• Title/Summary/Keyword: CAPE

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Caffeic acid phenethyl ester protects against photothrombotic cortical ischemic injury in mice

  • Hwang, Sun Ae;Kim, Chi Dae;Lee, Won Suk
    • The Korean Journal of Physiology and Pharmacology
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    • v.22 no.1
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    • pp.101-110
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    • 2018
  • In this study, we aimed to investigate the neuroprotective effects of caffeic acid phenethyl ester (CAPE), an active component of propolis purified from honeybee hives, on photothrombotic cortical ischemic injury in mice. Permanent focal ischemia was achieved in the medial frontal and somatosensory cortices of anesthetized male C57BL/6 mice by irradiation of the skull with cold light laser in combination with systemic administration of rose bengal. The animals were treated with CAPE (0.5-5 mg/kg, i.p.) twice 1 and 6 h after ischemic insult. CAPE significantly reduced the infarct size as well as the expression of tumor necrosis $factor-{\alpha}$, hypoxiainducible $factor-1{\alpha}$ monocyte chemoattractant protein-1, $interleukin-1{\alpha}$, and indoleamine 2,3-dioxygenase in the cerebral cortex ipsilateral to the photothrombosis. Moreover, it induced an increase in heme oxygenase-1 immunoreactivity and interleukin-10 expression. These results suggest that CAPE exerts a remarkable neuroprotective effect on ischemic brain injury via its anti-inflammatory properties, thereby providing a benefit to the therapy of cerebral infarction.

Multiple Ovulations and In vitro Fertilization in the Domestic Fowl (Gallus domesticus)

  • Han, Haitang;Zhao, Chen;Li, Zandong
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.12
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    • pp.1652-1656
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    • 2004
  • The aim of this study was to obtain mature ova or embryos at a single cell stage, which can be used in avian transgenesis and nuclear transfer through multiple ovulations, in vitro fertilization and culture. Chicken anterior pituitary extract (CAPE) or acetone-dried chicken anterior pituitary extract (ACAPE) was used to induce multiple ovulations in hens pretreated with pregnant mare' serum gonadotrophin (PMSG). In vitro fertilization of the multiple ovulated ova was performed by inseminating sperm onto the germinal disks in m-Ringer' solution and incubating the ova at 41$^{\circ}C$, 5% $CO_2$ for 10 h in DME-F12 medium containing 20% liquid albumen. The in vitro fertilization process was observed using an environmental scanning electron microscope. When normal laying hens (white Leghorn) were administered daily with PMSG (100 IU), egg laying ceased in most hens within 3 to 8 days. Ovulation began to occur about 7.5 h after injection of CAPE and ACAPE. The number of ovulated ova was 1.00${\pm}$0.00, 2.33${\pm}$0.52 and 2.20${\pm}$0.45, respectively, after receiving 100, 200 and 300 mg CAPE. The number of ovulated ova was 2.00${\pm}$0.00, 2.86${\pm}$0.69 and 3.00${\pm}$1.22, respectively, after receiving 10, 15 and 20 mg ACAPE. The fertilized and cultured ova were able to develop into embryos up to the 32 cell stage. The present experiments demonstrated that multiple ovulations can be induced by CAPE and ACAPE successfully, and the ova resulted from the treatment retained the capability for further fertilization and embryonic development. These data provide new information to support the establishment of an in vitro culture system for future avian transgenesis studies.

A Study of Correlation Between Acoustic and Perceptual Parameters in the Patients with Vocal Polyp (성대용종 음성에 대한 음향지표와 청지각지표의 상관관계 연구)

  • Lee, Hyun Doo;Jeon, Yi Seul;Hong, Ki Hwan
    • Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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    • v.26 no.1
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    • pp.40-45
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    • 2015
  • Objectives:This study aims to investigate the correlation between the measurements of Praat as an acoustic evaluation and those of GRBAS and CAPE-V as perceptual rating tool respectively. Through this, it also tries to find out parameters to which attention should be paid when an evaluator, who is untrained in auditory-perceptual voice evaluation, conducts voice evaluation with objective tool. Materials and Methods:Voice samples of this study were 33 vocal polyp patients(23 males and 10 females) who visited our Department of Otorhinolaryngology. They sustained vowel voices of 'e' were recorded and acoustically analyzed. Results:As the results of correlation analysis between GRBAS and Praat measurements, G scale and R scale showed statistically significant correlation with Jitt, Shim and NHR. And it is found that B scale represented significant correlation with Jitt, S scale with Shim. As the results of analysis on correlation with CAPE-V and Praat measurements, OS scale and R scale showed statistically significant correlation with Jitt, Shim and NHR. B scale represented significant correlation with Jitt, S scale with Shim. Conclusion:Although, both GRBAS and CAPE-V were highly reliable, in comparison between CAPE-V and Pratt, more parameters that showed statistically significant correlation are observed, which implies that VAS has more potential to make detailed evaluation than ORD.

