• Asian Pacific Journal of Cancer Prevention
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• v.15 no.3
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• pp.1119-1123
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• 2014

Head and neck cancers (HNC) are extremely complex disease types and it is likely that chromosomal instability is involved in the genetic mechanisms of its genesis. However, there is little information regarding the background levels of chromosome instability in these patients. In this pilot study, we examined spontaneous chromosome instability in short-term lymphocyte cultures (72 hours) from 72 study subjects - 36 newly diagnosed HNC squamous cell carcinoma patients and 36 healthy ethnic controls. We estimated chromosome instability (CIN) using chromosomal aberration (CA) analysis and nuclear level anomalies using the Cytokinesis Block Micronucleus Cytome Assay (CBMN Cyt Assay). The proliferation rates in cultures of peripheral blood lymphocytes (PBL) were assessed by calculating the Cytokinesis Block Proliferation Index (CBPI). Our results showed a significantly higher mean level of spontaneous chromosome type aberrations (CSAs), chromatid type aberration (CTAs) dicentric chromosomes (DIC) and chromosome aneuploidy (CANE UP) in patients (CSAs, $0.0294{\pm}0.0038$; CTAs, $0.0925{\pm}0.0060$; DICs, $0.0213{\pm}0.0003$; and CANE UPs, $0.0308{\pm}0.0035$) compared to controls (CSAs, $0.0005{\pm}0.0003$; CTAs, $0.0058{\pm}0.0015$; DICs, $0.0005{\pm}0.0003$; and CANEUPs, $0.0052{\pm}0.0013$) where p<0.001l. Similarly, spontaneous nuclear anomalies showed significantly higher mean level of micronuclei (MNi), nucleoplasmic bridges (NPBs) and nuclear buds (NBUDs) among cases (MNi, $0.01867{\pm}0.00108$; NPBs, $0.0156{\pm}0.00234$; NBUDs, $0.00658{\pm}0.00068$) compared with controls (MNi, $0.00027{\pm}0.00009$; NPBs, $0.00002{\pm}0.00002$; NBUDs, $0.00011{\pm}0.00007$).The evaluation of CBPI supported genomic instability in the peripheral blood lymphocytes showing a significantly lower proliferation rate in HNC patients ($1.525{\pm}0.005552$) compared to healthy subjects ($1.686{\pm}0.009520$) (p<0.0001). In conclusion, our preliminary results showed that visible spontaneous genomic instability and low rate proliferation in the cultured peripheral lymphocytes of solid tumors could be biomarkers to predict malignancy in early stages.

• Korean Journal of Weed Science
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• v.14 no.4
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• pp.272-279
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• 1994

In order to compare the biological properties of benfuresate and cinosulfuron as herbicides for the chemical control of Eleocharis kuroguwai, some experiments were conducted at greenhouse and field conditions in 1993. Benfuresate and cinosufuron moved to 2cm and 6cm deep in paddy soil and inhibited the growth of E. kuroguwai. Benfuresate was absorbed into the roots of E. kuroguwai. whereas cinosulfuron was absorbed into not only the roots and shoots but also the mesocotyls. After the emergence was inhibited by the soil application of herbicides, the percentage of regrowing numbers was higher at cinosulfuron than at benfuresate, whereas the growth rate was higher at benfuresate on the contrary. Benfuresate brought about complete death of buds as a killing type, but cinosulfuron produced lots of abnormal shoots as an inhibiting type. Benfuresate was able to apply on soil surface from the preemergence to the early stage of 2cm high, but cinosulfuron was able to use from the preemergence to the stage of 15cm high. The foliar application of cinosulfuron showed a good control effect, but it induced a severe phytotoxicity to rice. A good control effect of Benfuresate/bensulfuron GR lowered at the late stage, but that of cinosulfuron/mefenacet/dymron GR at the early stage maintained until the late stage of 65 days after transplanting.

• Journal of Sericultural and Entomological Science
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• v.38 no.2
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• pp.93-99
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• 1996

By treatment(dropping) of 0.1~0.4% colchicine solution on the sprouts of winter buds of hard wood cutting slips for 4~5 days, two lines of artificial autotetraploids from Yongchonppong and one line from Yeongbyonppong were bred and the important cultivative characteristics of those new lines were as follows. The greentip sprouting stage of the new bred lines in spring season is later than the parental varieties by two days, but growth speed of the new lines after sprouting was faster than that of the parental varieties reaching the same level development with the parental varieties at the fifth leaf sprouting stage to be mid varieties same as the origins. The leaf shape of the new bred lines was wide round and the petioloes were long and thick. The thickness of leaf was thicker than the parental varieties by 17-33% and single leaf weight was heavy. The leaf area weight increased by 21-31% and the content of chlorophyll was also higher by 11-33%. With all the characteristics, the new breds produced good quality of leaves. The length and number of branches were shorter and less, respectively, than the parental varieties, but the internode length was either same or longer than the parents. Looking at the characteristics, the constitution of shoots was slightly inferior to the parental varieties. The cold hardness expressed by the death top rate of Sawonppong 23 and Sawonppong 24 was same level as that of Yongchonppong, but Sawonppong 25 was stronger than Yeongbyonppong in it with a high infection rate of dwarf disease. The productivity was lower than the parental varieties, but young shoot rate to shoot and branch and the ratio of leaf to young shoot were higher than the parental varieties. The fertility of Sawonppong 23 and Sawonppong 24 was comparatively high with 62% of cross success, but that of Sawonppong 25 was low with 23.9% of cross success.

