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Contents of Poncirin and Naringin in Fruit of Poncirus Trifoliata according to Different Harvesting Times and Locations for Two Years  

Lee, Sul-Lim (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine)
Seo, Chang-Seob (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine)
Kim, Jung-Hoon (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine)
Shin, Hyeun-Kyoo (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine)
Publication Information
Korean Journal of Pharmacognosy / v.42, no.2, 2011 , pp. 138-143 More about this Journal
Abstract
A high performance liquid chromatography (HPLC) method was developed for simultaneous determination of two major flavonoid glycosides (poncirin and nanringin) in Poncirus trifoliata Raf. by different harvesting times and locations for two years. A SunFire $C_{18}$ column (4.6 mm${\times}$250 mm, 5 ${\mu}M$) was used at $40^{\circ}C$ for the determination of poncirin and naringin. The mobile phase using gradient flow consisted of two solvent systems. Solvent A was 1.0% (v/v) aqueous acetic acid and solvent B was acetonitrile with 1.0% (v/v) acetic acid. Flow rate was 1.0 mL/min and injection volume was 10 ${\mu}l$. The chromatogram was monitored by photodiode array (PDA) detection at 280 nm for the identification of two flavonoid glycosides in P. trifoliata. The contents of the two components in P. trifoliata ranged from 0.32~13.02%.
Keywords
Poncirus trifoliata; HPLC; Poncirin; Naringin;
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