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Production of kaempferol by enzymatic hydrolysis of tea seed extract  

Lim, Yun-Young (Department of Biological Engineering, Inha University)
Kim, Eun-Ki (Department of Biological Engineering, Inha University)
Publication Information
KSBB Journal / v.23, no.2, 2008 , pp. 131-134 More about this Journal
Abstract
Tea seed extract, a byproduct of tea processing, contains two kaempferol glycosides, camelliaside A and camelliaside B. Kaempferol was produced by enzymatic hydrolysis of glycosides. Optimum reaction conditions were investigated. $Pectinex^{(R)}100L$ was effective, producing kaempferol in 48 hrs. Optimum temperature and pH were $40^{\circ}C$ and 4, respectively. Ratio of substrate and enzyme affected the yield. Under optimum conditions, 1.6g kaempferol per 1 kg tea seed extract was produced and 80% of kaempferol precipitated. This result shows that kaempferol could be produced mildly and effectively using tea-processing byproduct.
Keywords
Tea Seed Extract; Kaempferol; Enzymatic Hydrolysis;
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