• Animal Bioscience
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• v.35 no.6
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• pp.791-803
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• 2022

Genome/gene-editing (GE) techniques, characterized by a low technological barrier, high efficiency, and broad application among organisms, are now being employed not only in medical science but also in agriculture/veterinary science. Different engineered CRISPR/Cas9s have been identified to expand the application of this technology. In pig production, GE is a precise new breeding technology (NBT), and promising outcomes in improving economic traits, such as growth, lean or healthy meat production, animal welfare, and disease resistance, have already been documented and reviewed. These promising achievements in porcine gene editing, including the Myostatin gene knockout (KO) in indigenous breeds to improve lean meat production, the uncoupling protein 1 (UCP1) gene knock-in to enhance piglet thermogenesis and survival under cold stress, the generation of GGTA1 and CMP-N-glycolylneuraminic acid hydroxylase (CMAH) gene double KO (dKO) pigs to produce healthy red meat, and the KO or deletion of exon 7 of the CD163 gene to confer resistance to porcine reproductive and respiratory syndrome virus infection, are described in the present article. Other related approaches for such purposes are also discussed. The current trend of global regulations or legislation for GE organisms is that they are exempted from classification as genetically modified organisms (GMOs) if no exogenes are integrated into the genome, according to product-based and not process-based methods. Moreover, an updated case study in the EU showed that current GMO legislation is not fit for purpose in term of NBTs, which contribute to the objectives of the EU's Green Deal and biodiversity strategies and even meet the United Nations' sustainable development goals for a more resilient and sustainable agri-food system. The GE pigs generated via NBT will be exempted from classification as GMOs, and their global valorization and commercialization can be foreseen.

• Journal of the Korea Society for Simulation
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• v.15 no.4
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• pp.11-19
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• 2006

With the emergence of pervasive computing, personalization becomes an important issue to provide with users customized services, anywhere and anytime in their specific environment. Many researches have shown the possibilities of personalization by acquiring and processing sensor information around users. However, personalization remains still at its infancy, since most researches have failed to consider various contexts comprehensively besides sensor data, and just developed tailored services for a specific application domain. In this work, we propose a simulation-based CAS (context Aware Web Services) development methodology. Our methodology considers various contexts on users (eg. current location), web services (eg. response time), devices (eg. availability) and environment (eg. sensor data) all together by simulating them on the fly for personalized and adaptable services.

• Proceedings of the KSRS Conference
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• 2002.10a
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• pp.721-727
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• 2002

Precipitation evapotranspiration and runoff are three key parameters of regional water balance. Problems exist in the traditional methods for calculating such factors , such as explaining of the geographic rationality of spatial interpolating methods and lacking of enough observation stations in many important area for bad natural conditions. With the development of modern spatial info-techniques, new efficient shifts arose for traditional studies. Guided by theories on energy flow and materials exchange within Soil-Atmosphere-Plant Continuant (SPAC), retrieval models of key hydrological parameters were established in the Yellow River basin using CMS-5 and FengYun-2 meteorological satellite data. Precipitation and evapotranspiration were then estimated: (1) Estimating tile amount of solar energy that is absorbed by the ground with surface reflectivity, which is measured in the visible wavelength band (VIS): (2) Assessing the partitioning of the absorbed energy between sensible and latent heat with the surface temperature, which was measured in the thermal infrared band (TIR), the latent heat representing the evapotranspiration of water; (3) Clouds are identified and cloud top levels are classified using both VIS and TIR data. Hereafter precipitation will be calculated pixel by pixel with retrieval model. Daily results are first obtained, which are then processed to decade, monthly and yearly products. Precipitation model has been has been and tested with ground truth data; meanwhile, the evapotranspiration result has been verified with Large Aperture Scintillometry (LAS) presented by Wageningen University of the Netherlands. Further studies may concentrate on the application of models, i.e., establish a hydrological model of the Yellow river basin to make the accurate estimation of river volume and even monitor the whole hydrological progress.

