• Animal Bioscience
• /
• v.34 no.8
• /
• pp.1321-1330
• /
• 2021

Objective: Transgenic hens hold a great promise to produce various valuable proteins. Through virus transduction into stage X embryo, the transgene expression under the control of constructed chicken ovalbumin promoters has been successfully achieved. However, a validation system that can evaluate differently developed ovalbumin promoters in in vitro, remains to be developed. Methods: In the present study, chicken oviduct epithelial cells (cOECs) were isolated from oviduct tissue and shortly cultured with keratinocyte complete medium supplemented with chicken serum. The isolated cells were characterized with immunofluorescence, western blot, and flow cytometry using oviduct-specific marker. Chicken mutated ovalbumin promoter (Mut-4.4-kb-pOV) was validated in these cells using luciferase reporter analysis. Results: The isolated cOECs revealed that the oviduct-specific marker, ovalbumin protein, was clearly detected by immunofluorescence, western blot, and flow cytometry analysis revealed that approximately 79.40% of the cells contained this protein. Also, luciferase reporter analysis showed that the constructed Mut-4.4-kb-pOV exhibited 7.1-fold (p<0.001) higher activity in the cOECs. Conclusion: Collectively, these results demonstrate the efficient isolation and characterization of cOECs and validate the activity of the constructed ovalbumin promoter in the cultured cOECs. The in vitro validation of the recombinant promoter activity in cOECs can facilitate the production of efficient transgenic chickens for potential use as bioreactors.

• Journal of Convergence for Information Technology
• /
• v.11 no.1
• /
• pp.12-19
• /
• 2021

For strengthening the national defense, the function of tactical network is essential. tactics and strategies in wartime situations are based on numerous information. Therefore, various reconnaissance devices and resources are used to collect a huge amount of information, and they transmit the information through tactical networks. In tactical networks that which use contention based channel access scheme, high-speed nodes such as recon aircraft may have performance degradation problems due to unnecessary channel occupation. In this paper, we propose a learning-backoff method, which empirically learns the size of the contention window to determine channel access time. The proposed method shows that the network throughput can be increased up to 25% as the number of high-speed mobility nodes are increases.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2021.10a
• /
• pp.603-605
• /
• 2021

With the expansion of the indoor service-providing robot market and the electrification of automobiles, research on autonomous driving is being actively conducted. In general, in the case of outside, the location is mainly recognized through GPS, and location positioning is performed indoors using technologies such as WiFi, UWB (Ultra-Wide Band), VLP, LiDAR, and Vision. In this paper, we introduce a system for location-positioning using LED lights with different color temperatures in an indoor environment. After installing LED lights in a simulated environment such as a tunnel, it was shown that information about the current location can be obtained through the analysis of chromaticity values according to location. Through this, it is expected to be able to obtain information about the location of the vehicle in the tunnel and the movement of the device in a room such as a warehouse or a factory.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2022.09a
• /
• pp.39-39
• /
• 2022

