• Journal of Mushroom
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• v.13 no.3
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• pp.237-242
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• 2015

A new brown button mushroom cultivar, 'Hogam', C34 line, was made by crossing homokaryons, ASI1164-37 and ASI1175-66, selected by RAPD analysis and by cultivating three times. Mycelium of 'Hogam' on CDA (compost dextrose agar) media grew well at $25^{\circ}C$. The optimum pin-heading temperature of new variety and optimum growing temperature was $14-18^{\circ}C$. The thickness of the mature cap and stipe were thicker than a control, 'Dahyang' that developed in 2010. The color of pileus was light brown and lighter than 'Dahyang'. Days required from casing to first harvesting were three days longer than control strain, but the weight of harvested fruiting body increased by 1.35 times. 'Hogam' cultivar are expected to contribute to the diversification of domestic mushroom cultivars.

• Journal of Mushroom
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• v.19 no.3
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• pp.246-250
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• 2021

The main characteristics of a new cultivar 'Jinhyang', a cross of a collection of monokaryotic strains of the brown button mushroom, were examined. 'Jinhyang' was not significantly different from the control cultivar 'Dahyang' in mycelial culture and cultivation period. The cap of 'Jinhyang' was thicker and darker in color than that of 'Dahyang'. The hardness and individual weight of the fruiting body of 'Jinhyang' were slightly higher than those of 'Dahyang'; however, the differences were not significant. The yield of 'Jinhyang' was 14.1 kg/m², which was 8% higher than that of 'Dahyang'. Therefore, it is expected that it will be possible to supply this cultivar growers in the future.

• Journal of Mushroom
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• v.9 no.1
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• pp.17-21
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• 2011

Dahyang, a CNARES's newly bred cultivar, is a CM061202 strain isolated and selected from 161 single-spores of brown button mushroom. It was selected as a new cultivar after comparison of yield, mycelial growth and quality with No. 705. Mycelial growth of Dahyang on compost extract mushroom complete medium at $25^{\circ}C$ for 15 days was 63mm when it was compared with that of No. 705 with 38mm. The cultivation temperature of Dahyang was 15 to $19^{\circ}C$ which was $2^{\circ}C$ higher than that of No. 705 with 15 to $19^{\circ}C$ cultivated on rice straw bed. The color of pileus of Dahyang was brown. Pinhead producing period was 30 days which is 1 day late than that of No. 705 with 29 days. Dahyang yielded 37% more with $49.1kg/3.3m^2$ than that of No. 705 with $35.8kg/3.3m^2$, respectively. The weight was 16.0g which was heavier than 14.0g of No. 705. The thickness and diameter were also more than those of No. 705. The stipe was shorter and thicker than that of No. 705. Also physical characteristics such as hardness and elasticity were better than that of No. 705.

• Journal of Mushroom
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• v.16 no.3
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• pp.175-179
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• 2018

The button mushroom 'A. bisporus' is the most commonly grown mushroom worldwide. The color of 'Agaricus bisporus' is white and brown. Recently, the consumption of brown button mushrooms has been increasing steadily. 'Danseok-1ho' was bred using the multi-spore random mating method and named after Danseok Mountain in Gyeongju, the region where 'A. bisporus' was first grown in Korea. The optimum temperature for mycelial growth was between $20^{\circ}C$ and $30^{\circ}C$, and the mushroom production temperature was between $15^{\circ}C$ and $20^{\circ}C$. The color and shape of 'Danseok-1ho' are medium brown and spherical, respectively. The width and thickness of the pileus are 45.1 mm and 23.5 mm, respectively. The hardness and L of chromaticity of 'Danseok-1ho' are $8,937g/{\Phi}5mm$ and 50.4, respectively. The incubation period is around 20 days and mushroom production takes about 18 days after casing. A typical characteristic of 'Danseok-1ho' is that the individual occurrence rate is high, so mushroom losses are low during harvest.