• Journal of Internet Computing and Services
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• v.4 no.4
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• pp.53-64
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• 2003

To perform the certificate validation on the user-side application induces the very considerable overhead on the user-side system because of the complex and time-consuming characteristic of the validation processing. Most of the time spend for performing the validation processing is required for the digital signature verification, since the verification accompanies with the cryptographic calculation over each certificate on the certificate path. In this paper, we propose a new certificate validation scheme using DSVP(Delegated Signature Validation Protocol) which can reduce the overhead for the user-side certificate validation processing. It is achieved by delegating the digital signature verification to CAs of the PKI domain. As the proposed DSVP is the protocol performed between a user and CAs, it is applied to the hierarchical PKI efficiently and used for delegating the digital signature verification reliably and safely, our proposed scheme can not only reduces the overhead for the validation processing by decreasing the cryptographic calculation but also improves the utilization of CAs by employing them to the validation processing.

• Journal of Embryo Transfer
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• v.33 no.4
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• pp.245-254
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• 2018

Pigs are considered as optimal donor animal for the successful xenotransplantation. To increase the possibility of clinical application, genetic modification to increase compatibility with human is an important and essential process. Genetic modification technique has been developed and improved to produce genetically modified pigs rapidly. CRISPR/Cas9 system is widely used in various fields including the production of transgenic animals and also can be enable multiple gene modifications. In this study, we developed new gene targeting vector and enrichment system for the rapid and efficient selection of genetically modified cells. We conducted co-transfection with two targeting vectors for simultaneous inactivation of two genes and enrichment of the genetically modified cells using MACS. After this efficient enrichment, genotypic analysis of each colony showed that colonies which have genetic modifications on both genes were confirmed with high efficiency. Somatic cell nuclear transfer was conducted with established donor cells and genetically modified pigs were successfully produced. Genotypic and phenotypic analysis of generated pigs showed identical genotypes with donor cells and no surface expression of ${\alpha}$-Gal and HD antigens. Furthermore, functional analysis using pooled human serum revealed dramatically reduction of human natural antibody (IgG and IgM) binding level and natural antibody-mediated cytotoxicity. In conclusion, the constructed vector and enrichment system using MACS used in this study is efficient and useful to generate genetically modified donor cells with multiple genetic alterations and lead to an efficient production of genetically modified pigs.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.22 no.5
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• pp.397-404
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• 2009

Effects of ceramic filler types and dose on the low temperature sintering and dielectric properties of ceramic/$CaO-Al_2O_3-SiO_2$ (CAS) glass composites were investigated. All of the specimens were sintered at $850{\sim}900^{\circ}C$ for 2 h, which conditions are required by the low-temperature co-firing ceramic (LTCC) technology. Ceramic fillers of $CaCO_3$, $Al_2O_3$, $CaCO_3-Al_2O_3$ mixture, and $CaCO_3-Al_2O_3$ compound ($CaAl_2O_4$), respectively, were used. The addition of $Al_2O_3$ yielded the crystalline phase of alumina, which was associated with the inhibition of sintering, while, $CaCO_3$ resulted in no apparent crystalline phase but the swelling was significant. The additions of $CaCO_3-Al_2O_3$ mixture and $CaAl_2O_4$, respectively, yielded the crystalline phases of alumina and anorthite, and the sintering properties of both composites increased with the increase of filler addition and the sintering temperature. In addition, the $CaAl_2O_4$/CAS glass composite, sintered at $900^{\circ}C$, demonstrated good microwave dielectric properties. In overall, all the investigated fillers of 10 wt% addition, except $CaCO_3$, yielded reasonable sintering (relative density, over 93 %) and low dielectric constant (less than 5.5), demonstrating the feasibility of the investigated composites for the application of the LTCC substrate materials.

• Journal of the Institute of Electronics and Information Engineers
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• v.54 no.2
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• pp.3-8
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• 2017

This paper considers an application of distributed antenna system (DAS) to a system of intra-vehicle wireless energy transfer (WET). The intra-vehicle WET system has features such as limited mobility of energy receiver, static channel environment and short distance between transmitter and human body. Under these conditions, location of transmitter highly affects the amount of energy received by human body and the energy received by energy receiving devices. We compare centralized antenna system (CAS) and the DAS in intra-vehicle WET system by simulation. The results show the DAS has superior performance to the CAS.

