• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.9
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• pp.4038-4045
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• 2011

To investigate whitening Effects of Angelica dahurica Radix Ethanol Extract (ADEE), we used melan-a cell line, brown guinea pig, and HMB-45. We treated with ADEE of 6.25, 12.5, 25, and 50 ${\mu}g/m{\ell}$ concentration in order to evaluate the effect of ADEE on cell viability and on morphological observation of melan-a cells. Also we were induced the artificial tanning spots by 1,500 mJ/$cm^2$ of ultraviolet B radiation on the backs of brown guinea pigs (approximately 450~500g) and then the test agent of $30{\mu}{\ell}$ was applied on the spots twice a day, five days a week, for five weeks respectively. The visible whitening effect was evaluated once a week. At the end of the experiment, the animals were sacrificed under anesthetization. The artificial tanning spots were obtained by biopsy punch and stained with HMB-45 to observe the gp100 proteins which were melanosomes. Our results show that cell viability was not reduce at ADEE concentrations between 6.25 and 50 ${\mu}g/m{\ell}$, melanin synthesis and melanocyte dendricity were decreased in ADEE treated melan-a cells increasing ADEE concentration. In the gross observation, ADEE treated groups had lower pigmentation than the vehicle control groups. And in the histological observation, ADEE treated groups had lower melanocytes than the vehicle control groups. Also in the quantitative analysis of the gp100 proteins using image analysis software, ADEE treated groups had a significantly lower value (p<0.001) than the vehicle control group and this resultsagreed with the results of observation under microscope. From these results, weconcluded that ADEE had positive whitening effect.

• Archives of Pharmacal Research
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• v.28 no.6
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• pp.691-698
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• 2005

Phlorotannins, the polyphonic compounds found in brown Eisenia and Ecklonia algae, have several pharmacologically beneficial effects such as anti-inflammation. In addition, our recent data show that these compounds may improve the cognitive functions of aged humans suggesting the potential ability to enhance memory in several neurodegenerative disorders. To examine the experimental hypothesis that two effective components of Ecklonia cava, dieckol and phlorofucofuroeckol (PFF), have memory-enhancing abilities, both were administered orally to mice before a passive avoidance test. The repeated administration of either dieckol or PFF dose-dependently reduced the inhibition of latency by the administration of ethanol. To investigate the mode of memory-enhancing actions, the levels of major central neurotransmitters in three different regions (striatum, hippocampus, and frontal cortex) of the mouse brain were measured. The levels of some of the neurotransmitters were significantly changed by ethanol. Both dieckol and PFF altered the levels of some neurotransmitters modified by the ethanol treatment. It is noteworthy that both dieckol and PFF increased the level of acetylcho-line, and they exerted anticholinesterase activities. Overall, the memory-enhancing abilities of dieckol and PFF may result from, at least in part, the increment of the brain level of acetylcho-line by inhibiting acetylcholinesterase.

• BMB Reports
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• v.43 no.1
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• pp.62-68
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• 2010

Matrix Metalloproteinases (MMPs) are a family of zinc-endopeptidases which can degrade extracellular matrix (ECM) components and play important roles in a variety of biological and pathological processes. 6,6'-bieckol isolated and characterized from an edible marine brown alga Ecklonia cava (EC), according to the comprehensive spectral analysis of MS and NMR data. Here the influence of 6,6'-bieckol on expressions of MMPs was examined by zymography and western blot analysis via human fibrosarcoma cell line (HT1080). It is shown that 6,6'-bieckol significantly down regulated the expressions of MMP-2 and -9 in dose-dependent manner. The influence of 6,6'-bieckol on the cell viability and cell behavior of HT1080 cells were also investigated, our dates shown that it suppressed the migration and 3D culture in HT1080 cells. Meanwhile, we explored several signal pathways which may contribute to this process, and found the suppressing of MMPs expressions in HT1080 cells might be due to the suppression of NF-${\kappa}B$ signal pathway.

• Environmental Analysis Health and Toxicology
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• v.8 no.3_4
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• pp.7-12
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• 1993

An Actinomycete strain isolated from Mt. Dea-Dun had a strong antifungal activity. The culture brith produced by isolated strain showed only antifungal activity against fungi with the exception of yeast and bacteria. It was heat stable, dissolved in ehtylacetate. The concentrated antifungal agent showed cytotoxicity against HEP-2 and HeLa as tumor cell line, and showed weak cytotoxicity against VERO 36 as normal cell line. Morphological and physiological characteristics were tested with isolated strain. The spore color of isolated strain was gray. It had a short chain and produced brown colored lytic substance in yeast extract-malt agar. The cell wall of isolated strain was composed of meso-DAP, and we suggested it as genus Actinomadura. In the existing of chemical inhibitor, isolated strain grew on the condition of 0.0001% crystal violet, 0.1% phenol, 0.01% sodium azide and 10% sodium chloride. Carbon utilization of isolated strain was shown that glucose, sucrose, manitol and sodium citrate were well utilized.

