KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Distribution of Anthocyanin Contents According to Growth Stages in Black-seeded Soybean Germplasms

검정콩 유전자원의 생육기별 안토시아닌 함량 분포

  • Lee, Joo-Young (Dep. of Medical Biotechnology, Soonchunhyang Univ.) ;
  • Hwang, In-Taek (Dep. of Medical Biotechnology, Soonchunhyang Univ.) ;
  • Choi, Byung-Ryul (Gyeonggi-do Agricultural Research and Extension Services) ;
  • Yi, Eun-Seob (Gyeonggi-do Agricultural Research and Extension Services) ;
  • Kim, Yong-Ho (Dep. of Medical Biotechnology, Soonchunhyang Univ.)
  • 이주영 (순천향대학교 의료생명공학과) ;
  • 황인택 (순천향대학교 의료생명공학과) ;
  • 최병렬 (경기도농업기술원) ;
  • 이은섭 (경기도농업기술원) ;
  • 김용호 (순천향대학교 의료생명공학과)
  • Received : 2015.07.01
  • Accepted : 2015.09.01
  • Published : 2015.09.30
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Abstract

There has been known C3G (Cyanidin-3-Glucoside), D3G (Delphinidin-3-Glucoside), and Pt3G (Petunidin-3-Glucoside) were main anthocyanin pigments in black-seeded soybean. Anthocyanin contents of total 1,032 black-seeded soybean germplasms were analyzed by HPLC. Average of total anthocyanin content was 11.67 mg/g on the all materials ranged from 0.54 to 23.45 mg/g. Mean value of C3G, D3G, and Pt3G contents in all black-seeded soybeans were 8.81, 1.78 and 0.79 mg/g, respectively. Environmental conditions influenced anthocyanin contents during seed development. Delayed flowering, especially of later maturing germplasms, has been showed to result in increased anthocyanin content. So, prolonged maturation period germplasm is generally higher than that of shorten genotypes. It may be concluded that the higher levels of anthocyanin content was associated with the late dates of harvest maturity. Also larger seeds showed high anthocyanin contents than smaller. That inclination is similar in C3G's occasion because C3G content contribute highly to total anthocyanin content than other pigments.

검정콩 안토시아닌 고함유 유전자원 선발을 위해 총 1,032점의 유전자원을 개화기, 수확기, 등숙일수에 따른 안토시아닌 함량을 분석하였다. 수집된 유전자원의 총 안토시아닌 함량은 평균 11.67 mg/g이었고 범위는 0.54~23.45 mg/g으로 나타났다. 종류별로는 C3G가 평균 8.81 mg/g으로 비중이 가장 컸으며 그 다음이 D3G가 평균 1.78 mg/g이었고 Pt3G가 평균 0.79 mg/g으로 가장 적었다. 총 안토시아닌 함량별 검정콩 유전자원 분포는 10~15 mg/g 범위가 371점으로 가장 많았고 20 mg/g이상인 유전자원은 30점이었다. 생식생장기 동안 생육시기에 따른 안토시아닌 함량은 개화기와 수확기가 늦어질수록 높아지는 경향이었다. 따라서 등숙일수가 길수록 검정콩의 안토시아닌 함량은 높아진다는 것을 알 수 있었다. 이밖에도 종실중이 큰 대립종이 소립종에 비해 안토시아닌 함량이 높은 경향이었다.

Keywords

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