• 제목/요약/키워드: Brown line

검색결과 393건 처리시간 0.029초

Inhibition of the expression on MMP-2, 9 and morphological changes via human fibrosarcoma cell line by 6,6'-bieckol from marine alga Ecklonia cava

  • Zhang, Chen;Li, Yong;Shi, Xiujuan;Kim, Se-Kwon
    • BMB Reports
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    • 제43권1호
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    • pp.62-68
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    • 2010
  • Matrix Metalloproteinases (MMPs) are a family of zinc-endopeptidases which can degrade extracellular matrix (ECM) components and play important roles in a variety of biological and pathological processes. 6,6'-bieckol isolated and characterized from an edible marine brown alga Ecklonia cava (EC), according to the comprehensive spectral analysis of MS and NMR data. Here the influence of 6,6'-bieckol on expressions of MMPs was examined by zymography and western blot analysis via human fibrosarcoma cell line (HT1080). It is shown that 6,6'-bieckol significantly down regulated the expressions of MMP-2 and -9 in dose-dependent manner. The influence of 6,6'-bieckol on the cell viability and cell behavior of HT1080 cells were also investigated, our dates shown that it suppressed the migration and 3D culture in HT1080 cells. Meanwhile, we explored several signal pathways which may contribute to this process, and found the suppressing of MMPs expressions in HT1080 cells might be due to the suppression of NF-${\kappa}B$ signal pathway.

토양에서 분리한 방선균의 세포 독성에 관한 연구 (Study of Cytotoxicity of an Actinomycete Isolated in Korea)

  • 박준구;최병돈;김승철;염곤
    • Environmental Analysis Health and Toxicology
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    • 제8권3_4호
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    • pp.7-12
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    • 1993
  • An Actinomycete strain isolated from Mt. Dea-Dun had a strong antifungal activity. The culture brith produced by isolated strain showed only antifungal activity against fungi with the exception of yeast and bacteria. It was heat stable, dissolved in ehtylacetate. The concentrated antifungal agent showed cytotoxicity against HEP-2 and HeLa as tumor cell line, and showed weak cytotoxicity against VERO 36 as normal cell line. Morphological and physiological characteristics were tested with isolated strain. The spore color of isolated strain was gray. It had a short chain and produced brown colored lytic substance in yeast extract-malt agar. The cell wall of isolated strain was composed of meso-DAP, and we suggested it as genus Actinomadura. In the existing of chemical inhibitor, isolated strain grew on the condition of 0.0001% crystal violet, 0.1% phenol, 0.01% sodium azide and 10% sodium chloride. Carbon utilization of isolated strain was shown that glucose, sucrose, manitol and sodium citrate were well utilized.

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상업용 난용종계 집단에서의 유전적 개량량 추정에 관한 연구 (Estimation of Genetic Gains in Commercial Layer Breeding Population)

  • 오봉국;최연호
    • 한국가금학회지
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    • 제17권4호
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    • pp.243-254
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    • 1990
  • 본 연구는 상업용 목적으로 선발, 유지되어 온 계군에서의 선발에 의한 유전모수 추정치의 변화양상을 살펴보고, 추정된 유전모수를 바탕으로 하여 차세대의 유전적 개량량을 예측한 후 이를 선발결과 나타난 관측치와 비교, 분석하기 위하여 수행되었다. 분석에 이용된 자료는 단관백색레그혼종 1계통(Line-W)과 갈색난용종 1계통(Line-B)에 대한 1980년부터 1986년까지의 5세대간 자료로써 표현형 능력변화와 선발형질들에 대한 유전력 및 유전상관계수를 추정하고, 이들을 바탕으로 하여 추적선발지수방법에 의해 유전적 개량량을 추정하였다. 추정된 각 형질의 유전적 재량량을 살펴 보면, 두 계통에서 초산일령의 경우 각각 세대당 1.67일과 1.14일씩 단축된 것으로 나타났고, 300일령 산란수는 각각 4.15개와 2.37개, 400일령 산란수는 각각 5.92개와 3.73개씩 증가된 것으로 분석되었으며, 30주령시 난중과 40주령시 난중은 거의 변화가 없었던 것으로 나타났다. 이상에서 얻어진 각 형질의 유전적 개량량의 수치는 300일령 산란수 한 형질만을 모가계 선발에 의해 선발한 경우의 유전적 개량량과 유사한 것으로 나타났으며, 추정된 유전적 개량량에 의해 차세대의 능력을 예측한 후 이를 관측치와 비교한 결과 선발 후기세 대(4세대와 5세대)에서 두 계군이 환경의 영향을 크게 받았던 것으로 판단되었다.

