• Title/Summary/Keyword: Branching Method

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Phenotypic and molecular characteristics of second clone (T0V2) plants of the LeLs-antisense gene-transgenic chrysanthemum line exhibiting non-branching (무측지성 국화 형질전환 계통 영양번식 제2세대의 형태적 및 분자생물학적 특성)

  • Lee, Su Young;Kim, Jeong-Ho;Cheon, Kyeong-Seong;Lee, Eun Kyung;Kim, Won Hee;Kwon, O Hyeon;Lee, Hye Jin
    • Journal of Plant Biotechnology
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    • v.40 no.4
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    • pp.192-197
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    • 2013
  • This study examined the phenotypic and molecular characteristics of the $2^{nd}$ clone ($T_0V_2$) plants of LeLs-antisense gene-transgenic chrysanthemum line (LeLs80) that exhibited non-branching, proving the relevance of these characteristics as a factor for use in environmental risk assessment. Results of the Southern blot analysis showed that three copies of the LeLs-antisense gene were introduced into the transgenic line, and northern analysis showed that the transcripted gene was normally expressed in the transgenic line. A flanking T-DNA sequencing method was used to determine that sequences of 184 and 464 bps flanked the LeLs-antisense gene in the transgenic line. These sequences, respectively, matched the 35S promoter for expression of the npt II gene and the NOS terminator for expression of the LeLs-antisense gene within the pCAMBIA 2300 vector.

Three-Dimensional Flow Visualization for the Steady and Pulsatile Flows in a Branching Model using the High-Resolution PIV System

  • Suh, Sang-Ho;Roh, Hyung-Woon
    • International Journal of Vascular Biomedical Engineering
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    • v.2 no.2
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    • pp.27-32
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    • 2004
  • The objective of the present study is to visualize the steady and pulsatile flow fields in a branching model by using a high-resolution PIV system. A bifurcated flow system was built for the experiments in the steady and pulsatile flows. Harvard pulsatile pump was used to generate the pulsatile velocity waveforms. Conifer powder as the tracing particles was added to water to visualize the flow fields. CCD cameras($1K{\times}1K$(high resolution camera) and $640{\times}480$(low resolution camera)) captured two consecutive particle images at once for the image processing of several cross sections on the flow system. The range validation method and the area interpolation method were used to obtain the final velocity vectors with high accuracy. The results of the image processing clearly showed the recirculation zones and the formation of the paired secondary flows from the distal to the apex of the branch flow in the bifurcated model. The results also indicated that the particle velocities at the inner wall moved faster than the velocities at the outer wall due to the inertial force effects and the helical motions generated in the branch flows as the flow proceeded toward the outer wall. Even though the PIV images from the high resolution camera were closer to the simulation results than the images from the low resolution camera at some locations, both results of the PIV experiments from the two cameras generally agreed quite well with the results from the computer simulations. Therefore, instead of using the expensive stereoscopic PIV or 3D PIV system, the three-dimensional flow fields in a bifurcated model could be easily and exactly investigated by this study.

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Morphological Changes in the Mammary Organ Culture of the Rat Treated with 7,12-Dimethylbenz[$\alpha$]anthracene and N-methyl-N-nitrosourea (7,12-Dimethylbenz[$\alpha$anthracene 및 N-methyl-N-nitrosourea를 투여한 랫드 유선 조직 배양에 대한 형태학적 변화)

  • 문지영;정자영;김옥희;이형환
    • Toxicological Research
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    • v.16 no.4
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    • pp.275-284
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    • 2000
  • The organ culture model of the whole mammary gland has many advantages for the study of branching morphogenesis and biological characteristics, including tumorigenesis. Prior to whole gland organ culture, rats were treated with 7,12-dimethylbenz[a]anthracene (DMBA) or N-methyl-N-nitrosourea (MNU) for one week. The tramdorming effect and the morphological changes were assessed by the whole mount preparations and histopathological examination in terminal end buds (TEB), terminal ducts (TD), alveolar buds (AB), alveolar lobules (AL) and hyperplastic alveolar nodules (HAN) of the mammary gland. Grossfindings of the mammary glands at dissection were higher branching morphogenesis and larger volume in carcinogen-treated groups than in carcinogen-non-treated groups. Results of the whole mount method were coincided with those of the histopathological observations. Circular TEB, normally maintained AB, AL, and high cellular density were more frequently observed in carcinogen-treated groups than in carcinogen-nan-treated groups. Histopathologically, as a preneoplastic marker, HAN was maintained only in mammary organ culture of the carcinogen-treated groups. These findings suggest that in vivo trans-formation effects by carcinogens persisted during the mammary organ culture. These results were more characteristic in DMBA than in MNU-treated group. Ducts and terminal ducts appeared to have lost morphology during their growths in case of without diethylstilbestrol (DES). The fact that in vitro organ culture without DES was resulted in abnormal ductular morphogenesis confirms that DES is a physiological regulator of ductular epithelial cell growth.