• Korean Journal of Plant Resources
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• v.31 no.1
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• pp.71-76
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• 2018

This study was conducted to investigate the effect of shoot twist on fruitfulness and fruit quality of 'Campbell Early' grapevine. Proper pruning and training are essential to produce a good yield of high-quality fruit and to maintain the balance between vegetative growth and fruiting. The most common problem in spur-pruned 'Campbell Early' cultivar is that vigorous buds has low fruitfulness and thereby the shoot become more vigorous the following spring because of lower crop load. Therefore, shoot twists in very vigorous 'Campbell Early' canes (above 10.0 mm) were performed on the third nodes and the $7^{th}$ nodes of each shoot at 7 days before bloom and full bloom, respectively. Sprouting date, blooming date were not significantly different among the treatments while, harvesting date was delayed approximately 3 days. However, number of berries per cluster, cluster weight and fruitfulness were significantly higher in the shoot twist treatment on the third nodes than the control that was topping alone. Combination treatments of shoot twist and topping had an additive effect on increasing cluster weight resulting in higher increase of yield by 12.1 ㎏ per vine. These results indicated that the shoot twist on very vigorous canes of 'Campbell Early' grapevine for well fruitfulness seemed to be very effective.

• Korean Journal of Medicinal Crop Science
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• v.2 no.2
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• pp.154-161
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• 1994

This experiment was carried out to establish micropropagation system in Fritillaria thunbergii Miq. Through the culture of bulblet scales, stems, node-buds and shoot tips with special reference to the effect of physiological age of explant and plant growth regulators on bulblet formation. Number of formed bulblets was significantly increased in node-bud or stem tissue compared to scals segments and on the medium supplemented with kinetin than BA containing medium. Optimum levels of kinetin for bulblet formation from node-bud taken from above 3 cm shoot length and stem segments excised from below 3 cm shoot length were 5.0 mg /L and $1.0{\sim}3.0\;mg$ /L kinetin, respectively. Interesting phenomenon was observed, the direct formation of bulblets from the axilliary bud of cultured explants. Bulblet forming capacity in stem tissue was depended on stem age, young stem had high regeneration ability compared to old stem taken from above 10 cm shoot length. 1.0 mg /L kinetin was optimum concentration for the formation of bulblets from old stem segments. Stem tissue taken from underground growing plant was promoted coampare to shoot tips or bulb scale segments. Optimum concentration of sucrose was $5{\sim}7%$. Summariged above results revealed that effective explant for micropropagation was stem and /or node-bud tissue excised from less than 3 cm plant height compared to those of bulb scale segments which showed high contamination after culture. Maximum multiplication rate of young stem and /or node-bud segment was about 20 times. Kinetin requirement for stimulation of bulblet formation from cultured explant depended on source of explants but favorable levels of kinetin for organogenesis ranged from 1.0 mg /L to 5.0 mg /L.

• Korean Journal of Ichthyology
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• v.17 no.3
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• pp.167-172
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• 2005

The flat-headed goby, Luciogobius guttatus, inhabits tidepools and river mouths, and stays under stones on the dried bottom for the duration of the low tide. To know the relationship of its respiration and habit in this fish, the epidermis of five appendages was observed. The epidermis has three layers: the outermost layer, middle layer and stratum germinativum. The outermost layer is composed of polygonal cells or rather flattened cells, and mucous cells. The unicellular mucous cells showing acid mucopolysaccharides are 11.1 to $16.1{\mu}m$ in mean height and in one or two rows. The middle layer consists mainly of large epidermal cells that are swollen by adjacent epidermal cells and arranged in a web-shaped structure. The swollen cells are 12.3 to $15.2{\mu}m$ in mean height and arranged in one to 11 layers. Since the swollen cells occupy the entire height of the epidermis, the epidermis is thick. A large number of blood capillaries are present just below the stratum germinativum. Taste buds are distributed at intervals on the surface of the epidermis. Based on these epidermal strucures, it is likely that L. guttatus utilizes cutaneous respiration in a dual respiratory systems.

