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A Study on the Prediction of the Permanent Wilting Point in Woody Plant by Cambial Electrical Resistance (목본식물의 형성층 전기저항에 의한 영구위조점 예측에 관한 연구)

  • 김민수
    • Journal of the Korean Institute of Landscape Architecture
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    • v.22 no.4
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    • pp.75-80
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    • 1995
  • It is important to estimate the possibility of recovery in physiologically damaged woody plant. It is suggested that C.E.R(cambial electrical resistance) might be a useful method to predict the permanent wilting point. D/A and A/D converter can be used to measure the C.E.R and it took only 10-20 msec for a measurement and the values were stable during this study. A computer could be used for the continual measurement of C.E.R. There were very big daily changes of C.E.R. was changed according to the changes of indoor temperature, but the phase was slightly different. It is reasoned that daily changes in C.E.R. is induced by the changes of water potential and cambial thickness. It was difficult to detect the changes of C.E.R. caused by changes in soil moisture under high soil water potential. Under low soil water potential, the changes in soil moisture under high soil water potential. Under low soil water potential, the changes of C.E.R. can be detected. After wilting, C.E.R. is increased very rapidly. When C.E.R. is not decreased by watering, it will be permanent wilting point. But it takes several days to confirm the permanent wilting point. To predict the possibility of recovery from wilting, the values of C.E.R. have no meaning. But the changes of C.E.R. are significant. Therefore we can predict the permant wilting point in woody plant by monitoring the change of C.E.R. by the computer.

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Molecular Cloning and Characterization of ${\gamma}-tocopherol$ Methyltransferase cDNA from Perilla frutescens (들깨 ${\gamma}-tocopherol$ methyltransferase cDNA 유전자의 분리 및 특성)

  • Hwang, Seon-Kap;Kim, Dong-Hern;Lee, Jai-Youl;Kim, Young-Hwan;Hwang, Young-Soo;Kim, Kyung-Hwan
    • Applied Biological Chemistry
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    • v.45 no.4
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    • pp.203-206
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    • 2002
  • ${\gamma}-Tocopherol$ methyltransferase (TMT) is an enzyme catalyzing ${\gamma}-tocopherol$ into ${\alpha}-tocopherol$ at the final step of ${\alpha}-tocopherol$ synthesis pathway. Putative TMT cDNA clone specific to Perilla frutescens immature seeds was isolated from cDNA library. The cDNA clone consisted of 1369 bp open reading frame encoding 369 amino acids with a relative Mw of 42 kDa. Results revealed the CDNA has 60% homology to Arabidopsis thaliana TMT, and possesses methyltransferase and S-adenosyl methionine-binding domains, suggesting that cDNA encodes a ${\gamma}-tocopherol$ methyltransferase To characterize the properties of the TMT gene, the cDNA sequences coding for mature TMT were expressed in E. coli and assayed to determine the enzyme activity in vitro.

Effects of Seed Storage Methods and GA3 Application on Seed Germination and Seedling Growth of Solanum lyratum Thunb. (종자저장방법 및 GA3처리가 배풍등 종자 발아와 유묘 생육에 미치는 영향)

  • Lee, Su Gwang;Kim, Hyo Yun;Ku, Ja Jung
    • Korean Journal of Plant Resources
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    • v.27 no.4
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    • pp.365-370
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    • 2014
  • This study was conducted to determine the effects of seed storage methods ($-20^{\circ}C$, stratification, $2^{\circ}C$ dry, $2^{\circ}C$ wet and room temperature) and $GA_3$ application (control, $dH_2O$, 10, 100, 1000 ppm) on seed germination and seedling growth of S. lyratum. As a result seed germination rate of S. lyratum was the highest at 91% when seeds were stored at room temperature and then soaked for 24 hours in $GA_3$ 10 ppm. And seedlings of S. lyratum showed the best quality when seed were stored at $2^{\circ}C$ dry and then soaked for 24 hours in $GA_3$ 1000 ppm, with the growth characteristics of plant height (47 mm), number of leaves (8), leaf width (12 mm), leaf length (19 mm), fresh weight (aerial/root part; 471/476 mg), dry weight (aerial/root; 106/41 mg) and seedling quality indices (106). Therefore, S. lyratum seed were stored at $2^{\circ}C$ dry, and then soaked for 24 hours in $GA_3$ 1000 ppm, seed germination rate was more than 80% and production of superior quality container seedlings.

