술이 현실화됨에 따라, 사용자에게 언제 어디서나 맞춤화된 서비스를 제공하기 위한 연구가 확대되고 있다. 기존 연구에서는 주로 단일 서비스가 센서 정보를 처리하는 제한된 방향으로 맞춤화된 서비스를 제공하고 있어, 사용자의 다양한 요구에 적응적인 서비스를 제공하는데 한계가 있다. 본 연구에서는 이와 같은 문제점을 해결하기 위해 시뮬레이션 기반 상황 인식 웹 서비스(CAS: Context-Aware Web services) 개발 방법론을 제안한다. 제안된 방법론은 사용자 상황 정보(예. 위치), 웹서비스 상황 정보(예. 반응시간), 사용 기기의 상태(예. 가용성), 환경 정보(예. 센서 데이터) 등을 시뮬레이션을 통해 종합적으로 고려하여 사용자 환경에 적응적인(adaptable) 서비스를 제공하도록 한다.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2002년도 Proceedings of International Symposium on Remote Sensing
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• pp.721-727
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• 2002

Precipitation evapotranspiration and runoff are three key parameters of regional water balance. Problems exist in the traditional methods for calculating such factors , such as explaining of the geographic rationality of spatial interpolating methods and lacking of enough observation stations in many important area for bad natural conditions. With the development of modern spatial info-techniques, new efficient shifts arose for traditional studies. Guided by theories on energy flow and materials exchange within Soil-Atmosphere-Plant Continuant (SPAC), retrieval models of key hydrological parameters were established in the Yellow River basin using CMS-5 and FengYun-2 meteorological satellite data. Precipitation and evapotranspiration were then estimated: (1) Estimating tile amount of solar energy that is absorbed by the ground with surface reflectivity, which is measured in the visible wavelength band (VIS): (2) Assessing the partitioning of the absorbed energy between sensible and latent heat with the surface temperature, which was measured in the thermal infrared band (TIR), the latent heat representing the evapotranspiration of water; (3) Clouds are identified and cloud top levels are classified using both VIS and TIR data. Hereafter precipitation will be calculated pixel by pixel with retrieval model. Daily results are first obtained, which are then processed to decade, monthly and yearly products. Precipitation model has been has been and tested with ground truth data; meanwhile, the evapotranspiration result has been verified with Large Aperture Scintillometry (LAS) presented by Wageningen University of the Netherlands. Further studies may concentrate on the application of models, i.e., establish a hydrological model of the Yellow river basin to make the accurate estimation of river volume and even monitor the whole hydrological progress.

• 인터넷정보학회논문지
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• 제4권4호
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• pp.53-64
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• 2003

사용자 어플리케이션에서 인증서의 검증 과정을 모두 수행하는 것은 복잡하고 시간 소비적인 작업으로서 사용자 측에 상당한 부담을 줄 수 있다. 특히, 인증서의 서명을 검증하는 작업은 인증 경로상의 모든 인증서들에 대한 암호화 계산을 필요로 하기 때문에 이로 인한 연산적인 부담은 전체 검증 작업 부담의 대부분을 차지하게 된다. 본 논문에서는 인증서 검증 작업 중 서명 검증 작업을 PKI 영역내의 CA들에게 위임함으로서 사용자측의 검증 작업에 대한 부담을 줄일 수 있는 DSVP(Delegated Signature Validation Protocol)를 이용한 새로운 인증서 검증 기법을 제안하였다. 제안한 DSVP는 사용자와 CA들 간의 수행되는 프로토콜로서, 계층적 PKI에서 효율적으로 적용되며 서명 검증 위임 작업이 안전하고 신뢰성 있게 수행되도록 한다. 제안한 기법은 사용자 측의 암호화 계산을 줄이고 상위 CA들을 검증 작업에 가담시킴으로서, 사용자 측의 검증 작업에 대한 부담을 줄임과 동시에 PKI 영역의 정적인 CA들의 활용도를 높이는 효과를 가져올 수 있다.

