• Journal of Food Hygiene and Safety
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• v.14 no.4
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• pp.327-332
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• 1999

The prevalence and serotype of food-borne pathogens was investigated from 888 samples of chilled meat, 222 samples of packed frozen meat and 117 samples of imported frozen meat during the period from March 1996 to October 1998. Isolation rates of pathogens associated with food poisoning were revealed in order of Staphyloccus aureus, Campylobacter jejuni /coli, Listeria monocytogenes and Salmonella spp, but Escherichia coli O157:H7 was not isolated in all of the meat samples. Amusingly, Campylobacter jejuni /coli were isolated highly in refrigerated meat, but was not isolated in packed frozen meat. L. monocytogenes was encounted higher isolation frequency in packed frozen chicken meat than in refrigerated chicken meat. In the distribution of serotypes of isolates, most isolates of Sta. aureus classified as enterotoxin type C and D. All of the Salmonella spp. isolated from pork were diagnosed group A and most of isolates from chicken meat were grouped B and D. Most of L. monocytogenes isolated from chicken meat were grouped type 1 and a few number of isolates classified as type 4.

• Journal of Microbiology and Biotechnology
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• v.22 no.11
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• pp.1467-1470
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• 2012

We compared rapid fingerprinting using repetitive sequencebased PCR (rep-PCR) for subtyping Campylobacter jejuni isolates to the widely used multilocus sequence typing (MLST). Representative C. jejuni isolates (n = 16) from broilers were analyzed using MLST and rep-PCR. Both techniques demonstrated an equal discriminatory power of 0.8917, and 9 subgroups were identified. Clonal identification of all 16 isolates was identical for both techniques. The rep-PCR as described in this study may be used as a rapid and cost-effective alternative for subtyping of C. jejuni isolates, or as an effective screening tool in large epidemiological studies.

• Korean Journal of Veterinary Research
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• v.27 no.2
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• pp.245-251
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• 1987

A total of 145 strains of thermophilic Campylobacter spp. isolated from the fecal specimens of 108 cattle, 120 pigs and 104 chickens. The isolation rates of Campylobacter jejuni from cattle, pigs and chickens were 36.1%, 38.3% and 28.8%, respectively. In the biotyping of 115 strains of C. jejuni, 49.6% were belonged to biotype I, 33.9% biotype II, 10.4% biotype IV and 6.1 % biotype III. Twenty-eight strains of C. coli were 78.6% of biotype I, 21.4% biotype II. Two strains of C. laridis belonged to biotype I and II. One hundred of 105 C. jejuni cultures were typable serologically and represented 13 serogroups Serotype 4, 5, 26, 27 and 36 were encountered most frequently. Eighteen of 23 C. coli cultures were typable serologically and represented 6 serogroups. Serotype 8, 20, 21 and 31 were encountered most frequently. In the comparison of frequency of serotype between animal species, serotypes 4, 30, 5, 26 and 27 were encountered relatively common in the cattle source isolates, serotypes 26 and 36 in the pigs, and 36 and 17 in the chickens. The serotypes of C. coli encountered most frequently were serotype 8 and 31.

• Preventive Nutrition and Food Science
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• v.12 no.4
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• pp.279-283
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• 2007

Aqueous chlorine dioxide $(ClO_2)$ treatment was used for the inactivation of Listeria monocytogenes and Campylobacter jejuni in chicken. Chicken breasts and legs were inoculated with $8{\sim}9log\;CFU/g$ of Listeria monocytogenes and Campylobacter jejuni, respectively, and then treated with 0, 50, and 100 ppm of $ClO_2$ solution. Aqueous $ClO_2$ treatment decreased the populations of the pathogenic bacteria on the chicken samples. One hundred ppm $ClO_2$ treatment on the chicken breast and leg reduced the populations of Listeria monocytogenes and Campylobacter jejuni by $0.61{\sim}1.93\;and\;0.99{\sim}1.21log\;CFU/g$, respectively. Aqueous $ClO_2$ treatment affected the microbial growth during storage at $4^{\circ}C$ by decreasing the initial microbial populations. These results clearly suggest that aqueous $ClO_2$ treatment should be useful in improving the microbial safety of chicken during storage and extending the shelf life.

• Korean Journal of Veterinary Research
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• v.29 no.3
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• pp.303-313
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• 1989

To investigate the epidemiological trait of intestinal diseases of animals caused by thermophilic Campyllobacter spp., isolation of etiological agent was carried out and the profiles of plasmids and the transfer of resistance plasmid in the isolated Campylobacter spp. were examined. The results were as follows. 1. A total of 110 isolates of C jejuni and C coli were subjected to the test for the presence of plasmid DNA. Of the isolates examined, 60% of the isolates were noted to harbor plasmid DNA. Plasmid occurrencer ate from pigs, chickens and cattle were 76.2%, 61.7% and 37.7%, respectively. The plasmids of a large molecular weight, ranging from 36 Md to 86Md, were identified with the strains of tetracycline resistant. 2. Transfer frequency of tetracycline resistant plasmids was higher in the case of the filter mating method than in the broth mating method by the factor of 10~1,000. 3. Tetracycline resistant plasmids of C jejuni were transferrable to C jejuni and C coli by conjugation. In a low frequency, the transfer of tetracycline plasmid was also possible to Vibrio parahemolyticus. However, it was impossible to transfer to Streptococcus fecalis, E coli and Vibrio cholerae. 4. Tetracycline resistant plasmids of C jejuni were impossible to transfer to Campylobacter spp. and related bacteria by transformation.