• Journal of Mushroom
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• v.14 no.3
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• pp.81-89
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• 2016

White button mushroom (Agaricus bisporus) is the most widely consumed mushroom in the world, and domestic consumption is increasing owing to expansion of the westernized diet in recent years. Before 2000, most Korean mushroom farmers cultivated foreign varieties, but recently, Korean breeders are developing hybrid strains using molecular breeding tools. To produce a better mushroom cultivar, we evaluated some traits of button mushroom Agaricus bisporus strains. Mycelial growth rate at different culture temperatures was investigated by 25>20>15>30>$10^{\circ}C$. Recently developed domestic varieties had superior mycelial growth rate compared to the traditionally grown species. In particular, the ASI1338 strain showed excellent mycelial growth rate at different temperatures. Further, we confirmed that ASI1007, ASI1085, ASI1310, ASI1339 were strongly resistant to bacterial brown blotch, whereas ASI1053, ASI1103, ASI1140, ASI1146, ASI1177, ASI1183, ASI1195, ASI1321, ASI1331, ASI1336 were relatively weakly resistant. The average number of days for 16.5, and that for harvest was 5.7; the average yield was 142.7 g/2-kg bag. Mycelial growth rates at 10, 15, 20, $25^{\circ}C$ were similar, but that at $30^{\circ}C$ was lower. The mushroom yield was highly correlated with the number of days for pinheading and harvest.

• Mycobiology
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• v.49 no.2
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• pp.173-182
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• 2021

Recently, Cryptococcus pseudolongus has been reported as a new pathogen of shiitake (Lentinula edodes). However, its pathological properties are not much known. To further understand its impact on the mushroom, we investigated the pathogen's interactions with the mycelium of shiitake, histopathological properties, host range, and sensitivity to diverse antifungal agents. The strain C. pseudolongus DUCC 4014 inhibited the mycelial growth of L. edodes strain (cultivar Sanjo 701ho) and caused browning in the mycelia confronted with the yeast on PDA. Spray inoculation of the yeast caused an abnormal browning symptom on the cap and/or gills of three shiitake cultivars grown on sawdust media in vinyl bags. Scanning electron microscopic images of the abnormally browned parts of shiitake fruit body illustrated that mushroom tissues were loosed and dispersed in the middle and edge of the cap and the arrangement of basidiospores borne on basidia in the gills was disturbed compared to those of normal shiitake fruit body. Spray inoculation also led to developing abnormal browning on the harvested fruit body, indicating C. pseudolongus could be a problem during mushroom storage. But the yeast was not able to induce abnormal browning on mushrooms of Pleurotus ferulae, Pleurotus fostreatus, and Agaricus bisporus. But it induced browning only on button mushroom (A. bisporus) when they were inoculated after wounding. Tests with 16 kinds of fungicides revealed that the cell growth of C. pseudolongus could be inhibited by benzalkonium chloride at MIC 7 ㎍/ml and benomyl at MIC 3 ㎍/ml.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.8
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• pp.1179-1183
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• 2011

This study was conducted to investigate the quality characteristics, antioxidant activity, and ${\alpha}$-glucoamylase inhibitory activity of Dahyang, a Chungnam Agricultural Research & Extension Service's newly bred cultivar of brown button mushroom. Total phenolic compound contents of Dahyang and the no. 705 mushroom were 189${\pm}$12 mg% and 168${\pm}$8 mg%, respectively. The major free sugars in Dahyang were mannitol (3.11%), xylose (0.12%), and trehalose (0.08%). ${\beta}$-Glucan content was 28.34% in Dahyang and 26.55% in the no. 705 mushroom, respectively. Electron donating ability by DPPH in Dahyang and the no. 705 mushroom was 52.14% and 45.27% for the water extract, and 57.81% and 46.93% for the 80% ethanol extract, respectively. ${\alpha}$-Glucoamylase inhibitory activity in a 10 mg/mL concentration of water extract were was 33.25% in Dahyang and 29.22% in the no. 705 mushroom, respectively.