• 대한한의학회지
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• 제20권2호
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• pp.141-145
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• 1999

The aim of this experiment was to investigate the effects of a mixture of Scutellariae Radix, Coptidis Rhizoma and Coptidis Rhizoma water extracts on the blood pressure, plasma renin activity, plasma levels of aldosterone and atrial natriuretic peptide (ANP) in spontaneously hypertensive rats (SHR). The results of this study were as follows: 1. Systolic blood pressure decreased significantly after the administration of a mixture of Scutellariae Radix, Coptidis Rhizoma, and Coptidis Rhizoma water extract 0.2 ml/200g. 2. Plasma renin activity and plasma levels of ANP were not changed after the administration of a mixture of Scutellariae Radix, Coptidis Rhizoma and Coptidis Rhizoma water extracts, 3. Plasma levels of aldosterone was increased significantly after the administration of a mixture of Scutellariae Radix, Coptidis Rhizoma and Coptidis Rhizoma water extracts 0.1 ml/200g.

• 한국어병학회지
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• 제15권2호
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• pp.57-64
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• 2002

Phenanthrene-induced changes in blood organic components. transaminase and ALP activities of Paralichthys oIivaceus were investigaled. The aim of this study was to obtain a holistic view of the toxic responses, and compensations of fish exposed to waterborne phenanthrene. Fish were exposed to 0, 0.5, 1, 2$\mu$M of phenanthrene for 4 weeks. The most noticeable changes were concentration-dependent increase in levels of blood GOT and GPT activities. At concentrations 1.0 and 2.0 $\mu$M of phenanthrene GOT, GPT and ALP enzyme activities in plasma were increased. Plasma albumin concentration was stable in phenanthrene-exposed fish, however, plasma total protein level was reduced significantly at the 4-week sampling points (2.0$\mu$M). Plasma glucose levels were found to be increased significantly over the cootrol throughout duration of the experiment with phenanthrene. Overall the results suggest that phenanthrene has the potential to alter some physiological functions in olive flounder.

• Journal of Community Nutrition
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• 제8권4호
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• pp.207-213
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• 2006

The present study was conducted to investigate the effect of oolong tea extract on blood glucose level and antioxidant system in diabetic rats. The Sprague-Dawley rats were fed on AIN-76 based experimental diets containing 1 % oolong tea extract for 6 weeks. They were induced to be diabetic by receiving streptozotocin (45mg/kg BW) intramuscularly. Blood glucose, blood and hepatic concentration of vitamins A and E, and antioxidant enzyme activities were measured. Oolong tea extract feeding decreased the plasma glucose in diabetic rats. Dietary supplementation of oolong tea extract did not affect antioxidative enzyme activities such as superoxide dismutase, glutathione peroxidase and catalase in diabetic rats. The plasma level of retinol was increased in diabetic rats by feeding oolong tea extract. Plasma and hepatic levels of ${\alpha}$-tocopherol were higher in diabetic rats fed oolong tea extract. In conclusion, these results suggest that oolong tea extract consumption might reduce the plasma glucose in diabetic rats and protect the oxidative damage from diabetic stress to some extent.

• Journal of Acupuncture Research
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• 제23권3호
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• pp.165-175
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• 2006

Objectives: The purpose of this study was to compare Taebaek(SP3) Sinmun(HT7), Daedon(LR 1) Yongcheon(KI1) on the Blood Pressure in Hypertensive RAT induced by 2K1C. Methods : This experiments was to investigate the effects of Herbal-Acupuncture at Taebaek(SP3) Sinmun(HT7), Daedon(LR1) Yongcheon(KIl) on the Blood Pressure, Cardiomegalic index, plasma levels of renin, plasma levels of atrial natriuretic peptide(ANP), serum levels of potassium, and serum levels of aldosterone in Hypertensive RAT induced by 2KlC. Results : The results were as follows. I. Blood Pressure was decreased significantly after Herbal-Acupuncture of Taebaek(SP3) Sinmun(HT7), Daedon(LR1) Yongcheon(KI1). II. Cardiomegalic index was decreased significantly after Herbal-Acupuncture of Daedon(LRl) Yongcheon(KI1). III. Plasma levels of atrial natriuretic peptide(ANP) was increased significantly after Herbal-Acupuncture of Taebaek(SP3) Sinmun(HT7), Daedon(LRl) Yongcheon(KIl). IV. Plasma levels of renin was decreased significantly after Herbal-Acupuncture of Taebaek(SP3) Sinmun(HT7), Daedon(LRl) Yongcheon(KI1). Conclusion : These results suggest that Blood pressure was decreased significantly after Herbal-Acupuncture of Daedon(LRl) Yongcheon(KI1) in Hypertensive RAT induced by 2K1C.

