• 한국식품영양과학회지
• /
• 제37권11호
• /
• pp.1389-1394
• /
• 2008

본 연구는 산겨릅나무의 식품학적 가치를 알아보고 기능성식품 소재로서의 이용.개발을 위하여 항산화 및 간 기능 보호효과를 탐색하였다. 항산화성 결과 가운데 총 폴리페놀은 EtOAc fr.에서 함량이 가장 많았고 BuOH fr., EtOH ex., $CHCl_3$ fr., hot water ex., aqueous fr. 순으로 높게 나타났다. 전자공여능은 EtOAc fr.과 BuOH fr.이 ascorbic acid와 유사한 전자공여능을 보였으며 SOD 유사활성능은 $250\;{\mu}g/mL$ 이하의 농도에서는 BuOH fr.이 가장 높게, 그 이상에서는 aqueous fr.에서 가장 높게 나타났다. LPS로 급성 간 손상을 유도한 마우스에서의 BuOH fr.의 간 기능 보호효과에서는 BuOH fr.을 전 처치한 다음 LPS를 투여한 군이 LPS만을 투여한 군보다 혈장 AST와 ALT의 활성은 유의하게 감소 되었으나 BUN의 농도는 유의성이 관찰되지 않았다. 간조직의 형태학적 관찰에서 BuOH fr.을 투여하고 LPS로 급성 간 손상을 유도한 군의 간세포는 염증과 괴사가 나타났지만 LPS군에 비하여 그 정도가 적은 것으로 나타났다.

• 대한한의학회지
• /
• 제32권1호
• /
• pp.141-150
• /
• 2011

Objectives: The aim of this study was to elucidate the antioxidant effect of solvent fractions of Angelica gigas root. Methods: The ethanol extract of Angelica gigas root was suspended in water and then partitioned with dichloromethane (MC Fr.), ethyl acetate (EA Fr.) and butanol (BuOH Fr.), sequentially. The antioxidant activities of solvent fractions of Angelica gigas root were evaluated for radical scavenging activity against stable free radicals (2,2-diphenyl-1-picrylhydrazyl) DPPH, hydrogen peroxide and superoxide anion radicals. In addition the antioxidant activities of solvent fractions of Angelica gigas root against peroxyl radicals, hydroxyl radicals and peroxynitrites were determined by the total oxy-radical scavenging capacity (TOSC) assay. Results: Among the solvent fractions of MC Fr., EA Fr., and BuOH Fr., BuOH Fr. was found to have stronger antioxidant activity with $IC_{50}$ values of 59.72, 14.36, 30.96 and $44.75\;{\mu}g/m{\ell}$ on the DPPH radical, nitrite, superoxide anion and hydrogen peroxide, respectively, than BHA used as a positive control. Moreover, specific TOSC values(564.8, 276.4 and 405.5 TOSC/mM) of BuOH fr. against peroxyl radicals, hydroxyl radical and peroxynitrite were 4 times higher than GSH (136.5, 67.4 102.6 TOSC/mM) used as a positive control. Conclusions: These results suggest that the BuOH fr. of Angelica gigas root has a high antioxidant activity and can be useful to develop functional food against oxidative stress conditions.