기온, 강수량 등 기후변화에 따른 식물 멸종이 증가함에 따라 기후변화에 민감한 북방계식물의 중요성이 증대되고 있는 실정이다. 본 연구 대상종의 큰금매화(Trollius chinensis)는 북한 및 북방계 식물로 고산 초지에서 자생하는 미나리아재비과 여러해살이풀이며, 기후변화에 직·간접적인 영향을 받고 있는 실정이다. 이에, 큰금매화의 발아조건을 규명하여 종 복원과 대량증식법 개발을 위한 기초자료를 확보하는 데 목적이 있다. 종자의 형태학적 특성은 원형~계란형(circular~obovate)에 작은 돌기가 겹겹이 있는(Colliculate) 형태였으며, 종피는 진갈색이다. 크기는 길이 1.37±0.741mm, 너비 0.81±0.531mm이었다. 종자의 단면을 확인한 결과, 배는 작은 선형태(Axial-miniature)이었으며, 종자는 91%의 충실도를 보였으며 TZ 테스트에서 모두 배가 전체적으로 붉은색으로 염색되어 양호한 활력을 보였다. 처리구별 발아실험 전 종자 소독은 파종 전 70% 에탄올 처리 후 2%의 NaOCl을 넣어 10분 동안 소독 후 멸균수로 5회 수세하고 멸균수를 제거하여 침지처리에 사용하였다. 발아실험 결과 종자의 최종발아율(FGP)은 15/6℃ GA3 1000mg·L^-1 처리에서 40.0%로 가장 높았다. 평균발아일수(MGT)는 15/6℃ 무처리에서 22.8일로 가장 높았으며, 최종발아일의 평균은 약 9.5일로 확인되었다. 대부분 GA3 처리에서 최종발아율(FGP)이 20% 이상 높게 나타났으며, GA3 500mg·L^-1 보다는 GA3 1000mg·L^-1의 조건에서 3.3~5.5% 최종발아율(FGP)이 향상된 것으로 확인되었다. 한편, 온도 조건 25/15℃ 무처리에서는 발아가 확인되지 않았다.

• Journal of Periodontal and Implant Science
• /
• v.42 no.3
• /
• pp.73-80
• /
• 2012

Purpose: The purpose of this study was to investigate the effects of the immunosuppressants FK506 and cyclosporin A (CsA) on the osteogenic differentiation of rat mesenchymal stem cells (MSCs). Methods: The effect of FK506 and CsA on rat MSCs was assessed in vitro. The MTT assay was used to determine the deleterious effect of immunosuppressants on stem cell proliferation at 1, 3, and 7 days. Alkaline phosphatase (ALP) activity was analyzed on days 3, 7, and 14. Alizarin red S staining was done on day 21 to check mineralization nodule formation. Real-time polymerase chain reaction (RT-PCR) was also performed to detect the expressions of bone tissue-specific genes on days 1 and 7. Results: Cell proliferation was promoted more in the FK506 groups than the control or CsA groups on days 3 and 7. The FK506 groups showed increased ALP activity compared to the other groups during the experimental period. The ALP activity of the CsA groups did not differ from the control group in any of the assessments. Mineralization nodule formation was most prominent in the FK506 groups at 21 days. RT-PCR results of the FK506 groups showed that several bone-related genes-osteopontin, osteonectin, and type I collagen (Col-I)-were expressed more than the control in the beginning, but the intensity of expression decreased over time. Runx2 and Dlx5 gene expression were up-regulated on day 7. The effects of 50 nM CsA on osteonectin and Col-I were similar to those of the FK506 groups, but in the 500 nM CsA group, most of the genes were less expressed compared to the control. Conclusions: These results suggest that FK506 enhances the osteoblastic differentiation of rat MSCs. Therefore, FK506 might have a beneficial effect on bone regeneration when immunosuppressants are needed in xenogenic or allogenic stem cell transplantation to treat bone defects.

• The Korean Journal of Malacology
• /
• v.30 no.4
• /
• pp.343-351
• /
• 2014

We determined the effects of neuroactive compounds known as synthetic larval settlement inducers on the settlement of the Pacific oyster C. gigas pediveliger on the larval collector. Six types of the inducers, serotonin (5-HT), ${\gamma}$-amino butyric acid (GABA), L-3,4-dihydroxyphenylalanine (L-DOPA), norepinephrine, epinephrine and methyl bromide (MB) were tested. All the chemicals induced larval settlement, MB being the most effective with settlement rate of $42.7{\pm}2.7%$, followed by GABA ($35.4{\pm}2.0%$), 5-HT ($29.1{\pm}2.2%$), L-DOPA ($19.2{\pm}2.1%$), epinephrine ($15.2{\pm}0.9%$), and norepinephrine ($11.0{\pm}1.2%$). The chemicals ${\gamma}$-amino butyric acid and methyl bromide were also better in terms of settled density on the collector with their respective density of $1.97{\pm}1.42$ and $2.37{\pm}1.86ind/cm^2$, reminiscent of being most effective candidates for a larval settlement inducer in the oyster hatchery.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.22 no.6
• /
• pp.1566-1571
• /
• 2008