• BMB Reports
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• v.57 no.1
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• pp.50-59
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• 2024

The application of gene engineering in livestock is necessary for various reasons, such as increasing productivity and producing disease resistance and biomedicine models. Overall, gene engineering provides benefits to the agricultural and research aspects, and humans. In particular, productivity can be increased by producing livestock with enhanced growth and improved feed conversion efficiency. In addition, the application of the disease resistance models prevents the spread of infectious diseases, which reduces the need for treatment, such as the use of antibiotics; consequently, it promotes the overall health of the herd and reduces unexpected economic losses. The application of biomedicine could be a valuable tool for understanding specific livestock diseases and improving human welfare through the development and testing of new vaccines, research on human physiology, such as human metabolism or immune response, and research and development of xenotransplantation models. Gene engineering technology has been evolving, from random, time-consuming, and laborious methods to specific, time-saving, convenient, and stable methods. This paper reviews the overall trend of genetic engineering technologies development and their application for efficient production of genetically engineered livestock, and provides examples of technologies approved by the United States (US) Food and Drug Administration (FDA) for application in humans.

• Korean Journal of Remote Sensing
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• v.37 no.5_1
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• pp.1215-1227
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• 2021

Surface reflectance, as a product of the absolute atmospheric correction process of low-orbit satellite imagery, is the basic data required for accurate vegetation analysis. The Commission on Earth Observation Satellite (CEOS) has conducted research and guidance to produce analysis-ready data (ARD) on surface reflectance products for immediate use by users. However, this trend is still in the early stages of research dealing with ARD for high-resolution multispectral images such as KOMPSAT-3A and CAS-500, as it targets medium- to low-resolution satellite images. This study first summarizes the types of distribution of ARD data according to existing cases. The link between Open Data Cube (ODC), the cloud-based satellite image application platforms, and ARD data was also explained. As a result, we present practical ARD deployment steps for high-resolution satellite images and several types of application models in the conceptual level for high-resolution satellite images deployed in ODC and cloud environments. In addition, data pricing policies, accuracy quality issue, platform applicability, cloud environment issues, and international cooperation regarding the proposed implementation and application model were discussed. International organizations related to Earth observation satellites, such as Group on Earth Observations (GEO) and Committee on Earth Observation Satellites (CEOS), are continuing to develop system technologies and standards for the spread of ARD and ODC, and these achievements are expanding to the private sector. Therefore, a satellite-holder country looking for worldwide markets for satellite images must develop a strategy to respond to this international trend.

• Journal of Marine Bioscience and Biotechnology
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• v.1 no.1
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• pp.40-47
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• 2006

Large scale marine biodiversity studies in China have been carried out for more than half a century since the founding of the Institute of Oceanology, CAS, in 1950. Through a series of coastal and multi-disciplinary oceanographic investigations in the shelf seas and biodiversity studies since the late fifties, a total of 20,278 species of marine biota have been recorded upto 1994. Recent intensive studies have further revealed the richness of marine biota of the China seas,a great number of marine species have been found and many new taxa established. The total species number of main biotic groups increased about 50 % of that estimated in 1994. The results have promoted the fast development of China's marine fisheries, medicine (drug) and biodiversity research, and attracted many scientists, particularly bio-technologists, to join their studies. Environmental deterioration and human activity strongly stressed the sustainable development and conservation of marine bio-diversity, and resulted in the increase of end angered species as record ed in the new published ${\ll}$China Species Red List${\gg}$ with the threatened category of species assessed by adopting the new IUCN criteria. To further reveal the high diversity and their history, present status and future of marine organisms existed in the world ocean, an international Project ${\ll}$Census of Marine Life (CoML)${\gg}$ was established in 2000 in the USA. Scientists predicted that 2 to 3 times of numbers of the known species will possibly be found in various marine habitats, particularly the abyssal ocean. The Research Plan and the Projects were briefly introduced, and the relationship between marine biodiversity and biotechnology was discussed. The Project planned to apply new and high techniques and new equipments on board research vessel and in laboratory. Brief review of recent advances of Chinas' marine biodiversity and biotechnology studies indicated that fascinate results have been achcieved; but further effort should be made to promote the continuous advance of our basic researches and their application in related production and maintain sustainable development.