• Korean Journal of Poultry Science
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• v.17 no.4
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• pp.243-254
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• 1990

This study was carried out to investigate the effectiveness of the selection for commercial populations of layer chickens subjected to multiple objectives. For that purpose, predicted selection responses obtained from estimated genetic parameters and observed values were compared. Data for two layer pure lines, Line-w(Single Comb White Leghorn) and Line-B (brown layer) which have been maintained at the Mani Breeding Farm were collected from 1980 to 1985 during 5 generations. The annual genetic gains were estimated by the moth of selection index in retrospect on the basis of selection differentials, phenotypic and genetic variance-covariance for the traits considered. SM was found to be shorten by 1.67 days and 1.14 days per generation in Line-W and Line-B. EN300 and EN400 were found to be increased by 4.15 and 5.92 per generation in Line-W and 257 and 3.73 per generation in Line-B. The annual gains realized were found to be significantly different from expected gains predicted on the basis of the annual selection differentials and genetic parameters in later generations, but it could not be concluded that the differences were attributed to genetic effects.

• Korean Journal of Ichthyology
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• v.22 no.2
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• pp.126-128
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• 2010

Morphological characters of Heteropriacanthus cruentatus are redescribed in detail based on a single specimen (179.3 mm SL) collected from the coastal waters of Jeju Island, Korea. The species is characterized by dark brown spots scattered on the caudal fin and the soft-rayed portions of the dorsal and anal fins, and 59 pored lateral line scales.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2002.11a
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• pp.118-119
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• 2002

This experiment was conduct to determine the influences of different levels of dietary energy and crude protein on growth performances during growing period (2 to 10 wk of age). A total of 360 egg breeder chicks (Hy-Line Brown) was divided into 5 groups with 4 replicates of 18 birds per replicate and fed the experimental diets containing three levels of energy and three levels of protein. With the increase in dietary crude protein levels, body weight significantly increased (P〈0.05). But dietary energy levels did not greatly affect growth performances. There were no significant differences to flock uniformity and tibia strength among treatments.

• Journal of Environmental Science International
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• v.27 no.5
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• pp.341-344
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• 2018

This study was conducted to determine the effects of dietary bentonite supplementation on the fatty acid profiles of eggs in older laying hens. A total of 90 Hy-line Brown layers, 74 weeks of age, were confined in 6 wire cages and then assigned randomly to two groups to receive one of the two diets (3 replicates of 15 older layers each) containing 0 and 0.2% bentonite for 4 weeks. After the 4-week feeding trial, no remarkable differences in individual fatty acid, saturated fatty acid (SFA), unsaturated fatty acid (UFA), and mono unsaturated fatty acid (MUFA) levels were found. However, linoleic acid, docosahexaenoic acid, and poly unsaturated fatty acid (PUFA) and UFA/SFA ratio were influenced by 0.2% bentonite. In conclusion, supplementation with 0.2% bentonite improved the profiles of PUFA more than those of SFA, UFA, and MUFA inthe eggs of the older laying hens.

• The Plant Pathology Journal
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• v.23 no.3
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• pp.212-214
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• 2007

In 2005 and 2007, a basal stem rot of Disporum smilacinum caused by Sclerotium rolfsii occurred sporadically in a herb farm at Hamyang, Korea. The symptom initiated with water-soaking lesion and progressed into stem rot and wilt of a whole plant. Severely infected plants were blighted and died eventually. White mycelial mats appeared on the lesion at early stage and a number of sclerotia were formed on the stem near the soil line. The sclerotia were globoid in shape, 1-3 mm in size and white to brown in color. The optimum temperature for the growth and sclerotia formation was 30 on PDA and the hyphal width was measured $3-8{\mu}m$. The typical clamp connections were observed in the hyphae of the fungus grown on PDA. On the basis of symptom, mycological characteristics and pathogenicity to the host plant, this fungus was identified as Sclerotium rolfsii Saccardo. This is the first report on the stem rot of D. smilacinum caused by S. rolfsii in Korea.

• Fisheries and Aquatic Sciences
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• v.10 no.2
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• pp.68-73
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• 2007

Based on four juvenile specimens of Lutjanus collected from the southern coast of Jeju Island, three species, L. argentimaculatus, L. fulviflamma, and L. quinquelineatus, are described as the first record from Korea. The first species is easily differentiated from other lutjanid juveniles by distinct coloring of the dorsal, pelvic, and anal fins as well as two blue lines below the eye; the second species is characterized by having six yellowish brown stripes below the lateral line and a black spot on the side; the third species is identified by five longitudinal blue stripes and a black spot on the side when fresh. The new Korean names "Bul-geun-tung-dom," "Yuk-seon-jeomtung-dom," and "O-seon-tung-dom" are proposed for L. argentimaculatus, L. fulviflamma, and L. quinquelineatus, respectively.