• 대한본초학회지
• /
• 제27권3호
• /
• pp.23-29
• /
• 2012

Objective : Sanguisorbae Radix(SO). that belong to Rosaceae is widely distributed in Asia including Korea, Japan and China. It has been used as traditional medicine from old times as a treatment for anti-inflammatory drugs. This study was designed to investigate the effects of n-BuOH fractions of SO on anti-oxidant effect and anti-microbial effect on $Streptococcus$ $mutans$ ($S.$ $mutans$). Methods : The anti-microbial effect of $n$-BuOH fractions of SO. was assessed by the paper disk diffusion method and anti-oxidant effect was assessed by the DPPH radical scavenging effect, Superoxide anion radical scavenging effect and SOD like ability. Results : DPPH radical scavenging of the $n$-BuOH fraction from SO in 50 ug/mL was shown to be Fr-2 (89.9%), Fr-3 (88.7%), Fr-4 (76.3%), Fr-1 (59.4%), Fr-5 (56.2%). Superoxside anion radical scavenging activity of the n-BuOH fraction from SO. in 50 ug/mL for Fr-3 was shown to be 78%. SOD-like activity of n-BuOH fraction from the SO in 1000 ug/mL for Fr-3 were shown to be 76.1% respectively. The $n$-BuOH fraction from the SO had high anti-microbial effect on $S.$ $mutans$. Conclusion : As a result, the $n$-BuOH fraction from SO. has good anti-microbial and anti-oxidant effects in a concentration-dependent manner.

• 생약학회지
• /
• 제26권1호
• /
• pp.74-77
• /
• 1995

Several coumarins isolated from Angelica sp. were described to show inhibitory effects against human platelet aggregation. The anti-thrombotic and anti-platelet potential was evaluated, in this paper, with the BuOH soluble fraction of Angelica dahurica root. The BuOH fraction was divided into five subfractions fr. A - E and tested in the mouse model of thrombosis. Survival was enhanced to 35% with fr. A or fr. E treated (500 mg/Kg, p.o.) group of mice compared with 5% survival of the control group. However, none of the 8 coumarin glycosides obtained from fr. A, at the conc. of 0.5 mg/ml, showed inhibitory effects against rat platelet aggregation induced by ADP or collagen.

• 생약학회지
• /
• 제26권4호
• /
• pp.385-389
• /
• 1995

MeOH extract of Gastrodia elata was fractionated to five solvent fractions, hexane fr (fr I), 90% MeOH fr (fr II), EtOAc fr (fr III), BuOH fr (fr IV) and $H_2O$ fr (fr V). Among the five fractions, fr II, III and IV showed platelet anti-aggregating effects against ADP or collagen induced rat platelet aggregation in vitro. Fr II , III and IV were also tested in vivo, in the mouse and rat models of thrombosis. Oral administration of fr II, III or IV enhanced the recovery from the thrombotic shock in the mouse model of thrombosis to 32-40% from 17% of recovery with the control group of mice. Treatment with fr II, III or IV also attenuated the sudden reduction in the blood platelet count following intravenous collagen injection to rat. The above results were indicative of the presence of anti-platelet and anti-thrombotic components in this plant.

• Biomolecules & Therapeutics
• /
• 제4권4호
• /
• pp.437-442
• /
• 1996

Hypoglycemic effect of Tabebuia avellandae was investigated in the streptozotocin(STZ)-induced diabetic rats. Diabetes was induced in male Sprague-Dawley rats by injections of STZ (45 mg/kg, i.v.). Rats weighing 200-250 g were divided into 6 groups: normal, STZ-control, hexane fr., CHCl$_3$fr., BuOH fr. and $H_2O$ fr. group. Normal and STZ-control rats received 3% Tween 80 only. Four groups of diabetic rats were administered orally at doses of 100, 400, 300 and 400 mg/kg/day of hexane, CHC1$_3$, BuOH and $H_2O$ fr. respectively. Fractions were administered orally to the rats for 7 days after STZ injection. All rats were anesthetized with ether, blood samples were taken by cardiac puncture for clinical chemistry and the rats were killed by exsanguination. Liver, kidney, heart and spleen were removed, weighed and analyzed. We measured glucose, protein, cholesterol and triglyceride levels in the plasma and glycogen, cholesterol and triglyceride levels in liver. The extent of blood glucose decrement in rats administered $H_2O$ fraction was greater than that in the STZ-control rats. The serum cholesterol and triglyceride levels were significantly lowered by administration of $H_2O$ fraction compared with those of STZ-control group. Treatment of rats with Tabebuia avellandae fractions caused decreases in STZ-induced elevation of cholesterol and triglyceride. Liver triglyceride level was significantly lowered hexane and BuOH fraction group compared with STZ-control group. These results suggest that $H_2O$ fraction of Tabebuia avellandae has the hypoglycemic action against STZ-induced diabetic rats.