• 한국수정란이식학회지
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• 제33권4호
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• pp.245-254
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• 2018

Pigs are considered as optimal donor animal for the successful xenotransplantation. To increase the possibility of clinical application, genetic modification to increase compatibility with human is an important and essential process. Genetic modification technique has been developed and improved to produce genetically modified pigs rapidly. CRISPR/Cas9 system is widely used in various fields including the production of transgenic animals and also can be enable multiple gene modifications. In this study, we developed new gene targeting vector and enrichment system for the rapid and efficient selection of genetically modified cells. We conducted co-transfection with two targeting vectors for simultaneous inactivation of two genes and enrichment of the genetically modified cells using MACS. After this efficient enrichment, genotypic analysis of each colony showed that colonies which have genetic modifications on both genes were confirmed with high efficiency. Somatic cell nuclear transfer was conducted with established donor cells and genetically modified pigs were successfully produced. Genotypic and phenotypic analysis of generated pigs showed identical genotypes with donor cells and no surface expression of ${\alpha}$-Gal and HD antigens. Furthermore, functional analysis using pooled human serum revealed dramatically reduction of human natural antibody (IgG and IgM) binding level and natural antibody-mediated cytotoxicity. In conclusion, the constructed vector and enrichment system using MACS used in this study is efficient and useful to generate genetically modified donor cells with multiple genetic alterations and lead to an efficient production of genetically modified pigs.

• 한국전기전자재료학회논문지
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• 제22권5호
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• pp.397-404
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• 2009

Effects of ceramic filler types and dose on the low temperature sintering and dielectric properties of ceramic/$CaO-Al_2O_3-SiO_2$ (CAS) glass composites were investigated. All of the specimens were sintered at $850{\sim}900^{\circ}C$ for 2 h, which conditions are required by the low-temperature co-firing ceramic (LTCC) technology. Ceramic fillers of $CaCO_3$, $Al_2O_3$, $CaCO_3-Al_2O_3$ mixture, and $CaCO_3-Al_2O_3$ compound ($CaAl_2O_4$), respectively, were used. The addition of $Al_2O_3$ yielded the crystalline phase of alumina, which was associated with the inhibition of sintering, while, $CaCO_3$ resulted in no apparent crystalline phase but the swelling was significant. The additions of $CaCO_3-Al_2O_3$ mixture and $CaAl_2O_4$, respectively, yielded the crystalline phases of alumina and anorthite, and the sintering properties of both composites increased with the increase of filler addition and the sintering temperature. In addition, the $CaAl_2O_4$/CAS glass composite, sintered at $900^{\circ}C$, demonstrated good microwave dielectric properties. In overall, all the investigated fillers of 10 wt% addition, except $CaCO_3$, yielded reasonable sintering (relative density, over 93 %) and low dielectric constant (less than 5.5), demonstrating the feasibility of the investigated composites for the application of the LTCC substrate materials.

• 전자공학회논문지
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• 제54권2호
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• pp.3-8
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• 2017

본 논문은 차량 내부에서 무선 에너지 전송 기술을 지원하기 위해 분산 안테나 시스템의 사용을 제안한다. 차량 내 무선 에너지 전송 시스템은 에너지 수신 단말기의 이동이 제한적이고, 채널 환경이 정적이며, 인체가 송신기와 가까운 특징을 갖는다. 이러한 상황에서 송신기의 배치는 인체에서의 수신 에너지와 에너지 수신 단말기에서의 수신 에너지의 양에 큰 영향을 미친다. 이에 본 논문은 기존 무선 통신 시스템에서 사용되는 중앙 집중형 안테나 시스템과 분산 안테나 시스템을 적용하였을 때의 차량 내 무선 에너지 전송 성능을 모의실험을 통해 비교하고, 분산 안테나 시스템이 더 좋은 성능을 가짐을 보인다.

• BMB Reports
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• 제57권1호
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• pp.50-59
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• 2024

The application of gene engineering in livestock is necessary for various reasons, such as increasing productivity and producing disease resistance and biomedicine models. Overall, gene engineering provides benefits to the agricultural and research aspects, and humans. In particular, productivity can be increased by producing livestock with enhanced growth and improved feed conversion efficiency. In addition, the application of the disease resistance models prevents the spread of infectious diseases, which reduces the need for treatment, such as the use of antibiotics; consequently, it promotes the overall health of the herd and reduces unexpected economic losses. The application of biomedicine could be a valuable tool for understanding specific livestock diseases and improving human welfare through the development and testing of new vaccines, research on human physiology, such as human metabolism or immune response, and research and development of xenotransplantation models. Gene engineering technology has been evolving, from random, time-consuming, and laborious methods to specific, time-saving, convenient, and stable methods. This paper reviews the overall trend of genetic engineering technologies development and their application for efficient production of genetically engineered livestock, and provides examples of technologies approved by the United States (US) Food and Drug Administration (FDA) for application in humans.