Transposable Elements and Genome Size Variations in Plants

  • Lee, Sung-Il;Kim, Nam-Soo
    • Genomics & Informatics
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    • v.12 no.3
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    • pp.87-97
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    • 2014
  • Although the number of protein-coding genes is not highly variable between plant taxa, the DNA content in their genomes is highly variable, by as much as 2,056-fold from a 1C amount of 0.0648 pg to 132.5 pg. The mean 1C-value in plants is 2.4 pg, and genome size expansion/contraction is lineage-specific in plant taxonomy. Transposable element fractions in plant genomes are also variable, as low as ~3% in small genomes and as high as ~85% in large genomes, indicating that genome size is a linear function of transposable element content. Of the 2 classes of transposable elements, the dynamics of class 1 long terminal repeat (LTR) retrotransposons is a major contributor to the 1C value differences among plants. The activity of LTR retrotransposons is under the control of epigenetic suppressing mechanisms. Also, genome-purging mechanisms have been adopted to counter-balance the genome size amplification. With a wealth of information on whole-genome sequences in plant genomes, it was revealed that several genome-purging mechanisms have been employed, depending on plant taxa. Two genera, Lilium and Fritillaria, are known to have large genomes in angiosperms. There were twice times of concerted genome size evolutions in the family Liliaceae during the divergence of the current genera in Liliaceae. In addition to the LTR retrotransposons, non-LTR retrotransposons and satellite DNAs contributed to the huge genomes in the two genera by possible failure of genome counter-balancing mechanisms.

Growth Characters and Vegetation Survey in Natural Habitat of Dicentra spectabilis (L.) Lem (금낭화의 생육특성 및 자생지 식생조사)

  • Heo, Kwon;Lee, Chae-Gon;Jang, Min-Young;Cho, Dong-Ha;Yoo, Chang-Yeon
    • Korean Journal of Plant Resources
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    • v.13 no.3
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    • pp.188-194
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    • 2000
  • This study was carried out to obtain the optimum cultivation conditions of wild ornamental plant resources, Dicentra spectabilis L. The altitude of the habitat was ranged from 630m to 690m in Mt. Gujeol. Most of the habitat faced toward the northeast aspect and the gradient was ranged from 25 to 33 degrees. The light intensity of Dicentra community was 14,000lux on the average, and it was tendency to decrease by foliation of other woody plants. The appearance number of Dicentra per quadrat was 30 individuals on the average, and total appearance species in all quadrat surveyed was 52 taxa. Among them, Quercus mongolica, Lindera obtusiloba, Corylus heterophylla, Cornus controversa, woody plants, Corydalis speciosa, Pseudostellaria heterophylla, Artemisia keiskeana, Dryopteris crassirhizoma, Arisaema amurense var. serratum, Carex siroumensis, and Chloranthus japonicus, herbaceous plants, were showed as dominant species. Soil acidity in the habitat was 5.15 to 5.96, and average content of soil moisture was 32.6%. The contents of average organic matter, $P_2O_5$, K, Ca, and Mg were 1.99%, 14mg/kg, 0.55me/100g, 15.2me/100g, and 3.3me/100g, respectively. Electron conductivity was ranged from 0.50 to 0.76dS/m. Also, the average air temperature of May and June was 14.2$^{\circ}C$ and 19.4$^{\circ}C$, respectively.