• 한국미생물·생명공학회지
• /
• 제38권4호
• /
• pp.434-441
• /
• 2010

최근 연구에서 폴리페놀을 함유한 천연물은 질병의 위험과 노화의 원인인 ROS와 RNS를 감소시키는 것과 연관이 있다고 밝혔다. 본 연구에서는 섬쑥부쟁이 추출물과 분획물의 산화와 염증에 대한 저해 효과가 있는지에 대한 연구를 조사하였다. 섬쑥부쟁이 70% 메탄올 추출물은 헥산층, 에틸아세테이트층, 부탄올층, 물층을 극성별로 분획하였다. 섬쑥부쟁이로부터 분획한 에틸아세테이트 추출물과 부탄올 추출물의 전자공여능은 $50\;{\mu}g/mL$에서 각각 58.0%, 46.4%를 나타냈다. 에틸아세테이트 추출물의 superoxide anion radical 소거능은 $50\;{\mu}g/mL$에서 64.65%를 나타냈고, 부탄올 추출물은 $50\;{\mu}g/mL$의 농도에서 35.66%의 저해 효과를 나타내었다. 항염증 효과와 관련된 hyaluronidase와 lipoxygenase의 저해 활성에 대한 에틸아세테이트 분획물의 효과는 $5\;{\mu}g/mL$의 농도에서 각각 24.37%, 29.5%의 저해 효과를 나타냈다. 이러한 에틸아세테이트 추출물의 항염증 효과는 nitric oxide 생성 억제에서도 유의했으며, Raw 264.7 cell 내 LPS에 의해 유도된 iNOS 단백질에서 $50\;{\mu}g/mL$의 EtOAc 추출물을 처리시 81.5%의 발현을 억제하는 효과가 확인되었다.

• 동아시아식생활학회지
• /
• 제9권4호
• /
• pp.442-451
• /
• 1999

본 연구는 매실을 천연 항균물질로서의 이용 가능성을 검색하였다. 먼저 매실을 물과 methanol로 추출한 extracts를 paper disc법으로 항균활성을 확인한 후 MeOH extract를 용매별로 분획한 각각의 fractions으로 paper disc법과 그 결과를 토대로 활성이 높은 fractions을 비탁법을 이용하여 농도별로 액체 배지상에서 저해활성을 확인하였다. 변패균인 M leteus는 매실의 EtOAc 획분과 BuOH 획분에 의해서 저해되었고 또한 $H_2O$ 획분과 CHCㅣ$_3$ 획분도 저해활성을 보였다. 한편 저해농도 시템에서는 BuOH 획분 100ppm 첨가 시 전혀 생육을 하지 못했다. 매실의 EtOAc 획분과 BuOH 획분은 B. cereus, B. subtilis와 S. epidrimidis에 대해서 강한 저해 활성을 보였다. 저해농도 시험에서는 B. cereus는 BuOH 획분 100ppm, cB. subtilis는 EtOAc 획분 100ppm, S. epidrimidis는 EtOAc 획분 100ppm에서 생육을 하지 못했다. G(-)세균은 매실성분에 의해서 저해를 받지만 G(+)세균에 비하여 저항성이 켰다. E. coli는 EtOAc 획분 500ppm에서 미약한 生育을 하였으며 S. typhimurium는 EtOAc 획분, BuOH 획분 모두 1,000ppm에서 생육을 전혀 하지 못했으며 P. vulgaris는 BuOH 획분 100ppm, V. parahaemolyticus는 BuOH 획분, EtOAc 획분 100ppm에서 전혀 생육을 하지 못하는 결과를 얻었다. 한편, 효모중 S. cerevisiae IF01950은 EtOAc 획분 500ppm에서 50%의 저해를 받았으며 S. cerevisiae ATCC4105는 1,000ppm의 EtOAc fr. 에서 50%의 저해를 하였다. 항곰팡이 실험에서는 A. niger에서만이 아주 미약한 저해를 보였다.