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Computational predictions of improved of wall mechanics and function of the infarcted left ventricle at early and late remodelling stages: comparison of layered and bulk hydrogel injectates

  • Kortsmit, Jeroen;Davies, Neil H.;Miller, Renee;Zilla, Peter;Franz, Thomas
    • Advances in biomechanics and applications
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    • v.1 no.1
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    • pp.41-55
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    • 2014
  • Acellular intra-myocardial biomaterial injections have been shown to be therapeutically beneficial in inhibiting ventricular remodelling of myocardial infarction (MI). Based on a biventricular canine cardiac geometry, various finite element models were developed that comprised an ischemic (II) or scarred infarct (SDI) in left ventricular (LV) antero-apical region, without and with intra-myocardial biomaterial injectate in layered (L) and bulk (B) distribution. Changes in myocardial properties and LV geometry were implemented corresponding to infarct stage (tissue softening vs. stiffening, infarct thinning, and cavity dilation) and injectate (infarct thickening). The layered and bulk injectate increased ejection fraction of the infarcted LV by 77% (II+L) and 25% (II+B) at the ischemic stage and by 61% (SDI+L) and 63% (SDI+B) at the remodelling stage. The injectates decreased the mean end-systolic myofibre stress in the infarct by 99% (II+L), 97% (II+B), 70% (SDI+L) and 36% (SDI+B). The bulk injectate was slightly more effective in improving LV function at the remodelling stage whereas the layered injectate was superior in functional improvement at ischemic stage and in reduction of wall stress at ischemic and remodelling stage. These findings may stimulate and guide further research towards tailoring acellular biomaterial injectate therapies for MI.

Analysis of Wind Environments at Homi-Cape, Pohang (포항 호미곶의 풍환경 분석)

  • Kim Hyun-Goo;Choi Jae-Ou;Jung Woo-Sik
    • Proceedings of the KSME Conference
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    • 2002.08a
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    • pp.451-454
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    • 2002
  • In the present paper, the practicability of Commentray on Wind Loads of Korean Standard Design Loads fur Buildings, which has been enacted in Minstry of Construction and Transportation in June 2000, is validated by using the meteorological data measured at Homi-Cape, Pohang. Assuming that the vertical wind profile follows the power-law in a quasi-steady state, wind profile exponents are calculated by seasons by using wind data as collected through four monitoring towers. According to the Commentray on Wind Loads, Pohang is classified with its exposure category being B and the wind profile exponent being 0.22, while it is identified that the average wind profile exponent as calculated in this study is 0.26. Also, in this paper, a cross-correlation method is suggested in order to identify any meteorological correlation between measurement sites quantitatively.

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Modelling and integrity assessment of shear connectors in precast cast-in-situ concrete bridges

  • Moyo, Pilate;Sibanda, Bongani;Beushausen, Hans
    • Structural Engineering and Mechanics
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    • v.42 no.1
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    • pp.55-72
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    • 2012
  • Precast-cast insitu concrete bridge construction is widely practiced for small to medium span structures. These bridges consist of precast pre-stressed concrete beams of various cross-sections with a cast in-situ reinforced concrete slab. The connection between the beams and the slab is via shear links often included during the manufacturing process of the beams. This form of construction is attractive as it provides for standardisation, reduced formwork and construction time. The assessment of the integrity of shear connectors in existing bridges is a major challenge. A procedure for assessment of shear connectors based on vibration testing and finite element model updating is proposed. The technique is applied successfully to a scaled model bridge model and an existing bridge structure.

Inhibition of Angiogenesis by Propolis

  • Song, Yun-Seon;Park, Eun-Hee;Jung, Kyung-Ja;Jin, Changbae
    • Archives of Pharmacal Research
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    • v.25 no.4
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    • pp.500-504
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    • 2002
  • Propolis, obtained from honeybee hives, has been used in Oriental folk medicine as an anti-inflammatory, anti-carcinogenic, and immunomodulatory agent. There is considerable evidence suggesting that angiogenesis and chronic inflammation are codependent. Blockage of angiogenesis results in an anti-inflammatory effect. Ethanol (EEP) and ether extracts of propolis (REP), and caffeic acid phenethyl ester (CAPE), an active component of propolis, were examined for their anti-angiogenic activities using the chick embryo chorioallantoic membrane (CAM), and the calf pulmonary arterial endothelial (CPAE) cell proliferation, assays. The presence of EEP, REP and CAPE inhibited angiogenesis in the CAM assay and the proliferation of CPAE cells. The results suggest that anti-angiogenic activities of EEP, REP and CAPE are also responsible for their anti-inflammatory effect.