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Peridynamic Modeling for Crack Propagation Analysis of Materials (페리다이나믹 이론 모델을 이용한 재료의 균열 진전 해석)

  • Chung, Won-Jun;Oterkus, Erkan;Lee, Jae-Myung
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.31 no.2
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    • pp.105-114
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    • 2018
  • In this paper, the computer simulations are carried out by using the peridynamic theory model with various conditions including quasi-static loads, dynamic loads and crack propagation, branching crack pattern and isotropic materials, orthotropic materials. Three examples, a plate with a hole under quasi-static loading, a plate with a pre-existing crack under dynamic loading and a lamina with a pre-existing crack under quasi-static loading are analyzed by computational simulations. In order to simulate the quasi-static load, an adaptive dynamic relaxation technique is used. In the orthotropic material analysis, a homogenization method is used considering the strain energy density ratio between the classical continuum mechanics and the peridynamic. As a result, crack propagation and branching cracks are observed successfully and the direction and initiation of the crack are also captured within the peridynamic modeling. In case of applying peridynamic used homogenization method to a relatively complicated orthotropic material, it is also verified by comparing with experimental results.

An efficient search of binary tree for huffman decoding based on numeric interpretation of codewords

  • Kim, Byeong-Il;Chang, Tae-Gyu;Jeong, Jong-Hoon
    • Proceedings of the IEEK Conference
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    • 2002.07a
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    • pp.133-136
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    • 2002
  • This paper presents a new method of Huffman decoding which gives a significant improvement of processing efficiency based on the reconstruction of an efficient one-dimensional array data structure incorporating the numeric interpretation of the accrued codewords in the binary tree. In the Proposed search method, the branching address is directly obtained by the arithematic operation with the incoming digit value eliminating the compare instruction needed in the binary tree search. The proposed search method gives 30% of improved Processing efficiency and the memory space of the reconstructed Huffman table is reduced to one third compared to the ordinary ‘compare and jump’ based binary tree. The experimental result with the six MPEG-2 AAC test files also shows about 198% of performance improvement compared to those of the widely used conventional sequential search method.

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Frontal Flow Field Construction for Wall Boundary Condition Treatment and Frontal Remeshing Using Spline Curve in Injection Molding Simulation (사술성형 모사에 있어서 벽면 경계조건 처리를 위한 선단 유동장 생성기법과 spline 곡선을 이용한 선단 격자 재구성)

  • 윤재륜
    • The Korean Journal of Rheology
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    • v.5 no.1
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    • pp.34-48
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    • 1993
  • 최근 CAD/CAM의 발전과 더불어 사출성형공정은 여러분야에 폭넓게 응용되고 있 다. 사출성형공정은 크게 충전과정(filling stage), 냉각과정(cooling stage), 보압과정(packing stage)로 나누어 지는데 이중 충전과정은냉각과정과 보압과정에서 나타날 물리적인 현상과 최종 성형품의 기계적 성질에 중요한 영향을 끼치게 된다. 충전과정의 수치 해석 방법은 대 표적으로 control volume method, branching flow method, transient moving boun-dary method로 구분된다. 본 연구에서는 격자의 형태를 양호하게 형성시키고 유동선단의 형태를 개선하기위한 기법인 Spline 곡선을 이용한 선단격자 재구성(frontal remeshing using spline curve)과 수치해석에 소요되는 시간을 줄이기 위하여 벽면경계조건 처리를 위한 선단 유동 장생성(frontal flow field construction for wall boun-dary condition treatment)기법을 개발 하고 transient moving voundary method에 적용시켜 원형 평판과 인장 및 굽힘시편 그리고 두께가 변하는 사각 형상을 가진 캐비터에서의 충전과정을 수치해석하였다. 그결과 압력 분 포, 온도분포, 속도장, 유동선단의 진전형태 등이 기존에 제출된 해석결과와 비교하여 볼 때 만족스러운 수치해석결과를 보였다.