• Journal of the korean academy of Pediatric Dentistry
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• v.41 no.1
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• pp.85-92
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• 2014

Dentigerous cyst is the most common developmental odontogenic cyst of the jaw which is associated with unerupted teeth. Treatment modalities range from enucleation to marsupialization. Enucleation is the process in which the cyst is completely removed, and this is usually indicated for smaller lesions. Larger cysts can be treated by marsupialization, which is a process that consists of making a surgical cavity on the wall of the cyst, emptying its content and maintaining the continuity between the cyst and the oral cavity. This procedure allows decompression of the cyst, regeneration of the bone-defected area, and also lets the wall of the cyst change into normal mucosa. This technique will protect adjacent structures and will promote spontaneous eruption of succedaneous teeth previously surrounded by the cyst. These are two case reports of a 5-year-old and an 11-year-old boy with dentigerous cysts found regarding supernumerary tooth and pulpotomized mandibular primary molars, respectively. Due to the large size of the cysts, both cases were treated with marsupialization. This case report shows that in cases regarding large-sized dentigerous cysts, marsupialization can be an effective surgical technique that promotes spontaneous eruption of cyst-involved succedaneous teeth and the preservation of adjacent teeth.

• Journal of Korean Society of Forest Science
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• v.67 no.1
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• pp.1-9
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• 1984

Taxonomical studies of the genus Acer in Korea (8 species, 3 varieties and 1 forma) were studied by the morphological characteristics of stipules. The results obtained were summarized as follows; 1) The stipules of the genus Acer were arranged oppositely with 4 - 10 stipules consisting of simple leaf, compound leaf and unifoliate compound leaf. The venation of stipules were cladodromous, basal reticulate, fibrous and palmately netted vein. The trichoma of stipules were glandula hair, glandular pubescent, sericeous, pilous, vinous, velutinous, tomentose, ciliate and lanate. As the morphological characteristics of stipules in the genus Acer established a strong point in the identification of species. 2) The species in the genus Acer in Korea were classified into six groups; (1) ginnala group (A. ginnala), (2) koreanum group (A. koreanum, A. japonicum, A. pseudosieboldianum var. koreanum), (3) mono group (A. mono, A, palmatum var. sanguineum, A. palamtum var. dissectum, A. palmatum var. dissectum f. aosidare), (4) negundo group (A. negundo), (5) palmatum group (A. palmatum), (6) platanoides group (A. platanoides, A. truncatum) by the morphological characteristics of stipules, 3) The stipules of platanoides group and negundo group were the least evolved types among the species in the genus Acer. 4) The stipules in the genus Acer were made the inner buds that were metamorphosed to the protected functional aspect by the evolution of petiols.

• Journal of Bio-Environment Control
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• v.26 no.4
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• pp.291-296
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• 2017

This study was conducted to evaluate the influence of early defoliation on photosynthesis and carbohydrate reserves when the source leaves of 'Fuji'/M9 apple (Malus domestica Borkh.) trees were removed during the growing period. Bud regrowth rates of 80%, 50% defoliation and non-defoliation treatments were significantly different 82.7%, 45.9% and 2.1% respectively at 30 days after treatment. In all treatments, sucrose and starch concentrations in remaining leaves decreased non-significantly during the 14-day period. No significant changes were observed for total soluble carbohydrates in non-defoliation and 50% defoliation. However, in 80% defoliated treatments, concentrations of sorbitol and total soluble carbohydrates in remaining leaves declined steadily during the 14-day period. It is thought that high sink strength increases the requirements of carbohydrate from remaining leaves more than non-defoliated. The concentrations of starch in the roots tend to decrease non-significantly as percentage of defoliation increased. Photosynthesis of remaining leaves was monitored during the 14-day period after partial defoliation treatments. Net photosynthetic rates (Pn) and stomatal conductance were significantly enhanced in the 80% defoliation. The observed photosynthetic enhancement following partial defoliation may have been due to the enhancement of osmotic potential in leaves. These results were estimated that increasing of photosynthetic rate in the partial defoliation is due to the sink carbohydrate requirements for the current year's secondary growth of buds.

• Journal of Life Science
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• v.14 no.5
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• pp.809-814
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• 2004

To investigate the effect of the electrolyzed acidic water for soybean sprouts growth, the responses of characteristics of soybean sprouts were evaluated. Soybean sprouts grown by the electrolyzed acidic water showed shorter length in total body, root, and hypocotyl, etc. but they were evaluated to be increased in hypocotyl diameter and weight per sprout. Total length of soybean sprouts grown for 5 days by electrolyzed acidic water were much shorter than those by tap water. Soybean sprouts grown by tap water showed rapid growth in length even after 5 days but no more growth in length for those grown by electrolyzed acidic water. The growth of hypocotyl showed the same tendency as total length. No difference in root length among the soybean sprouts grown for 4 ~ 11 days by electrolyzed acidic water while those grown by tap water showed continuous rapid growth in length. The diameter of hypocotyl was thicker in those grown by electrolyzed acidic water than those grown by tap water and increased up 5 days. The weight of cotyledon grown by electrolyzed acidic water showed the proportional increase to the growing days but those grown by tap water showed no increase in hypocotyl weight up to 7 days, but a little bit increase after 11 days with the growth of new buds. The fresh weight per sprout was higher in those grown by electrolyzed acidic water until 7 days than tap water but it was the same weight in 11 days cultivation. The electrolyzed acidic water effected on shortening of hypocotyl and root length, thickening of hypocotyl diameter, and enlarging of cotyledon during soybean sprout cultivation.