• The Korean Journal of Physiology
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• 제18권1호
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• pp.1-8
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• 1984

In order to determine the extent of the placental transfer of Lithium ion, pregnant rabbits at $27{\sim}29$ days of gestation, which has hemochorial placenta similar to the human placenta, received 2 mM/Kg of $Li^+$ in the form of LiCl intravenously. Maternal arterial blood, placental sinus blood, fetal blood, amniotic fluid and maternal urine were drawn two hours after the single dose of LiCl. Concentrations of $Li^+$, $Na^+$, $K^+$ and osmolarity were measured in plasma of collected bloods, amniotic fluid and urine. Followings are the results obtained. 1) Evident level of $Li^+$ was detected in fetal blood, although fetal plasma concentration of $Li^+$ found to be almost one third of maternal plasma. 2) Plasma concentration of $Li^+$ in placental sinus blood was higher than that in fetal plasma but lower than that in maternal plasma. It means that downward concentration gradient of $Li^+$ from mother to fetus was still remarkable two hours after the injection. 3) Significant level of $Li^+$ was also detected in amniotic fluid. It seemed likely that $Li^+$, at least in part, excreted by the fetal urinary tract. 4) There were no differences in $Na^+$ and osmolar concentration between fetal and maternal blood. 5) From above results, it was concluded that $Li^+$ may transfer across the placenta but limited passage capacity through placental barrier for $Li^+$ is significant, beacause net transfer assumed to be going on even at two hours, at which time maternal equlibrium has been reached.

• 약학회지
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• 제44권3호
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• pp.245-250
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• 2000

Because nonsteroidal anti-inflammatory drugs are reported to cause fluid retention and hypertension by inhibition of prostaglandin synthesis, the effects of piroxicam on pharmacodynamics and pharmacokinetics of nifedipine were studied in male spontaneously hypertensive rats. They received nifedipine (0.5 mg/kg) alone or combined with piroxicam (5 mg/kg) intravenously. Plasma levels norepinephrine, an index of sympathetic stimulation, were measured prior to each treatment and 5 min after drug administration. Changes in blood pressure were examined serially and blood samples for analysis of nifedipine were also taken for 6 hr following drug administration. Plasma nifedipine concentration were assayed by HPLC and pharmacokinetic parameters were calculated. Blood pressure was reduced (p<0.01), but plasma norepinephrine level was increased (p<0.05) by nifedipine administration. Anti-hypertensive effect of nifedipine was potentiated (p<0.05) by piroxicam coadministration, but effect of nifedipine on plasma norepinephrine level was not affected. In case of rats received nifedipine and piroxicam, plasma nifedipine concentrations were higher (p<0.05) than those from rats received nifedipine alone at 2,3,4,5 and 6 hours following drug administration. The area under the plasma concentration vs. time curve was increased (p<0.05), while the elimination rate constant was decreased (p<0.01) by piroxicam coadministration. No significant differences were observed in the plasma clearance, apparent volume of distribution and elimination half-life. Thus, piroxicam not only potentiated antihypertensive effect of nifedipine, but also altered nifedipine pharmacokinetics in the rats. It is concluded that the potentiation of nifedipine antihypertensive effect might correlate with the increment of its plasma concentration by piroxicam coadministration.

• 대한한의학회지
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• 제25권3호
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• pp.99-104
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• 2004

Objectives : To study the Live Blood Condition of chronic shoulder measurement. Methods : Twenty-one patients were studied. The observation items of the Live Blood Condition are the form's abnormality and cohesion of red blood cells, the abnormality of hemoglobin and the toxicity in plasma. Results : 1. The form's abnormality and cohesion of red blood cells observed were erythrocyte aggregation (8 persons), Rouleau (3 persons), target cells (7 persons), ovalocytes (3 persons), poikilocytes (2 persons). Double conditions observed were erythrocyte aggregation & target cells (3 persons), erythrocyte aggregation & poikilocytes (1 person), target cells & ovalocytes (l person). 2. The abnormality of hemoglobin and the toxicity in plasma observed were cholesterol crystals (5 persons), atherosclerotic plaque (3 persons), chylous (6 persons). 3. The form normality of red blood was generally observed in one woman. The normality of hemoglobin and the non-toxicity in plasma were generally observed in seven women. Conclusions : According to this study of the Live Blood Condition of women's shoulder measurement, these results suggest that the twenty-one patients evidence the conditions of extravasated blood and phlegm.