• Bulletin of the Korean Chemical Society
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• v.25 no.2
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• pp.289-292
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• 2004

A trisaccharide, GlcNAcp- ${\beta}-(1{\to}3)-Galp-{\alpha}-(1{\to}2)$-6-deoxy-altroHepp- ${\alpha}-(1{\to}O$-propyl, as an O-antigenic repeating unit of C. jejuni serotype O:23 and O:36 was synthesized. Coupling of the GlcNPhth-(1${\to}$3)-Gal disaccharide donor with allyl 6-deoxy-altroHep acceptor in the presence of iodonium dicollidine perchlorate (IDCP) promoter afforded the ${\alpha}$-galactosidic trisaccharide with high stereoselectivities. Subsequent deacetalation, dephthaloylation, N-acetylation, and hydrogenolytic debenzylation furnished the title compound.

• Archives of Pharmacal Research
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• v.27 no.2
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• pp.143-150
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• 2004

A trisaccharide, the O-antigenic repeating unit of C. jejuni serotype O:23 and O:36, was synthesized as a 2 -azidoethyl glycoside by block addition of perbenzylated thiogalactoside donors to $\alpha$-altroHepp-(1$\rightarrow$3)-GlcNPhth disaccharide acceptor in presence of IDCP promoter. The $\alpha$-linked altroheptopyranoside moiety in the glycosyl acceptor was effectively prepared by Swern oxidation of $\alpha$-mannohepp-(1$\rightarrow$3)-GlcNPhth disaccharide followed by mild reduction with1 $NaCNBH_3$.

• Korean Journal of Microbiology
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• v.54 no.1
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• pp.31-37
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• 2018

Campylobacter jejuni is an important food-borne pathogen causing gastroenteritis in human. We isolated 208 strains of Campylobacter jejuni from 430 diarrhea patients and food employees with 17 food-poisoning outbreaks between 2014 and 2016 in Gyeonggi area. The strains were tested for genetic relationship and the genotype distribution using PFGE and multiplex-PCR typing. Among the 47 Penner-serotypes known for C. jejuni, it was identified as a genotype consisting of 35 genotypes by multiplex-PCR typing and represented 7 genotypes (HS2, HS4A, HS8, HS15, HS29, HS41, and HS53) in the selected strain. From the PFGE analysis of 11 food-poisoning outbreaks, 5 group of PFGE profile were obtained, and genetic similarity in these clusters ranged from 61.8 to 66.6%. This study examines the genetic diversity of C. jejuni that have been separated in the Gyeonggi area through various genetic analysis methods and identifies the correlation between strains in patients who have been infected with the disease in the future.

• Korean Journal of Veterinary Service
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• v.38 no.3
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• pp.167-171
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• 2015

Pathogenic microorganisms were monitored on the chicken carcasses in slaughterhouse of Jeonbuk area. It was conducted to evaluate the microbiological quality on 204 chicken carcasses. Staphylococcus (S.) aureus was isolated in largest number and its ration was 41.2%, Salmonella spp. 6.4%, Campylobacter (C.)jejuni 7.4%, C. coli 7.4%. Serotype of Salmonella (S.) spp. was identified as S. Infantis 46.1%, S. Enteritidis 23.1%, S. Typhimurium 7.7%, S. Montevideo 7.7%. In breed chickens, Salmonella spp. was detected broiler 4.1%, white semi-broiler 8.0% Korean native chicken 12.0%. C. jejuni was isolated broiler 7.4%, white semi-broiler 12.0%, Korean native chicken 0%, C. coli, broiler 7.4%, white semi-broiler 0%, Korean native chicken 18.1% and S. aureus, broiler 38.8%, white semi-broiler 40.0%, Korean native chicken 51.5%.

• Biomedical Science Letters
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• v.18 no.2
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• pp.152-159
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• 2012

Campylobacter species are known to the high optimum growth temperature ($42^{\circ}C$) and the cause of enteritis in people. Erythromycin has a curative effect for enteritis caused by the bacteria. However, the rate of erythromycin-resistant bacteria was not well known until recently in Korea. Swine are one of sources of the infection with a Campylobacter species which cause the symptom of a high temperature. In this study, we cultured rectum fecal specimens of 100 pigs in an area of Buan-gun, Jeonbuk Province during July 2009. As a result, the detection rate of C. jejuni and C. coli and the rate of erythromycin-resistant bacteria for the separated Campylobacter species on the condition of high temperature were investigated. The possession or not of hipO and glyA gene and ciprofloxacin-resistant gene gyrA was also reviewed with biochemical characteristics and PCR.