• Applied Microscopy
• /
• v.51
• /
• pp.4.1-4.12
• /
• 2021

Fluorescence in situ hybridization (FISH) is a technique to visualize specific DNA/RNA sequences within the cell nuclei and provide the presence, location and structural integrity of genes on chromosomes. A confocal Whole Slide Imaging (WSI) scanner technology has superior depth resolution compared to wide-field fluorescence imaging. Confocal WSI has the ability to perform serial optical sections with specimen imaging, which is critical for 3D tissue reconstruction for volumetric spatial analysis. The standard clinical manual scoring for FISH is labor-intensive, time-consuming and subjective. Application of multi-gene FISH analysis alongside 3D imaging, significantly increase the level of complexity required for an accurate 3D analysis. Therefore, the purpose of this study is to establish automated 3D FISH scoring for z-stack images from confocal WSI scanner. The algorithm and the application we developed, SHIMARIS PAFQ, successfully employs 3D calculations for clear individual cell nuclei segmentation, gene signals detection and distribution of break-apart probes signal patterns, including standard break-apart, and variant patterns due to truncation, and deletion, etc. The analysis was accurate and precise when compared with ground truth clinical manual counting and scoring reported in ten lymphoma and solid tumors cases. The algorithm and the application we developed, SHIMARIS PAFQ, is objective and more efficient than the conventional procedure. It enables the automated counting of more nuclei, precisely detecting additional abnormal signal variations in nuclei patterns and analyzes gigabyte multi-layer stacking imaging data of tissue samples from patients. Currently, we are developing a deep learning algorithm for automated tumor area detection to be integrated with SHIMARIS PAFQ.

• Transactions of the Korean Society of Automotive Engineers
• /
• v.14 no.4
• /
• pp.39-48
• /
• 2006

In this research, in order to study the spray, combustion, and emission characteristics of the common rail DME engine, the target engine was disassembled, and 3D CAD file was constructed using a 3D measurement machine and a rapid prototyping machine. Using the obtained 3D geometry, fine moving meshes are generated, and three dimensional non-steady turbulence flow field and combustion phenomenon including spray were numerically analyzed. As a result, IMEP of DME and diesel in medium and high speed revolution showed similar performance. As the DME fuel start to burn in spray area, the vaporized fuel rapidly spreads squish area in low speed revolution. In the case of DME engine, CO and NOx are relatively consistent with experiment results. It was found that the break-up, evaporation, collision model of DME fuel need to be properly adjusted through matching the characteristics of fuel and injector for further improvement.

• Journal of Biosystems Engineering
• /
• v.31 no.2 s.115
• /
• pp.95-101
• /
• 2006

Present chemical application system using a power sprayer has been a labor intensive, ineffective and shirking task in farming. Therefore, a small RF controlled (unmanned) helicopter was suggested to replace the conventional spray system. The aerial application using the unmanned helicopter has been already utilized in Japan, where total area applied up to 704,000 ha in 2005. In this article, the status and cost analysis on the unmanned agricultural helicopter were studied. The aerial application using the agricultural helicopter helps precise and timely spraying and reduces labor intensity and pollution. The field capacity of the helicopter was found to be 60-70 ha a day. The break even point was estimated near the operating area of 750 ha annum. The development of an agricultural helicopter was necessary for taking advantages of both technique and economy.

• Journal of Advanced Navigation Technology
• /
• v.17 no.1
• /
• pp.132-141
• /
• 2013

In this paper, method using electric braking from stop area to high-speed area was presented in order to improve air-brake. And electric braking method can be improved environmental problems, efficiency, economy, etc. Method for electrical complete braking are two ways that method of inserting series resistance between the motor and the inverter, and method of inverter output voltage increase. In this paper, use series resistance insert method because economical and easy to apply. In addition, Series resistor is used short circuit method for reduce the power loss. In improved efficiency and the laboratory environment for secure braking, resistance insert method and inverter output voltage increase method showed same characteristics in all areas.

• Journal of the Korean Society of Safety
• /
• v.24 no.5
• /
• pp.63-68
• /
• 2009

The urgent VAI method development is required since "The Act of Physical Protection and Radiological Emergency that is established in 2003" requires an evaluation of physical threats in nuclear facilities and an establishment of physical protection in Korea. The VAI methodology is developed to (1) make a sabotage model by reusing existing fire/flooding/pipe break PSA models, (2) calculate MCSs and TEPSs, (3) select the most cost-effective TEPS among many TEPSs, (4) determine the compartments in a selected TEPS as vital areas, and (5) provide protection measures to the vital areas. The developed VAI methodology contains four steps, (1) collecting the internal level 1 PSA model and information, (2) developing the fire/flood/pipe rupture model based on level 1 PSA model, (3) integrating the fire/flood/pipe rupture model into the sabotage model by JSTAR, and (4) calculating MCSs and TEPS. The VAT process is performed through the VIPEX that was developed in KAERI. This methodology serves as a guide to develop a sabotage model by using existing internal and external PSA models. When this methodology is used to identify the vital areas, it provides the most cost-effective method to save the VAI and physical protection costs.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• v.28 no.4
• /
• pp.256-263
• /
• 2002

Using the rat's skull, the study on the biodegradability and guided bone regeneration of the chitosan membrane was performed. The results are as follows: 1. The biodegradability of the chitosan membrane could not be confirmed, but after 12 weeks, this membrane did not yet break into small pieces and there was no specific local tissue reaction. 2. It was not certain whether the pore size of this membrane was affected on osteoblastic activity. 3. After 6 weeks, the bony defect area of rat's skull was not completely filled, but on high magnification it showed that the osteoclasts and the osteoblasts were observed in the regenerating area. In conclusion, the chitosan membrane developed in this study was fit for guided bone regeneration.