• The Korean Journal of Physiology
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• 제9권1호
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• pp.33-37
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• 1975

Adult rabbits were anesthetized with nembutal, 30 mg/kg. Carotid artery and jugular vein were exposed surgically and cannulated with polyethylene tubing. Arterial blood pressure was recorded via pressure transducer on the physiograph and $100{\mu}g/ml$ of histamine solution was infused through the jugular vein by using the constant infusion pump with a rate of 0.92 ml/min or 1.40 ml/min. Mean arterial blood pressure was maintained at $40{\sim}70 mmHg$ and hypotension was kept for 2 hours. After the termination of this period, blood was taken and osmotic fragility was mea sured immediately. Also, every sample of normal blood and shocked blood was incubated for 1 hour or 2 hours at $37^{\circ}C$ in order to see whether or not there was some influence of incubation. Furthermore to clarify which component was responsible for the change on the fragility, the mixtures of normal blood cells with shocked plasma and shocked blood cells with normal plasma were also incubated at $37^{\circ}C$ for one or two hours and fragility in such cases was measured. The data obtained were analysed by probit-plot method and the concentration of saline solution at which the hemolysis started to occur, 50% of blood cells were hemolysed and that at which the red blood cells hemolysed completely were determined. The values for the blood of hypotension stage were compared with those of the control blood. The results obtained were as fellows: 1. Osmotic fragility of red blood cell was increased in hypotensive state induced by histamine. 2. The differences of osmotic fragility after two hours of incubation were negligible both in normal blood and in that of hypotensive state. 3. Osmotic resistance of normal red blood cell incubated in shock plasma was less than that of shock red blood cell incubated in normal plasma. It was suggested that plasma in hypotensive state caused by histamine might be primarily responsible for the alteration of red blood cell fragility.

• Journal of Nutrition and Health
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• 제27권2호
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• pp.109-117
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• 1994

This study was conducted to evaluate dietary fat intake and its effect on the plasma lipids and fatty acids composition in plasma and red blood cells(RBC) in 96 healthy Korean female college student. Three-day food intakes were recorded, and fasting blood samples were collected and analyzed for plasma total cholesterol and triglyceride. Fatty acid compositions were determined in plasma and RBC membrane. Oleic acid was the most abundant in diet, followed by palmitic and linoleic acids. Mean daily intake of cholesterol was 219$\pm$127mg, mean plasma cholesterol was 160$\pm$24mg/이 and mean plasma triglyceride was 68$\pm$25mg/dl. Plasma fatty acids were mostly composed of linoleic, palmitic and oleic acids, while palmitic, stearic and arachidonic acids were high in RBC membrane. Plasma triglyceride showed positive correlation with BMI. Among dietary fatty acids, arachidonic acid, EPA and DHA showed negative correlation with plasma total cholesterol. Plasma triglyceride levels were negatively correlated with dietary arachidonic acid, plasma n-6 fatty acids and plasma polyunsaturated fatty acids. Dietary EPA and DHA levels were positively correlated with plasma EPA, dietary n-3/n-6 ratio were positively correlated with plasma n-3 fatty acids and n-3/n-6 ratio. Highly significant correlations were shown between the levels in plasma and RBC for several fatty acids.

• 대한수의학회지
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• 제43권4호
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• pp.641-648
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• 2003

Changes of plasma DNA contents and serum biochemical values were measured in rats administered with $HgCl_2$ to investigate the in vivo cytotoxic effects of mercury and examine the usefulness of these changes as indicators of mercury exposure and diagnosis of mercury poisoning. Rats were given once intraperitonealy $HgCl_2$(0.13. 0.32. 0.8 and 2 mg/kg b.w) and the changes of plasma DNA contents and serum biochemical values were measured at the time of 2, 4, 8, 24, 48 and 72 hours after the administration of $HgCl_2$. Plasma DNA contents began to increase from 2 hours after the administration of $HgCl_2$ in all the treatment groups significantly compared to control with dose-dependent pattern. The levels of plasma DNA reached to peak at 48 hours as 2.77, 7.60, 15.46 and 16.51 times higher than control in each treatment group of 0.13, 0.32, 0.8 and 2 mg/kgb.w, respectively and remained to be higher until 72 hours after the administration. The values of creatine kinase, aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, blood urea nitrogen and glucose of serum were increased, however the values of alkaline phosphatase, total protein and triglyceride were decreased. These changes of increase and decrease showed dose-dependent pattern but the starting time, maintenance and magnitude of change were various and characteristic according to serum biochemical indices. Among the changes of serum biochemical values, those of aspartate aminotransferase, lactate dehydrogenase and blood urea nitrogen were apparently and significantly increased compared to control from 2 to 72 hours by the administration of 2 mg/kg $HgCl_2$. This study demonstrates that plasma DNA and serum biochemical values such as aspartate aminotransferase, lactate dehydrogenase, blood urea nitrogen and etc. are valuable as biomarkers for mercury exposure assessment and diagnosis of mercury poisoning.