Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
권호 표지

Effects of HgCl2 on plasma DNA content and blood biochemical values in rats

랫드에서 수은이 혈장 DNA와 혈액화학치에 미치는 영향

  • Cho, Joon-Hyoung (National Veterinary Research and Quarantine Service) ;
  • Jeong, Sang-Hee (National Veterinary Research and Quarantine Service) ;
  • Kang, Hwan-Goo (National Veterinary Research and Quarantine Service) ;
  • Yun, Hyo-In (College of Veterinary Medicine, Chungnam National University)
  • Accepted : 2003.11.25
  • Published : 2003.12.25
Download PDF
⟨ Previous Next ⟩

Abstract

Changes of plasma DNA contents and serum biochemical values were measured in rats administered with $HgCl_2$ to investigate the in vivo cytotoxic effects of mercury and examine the usefulness of these changes as indicators of mercury exposure and diagnosis of mercury poisoning. Rats were given once intraperitonealy $HgCl_2$(0.13. 0.32. 0.8 and 2 mg/kg b.w) and the changes of plasma DNA contents and serum biochemical values were measured at the time of 2, 4, 8, 24, 48 and 72 hours after the administration of $HgCl_2$. Plasma DNA contents began to increase from 2 hours after the administration of $HgCl_2$ in all the treatment groups significantly compared to control with dose-dependent pattern. The levels of plasma DNA reached to peak at 48 hours as 2.77, 7.60, 15.46 and 16.51 times higher than control in each treatment group of 0.13, 0.32, 0.8 and 2 mg/kgb.w, respectively and remained to be higher until 72 hours after the administration. The values of creatine kinase, aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, blood urea nitrogen and glucose of serum were increased, however the values of alkaline phosphatase, total protein and triglyceride were decreased. These changes of increase and decrease showed dose-dependent pattern but the starting time, maintenance and magnitude of change were various and characteristic according to serum biochemical indices. Among the changes of serum biochemical values, those of aspartate aminotransferase, lactate dehydrogenase and blood urea nitrogen were apparently and significantly increased compared to control from 2 to 72 hours by the administration of 2 mg/kg $HgCl_2$. This study demonstrates that plasma DNA and serum biochemical values such as aspartate aminotransferase, lactate dehydrogenase, blood urea nitrogen and etc. are valuable as biomarkers for mercury exposure assessment and diagnosis of mercury poisoning.

Keywords

References

  1. Adhikari, N., Sinha, N. and Saxena, D. K. Effect of lead on Sertoli-germ cell coculture of rat. Toxicology Letters. 2000, 116, 45-49
  2. Bret, L., Lule, J., Alary, C. Appolinaire-Pilipenko, S., Pourrat, J. P. and Fournie, G. J. Quantitation of blood plasma DNA as an index of in vivo cytotoxicity. Toxicology. 1990, 61, 283-292
  3. Brown, A. P., Morrissey, R. L., Faircloth, G. T. and Levine, B. S. Preclinical toxicity studies of kahalalide F, a new anticancer agent: single and multiple dosing regimens in the rat. Cancer Chemother Pharmacol. 2002, 50, 333-340
  4. Cantoni, O. and Costa, M. Correlation DNA strand brakes and their repair with cell survival followung acute exposure to mercury(II) and X-rays. Molecular pharmacology. 1983, 24, 84-89
  5. Cantoni, O., Christie, N. T., Swann, A. Drath, D. B. and Costa, M. Mechanism of $HgCl_2$ cytotoxicity in mammalian cells. Molecular Pharmacology. 1984, 26, 360-368
  6. Cardenas, A., Roels, H., Bernard, A. M. Barbon, R., Buchet, J. R, Rosello, J., Ramis, I., Mutti, A. and Franchini, I. Markers of early renal changes induced by industrial pollutants, I Application to workers exposed to mercury vapour. British Journal of Industrial Medicine. 1993, 50, 17-27
  7. Fischer, A. B. and Skreb, Y. Cytotoxicity of manganese for mammalian cells in vitro-comparisons with lead, mercury and cadmium. Zentralbl Bakteriol Mikrobiol Hyg[B], 1980, 171, 525-537
  8. Fournie', G. J., Gayral-Taminh, M., Bouche, J. P. Recovery of nanogram quantities of DNA from plasma and quantitative measurement using labeling by nick translation. Analytical Biochemistry. 1986, 158, 250-256
  9. Gritzka, T. L. and Trump, B. F. Renal tubular lesions caused by mercuric chloride. Am. J. Pathol. 1968, 52, 1255-1277
  10. Kasatori, N., Urayama, T., Mori, T. and Ishikawa, F. Cytotoxicity test based on luminescent assay of alkaline phosphatase released from target cells. Rhinsho Byori. 1994, 42, 1050-1054
  11. McQueen, C. A. and Williams, G. M. Cytotoxicity of genobiotics in adult rat hepatocytes in primary culture. Fundam. Appl. Toxicol. 1982, 2, 139-144
  12. Natori, Y., Igawa, Y. and Nakao, N. Cytotoxicity of sera from rats with puromycin aminonucleoside nephrosis. Nephron. 1996, 73, 258-263
  13. Nieminen, A. L., Gores, G. J., Bond, J. M. Imberti, R., Herman, B. and Lemasters, J. J. A novel cytotocixity screening assay using a multiwell fluorescence scanner. Toxicology and Applied Pharma-cology. 1992, 115, 147-155
  14. Obatomi, D. K. and Bach, P. H. Atractyloside nephro-toxicity: in vivo studies with suspensions of rat renal fragments and precision-cut cortical slices, In Vitr Mol Toxicol. 2000, 13, 25-36
  15. Pearson, A. W. Biochemical changes produced by Fusarium T-2 toxin in the chicken. Res. Vet. Sci. 1978, 24, 92-97
  16. Peters, M. M., Jones, T. W., Monks, T. J. and Lau, S. S. Cytotoxicity and cell-proliferation induced by the nephrocarcinogen hydroquinone ad its nephrotoxic metabolite 2,3,5-(tris-glutathion-S-yl)hydroquione. Car-cinogenesis. 1997, 18, 2393-2401
  17. Roles, H., Gennart, J. R, Lauwerys, R. Buchet, J. P., Malchaire, J. and Bernard, A. Surveillance of workers exposed to mercury vapor: validation of a previously proposed biological threshold limit value for mercury concentration in urine. Am. J. Ind. Med. 1985, 7, 45-71
  18. Svendsen, O., Hojelse, F. and Bagdon, R. E. Tests for local toxicity of intramuscular drug preparations: comparison of in vivo and in vitro methods. Acta Pharmacol. Toxicol. 1985, 56, 183-190
  19. Takeuchi, T., Sugimoto, T., Nishida, N. and Kobayashi, Y. Evaluation of cytotoxicity of sodium valproate on primary cultured rat hepatocytes. Neuropediathcs. 1988, 19, 158-161
  20. Traore, A., Bonini, M., Dano, S. D. and Creppy, E. E. Synergistic effects of some metals contaminating mussels on the cytotoxicity of the marine toxin okadaic acid. Arch. Toxicol. 1999, 73, 289-295
  21. Wailiams, M. V., Winters, T. and Waddell, K. S. In vivo effects of mercury(II) on deoxyuridine triphosphate nucleo-tidohydrolase, DNA polymerase($\alpha$,$\beta$) and uracil-DNA glycosylase activities in cultured human cells: relationship to DNA damage, DNA repair and cyto-toxicity. Molecular phannacology. 1987, 31, 200-207