• Journal of Microbiology and Biotechnology
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• v.19 no.5
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• pp.502-510
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• 2009

Although the Escherichia coli heat-labile enterotoxin B subunit (LTB) has already been expressed in several different systems, including prokaryotic and eukaryotic organisms, studies regarding the synthesis of LTB into oligomeric structures of pentameric size in the budding yeast Saccharomyces cerevisiae have been limited. Therefore, this study used a functional signal peptide of the amylase 1A protein from rice to direct the yeast-expressed LTB towards the endoplasmci reticulum to oligomerize with the expected pentameric size. The expression and assembly of the recombinant LTB were confirmed in both the cell-free extract and culture media of the recombinant strain using a Western blot analysis. The binding of the LTB pentamers to intestinal epithelial cell membrane glycolipid receptors was further verified using a GM1-ganglioside enzyme-linked inmmunosorbent assay (GM1-ELISA). On the basis of the GM1-ELISA results, pentameric LTB proteins comprised approximately 0.5-2.0% of the total soluble proteins, and the maximum quantity of secreted LTB was estimated to be 3 mg/l after a 3-day cultivation period. Consequently, the synthesis of LTB monomers and their assembly into biologically active aligomers in a recombinant S. cerevisiae strain demonstrated the feasibility of using a GRAS microorganism-based adjuvant, as well as the development of carriers against mucosal disease.

• Horticultural Science & Technology
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• v.29 no.6
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• pp.539-548
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• 2011

This study was conducted to control unseasonable flowering in a standard chrysanthemum 'Baekma' bred in Korea by 2-chloroethylphosphonic acid (ethephon) and night temperature (NT) through suppression of the transition from a vegetative to a reproductive stage under long day length caused by high NT in summer season. Ethephon was applied either once or twice at a concentration of 0, 200, 400, or $800mg{\cdot}L^{-1}$. The NT within controlled mini-plastic houses was maintained at 13, 17, or $21^{\circ}C$. The NT at $13^{\circ}C$ showed the greatest inhibiting effect of unseasonable flowering among all NTs regardless of various combinations of ethephon concentration and frequency. Moreover, the inhibition tendency of unseasonable flowering was distinctly decreased in a NT-dependant manner. Higher NTs reduced cut flower length and number of leaves, but increased the number of young leaves attached to top part of the flower. Higher ethephon concentrations and lower NTs increased cut flower length and the fresh weight of total, stem, and leaves due to the extension of vegetative growth period. Thus, if it is difficult to control the NT below $21^{\circ}C$ in greenhouses in the summer season, we recommended to spray more than $200mg{\cdot}L^{-1}$ ethephon once after planting to suppress unseasonable flowering and to ensure sufficient length of cut flowers.

• Applied Biological Chemistry
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• v.29 no.4
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• pp.359-365
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• 1986

Exponentially growing cells of S. cerevisiae were cultivated in the concomitant presence of $2{\times}10^{-3}\;M$ paraquat for 12 hours. Cellular growth became slower as the time passed and then, it was completely inhibited after 6 hours of cultivation. More than 70 percent of the yeast cells were killed with in 12 hours of paraquat treatment. It was observed that size of the yeast cell varied from $4.5{\times}5.5{\um}m$ to $2.5{\times}3.5{\um}m$ in diameter, while that of control was uniform. The number of budding cells became rare, Cell wall of the paraquat treated cell was thinner$(0.15{\mu}m)$ than that of control cell$(0.20{\mu}m)$. Outer part of the cell wall had higher density than inner part. Membrane structures such as plasma membrane and mitochondria was decomposed during the paraquat treatment for 12 hours.

• Journal of Sericultural and Entomological Science
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• v.38 no.2
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• pp.81-92
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• 1996

This study was conducted to characterize mulberry varieties by the analysis of morphological characters as well as biochemical and molecular biological markers. As for the budding stage Geumsang, Chosang i and Yeongbyeonchuwoo were early, but Dangsang 6, Hwangchuwoo were late. The lowest varietiy in rate of death atop was Dangsang 8(0.0%). Suncheonppong was the highest leaf yields in spring and autumn rearing season. In biochemical isozyme analysis, peroxidase gave good zymogram patterns in isoelectric focusing electrophoresis. There were high variations in RAPD analysis among the mulberry trees. From the obtained peroxidase and RAPD variations, cluster phylogenetic analysis was carried out using NT-SYS PC program. There were no clear grouping patterns between native varieties and leading varieties. The highest similarity was observed between Suwonsang 1 and Suwonsang 2 at about 90% similarity level.