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제주도 연안에서 채집된 뿔돔과(Priacanthidae) 큰눈홍치 Heteropriacanthus cruentatus (Perciformes: Priacanthidae)의 재기재 (Redescription of the Glasseye Snapper Heteropriacanthus cruentatus (Perciformes: Priacanthidae) from the coastal waters of Jeju Island, Korea)

  • 최윤;이홍헌
    • 한국어류학회지
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    • 제22권2호
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    • pp.126-128
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    • 2010
  • 제주도 근해에서 채집된 1개체(표준체장 179.3 mm)의 표본을 근거로 뿔돔과 큰눈홍치 Heteropriacanthus cruentatus의 형태적 특징을 상세하게 재기재하였다. 본 종은 둥지느러미 연조부와 뒷지느러미, 꼬리지느러미에 흑자색 점들이 산재하고 측선공이 59개인 특징이 있다.

산란종계 육성기 사료의 에너지 및 단백질 수준이 육성기 성장에 미치는 영향

  • 노성래;우상원;유선종;김은집;안병기;강창원
    • 한국가금학회:학술대회논문집
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    • 한국가금학회 2002년도 가을 학술발표논문집
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    • pp.118-119
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    • 2002
  • This experiment was conduct to determine the influences of different levels of dietary energy and crude protein on growth performances during growing period (2 to 10 wk of age). A total of 360 egg breeder chicks (Hy-Line Brown) was divided into 5 groups with 4 replicates of 18 birds per replicate and fed the experimental diets containing three levels of energy and three levels of protein. With the increase in dietary crude protein levels, body weight significantly increased (P〈0.05). But dietary energy levels did not greatly affect growth performances. There were no significant differences to flock uniformity and tibia strength among treatments.

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벤토나이트를 산란노계 사료에 첨가시 계란의 지방산조성에 관한 연구 (A Study on Fatty Acid Profiles of Eggs in Older Laying Hens fed Diets Supplemented with Bentonite)

  • 최인학
    • 한국환경과학회지
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    • 제27권5호
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    • pp.341-344
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    • 2018
  • This study was conducted to determine the effects of dietary bentonite supplementation on the fatty acid profiles of eggs in older laying hens. A total of 90 Hy-line Brown layers, 74 weeks of age, were confined in 6 wire cages and then assigned randomly to two groups to receive one of the two diets (3 replicates of 15 older layers each) containing 0 and 0.2% bentonite for 4 weeks. After the 4-week feeding trial, no remarkable differences in individual fatty acid, saturated fatty acid (SFA), unsaturated fatty acid (UFA), and mono unsaturated fatty acid (MUFA) levels were found. However, linoleic acid, docosahexaenoic acid, and poly unsaturated fatty acid (PUFA) and UFA/SFA ratio were influenced by 0.2% bentonite. In conclusion, supplementation with 0.2% bentonite improved the profiles of PUFA more than those of SFA, UFA, and MUFA inthe eggs of the older laying hens.