Anticancer Effects of Thymoquinone, Caffeic Acid Phenethyl Ester and Resveratrol on A549 Non-small Cell Lung Cancer Cells Exposed to Benzo(a)pyrene

  • Ulasli, Sevinc Sarinc;Celik, Sefa;Gunay, Ersin;Ozdemir, Mehmet;Hazman, Omer;Ozyurek, Arzu;Koyuncu, Tulay;Unlu, Mehmet
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.10
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    • pp.6159-6164
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    • 2013
  • Background: Phytochemical compounds are emerging as a new generation of anticancer agents with limited toxicity in cancer patients. The purpose of this study was to investigate the potential effcts of thymoquinone, caffeic acid phenylester (CAPE) and resveratrol on inflammatory markers, oxidative stress parameters, mRNA expression levels of proteins and survival of lung cancer cells in Vitro. Materials and Methods: The A549 cell line was treated with benzo(a)pyrene, benzo(a)pyrene plus caffeic acid phenylester (CAPE), benzo(a)pyrene plus resveratrol (RES), and benzo(a)pyrene plus thymoquinone (TQ). Inflammatory markers, oxidative stress parameters, mRNA expression levels of apoptotic and anti-apoptotic proteins and cell viability were assessed and results were compared among study groups. Results: TQ treatment up-regulated Bax and down-regulated Bcl2 proteins and increased the Bax/Bcl2 ratio. CAPE and TQ also up-regulated Bax expression. RES and TQ down-regulated the expression of Bcl-2. All three agents decreased the expression of cyclin D and increased the expression of p21. However, the most significant up-regulation of p21 expression was observed in TQ treated cells. CAPE, RES and TQ up-regulated TRAIL receptor 1 and 2 expression. RES and TQ down-regulated the expression of NF-kappa B and IKK1. Viability of CAPE, RES and TQ treated cells was found to be significantly decreased when compared with the control group (p=0.004). Conclusions: Our results revealed up-regulation of the key upstream signaling factors, which ultimately cause increase in their regulatory p53 levels affecting the induction of G2/M cell cycle arrest and apoptosis. Overall these results provide mechanistic insights for understanding the molecular basis and utility of the anti-tumor activity of TQ, RES and CAPE.

The Validity of Activity Participation Assessment for School-Age Children (학령기 아동을 위한 활동 참여 평가도구(Activity Participation Assessment)의 타당도 연구)

  • Kim, Se-Yun
    • The Journal of Korean Academy of Sensory Integration
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    • v.17 no.1
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    • pp.19-29
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    • 2019
  • Objective : The aim of this study was to verify validity of the Activity Participation Assessment for school-age children. Methods : A questionnaire consisting of 30 items from the APA, 75 items from the PACS, and 55 items from the CAPE was administered to elementary school students. A total of 207 questionnaires were analyzed. Confirmatory factor analysis was performed to confirm the construct validity of the APA. Convergence validity and discriminant validity were verified with the average variance extracted (AVE) and the square of the correlation coefficient. The discriminant validity was the Pearson correlation coefficient of the APA, PACS, and CAPE. Results : The results of the analysis were as follows: 1) For construct validity, the goodness of fit of the modified hierarchical second-order factor model was found to be appropriate (p < .001), 2) For convergent validity, the AVE was higher than .50 for all latent variables, 3) For discriminant validity, the AVE of the latent variable was greater than the square of the correlation coefficient (0.239), 4) For concurrent validity, the correlation between the total sum of the APA and PACS scores showed a positive correlation in all domains, and the correlation coefficient ranged from .303 to .647 at a statistically significant level (p < .01), 5) The correlation coefficient between the total sum of the APA and CAPE scores was .490 for recreational activities, .329 for physical activities, .571 for social activities, .401 for skill-based activities, and .390 for self-improvement activities. All domains showed a positive correlation, and were statistically significant (p < .01). Conclusion : APA can be used as a valid assessment tool to measure the participation of school aged children.

Analysis of Wind Environments for Siting a Wind Farm (풍력발전 단지조성을 위한 바람환경 분석)

  • 김현구;최재우;손정봉;정우식;이화운
    • Journal of Korean Society for Atmospheric Environment
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    • v.19 no.6
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    • pp.745-756
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    • 2003
  • An analysis of wind environments using computational fluid dynamics and an evaluation of wind resources using measurement data obtained from meteorological observation sites at Homi-Cape, Pohang have been carrid out for siting a wind farm. It was shown that a numerical simulation using computational fluid dynamics would provide reliable wind resource map in complex terrain with land-sea breeze condition. As a result of this investigation, Homi-Cape wind farm with 11.25 ㎿ capacity has been designed for maximum power generation and 25.7 GWh electricity production is predicted.