• The Korean Journal of Physiology and Pharmacology
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• v.22 no.6
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• pp.689-696
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• 2018

Increasing evidence implicates changes in $[Ca^{2+}]_i$ and oxidative stress as causative factors in amyloid beta ($A{\beta}$)-induced neuronal cell death. Cyanidin-3-glucoside (C3G), a component of anthocyanin, has been reported to protect against glutamate-induced neuronal cell death by inhibiting $Ca^{2+}$ and $Zn^{2+}$ signaling. The present study aimed to determine whether C3G exerts a protective effect against $A{\beta}_{25-35}$-induced neuronal cell death in cultured rat hippocampal neurons from embryonic day 17 fetal Sprague-Dawley rats using MTT assay for cell survival, and caspase-3 assay and digital imaging methods for $Ca^{2+}$, $Zn^{2+}$, MMP and ROS. Treatment with $A{\beta}_{25-35}$ ($20{\mu}M$) for 48 h induced neuronal cell death in cultured rat pure hippocampal neurons. Treatment with C3G for 48 h significantly increased cell survival. Pretreatment with C3G for 30 min significantly inhibited $A{\beta}_{25-35}$-induced $[Zn^{2+}]_i$ increases as well as $[Ca^{2+}]_i$ increases in the cultured rat hippocampal neurons. C3G also significantly inhibited $A{\beta}_{25-35}$-induced mitochondrial depolarization. C3G also blocked the $A{\beta}_{25-35}$-induced formation of ROS. In addition, C3G significantly inhibited the $A{\beta}_{25-35}$-induced activation of caspase-3. These results suggest that cyanidin-3-glucoside protects against amyloid ${\beta}$-induced neuronal cell death by reducing multiple apoptotic signals.

• The Korean Journal of Malacology
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• v.24 no.3
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• pp.167-173
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• 2008

The survival and growth of Glossaulax didyma didyma ($R{\ddot{o}}ding$) were measured in the laboratory under controlled conditions, such as water temperature, salinity and diet. The effect of temperature on G. didyma didyma was examined at four different levels (15, 20, 25 and $30^{\circ}C$) with 30 psu for 30 days. The results show that the growth and survival rates of G. didyma didyma were significantly higher at $15^{\circ}C$ and $20^{\circ}C$ than at other temperatures. The effect of salinity on G. didyma didyma was also examined at six different levels (10, 15, 20, 25, 30 and 35 psu) in $20^{\circ}C$ for 40 days. The results showed that the growth and survival rates of G. didyma didyma were significantly higher at 30 psu and 35 psu than at other salinities. Finally, the effect of diets on G. didyma didyma was examined with three single-component diets (prawn, shellfish and fish) at $20^{\circ}C$ and 30 psu for 60 days. The results showed that the growth and survival rates of G. didyma didyma fed on shellfish were significantly higher than those fed on prawn and fish.

• Proceedings of the Acoustical Society of Korea Conference
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• 1998.06c
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• pp.151-154
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• 1998

이중 전송율(5.3 과 6.3kbit/s)을 제공하는 G.723.1 음성 코더는 공중망을 통한 H.324 POTS 영상 회의 규격의 음성 코더로 채택된 것으로, MPMLQ, ACELP 알고리즘에 근거한다. 본 논문에서는 Annex A를 포함한 G.723.1 음성 코더 알고리즘을 C&S Technology에서 개발한 음성 신호 처리를 위한 범용 DSP인 CSD17C00 칩을 이용하여 실시간 응용이 가능하도록 구현하였다. G.723.1 에 대한 양방향 평가가 Codec loopback을 통해 수행되었으며, ITU에서 제공한 테스트 절차에 따라 평가되었다. 또한, 본 논문에서 구현된 G.723.1 음성 코더는 27MIPS의 계산 속도를 갖으며, 프로그램 ROM의 크기는 8.85K Words이고, 10K 데이터 ROM과 4K 데이터 RAM을 필요로 하고 있다. 경쟁 제품과의 MOS 측정 음질 평가를 실시한 결과, CSD17C00에서의 음질 성능이 더 우수함을 입증 함으로써, 본 논문에서 보여준 CSD17C00을 기반으로 구현된 G.723.1 알고리즘의 실시간 구현기술의 타당성을 검증하게 되었다.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.8 no.2
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• pp.78-93
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• 2003