Occurrence of Stem Rot of Disporum smilacinum Caused by Sclerotium rolfsii in Korea

  • Kwon, Jin-Hyeuk;Jee, Hyeong-Jin
    • The Plant Pathology Journal
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    • 제23권3호
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    • pp.212-214
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    • 2007
  • In 2005 and 2007, a basal stem rot of Disporum smilacinum caused by Sclerotium rolfsii occurred sporadically in a herb farm at Hamyang, Korea. The symptom initiated with water-soaking lesion and progressed into stem rot and wilt of a whole plant. Severely infected plants were blighted and died eventually. White mycelial mats appeared on the lesion at early stage and a number of sclerotia were formed on the stem near the soil line. The sclerotia were globoid in shape, 1-3 mm in size and white to brown in color. The optimum temperature for the growth and sclerotia formation was 30 on PDA and the hyphal width was measured $3-8{\mu}m$. The typical clamp connections were observed in the hyphae of the fungus grown on PDA. On the basis of symptom, mycological characteristics and pathogenicity to the host plant, this fungus was identified as Sclerotium rolfsii Saccardo. This is the first report on the stem rot of D. smilacinum caused by S. rolfsii in Korea.

Three Juvenile Snappers of the Genus Lutjanus (Perciformes: Lutjanidae) Collected from Jeju Island, Korea

  • Kim, Byung-Jik;Nakaya, Kazuhiro;Endo, Hiromitsu
    • Fisheries and Aquatic Sciences
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    • 제10권2호
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    • pp.68-73
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    • 2007
  • Based on four juvenile specimens of Lutjanus collected from the southern coast of Jeju Island, three species, L. argentimaculatus, L. fulviflamma, and L. quinquelineatus, are described as the first record from Korea. The first species is easily differentiated from other lutjanid juveniles by distinct coloring of the dorsal, pelvic, and anal fins as well as two blue lines below the eye; the second species is characterized by having six yellowish brown stripes below the lateral line and a black spot on the side; the third species is identified by five longitudinal blue stripes and a black spot on the side when fresh. The new Korean names "Bul-geun-tung-dom," "Yuk-seon-jeomtung-dom," and "O-seon-tung-dom" are proposed for L. argentimaculatus, L. fulviflamma, and L. quinquelineatus, respectively.

수산화가스 에너지를 이용한 용접시스템 개발 (Development of welding machine using Hydrogen-Oxygen-Gas)

  • 이용균;정병환;전윤석;목형수;최규하
    • 전력전자학회:학술대회논문집
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    • 전력전자학회 2003년도 추계학술대회 논문집
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    • pp.77-80
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    • 2003
  • As rapid growth of energy demand in line with modern society's industrialization led to environmental pollution by fossil fuels, there are more and more interest and international research on energization of water, which is clean energy and comprising $70\%$ of earth. In offshore countries, the water is commercially used already and water has very attractive characteristics in terms of economy and efficiency compared to the existing gas welder. Brown Gas welder does not produce $CO_2$ from combustion of Hydrogen-Oxygen-Gas, which was gained by electrolysis of water, In this paper, the result from operating characteristic improvement of Gas Generator and Current-Controlled converter is to be considered into design in electrode, source/ controller, standized into level of whole system design base.

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고추씨의 영양적 가치 평가와 사료 내 첨가가 산란계의 생산성에 미치는 영향

  • 장익훈;노성래;권순관;김은집;임호중;강창원
    • 한국가금학회:학술대회논문집
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    • 한국가금학회 2001년도 제18차 정기총회 및 학술발표 PROCEEDINGS
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    • pp.54-55
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    • 2001
  • The experiments were conducted to evaluate the feeding values of red pepper seed (Exp. 1) and to investigate its dietary supplementation on layer performances (Exp. 2). The values of TME, TMEn and average TAAA determined by force-feeding 14 roosters (Hy-line) were 2.83 kcal/g, 2.94 kcal/g and 82.72%, respectively. a total of 200 layers (ISA-brown) was divided into 4 groups with 5 replicates of 10 birds per replicate, and fed the experimental diets containing different level of red pepper seed (0, 3, 5, 10%). There were no differences in performances of laying hens among treatments. Liver lipids in all groups containing red pepper seed were significantly reduced as compared to those of control (P<0.05).

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