• 대한원격탐사학회지
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• 제37권5_1호
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• pp.1215-1227
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• 2021

저궤도 위성영상 정보의 절대 대기 보정 처리에 의하여 산출되는 지표 반사도는 정확한 식생 분석에 필요한 기본 자료이다. Committee on Earth Observation Satellites (CEOS)는 사용자가 바로 활용할 수 있도록 하는 지표 반사도의 분석 대기 자료(Analysis Ready Data: ARD) 구축과 관련한 연구와 지침 개발이 수행되고 있다. 그러나 이러한 동향은 중저해상도 위성영상을 대상으로 하고 있어서 KOMPSAT-3A나 CAS-500과 같은 고해상도 분광 영상의 ARD를 다루는 연구는 아직 초기 단계이다. 이 연구는 우선 기존 사례를 바탕으로 하여 ARD 자료의 배포 방식을 정리하였다. 그리고 클라우드 환경에서 운영되는 위성 정보 응용 플랫폼 중의 하나인 오픈 데이터 큐브(Open Data Cube: ODC)와 ARD 자료와의 연계성을 설명하였다. 연구의 결과로 고해상도 위성영상의 실무적인 ARD 구축 단계와 ODC와 클라우드 환경에서 배포되는 고해상도 위성영상의 ARD 구축 모델과 몇 가지 유형의 개념적 수준의 응용 모델을 제시하였다. 한편 제시한 구축과 응용 모델에 대하여 데이터 가격 정책, 정확도 품질 문제, 플랫폼 적용성 문제, 클라우드 환경 문제, 국제 교류 이슈 등을 토의 사항에서 정리하였다. 지구관측 위성과 관계된 주요 국제기구인 Group on Earth Observations (GEO)와 CEOS등에서 ARD와 ODC의 확산을 위한 시스템 기술과 표준 개발을 지속하고 있으며 이러한 성과는 민간부문으로 확대되고 있다. 따라서 우리나라도 이러한 국제 추세에 대한 대응 전략을 마련할 필요가 있다.

• 한국해양바이오학회지
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• 제1권1호
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• pp.40-47
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• 2006

Large scale marine biodiversity studies in China have been carried out for more than half a century since the founding of the Institute of Oceanology, CAS, in 1950. Through a series of coastal and multi-disciplinary oceanographic investigations in the shelf seas and biodiversity studies since the late fifties, a total of 20,278 species of marine biota have been recorded upto 1994. Recent intensive studies have further revealed the richness of marine biota of the China seas,a great number of marine species have been found and many new taxa established. The total species number of main biotic groups increased about 50 % of that estimated in 1994. The results have promoted the fast development of China's marine fisheries, medicine (drug) and biodiversity research, and attracted many scientists, particularly bio-technologists, to join their studies. Environmental deterioration and human activity strongly stressed the sustainable development and conservation of marine bio-diversity, and resulted in the increase of end angered species as record ed in the new published ${\ll}$China Species Red List${\gg}$ with the threatened category of species assessed by adopting the new IUCN criteria. To further reveal the high diversity and their history, present status and future of marine organisms existed in the world ocean, an international Project ${\ll}$Census of Marine Life (CoML)${\gg}$ was established in 2000 in the USA. Scientists predicted that 2 to 3 times of numbers of the known species will possibly be found in various marine habitats, particularly the abyssal ocean. The Research Plan and the Projects were briefly introduced, and the relationship between marine biodiversity and biotechnology was discussed. The Project planned to apply new and high techniques and new equipments on board research vessel and in laboratory. Brief review of recent advances of Chinas' marine biodiversity and biotechnology studies indicated that fascinate results have been achcieved; but further effort should be made to promote the continuous advance of our basic researches and their application in related production and maintain sustainable development.