To investigate seasonal variation and structure of the microbial community in Kyeonggi Bay, abundance and carbon biomass of nano-and micrzooplankton were evaluated in relation to size fractionated chlorophyll-a concentration, through the monthly interval sampling from December 1997 to November 1998. Communities of nano-and microzooplankton were classified into 4 groups such as heterotrophic nanoflagellate(HNF), ciliates, heterotrophic dinoflagellates(HDF) and zooplankton nauplii. Abundance and carbon biomass of HNF ranged from 380 to 4,370 cells ml-1(average 1,340$\pm$130 cells ml-1) and from 0.63 to 12.4 $\mu\textrm{g}$C 1-1(average 4.35$\pm$0.58 $\mu\textrm{g}$C 1-1), respectively. Abundance and carbon biomass of ciliates ranged from 331 to 44,571 cells ml-1(average 3,526$\pm$544 cells ml-1) and from 1.3 to 119.7 $\mu\textrm{g}$C 1-1(average 13.7$\pm$3.0 $\mu\textrm{g}$C 1-1), respectively. Abundance and carbon biomass of HDF ranged from 88 to 48,461 cells 1-1(average 9,034$\pm$2,347 cells 1-1) and from 0.05 to 54.05 $\mu\textrm{g}$C 1-1(average 6.9$\pm$2.4 $\mu\textrm{g}$C 1-1), respectively. Abundance and carbon biomass of zooplankton nauplii ranged from 5 to 546 indiv. 1-1(average 83$\pm$15 indiv. 1-1) and from 0.17 to 43.2 $\mu\textrm{g}$C 1-1(average 6.3$\pm$1.2 $\mu\textrm{g}$C 1-1), respectively. Eash component of microbial biomass was not different from tidal cycle except tintinnids group. Depth integrated nano-and microzooplankton biomass ranged from 124 to 1,635 mgC m-2(average 585$\pm$110 mgC m-2) and was highest in March and May. The relative contribution of each component to the nano-and microzooplankton showed difference according to seasons. Community structure of nano-and microzooplankton was dominated by planktonic ciliate group. During the study period, carbon biomass of nano-and microzooplankton was strongly positively correlated with size fractionated chlorophylla-a. It implied that prey-predator relationship between microzooplankton and phytoplankton was important in the pelagic ecosystem of Kyeonggi Bay.

• Journal of Sericultural and Entomological Science
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• v.50 no.2
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• pp.82-86
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• 2012

We have developed processing methods improved cyanidin-3-glucoside(C3G) stability in mulberry fruit's jam. In the processing of mulberry jam, 3 minutes blanching using microwave inhibited the C3G destruction by maintaining the antioxidant capacity and inactivation of enzymes related to pigment's stability. Also citric acid increased C3G stability in the mulberry fruit's jam. As the adding sugar, sucrose was considered the more desirable sugar than oligosaccharide or xylitol. It showed high C3G content and antioxidant capacity compared to the two treatment groups. In conclusion, when processing mulberry fruit's jam, the addition of citric acid and the enzyme's inactivation treatment were considered effective treatment for the C3G stability and antioxidant ability.

• Journal of applied mathematics & informatics
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• v.42 no.3
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• pp.511-520
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• 2024

In this paper, we introduce the concept of split and non-split tree (D, C)- set of a connected graph G and its associated color variable, namely split tree (D, C) number and non-split tree (D, C) number of G. A subset S ⊆ V of vertices in G is said to be a split tree (D, C) set of G if S is a tree (D, C) set and ⟨V - S⟩ is disconnected. The minimum size of the split tree (D, C) set of G is the split tree (D, C) number of G, γ_χST (G) = min{|S| : S is a split tree (D, C) set}. A subset S ⊆ V of vertices of G is said to be a non-split tree (D, C) set of G if S is a tree (D, C) set and ⟨V - S⟩ is connected and non-split tree (D, C) number of G is γ_χST (G) = min{|S| : S is a non-split tree (D, C) set of G}. The split and non-split tree (D, C) number of some standard graphs and its compliments are identified.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.2
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• pp.247-254
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• 2005