• 한국조리학회:학술대회논문집
• /
• 한국조리학회 2001년도 하계정기학술세미나
• /
• pp.29-41
• /
• 2001

본 연구는 매실을 천연 항균물질로서의 이용 가능성을 검색하였다. 먼저 매실을 물과 methanol로 추출 한 extracts를 paper disc법으로 항균활성을 확인한 후 MeOH extract를 용매별로 분획한 각각의 fractions으로 paper disc법과 그 결과를 토대로 활성이 높은 fractions을 비탁법을 이용하여 농도별로 액체 배지 상에서 저해활성을 확인하였다. 변패균인 M. leteus는 매실의 EtOAc 획분과 BuOH 획분에 의해서 저해 되었고 또한 $H_2O$ 획분과 CHCl$_3$ 획분도 저해활성을 보였다. 한편 저해농도 시험에서는 BuOH 획분 100ppm 첨가 시 전혀 생육을 하지 못했다. 매실의 EtOAc 획분과 BuOH 획분은 B. cereus, B. subtilis와 S. epidrimidis에 대해서 강한 저해 활성을 보였다. 저해농도 시험에서는 B. cereus는 BuOH 획분 100ppm, B. subtilis는 EtOAc 획분 100ppm, S. epidrimidis는 EtOAc 획분 100ppm에서생육을 하지 못했다. G(-)세균은 매실성분에 의해서 저해를 받지만 G(+)세균에 비하여 저항성이 컸다. E. coli는 EtOAc 획분 500ppm에서 미약한 생육을 하엿으며 S. typhimurium는EtOAC 획분, BuOH 획분 모두 1,000ppm에서 생육을 전혀 하지 못했으며 P. vulgaris는 BuOH 획분 100ppm, V. parahaemolyticus는 BuOH 획분, EtOAc 획분 100ppm에서 전혀 생육을 하지 못하는 결과를 얻었다 한편, 효모중 S. cerevisiae IF1950은 EtOAc 획분 500ppm에서 50%의 저해를 받았으며 S. cerevisiae ATCC4105는 1,000pm의 EtOAc fr. 에서 50%의 저해를 하였다. 항곰팡이 실험에서는 A. niger에서만이 아주 미약한 저해를 보였다.

• Archives of Pharmacal Research
• /
• 제26권11호
• /
• pp.906-911
• /
• 2003

We previously reported that the butanol (BuOH) fraction of the head of Panax ginseng exhibited gastroprotective activity in peptic and chronic ulcer models. In order to identify the active constituent, an activity-guided isolation of the BuOH faction was conducted with a HCI$.$ethanol-induced gastric lesion model. The BuOH fraction was passed through a silica-gel column using a chloroform-methanol gradient solvent system, and six fractions (frs. 1-6) were obtained. The active fr. 5 was further separated by silica-gel column, to yield 6 subfractions (subfrs. a-f). Subfr. d was composed of ginsenosides Re, Rc and $Rb_1$. The most active constituent was ginsenoside $Rb_1$ ($GRb_1$), a protopanaxadiol glycoside, which was investigated for its anti-ulcer effect. Gastric injury induced by HCI$.$ethanol, indomethacin and pyloric ligation (Shay ulcer) was apparently reduced with oral $GRb_1$ doses of 150 and 300 mg/kg. $GRb_1$ at these dosage significantly increased the amount of mucus secretion in an ethanol-induced model. The anti-ulcer effects were consistent with the result of histological examination. These results suggest that the major active constituent in the head of Panax ginseng is $GRb_1$ and that anti-ulcer effect is produced through an increase in mucus secretion.