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Study on the Response of Korean Ginseng (Panax ginseng C.A. Meyer) to the Herbicide 2,4-D Application (고려인삼에 대한 제초제 2, 4-D의 반응연구)

  • Jo, Jae-Seong
    • Journal of Ginseng Research
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    • v.14 no.2
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    • pp.291-296
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    • 1990
  • Various rates of 2,4-D were sprayed on 2 and 3 year old ginseng plants as foliar spray to define the critical concentration. No apparent plant injury was noticable for those ginseng plants when application concentration of 2,4-D doubled the recommended dosage (70 ml/10a). Neither abnormal foliar change occurred nor any inhibition in leaf and stem growth was resulted for the plants treated with 2,4-D concentrated two times of the recommended dosage. When the rates of 2,4-D application were increased greater than this level, injury ratings increased linearly with the rates of 2,4-D application and plant you was inhibited. Ethylene gas was not produced from the ginseng plant treated with 2 times concentrated 2,4-D, however the ginseng plants produced 0.03 to 0.09 ppm ethylene gas when the rate of application were increased 3 and 4 times, respectively. On the other hand the soybean treated with the recommended amount of 21-D produced ethylene gas of 10-20 times higher compared with ginseng plants and died. Photosynthesis ability of the ginseng leaf was significantly decreased by 2,4-D foliar application but it was recovered 4 weeks after 2,4-D foliar treatment. The herbicide 2,4-D was applicated to 2,3 and 4 years old ginseng plants as foliar spray with the rates of 0.5, 1.0, 1.5 and 2.0 times of the recommended dosage to define the effects of 2,4-D on the plant growth and root yield of the ginseng. There were no significant differences in the leaf and stem growth between untreated and 2,4-D treated plant. Berry maturing of 3 and 4 year old ginseng was not influenced by 2,4-D. The root weight of 4 years old ginseng plant was not reduced by application of 2,4-D concenrated 2 times of the recommended dosage, Application time of the herbicide 2,4-D had no effects on the leaf or stem growth of 2,3 and 4 year old year old ginseng plants. When the ginseng seedling was treated with 2,4-D, detrimental phenomena as stem bending and docoration of seedling leaf margin occurred, but stem bending was recovered in a few day s. Keywords Panax ginseng C.A. Meyer, 2,4-D , herbicide.

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DNA Methylase and Chloroplast DNA Methylation in Chlamydomonas (Chlamydomonas에서 분리한 DNA Methylase와 엽록체 DNA Methylation)

  • 김남곤
    • Journal of Plant Biology
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    • v.35 no.4
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    • pp.415-423
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    • 1992
  • Chlamydomonas reinhardtii 21 gr(mt+) strain의 배우체로부터 두 종류의 DNA methylase를 부분 분리하여 몇가지 기질 DNA에 대한 효소 활성을 측정하였다. DNA methylase I과 II는 동일한 pH와 ionic strength에서 서로 상이한 물리적인 성질과 서로 다른 분자량을 가지며 DNA methylase I과 II는 모두가 DNA 염기 중 adenine보다는 cytosine에 methylation을 수행하는 것으로 생각된다. 합성 DNA를 사용한 실험에서 DNA methylase I과는 달리 DNA methylase II는 poly(dA-dC)·poly(dG-dT)에서 보다 poly(dG-dC)·poly(dG-dC)의 oligonucleotide에서 더 높은 효소활성을 나타내었다. Chlamydomonas reinhardtii에서 추출한 엽록체 DNA를 기질로 사용하였을 때 DNA methylase I과 II 모두가 배우체기 보다는 영양생장기의 엽록체 DNA에 더 높은 활성을 나타내었다.

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Change of Constituent Components in Selected Korean Chestnut (Castanea crenata S. et Z.) Cultivars by Different Storage Conditions (국내산 밤 일부 품종의 다른 저장 조건들에 의한 성분변화)