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System Reliability Analysis of Midship Sections (선체 중앙 횡단면의 시스템 신뢰성해석)

  • Y.S. Yang;Y.S. Suh
    • Journal of the Society of Naval Architects of Korea
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    • v.30 no.1
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    • pp.115-124
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    • 1993
  • A structural system reliability analysis is studied for the safety assessment of midship section. Probabilistically dominant collapse modes are generated by Element Replacement Method and Incrimental Load Method. In order to avoid generating the same modes repeatedly, it is branched at final plastic hinge. Using first and second order bound methods, system failure probability of midship section is computed and compared with deterministic load factor method to show the usefulness of the proposed method.

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Discrete crack analysis for concrete structures using the hybrid-type penalty method

  • Fujiwara, Yoshihiro;Takeuchi, Norio;Shiomi, Tadahiko;Kambayashi, Atsushi
    • Computers and Concrete
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    • v.16 no.4
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    • pp.587-604
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    • 2015
  • The hybrid-type penalty method (HPM) is suitable for representing failure phenomena occurring during the transition from continua to discontinua in materials such as concrete. Initiation and propagation of dominant cracks and branching of cracks can easily be modeled as a discrete crack. The HPM represents a discrete crack by eliminating the penalty that represents the separation of the elements at the intersection boundary. This treatment is easy because no change in the degrees of freedom for the discrete crack is necessary. In addition, it is important to evaluate the correct deformation of the continua before the crack formation is initiated. To achieve this, we implemented a constitutive model of concrete for the HPM. In this paper, we explain the implemented constitutive model and describe the simulation of an anchor bolt pullout test using the HPM demonstrating its capability for evaluating progressive failure.

Analysis of GIUH Model using River Branching Characteristic Factors (하천분기 특성인자를 고려한 지형학적 순간단위도 모형의 해석)

  • Ahn, Seung-Seop;Kim, Dae-Hyeung;Heo, Chang-Hwan;Park, Jong-Kwon
    • Journal of the Korean Association of Geographic Information Studies
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    • v.5 no.4
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    • pp.9-23
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    • 2002
  • The purpose of this research was to develop a model that minimizes time and money for deriving topographical property factors and hydro-meteorological property factors, which are used in interpreting flood flow, and that makes it possible to forecast rainfall-runoff using a least number of factors. That is, the research aimed at suggesting a runoff interpretation method that considers the river branching characteristics but not the topographical and geological properties and the land cover conditions, which had been referred in general. The subject basin of the research was the basin of Yeongcheon Dam located in the upper reaches of the Kumho River. The parameters of the model were derived from the results of abstracting topological properties out of rainfall-runoff observation data about heavy rains and Digital Elevation Modeling(DEM). According to the result of examining calculated peak runoff, the Clark Model and the GIUH Model showed relative errors of 1.9~23.9% and 0.8~11.3%, respectively and as a whole, the peak values of hydrograph appeared high. In addition, according to the result of examining the time when peak runoff took place, the relative errors of the Clark Model and the GIUH Model were 0.5~1 and 0~1 hour respectively, and as a whole, peak flood time calculated by the GIUH Model appeared later than that calculated by the traditional Clark Model.

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An Experimental Study on the Improvement of Foam Fire-Extinguishing System's Mixing Ratios by Expanding the Cross Sectional Area of the Stock Solution Inhaling Piping (원액흡입배관 단면적 확장에 의한 포 소화설비 혼합비 개선에 관한 실험적 연구)

  • Yun, Ki-Jo;Jang, Kyung-Nam;Baek, Eun-Sun;Park, Bong-Rae;Park, Hee-Joog
    • Fire Science and Engineering
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    • v.27 no.3
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    • pp.30-37
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    • 2013
  • The ultimate purpose of the present study is to secure a effective method for foam liquid discharge when the mixing ratio deviates from the permissible range due to a decrease in the bypass flow rate resulting from a decrease in the cross sectional area of the foam liquid piping ranging from the branching header of one foam proportioner to the branching headers of multiple branching foam fire-extinguishing systems in the region for fire extinguishing and then to the standpipe at the lower part of the storage tank when a fire occurred in a combustible tank. To this end, the cause of mixing ratio variations following changes in the flow rates of existing foam fire extinguishing systems was analyzed, methods for compensation for constant mixing ratios were investigated, and it was proved that metering orifice replacements that could expand the cross sectional area of the stock solution inhaling piping was the most effective way for the improvement of form fire extinguishing systems' mixing ratios through foam proportioner venturi, foam chamber orifice, and metering orifice replacement experiments.