• Asian-Australasian Journal of Animal Sciences
• /
• v.14 no.8
• /
• pp.1098-1105
• /
• 2001

There is little information on smallholder dairy farming in Zimbabwe. With such inadequate knowledge, no meaningful decisions on how to improve these systems can be made. A study was, therefore, carried out in Nharira communal area and Lancashire small-scale commercial area to provide information on the viability of dairy farms. This paper is based on data obtained through participatory monitoring of 13 smallholder dairy farms in 1996 and 1997. All the four farms in Lancashire were found to be viable in both years. In Nharira, two out of the nine farms in 1996 and three farms in 1997 failed to break even. There were considerable inter-farm differences in the contribution of milk sales towards total income from dairy farming, ranging from 41% to 99% in Nharira and 71% to 81% in Lancashire in 1996. Corresponding estimates in 1997 were 51-95% and 72-78%, respectively. Expenses on cattle feeds contributed 36-84% in 1996 and 37-80% in 1997 towards total variable costs in Nharira. In Lancashire, the respective estimates were 15-33% and 22-36%. Seven out of the nine studied farms in Nharira and three out of four in Lancashire realized higher gross margin (GM) in 1997 than in 1996. All these farming households had conserved considerable amounts of farm-grown feeds. It was concluded that feed costs, number of cows and sizes of land holdings were the main factors determining viability of smallholder dairy farming. The major challenge to smallholder dairy farming in Nharira, in particular, was to develop low-cost feeding strategies.

• Journal of the Korean Institute of Rural Architecture
• /
• v.16 no.1
• /
• pp.87-94
• /
• 2014

The objective of this study is to provide a townscape design guideline that harmonized with a historic landscape via condition investigation and landscape analysis of the cultural /commercial environment district where an improvement is a necessity. The followings are the conclusions: 1.To enhance the identity of Gyeongju, should pursue diversity in unity by making the image of architectural landscape a similar peculiarity. 2.The central commercial district where the tradition is valued and contemporary figures meet the tradition needs a landscape formation by CONTEXT. 3.Since a characteristic of the target area is a regional commercial center, to reduce its congestion, reforming the area to simple environment is required. 4.Induce buildings located on the streets with cultural properties to have Korean traditional tiled roof in order to fit in with their surroundings. 5.Make it a rule to partition a wall into tripartition, the wall surface exposed should be the same finishing materials. 6.Consider the visual aspect of pedestrians and a building's width, the recommendable height of the facade should be 3~3.6m. 7.For the design archetype of cornice, four traditional types based on Korean traditional eaves are suggested. 8.The design of signboards should break existing square-shapes, and seek a design to take advantage of icons that historicity and traditionalist of the city are expressed.

• Nuclear Engineering and Technology
• /
• v.48 no.5
• /
• pp.1264-1272
• /
• 2016

The accurate estimation of leak rate through cracks is crucial in applying the leak before break (LBB) concept to pipeline design in nuclear power plants. Because of its importance, several programs were developed based on the several proposed flow models, and used in nuclear power industries. As the flow models were developed for a homogeneous pipe material, however, some difficulties were encountered in estimating leak rates for bimaterial pipes. In this paper, a flow model is proposed to estimate leak rate in bimaterial pipes based on the modified Henry-Fauske flow model. In the new flow model, different crack morphology parameters can be considered in two parts of a flow path. In addition, based on the proposed flow model, a program was developed to estimate leak rate for a crack with linearly varying cross-sectional area. Using the program, leak rates were calculated for through-thickness cracks with constant or linearly varying cross-sectional areas in a bimaterial pipe. The leak rate results were then compared and discussed in comparison with the results for a homogeneous pipe. The effects of the crack morphology parameters and the variation in cross-sectional area on the leak rate were examined and discussed.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
• /
• v.5 no.4
• /
• pp.191-197
• /
• 2012

In this paper, method using electric braking from stop area to high-speed area was presented in order to improve air-brake. And electric braking method can be improved environmental problems, efficiency, economy, etc.. Method for electrical complete braking are two ways that method of inserting series resistance between the motor and the inverter, and method of inverter output voltage increase. In this paper, use series resistance insert method because economical and easy to apply. In addition, Series resistor is used short circuit method for reduce the power loss. In improved efficiency and the laboratory environment for secure braking, resistance insert method and inverter output voltage increase method showed same characteristics in all areas.