  • Kim, Dae-Jung;Chung, Mi-Ja;Seo, Dong-Joo;You, Jin-Kyoun;Shim, Tae-Heum;Choe, Myeon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.2
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    • pp.225-234
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    • 2009
  • The aim of this study was to analyze moisture, crude protein, crude lipid, vitamin C and sugar changes in selected Korean chestnut cultivars such as Danteack, Deabo, Seokchu, Okkwang and Byunggo during storage at $4^{\circ}C$ and $-10^{\circ}C$ for 10 months. The moisture contents of selected Korean chestnut cultivars ranged from 49.9 to 57.4%. The moisture content of Seokchu was the highest. The contents of moisture in white kernel were higher than that in yellow kernel. The content of moisture showed decreasing tendency after 10 months of storage. The crude protein and crude lipid contents in whole kernel of selected Korean chestnut cultivars were $3.3{\sim}4.2%$ and $0.3{\sim}1.6%$, respectively. The crude protein content of Deabo was the highest. The crude protein in Danteack, Seokchu, Okkwang and Byunggo was increased during storage at $4^{\circ}C$ for 10 months, while that in whole kernel of Deabo was decreased and no changes in crude protein in yellow kernels of Deabo were observed. The crude protein in Okkwang was increased during storage at $-10^{\circ}C$ for 10 months. The cold storage was found to have higher composition change of crude protein than the freezing storage. The content of crude lipid in Daebo and Byunggo was decreased during storage at $4^{\circ}C$ and $-10^{\circ}C$. Yellow kernels of Deabo, Okkwang and Byunggo were found to have higher crude lipid content than white kernels. The vitamin C content also decreased during storage at $4^{\circ}C$ and $-10^{\circ}C$ and the decrease in vitamin C content was higher at $4^{\circ}C$ than $-10^{\circ}C$. Vitamin C was not detected after 3 months storage at $-10^{\circ}C$. The sugar content increased at the latter period storage at $4^{\circ}C$ and $-10^{\circ}C$. The sugar content of selected Korean chestnut cultivars ranged from $36.2{\sim}44.3%$ and Dantaek had the highest sugar content.

A Characteristic Analysis of Physical, Chemical and Electrical Property for Bunker C Fly Ash (Bunkder C유 회분의 물리적, 화학적, 전기적 특성분석)

  • 이재근;이정언;안영철
    • Proceedings of the Korea Society for Energy Engineering kosee Conference
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    • 1996.04a
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    • pp.88-96
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    • 1996
  • The characteristic analysis of fly ash generated from a fired power plant using bunker-C oil has been investigated. Ash size distribution by an optical microscopy with image processing technique, morphological shape by a scanning electron microscope(SEM) and microscope, chemical composition by the inductively coupled plasma emission spectrometry(ICP), and resistivity measurement as a function of temperature and moisture content by the resistivity meter are performed. A study of physical, chemical and electrical characteristics of bunker-C fly ash plays an important role of improving the performance of an electrostatic precipitator and protecting air pollution. The samples of bunker-C fly ash for analysis were collected from the electrostatic precipitator hopper of Ulsan Power Plant Unit 1 and Pusan Power Plant Unit 1. Mass median diameter(MMD) of bunker-C fly ash was measured 12.7${\mu}{\textrm}{m}$, while MMD of fly ash generated from the mixture of bunker-C oil(40%) and domestic anthracitic coal(60%) was 25.7${\mu}{\textrm}{m}$. The morphological structure of bunker-C fly ash consisted of fine particles of non-spherical shape. The primary chemical components of bunker-C fly ash were composed of SiO2(2.36%), Al2O3(4.91%), Fe2O3(14.33%) and C(11.84%). Resistivity of bunker-C fly ash was found to be increased with increasing temperature at the range of 100~15$0^{\circ}C$ and was measured 103~104 ohm-cm.

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A study on the application of the extreme value distribution model for analysis of probability of exceeding the facility capacity (시설용량을 초과하는 폐수량의 유입확률 분석을 위한 극치분포모델의 적용에 관한 연구)

  • Choi, Sunghyun;Yoo, Soonyoo;Park, Taeuk;Park, Kyoohong
    • Journal of Korean Society of Water and Wastewater
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    • v.30 no.4
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    • pp.369-379
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    • 2016
  • It was confirmed that the extreme value distribution model applies to probability of exceeding more than once a day monthly the facility capacities using data of daily maximum inflow rate for 7 wastewater treatment plant. The result of applying the extreme value model, A, D, E wastewater treatment plant has a problem compared to B, C, F, G wastewater treatment plant. but all the wastewater treatment plant has a problem except C, F wastewater treatment plant based 80% of facility capacity. In conclusion, if you make a standard in statistical aspects probability exceeding more than once a day monthly can be 'exceed day is less than a few times annually' or 'probability of exceeding more than once a day monthly is less than what percent'.