• Korean Journal of Microbiology
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• v.52 no.4
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• pp.487-494
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• 2016

In Saccharomyces cerevisiae, Paf1 complex consists of five proteins, and they are structurally and functionally well conserved in yeast, fruit fly, plants, and human. With binding to RNA polymerase II from transcription start site to termination site, Paf1 complex functions as a platform for recruiting many types of transcription factors to RNA polymerase II. Paf1 complex contributes to H2B ubiquitination and indirectly influences on H3K4 di- and tri-methylation by histone crosstalk. But the individual effects of five components in Paf1 complex on these two histone modifications including H2B ubiquitination and H3K4 methylation largely remained to be identified. In this study, we constructed the single-gene knockout mutants of each Paf1 complex component and observed H3K4 mono-, di-, and trimethylation as well as H2B ubiquitination in these mutants. Interestingly, in each ${\Delta}paf1$, ${\Delta}rtf1$, and ${\Delta}ctr9$ strain, we observed the dramatic defect in H3K4 monomethylation, which is independent of H2B ubiquitination, as well as H3K4 di- and trimethylation. However, the protein level of Set1, which is methyltransferase for H3K4, was not changed in these mutants. This suggests that Paf1 complex may directly influence on H3K4 methylation by directly regulating the activity of Set1 or the stability of Set1 complex in an H2B ubiquitination independent manner.

• Journal of Sericultural and Entomological Science
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• v.29 no.1
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• pp.1-6
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• 1987

Changes in water content, dry weight, total nitrogen (T-N), P, K, Ca, Mg, B, Fe, Cu, Zn and Mn and death rate were studied in winter buds of mulberry trees. Winter buds of the early season varieties, Hongolbbong and Chongolbbong, (Morus Lhou Koioz) and midseason varieties, Kaeryangbbong and Chongilbbong, (Morus alba L.) were sampled every 7 days from February 28 to April 28, 1986. The result were as follow : 1. Water content was increased by 20% over the level of 45% in late February. The water content in Chongolbbing was the highest, whereas that in the rest of the varieties was nearly equal. 2. Dry weights of winter buds of the early varieties were always greater than those of midseason varieties. The dry weight of buds of early season varieties began to increase in late February and increased rapidly after April 18. There was no weight gain until April 12 in buds of midseason varieties, followed by a slow increase thereafter. 3. T-N and P, K, B, Fe, Cu and Zn concentration increased with time, Mn decreased, and Ca and Mg levels were constant. 4. T-N and Mn levels were higher and P, Ca, Fe and Cu lower in Hongolbbong than in the other varieties. T-N, Mg and Cu levels were lower in Chongolbbong. 5. The death rate of winter buds increased with the approach of the budding season. The rate was highest in Chongolbbong and lowest in Hongolbbong.

• Korean Journal of Microbiology
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• v.37 no.1
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• pp.28-36
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• 2001

These studies were carried out to understand the mechanisms of genes which are related in cell cycle progression at G1/S phase. Mutants involved in cell cycle progression and regulation in Saccharomyces cerevisiae were isolated and characterized. To isolate new mutants, we screened the sensitivity to ciclopirox olamine (CPO) which inhibits the cell cycle traverse at or very near the G1/S phase boundary in HeLa cell and budding yeast. As results, we isolated 30 mutants and named cos(ciclopirox olamine sensitivity: cos27∼cos57) mutants. To determine the phenotype of mutants, we examined the sensitivity to methyl-methane sulfonate (MMS) and hydroxyurea (HU). Several mutants were sensitive to MMS and HU. According to these Phenotypes, cos mutants were grouped into four. Group I mutants are cos27, cos28, cos32, cos33, cos36, cos37, cos40, cos42, cos46, cos50, cos52 and cos53 which show MMS, HU sensitivities and might act at a checkpoint pathway during S phase. Group II mutants are cos43 and cos48 which show MMS sensitivities and might act at a checkpoint pathway during Gl or G2 phase. Group III mutants are cos35, cos47, cos54, cos55 and cos56 which show HU sensitivities and might act at a progress pathway during S phase. Finally, Group IV mutants are cos29, cos30, cos31, cos34, cos38, cos39, cos41, cos44, cos45, cos49, cos51 and cos57 which show only CPO sensitivities. Moreover, we examined the terminal phenotype of mutants under fluorescent microscope and then found one of S phase checkpoint related mutant(cos37). Furthermore, we constructed the heterozygote strain between mutant and wild type haploid strains to study their genetic analysis of cos mutants.

