• Journal of Microbiology and Biotechnology
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• v.26 no.9
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• pp.1586-1592
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• 2016

Klebsiella pneumoniae is a gram-negative, non-motile, rod-shaped, and encapsulated bacterium in the normal flora of the intestines, mouth, skin, and food, and has decarboxylation activity, which results in generation of diamines (cadaverine, agmatine, and putrescine). However, there is no specific information on the exact mechanism of decarboxylation in K. pnuemoniae. Specifically lysine decarboxylases that generate cadaverine with a wide range of applications has not been shown. Therefore, we performed a functional study of lysine decarboxylases. Enzymatic characteristics such as optimal pH, temperature, and substrates were examined by overexpressing and purifying CadA and LdcC. CadA and LdcC from K. pneumoniae had a preference for L-lysine, and an optimal reaction temperature of 37℃ and an optimal pH of 7. Although the activity of purified CadA from K. pneumoniae was lower than that of CadA from E. coli, the activity of K. pneumoniae CadA in whole cell bioconversion was comparable to that of E. coli CadA, resulting in 90% lysine conversion to cadaverine with pyridoxal 5'-phosphate L-lysine.

• Korean Journal of Computational Design and Engineering
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• v.1 no.2
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• pp.133-149
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• 1996

In order to reduce the trial-and-errors in design and production of injection molded plastic parts, there has been much research effort not only on CAE systems which simulate the injection molding process, but also on CAD systems which support initial design and re-design of plastic parts and their molds. The CAD systems and CAE systems have been developed independently with being built on different basis. That is, CAD systems manipulate the part shapes and the design features in a complete solid model, while CAE systems work on shell meshes generated on the abstract sheet model or medial surface of the part. Therefore, it is required to support the two types of geometric models and feature information in one environment to integrate CAD and CAE systems for accelerating the design speed. A feature-based non-manifold geometric modeling system has been developed to provide an integrated environment for design and analysis of injection molding products. In this system, the geometric models for CAD and CAE systems are represented by a non-manifold boundary representation and they are merged into a single geometric model. The suitable form of geometric model for any application can be extracted from this model. In addition, the feature deletion and interaction problem of the feature-based design system has been solved clearly by introducing the non-manifold Boolean operation based on 'merge and selection' algorithm. The sheet modeling capabilities were also developed for easy modeling of thin plastic parts.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.15 no.2
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• pp.141-148
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• 1997

Continuous development of Personal Computer(PC) combined with the decrease of price and the expansion of operating system in PC like Windows 95, and Windows N/T makes it possible for the user to consider PC-bas-ed Automated Happing/Facilities Management (AM/FM) system. Therefore, the purpose of this study is to establish a PC-based data automation system of pipe infrastructures-water, sewer, and drainage-and road in Korea Land Corporation. In order to achieve this goal, relevant literature survey was done first. Secondly, the study site was determined by discussion with Korea Land Institute. Thirdly, PC-based CAD software for AM/FM in the world market were surveyed, and AutoCAD Map was selected because KLC had been using hundreds of AutoCAD copies already. After that user needs assessment was done to visit on-site office, local branch office, and the corresponding city office for the system design and database design. After the graphic data were digitized and the attribute data were entered into the batabase, the data automation system was established, and tested for application. Finally, the guidelines and problems for PC-based data automation in AM/FM was discussed.

• Proceedings of the Korean Institute Of Construction Engineering and Management
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• autumn
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• pp.187-192
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• 2001

High-Rise Building Construction Project which has high uncertainty and complexity, causes lots of problems under construction. In particular, the integration between design phase and construction phase highly affects a construction project in terms of cost and time. To possibly make the flexible response and preliminary verification against the construction planning problems in high rise building project, this paper proposes the Engineering-Construction Management System based on 4D CAD that put the time factor together with 3D CAD. The usefulness of this system has been verified through the actual project. It could helps the reducing trials and errors during construction phase by catching the factors on problems of project. Ultimately it will allow users to save project cost and duration by field application.

• Journal of the Korean Academy of Esthetic Dentistry
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• v.22 no.1
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• pp.22-29
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• 2013

In general, to conduct aesthetic restorations, various analyses are carried out using a camera from the initial examination with the final prosthesis in consideration. With the reality that many dentists are using digital cameras, it can be considered that the time of digital impression has already started. Just as the recent general trend is that more and more internationally renowned photographers are switching their film cameras to digital counterparts, it is likely that CAD/CAM will show another direction in the area of the aesthetic dentistry that we may want to pursue. With the word 'digital', the convenience and economics often come to mind in the first place. However, from the dental clinical viewpoint, it is important to improve and develop the CAD/CAM system based on understanding its specialty and superiority while respecting the conventional analog techniques. However, a regretful aspect is that it is often difficult to catch up with the latest advancements for proper referencing and follow-up of digital technologies since the CAD/CAM device and material are developing very rapidly. Accordingly, although it is ideal to have hands-on experiences in various digital material and devices, and adapt to their fast changes, it must be stressed that the clinical application is to be implemented on the basis of the proven traditional way of dental clinics in order to obtain better outcomes. This presentation will explore types of approaches that can be made by combining the traditional techniques and the CAD/CAM from the aesthetic viewpoint. In addition, it is hoped and eagerly awaited that the CAD/CAM restoration may play a significant role in the field of the 'digital art'.

