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http://dx.doi.org/10.6116/kjh.2012.27.3.23

Anti-microbial Effect on Streptococcus mutans and Anti-oxidant Effect of the Butanol Fractions of Sanguisorbae Radix  

Park, Geun-Hye (Department of Cosmeceutical Science, Daegu Haany University)
Lee, Jin-Tae (Department of Cosmeceutical Science, Daegu Haany University)
An, Bong-Jeun (Department of Cosmeceutical Science, Daegu Haany University)
Publication Information
The Korea Journal of Herbology / v.27, no.3, 2012 , pp. 23-29 More about this Journal
Abstract
Objective : Sanguisorbae Radix(SO). that belong to Rosaceae is widely distributed in Asia including Korea, Japan and China. It has been used as traditional medicine from old times as a treatment for anti-inflammatory drugs. This study was designed to investigate the effects of n-BuOH fractions of SO on anti-oxidant effect and anti-microbial effect on $Streptococcus$ $mutans$ ($S.$ $mutans$). Methods : The anti-microbial effect of $n$-BuOH fractions of SO. was assessed by the paper disk diffusion method and anti-oxidant effect was assessed by the DPPH radical scavenging effect, Superoxide anion radical scavenging effect and SOD like ability. Results : DPPH radical scavenging of the $n$-BuOH fraction from SO in 50 ug/mL was shown to be Fr-2 (89.9%), Fr-3 (88.7%), Fr-4 (76.3%), Fr-1 (59.4%), Fr-5 (56.2%). Superoxside anion radical scavenging activity of the n-BuOH fraction from SO. in 50 ug/mL for Fr-3 was shown to be 78%. SOD-like activity of n-BuOH fraction from the SO in 1000 ug/mL for Fr-3 were shown to be 76.1% respectively. The $n$-BuOH fraction from the SO had high anti-microbial effect on $S.$ $mutans$. Conclusion : As a result, the $n$-BuOH fraction from SO. has good anti-microbial and anti-oxidant effects in a concentration-dependent manner.
Keywords
Sanguisorbae Radix; anti-oxidant; anti-microbial;
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1 Lee SL, Kim JG. Anti-microbial activity of soybean extract against oral microbes. Kor J Env Hlth. 2006 ; 32(2) : 192-197.   과학기술학회마을
2 Ferreira, LF and Reid MB. Muscle-derived ROS and thiol regulation in muscle fatigue. J Appl physiol. 2008 ; 104(3) : 853-860.   DOI   ScienceOn
3 Freeman BA and Crapo JD. Biology of disease, free radicals and tissue injury. Lab Inves. 1982 ; 47(5) : 412-426.
4 Wierzba TH, Olek RA, Fedeli D and Falcioni G. Lymphocyte DNA damage in rats challenged with a single bout of strenuous exercise. J Physiol Pharmacol. 2006 ; 57(10) : 115-131.
5 Lloyd, D. Microbial ecology. How to avoid oxygen. Science. 1999 ; 286(5438) : 249.   DOI   ScienceOn
6 Stirpe F and Della Corte E. The regulation of rat liver xanthine oxidase. Conversion in vitro of the enzyme activity from dehydrogenase (type D) to oxidase (type O). J Biol Chem. 1969 ; 244(14) : 3855-3863.
7 Marklund S and Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974 ; 47(3) : 469-474.   DOI   ScienceOn
8 Miyoshi M, Imoto T, Kasagi T. Antieurodontic effect of various fraction extracted from the leaves of Gymnema sylvestre. J Yonago Med Ass. 1987 ; 37 : 127-137.
9 Davidson PM and Parish ME. Method for testing the efficacy of food antimicrobials. Food Technol., 1989 ; 43 : 148-155.
10 Okamura H, Mimura A, Yakou Y, Niwano M and Takahashi Y. Antioxidant activity of tannins and flavonoids in Eucalyptus rostrata. phytochemistry. 1993 ; 33(3) : 557-561.   DOI   ScienceOn
11 Kwon IB, Lee YW, An BJ, and Lee SY. Inhibitory effect of Cacao bean husk extract on Glicosyl transferase from Streptococus mutans B13. Korean J Biotechnol Bioeng. 1993 ; 8 : 75-82.
12 Seo BI, Jung KY. The comprehensible herbology. Daegu Haany University Press . 2004 : 267.
13 Kumar S., Kuma D., Manjusha., Saroha K., Singh N., Vashishta B. Antioxidant and free radical scavenging potential of Citrullus colocynthis (L.) Schrad. methanolic fruit extract. Acta Pharm. 2008 ; 58 : 215-220.   DOI   ScienceOn
14 An BJ, Lee JT, Lee SA, Kwak JH, Park JM, Lee JY, son JH. Antioxidant effect and application as natural ingredients of Korean Sanguisorbae officinalis L. J Korean soc Appl Biol Chem. 2004 ; 47(2) : 244-250.
15 Lim JP. Herb biochemistry. Sinil books. 2003 : 247.
16 Son KJ, Lee SA, Lee G D, Kim YS, Jeon JG, Chang KY. Effects of crude Sanguisorbaofficinalis L. extract on the growth and the adherence to hydroxyapatite beads of mutans streptococci. J Korean acad dent health. 2004 ; 28(1) : 97-104.
17 Kang BS, Ko UC, Kim SH, No SH, Shin YB, Song HJ, Shin MG, Ann DG, Lee SI, Lee YJ, Lee TH, and Ju YS. Collaboration with a national college of oriental medicine herbology professor association. In Herbology. Yonglim Press, Seoul, Korea. 1994 : 392-393.
18 Blois MS. Antioxidant determination by the use of a stable free radical. Nature. 1958 ; 26 : 1199-1120.
19 Park HS, Min KJ, Cha CG, song JW, son JC. Antimicrobial activities against oral microbes and growth-inhibitory effect on oral tumor cell by extract of Paeonia lactiflora. Kor J Env Hlth. 2007 ; 33(1) : 21-29.   과학기술학회마을   DOI   ScienceOn
20 Aruoma OI. Free radical, oxidative stress, and antioxidants in human health and disease. J Am Oil Chem soc. 1998 ; 75 : 199-212.   DOI   ScienceOn
21 Wojcik M, Burzynska-Pedziwiatr I, Wozniak LA. A review of natural and synthetic antioxidants important for health and longevity. Curr Med Chem. 2010 ; 17(28) : 3262-3288.   DOI   ScienceOn
22 Irshad M, Chaudhuri PS. Oxidant-antioxidant system： role and significance in human body. Indian J Exp Biol. 2002 ; 40(11) : 1233-1239.
23 Sies H. Glutathione and its role in cellular functions. Free Radic Biol Med 1999 ; 27 : 916-921.   DOI   ScienceOn
24 Yuan L, Kaplowitz N. Glutathione in liver diseases and hepatotoxicity. Mol Aspects Med. 2009 ; 30(1-2) : 29-41.   DOI   ScienceOn
25 Cesaratto L, Vascotto C, Calligaris S, Tell G. The importance of redox state in liver damage. Ann Hepatol. 2004 ; 3(3) : 86-92.
26 Geronikaki AA, Gavalas AM. Antioxidants and inflammatory disease： synthetic and natural antioxidants with anti-inflammatory activity. Comb Chem High Throughput Screen. 2006 ; 9 : 425-442.   DOI