Environmental Analysis Health and Toxicology
- Volume 8 Issue 3_4
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- Pages.7-12
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- 1993
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- 2671-9525(eISSN)
Study of Cytotoxicity of an Actinomycete Isolated in Korea
토양에서 분리한 방선균의 세포 독성에 관한 연구
- Park, Joon-Koo (Department of Microbiology, College of Natural Science,Dankook University) ;
- Choi, Boung-Don (Department of Microbiology, College of Natural Science,Dankook University) ;
- Kim, Seung-Chul (Department of Microbiology, College of Natural Science,Dankook University) ;
- Ryeom, Kon (Department of Microbiology, College of Natural Science,Dankook University)
- 박준구 (단국대학교 자연과학대학 미생물학과) ;
- 최병돈 (단국대학교 자연과학대학 미생물학과) ;
- 김승철 (단국대학교 자연과학대학 미생물학과) ;
- 염곤 (단국대학교 자연과학대학 미생물학과)
- Published : 1993.10.01
Abstract
An Actinomycete strain isolated from Mt. Dea-Dun had a strong antifungal activity. The culture brith produced by isolated strain showed only antifungal activity against fungi with the exception of yeast and bacteria. It was heat stable, dissolved in ehtylacetate. The concentrated antifungal agent showed cytotoxicity against HEP-2 and HeLa as tumor cell line, and showed weak cytotoxicity against VERO 36 as normal cell line. Morphological and physiological characteristics were tested with isolated strain. The spore color of isolated strain was gray. It had a short chain and produced brown colored lytic substance in yeast extract-malt agar. The cell wall of isolated strain was composed of meso-DAP, and we suggested it as genus Actinomadura. In the existing of chemical inhibitor, isolated strain grew on the condition of 0.0001% crystal violet, 0.1% phenol, 0.01% sodium azide and 10% sodium chloride. Carbon utilization of isolated strain was shown that glucose, sucrose, manitol and sodium citrate were well utilized.
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