The objective of this study is to compare the chemical properties of red ginseng, white ginseng, and extruded ginseng. Six kinds of samples were prepared and examined their chemical components. The comparison among crude ash, crude lipid, and total sugar resulted insignificant difference. White ginseng had lower content of reducing sugar than those of extruded ginseng and red ginseng. Total amino acid was found relatively low in treatment A (sliced whole root and dried at 7$0^{\circ}C$). Total amino acid of treatment C (extruded dry whole root ginseng slices, moisture content 30%, barrel temperature 11$0^{\circ}C$, and screw speed 200 rpm) was higher than that of treatment B (extruded dry whole root slices, moisture content 25%, barrel temperature 11$0^{\circ}C$, and screw speed 200 rpm). Crude saponin of treatments A, B, C, D (white ginseng with skin), E (skinless white ginseng), and F (red ginseng) were 4.02, 4.77, 4.12, 3.56, 3.25, and 4.02%, respectively. Ginsenoside was contained similarly as crude saponin. The amount of ginsenoside in the treatment of A, B, C, D, E, and F was recorded respectively at 6.031, 8.108, 6.876, 7.978, 5.591, and 9.834 mg/g. A specific component in red ginseng, $R_{g3}$ was detected in treatment F. Maltol was detected in treatment Band F. Acidic polysaccharide was increased 2∼3% by extrusion process. In conclusion, extruded ginseng had similar components to those of red ginseng.

• Bulletin of the Korean Mathematical Society
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• v.52 no.3
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• pp.977-986
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• 2015

Let G be a graph on the vertex set $V(G)=\{x_1,{\cdots},x_n\}$ with the edge set E(G), and let $R=K[x_1,{\cdots},x_n]$ be the polynomial ring over a field K. Two monomial ideals are associated to G, the edge ideal I(G) generated by all monomials $x_i,x_j$ with $\{x_i,x_j\}{\in}E(G)$, and the vertex cover ideal $I_G$ generated by monomials ${\prod}_{x_i{\in}C}{^{x_i}}$ for all minimal vertex covers C of G. A minimal vertex cover of G is a subset $C{\subset}V(G)$ such that each edge has at least one vertex in C and no proper subset of C has the same property. Indeed, the vertex cover ideal of G is the Alexander dual of the edge ideal of G. In this paper, for an unmixed bipartite graph G we consider the lattice of vertex covers $L_G$ and we explicitly describe the minimal free resolution of the ideal associated to $L_G$ which is exactly the vertex cover ideal of G. Then we compute depth, projective dimension, regularity and extremal Betti numbers of R/I(G) in terms of the associated lattice.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.1
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• pp.3-7
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• 2005

The insulin-like growth factors (IGFs), their receptors, and their binding proteins play key roles in regulating cell proliferation and apoptosis. Insulin-like growth factor binding protein-3 (IGFBP3, OMIM #146732) is one of the proteins that bind to the IGFs. IGFBP3 is a modulator of IGF bioactivity, and direct growth inhibitor in the extravascular tissue compartment. We identified twenty-two novel single nucleotide polymorphisms (SNPs) in IGFBP3 gene in Korean cattle (Hanwoo, Bos taurus coreanae) by direct sequencing of full gene including -1,500 bp promoter region. Among the identified SNPs, five common SNPs were screened in 650 Korean cattle; one SNP in promoter (IGFBP3 G-854C), one in 5'UTR region (IGFBP3 G-100A), two in intron 1 (IGFBP3 G+421T, IGFBP3 T+1636A), and one in intron 2 (IGFBP3 C+3863A). The frequencies of each SNP were 0.357 (IGFBP3 G-854C), 0.472 (IGFBP3 G-100A), 0.418 (IGFBP3 G+421T), 0.363 (IGFBP3 T+1636A) and 0.226 (IGFBP3 C+3863A), respectively. Haplotypes and their frequencies were estimated by EM algorithm. Six haplotypes were constructed with five SNPs and linkage disequilibrium coefficients (|D'|) between SNP pairs were also calculated. The information on SNPs and haplotypes in IGFBP3 gene could be useful for genetic studies of this gene.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.3
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• pp.338-342
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• 2015

There has been known C3G (Cyanidin-3-Glucoside), D3G (Delphinidin-3-Glucoside), and Pt3G (Petunidin-3-Glucoside) were main anthocyanin pigments in black-seeded soybean. Anthocyanin contents of total 1,032 black-seeded soybean germplasms were analyzed by HPLC. Average of total anthocyanin content was 11.67 mg/g on the all materials ranged from 0.54 to 23.45 mg/g. Mean value of C3G, D3G, and Pt3G contents in all black-seeded soybeans were 8.81, 1.78 and 0.79 mg/g, respectively. Environmental conditions influenced anthocyanin contents during seed development. Delayed flowering, especially of later maturing germplasms, has been showed to result in increased anthocyanin content. So, prolonged maturation period germplasm is generally higher than that of shorten genotypes. It may be concluded that the higher levels of anthocyanin content was associated with the late dates of harvest maturity. Also larger seeds showed high anthocyanin contents than smaller. That inclination is similar in C3G's occasion because C3G content contribute highly to total anthocyanin content than other pigments.