• Journal of the Korean Wood Science and Technology
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• v.25 no.2
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• pp.45-51
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• 1997

주목 잎을 채취하여 건조하고 아세톤-물 (7 : 3, v/v) 의 혼합액으로 추출한 후 에틸아세테이트 및 수용성 부분으로 분리하고 Sephadex-LH 20으로 충진한 칼럼을 이용하여 2개의 flavan 단량체와 2개의 후라보노이드 배당체를 단리하였다. 에틸아세테이트용성 추출물은 대부분 (+)-catechin 과 (-)-epicatechin으로 구성되어 있었으며 수용성 부분에서는 quercetin-3-0-arabinopyranosyl-($1"'{\rightarrow}6"$)-${\beta}$-D-glucoside와 quercetin-3-O-rutinoside 인 두 개의 탄수화물로 구성된 배당체를 분리하였으며 주목에서는 이들 화합물은 아직 보고된 바가 없다. 이들의 구조결정을 위하여 박층크로마토그래피를 실시하고 $^1H$-NMR과 $^{13}C$-NMR 스펙트럼을 기존의 스펙트럼과 비교, 분석하여 정확한 구조를 규명하였다.

• Natural Product Sciences
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• v.20 no.2
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• pp.119-125
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• 2014

According to the results of my study on the chromatographic analysis of fresh ginseng (Panax ginseng C. A. Meyer) roots, most of the contents of protopanxadiol ginsenosides $Rb_1$, Rc, $Rb_2$, and Rd are derived from the corresponding malonyl ginsenosides in fresh ginseng by a heat process. Also, I confirmed that acetyl ginsenosides are naturally occurring constituents in fresh ginseng, not decarboxylates from malonyl ginsenosides. Seven neutral ginsenosides $Rg_1$, Re, Rf, Rc, $Rb_1$, $Rb_2$, and Rd were transformed to specific conversions in red ginseng preparation conditions. The conversion paths progress by three rules concluded from my study. These conversion rules are I: the ether bond is stable at positions 3 and 6 in the dammarane skeleton, II: the ether bond between sugars is stable in glycosides, and III: the ether bond to glycosides is unstable at position 20 in the dammarane skeleton.

• Archives of Pharmacal Research
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• v.17 no.6
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• pp.462-466
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• 1994

Radical scavenging principles on 1, 1-diphenyl-2-picylhydrazyl(DPPH) radical were isolated from the seeds of Cassis tora L. Assignments of the^1H-{\;}and{\;}^{13}C-NMR$ data showed the active components to be an anthraquinone, alatemin nad two naphthopyrone glycosides, nor-rubro-fusarin-6-.betha.-D-glucoside(cassiaside) and rubrofusarin-6- -D-gentiobioside. Altemin showed more potent radical sacvenging effect than the others.

• Archives of Pharmacal Research
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• v.24 no.4
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• pp.286-291
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• 2001

Seven phenylpropanoid glycosides named acteoside (1), acteoside isomer (2), leucosceptoside A (3), plantainoside C (4), jionoside D (5), martynoside (6), and isomartynoside (7) were isolated from Clerodendron trichotomum. Compounds 1 and 2 showed potent inhibitory activities against HlV-1 integrase with $IC_{20}$ values of 7.8 ${\pm}$ 3.6 and 13.7 ${\pm}$ 6.0${\mu}\textrm{M}$, respectively.

• Applied Biological Chemistry
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• v.51 no.3
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• pp.245-246
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• 2008

• Archives of Pharmacal Research
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• v.10 no.4
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• pp.219-222
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• 1987

From the underground parts of Rosa rugosa(Rosaceae), 28-0-glucosides of euscaphic acid, tormentic acid and arjunic acid were isolated and characterized by spectral data.

• Proceedings of the PSK Conference
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• 2000.10a
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• pp.242.2-242.2
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• 2000

• Journal of the Korean Wood Science and Technology
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• v.43 no.5
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• pp.591-599
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• 2015

Cercidiphyllum japonicum leaves were collected, air-dried and extracted with 70% aqueous acetone, then concentrated and sequentially fractionated using n-hexane, methylene chloride ($CH_2Cl_2$), ethylacetate (EtOAc), and $H_2O$. A portion of EtOAc fraction (10 g) was chromatographed on a Sephadex LH-20 column, by the successively elution with various aqueous $MeOH-H_2O$ (1:9, fraction $1-2{\rightarrow}3:7$, fraction $3-5{\rightarrow}1:1$, fraction $6-9{\rightarrow}7:3$, fraction $10-13{\rightarrow}9:1$, fraction 14-16). Compound 2 was isolated from fraction 6 and compound 1 was separated from fraction 11 and 12. Compound 3 and 4 were purified from fraction 13. The isolated compounds were elucidated as quercetin-3-O-${\alpha}$-L-rhamnopyranoside (1), chlorogenic acid (2), quercetin-3-O-${\alpha}$-L-arabinofuranoside (3) and quercetin-3-O-${\beta}$-D-xylopyranoside (4) by the spectral and literature data, and by comparison with the authentic samples. These compounds were reported, for the first time, from the extracts of C. japonicum leaves. Also chlorogenic acid (2) has never been reported before in domestic tree species and can be used as an index compound for C. japonicum.

• KSBB Journal
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• v.23 no.2
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• pp.131-134
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• 2008

Tea seed extract, a byproduct of tea processing, contains two kaempferol glycosides, camelliaside A and camelliaside B. Kaempferol was produced by enzymatic hydrolysis of glycosides. Optimum reaction conditions were investigated. $Pectinex^{(R)}100L$ was effective, producing kaempferol in 48 hrs. Optimum temperature and pH were $40^{\circ}C$ and 4, respectively. Ratio of substrate and enzyme affected the yield. Under optimum conditions, 1.6g kaempferol per 1 kg tea seed extract was produced and 80% of kaempferol precipitated. This result shows that kaempferol could be produced mildly and effectively using tea-processing byproduct.

• Korean Journal of Pharmacognosy
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• v.42 no.2
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• pp.138-143
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• 2011

A high performance liquid chromatography (HPLC) method was developed for simultaneous determination of two major flavonoid glycosides (poncirin and nanringin) in Poncirus trifoliata Raf. by different harvesting times and locations for two years. A SunFire $C_{18}$ column (4.6 mm${\times}$250 mm, 5 ${\mu}M$) was used at $40^{\circ}C$ for the determination of poncirin and naringin. The mobile phase using gradient flow consisted of two solvent systems. Solvent A was 1.0% (v/v) aqueous acetic acid and solvent B was acetonitrile with 1.0% (v/v) acetic acid. Flow rate was 1.0 mL/min and injection volume was 10 ${\mu}l$. The chromatogram was monitored by photodiode array (PDA) detection at 280 nm for the identification of two flavonoid glycosides in P. trifoliata. The contents of the two components in P. trifoliata ranged from 0.32~13.02%.