Bone is a dynamic tissue that is constantly resorbed by osteoclasts and then replaced by osteoblasts. Osteoclasts, multinucleated cells of monocyte/macrophage lineage, are responsible for bone disorders, including osteoporosis and rheumatoid arthritis. In this study, we examined the effect of the curcumin on osteoclast survival and bone resorption. We found that curcumin significantly inhibited RANKL-mediated osteoclast survival. DAPI stainingrevealed that curcumin induced the apoptotic features of osteoclasts. Although curcumin did not suppress the phosphorylation of Akt and ERK in osteoclasts treated with RANKL, curcumin induced the cleavage of pro-caspase-9 and -3 its active forms. Also, curcumin inhibited the formation of actin rings of osteoclasts. RANKL-mediated bone resorption was inhibited by the addition of curcumin. Together with the results of this study, these findings suggest that the curcumin inhibited the survival of osteoclasts by activating caspase-9 and -3 and suppressed the bone resorptive activity. Thus, curcumin may be developed as antiresorptive drugs for the treatment of bone-related disorders.

• Journal of Korea Spatial Information System Society
• /
• v.6 no.1 s.11
• /
• pp.31-50
• /
• 2004

With the growth of wireless communication networks and mobile devices taking in GPS, Location-Based Service(LBS) is becoming an integral part of mobile applications. LBS can deal with location-aware features such as persons holding mobile phones or vehicles equipped with GPS, and provide the users with the location information of the features. Thus it is necessary to develop moving object database systems to store, manage, and query moving objects which change their locations continuously as time passes. In this paper, we design and implement a query processing component which deals with moving objects as a key data type. For this component, we define a new SQL-like query language(called MOQL) and as a consequence, design and implement modules that analyze and execute queries. It supports various types of operators that process range queries, infer topological relations, compute trajectories, and find k-nearest neighbors. It can be used as a subsystem if other application systems which deal moving objects and also supports ADO.NET interface that can be used to interact end-users.

• Korean Journal of Agricultural and Forest Meteorology
• /
• v.11 no.1
• /
• pp.19-26
• /
• 2009

The standardization of eddy-covariance data processing is essential for the analysis and synthesis of vast amount of data being accumulated through continuous observations in various flux measurement networks. End users eventually benefit from the open and transparent standardization protocol by clear understanding of final products such as evapotranspiration and gross primary productivity. In this paper, we briefly introduced KoFlux efforts to standardize data processing methodologies and then estimated uncertainties of surface fluxes due to different processing methods. Based on our scrutiny of the data observed at Gwangneung KoFlux site, net ecosystem exchange and ecosystem respiration were sensitive to the selection of different processing methods. Gross primary production, however, was consistent within errors due to cancellation of the differences in NEE and Re, emphasizing that independent observation of ecosystem respiration is required for accurate estimates of carbon exchange. Nocturnal soil evaporation was small and thus the annually integrated evapotranspiration was not sensitive to the selection of different data processing methods. The implementation of such standardized data processing protocol to AsiaFlux will enable the establishment of consistent database for validation of models of carbon cycle, dynamic vegetation, and land-atmosphere interaction at regional scale.

• Korean journal of applied entomology
• /
• v.46 no.1 s.145
• /
• pp.19-25
• /
• 2007

A survey to investigate the inhabitation of Callipogon relictus Semenov and evaluate the population of the species in the Gwangneung Forest was conducted during last seven years. During the study, a female was observed in the forest in 2006 which confirmed a possibility of the inhabitation with a small population. Fortunately, a ovipositing female was observed in the forest during this study period in 2006. The female laid more than 20 eggs singly in the crack of bark of the Carpinus laxiflora Blume. We collated the all available data for the species with the present study including the previous reports to provide the information far the conservation plan. It will be needed the long term monitoring and various efforts for the conservation of the species will be need hereafter.