• The Journal of Advanced Prosthodontics
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• v.12 no.1
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• pp.22-32
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• 2020

PURPOSE. The aim of this study was to evaluate the microshear bond strength (µSBS) of four computer-aided design/computer-aided manufacturing (CAD/CAM) blocks repaired with composite resin using three different surface treatment protocols. MATERIALS AND METHODS. Four different CAD/CAM blocks were used in this study: (1) flexible hybrid ceramic (FHC), (2) resin nanoceramic (RNC), (c) polymer infiltrated ceramic network (PICN) and (4) feldspar ceramic (FC). All groups were further divided into four subgroups according to surface treatment: control, hydrofluoric acid etching (HF), air-borne particle abrasion with aluminum oxide (AlO), and tribochemical silica coating (TSC). After surface treatments, silane was applied to half of the specimens. Then, a silane-containing universal adhesive was applied, and specimens were repaired with a composite, Next, µSBS test was performed. Additional specimens were examined with a contact profilometer and scanning electron microscopy. The data were analyzed with ANOVA and Tukey tests. RESULTS. The findings revealed that silane application yielded higher µSBS values (P<.05). All surface treatments were showed a significant increase in µSBS values compared to the control (P<.05). For FHC and RNC, the most influential treatments were AlO and TSC (P<.05). CONCLUSION. Surface treatment is mandatory when the silane is not preferred, but the best bond strength values were obtained with the combination of surface treatment and silane application. HF provides improved bond strength when the ceramic content of material increases, whereas AlO and TSC gives improved bond strength when the composite content of material increases.

• The Transactions of the Korean Institute of Power Electronics
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• v.8 no.3
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• pp.239-246
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• 2003

Brushless D.C. Motor is widely used for industrial application because of high efficiency and high power density. Especially, in home appliance, BLDCM is very useful due to high control performance and low acoustic noise. In this paper, BLDCM and its controller are designed and developed for tread mill application. With the restricted stator structure, permanent magnet rotor is designed for manufacturing and cost effectiveness using CAD and FEM analysis. A ferrite magnetic material is used as a rotor magnet for the cost and temperature advantages. For a stable operation of tread mill, over current and temperature can be detected and protected. The designed BLDCM and its controller was verified by the experimental results.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 2006.04a
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• pp.208-215
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• 2006

This study provides a new operation strategy which can guarantee the data consistency of engineering information among the various intelligent information systems. We present the strategies for the operation of bridges engineering information and the construction methodology of integrated database. The two core standard techniques are adopted to construct the integrated database. One of these standards is the Standard for the Exchange of Product Model Data (STEP) for CAD/CAE information and the other is the Extensible Markup Language (XML) for engineering document information. This study can transform a document me into a data type for web-based application modules which assist end-users in searching and retrieval of engineering document data. In addition, relaying algorithm is developed to integrate the two different information, e.g. CAD/CAE information and engineering document information. The pilot application modules for management and maintenance of existing bridge are also developed to show application of the strategy.

• Journal of The Korean Digital Architecture Interior Association
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• v.2 no.1
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• pp.9-15
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• 2002

As architectural forms tend to be large-scaled, high-storied and complicated, use of computer for processing design information has been generalized. However, CAD use in the process of developing architectural design has been neglected greatly in the educational field of architectural field due to the surging of recognition that it may limit the essential property of architectural design. It may be resulted from the limitation of CAD system, that is, it is because of tardy speed of application development for the related areas with the lack of simplicity and clarity of flexibility to be secured on the drawing and user interface. Recent CAD systems, however, overcome such a limitation, convert into object-based design from entity-based drawing, drafting and modeling for implementing design concept of architects and it connects with internet linked to superspeed information communication network and changes process and stream of architectural design. Therefore, this study deals with utility of object based CAD system with products of Autodesk Co. and consequently obtain the following conclusions. First, it expands architect's design areas by supporting cooperative design system based on model-based architectural design and internet. Second, it reduces consumption of personal and material resources and time in the process of drawing production for improving architectural design works. Third, it can reduce the frequent design changes by improving understanding of architectural space with visualization of immediate 3D information, escaping from traditional methods to deliver design information to building owner through 2D drawing or model and perspective drawing requiring much cost and time. Fourth, it keeps exactness without omission or duplication of design information and generate and renew information of all related drawings on a drawing. Fifth, it is possible to change difficult and boring architectural design work into a pleasure owing to immediate modeling and drawing of design idea. In addition, digital drawing generated by using object-based CAD system can playa role of establishing Urban Information System to be used for protecting from building in disaster and urban disasters in connection with GIS numerical map and be used for reference of all kinds of information required fro post-management of buildings. However, what is more important is that surplus time from introduction of object-based CAD system should be used for consideration to be recognized as a good space and building built as a product of this project for comfort to urban people.

• Journal of Energy Engineering
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• v.25 no.1
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• pp.108-112
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• 2016

This study is established the data-centric design concept, which is the latest design technique, by analyzing existing study literature for its application on the nuclear power plant industry in Korea. This study investigated the data-centric design cases in the advanced companies and suggests a data-centric design integrated system framework by analyzing the major functions of the commercial 3D CAD system, which is globally used in the plant architect engineering. In order to apply the data-centric design integrated system framework to the nuclear power plant industry in Korea, the main functions of a nuclear power plant design information integrated system framework, which can manage the design products of each EPC step and the related information in integrated way, is suggested by analyzing the supplier design, field design process and field design drawings, which have close relation with the plant Architect Engineering (A/E). It is expected that the result of this study would contribute in the dramatic enhancement in the job efficiency of nuclear power plant design process in Korea.