• Journal of Life Science
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• v.26 no.10
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• pp.1137-1152
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• 2016

Cells sense, respond, and adapt to a low oxygen environment called hypoxia, which is widely involved in a variety of human diseases. Adaptation to low oxygen concentrations includes gene expression changes by inducing hypoxic genes and reducing aerobic genes. Recently, the mitochondrial respiratory chain has been implicated in the control of these oxygen-regulated genes when cells experience hypoxia. In order to obtain an insight into an effect of the mitochondrial respiratory chain on cellular response to hyxpoxia, we here examined whole genome transcript signatures of respiration-proficient and respiration-deficient budding yeasts exposed to hypoxia using DNA microarrays. By comparing whole transcriptomes to hypoxia in respiration-proficient and respiration-deficient yeasts, we found that there are several classes of oxygen-regulated genes. Some of them require the mitochondrial respiratory chain for their expression under hypoxia while others do not. We found that the majority of hypoxic genes and aerobic genes need the mitochondrial respiratory chain for their expression under hypoxia. However, we also found that there are some hypoxic and aerobic genes whose expression under hypoxia is independent of the mitochondrial respiratory chain. These results indicate a key involvement of the mitochondrial respiratory chain in oxygen-regulated gene expression and multiple mechanisms for controlling oxygen-regulated gene expression. In addition, we provided gene ontology analyses and computational promoter analyses for hypoxic genes identified in the study. Together with differentially regulated genes under hypoxia, these post-analysis data will be useful resources for understanding the biology of response to hypoxia.

• Journal of the korean academy of Pediatric Dentistry
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• v.33 no.1
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• pp.91-98
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• 2006

Supernumerary teeth are teeth which result from the continued budding of the enamel organ of the preceding tooth or from excessive proliferation of cells. They are most often found in the maxillary anterior region. And they can be responsible for a variey of irregularities in the primary and transitional dentition. There are two morphological types of supernumerary teeth, supplemental and rudimentary. Supplemental teeth have normal shape and size. In contrast, rudimental teeth have abnormal shape and smaller size. Supplemental supernumerary teeth are most common in permanent lateral incisor area. Its extraction must be decided more carefully with differential diagnosis between normal teeth, because it has normal shape and size. We reports 3 cases of the normal incisor shaped teeth in the maxillary anterior region. In all cases, we used the 3D Dental-CT as well as the conventional plain film such as periapical, occlusal, and panoramic radiograph. Consequently, 3B Dental-CT was valualble to figure out the exact position and morphology of supernumerary teeth, to do more conservative surgery and to reduce surgery stress and time.

• Journal of Bio-Environment Control
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• v.20 no.4
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• pp.283-289
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• 2011

This study was conducted to examine the effects of defoliation treatment on the growth and yield of strawberry ($Fragaria{\times}ananassa$ cv. Maehyang and Seolhyang) during nursery period. Leaves of strawberry plantlet had been removed except two, three and four fully expanded leaves until planting date. As the intensity of defoliation was strong, the petiole length was reduced and overgrowth of strawberry plantlet was suppressed. Outer diameter of crown in defoliation treatments significantly decreased but inner diameter of crown was not significant. Number of primary roots of the 3 leaves or 4 leaves defoliation treatment generally tended to increase, but there was not significantly different among treatments. Fresh weight and leaf area in the defoliation treatments significantly decreased and the root weight were higher in partial 3 leaves or 4 leaves defoliation treatment but was not significantly different among treatments. Because T/R ratio decreased significantly as growth inhibition of above-ground part compared to underground part, it is considered easy to take rooting after plantlet plating. As the intensity of defoliation was strong, chlorophyll contents tended to decrease significantly. Reduction of the endogenous nitrogen by defoliation effectively led to promote floral differentiation at low temperature and short day condition. This promoted timing of budding and flowering and also induced uniform flowering after plantlet planting. Marketable fruit yield of 3 leaves defoliation treatment tended to be higher than the control.