• Journal of Nutrition and Health
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• 제36권9호
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• pp.889-897
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• 2003

This study was carried out to determine the effect of dietary ${\gamma}$-linolenic acid on decreasing the plasma lipid levels and the thrombotic activity in rats. Sprague-Dawley male rats (B.W 120 g) were fed a experimental diet containing 5% lard (46.05% saturated fatty acids) , corn oil (51.36% linoleic acid) , evening primrose oil (EPO,72.80% linoleic acid and 9.16% ${\gamma}$-linolenic acid) or borage oil (BO,40.29% linoleic acid and 24.25% ${\gamma}$-liolenic acid) for 30 days. Although there were no significant differences in the food intake among the groups, the body weight gain of the BO group was significantly lower than that of the other groups. The bleeding time of the BO group was significantly longer than that of the other groups. There were significantly differences in the whole blood clotting time among the groups except for the EPO and corn oil groups, where the whole blood clotting time of the BO group was the highest among the groups, and that of the lard group was the lowest. The plasma triacyglyceride (TAG) , total cholesterol (TC) and low density lipoprotein cholesterol (LDL-C) concentrations were the lowest in the BO group, but highest in the lard group, and there were significant differences among the groups. The plasma HDL-C concentrations were in the following order: BO, EPO, corn oil and lard groups and there were significant differences among the groups. The excretions of fecal neutial steroids and acidic steroids of the BO group were the highest among the groups, and there were significant differences compared to the other groups. The results suggest that dietary EPO and BO containing ${\gamma}$-linolenic acid has an antithrombotic activity, and inhibits the increasing of plasma TAG, TC and LDL-C concentrations compared to lard, which contains saturated fatty acids, or corn oil, which contains linoleic acid.

• Journal of Nutrition and Health
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• 제28권10호
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• pp.995-1003
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• 1995

This study was conducted to asses the $\omega$-3 fatty acid intake levels and to evaluate the relationship between the levels of $\omega$-3 fatty acids in plasma and erythrocyte membrane and the dietary intake of these fatty acids over the period of two months in 56 young Korean females. Dietary survey was conducted to obtain 7-day weiged records and six 24-hour recalls. Fasting blood sample was collected from each subject after the dietary survey period. Mean daily intakes of energy, fat, and cholesterol were 1,569㎉, 41.8g, and 217mg, respectively. Fat suppled 24% of total energy intake. Mean daily intake levels of eicosapentaenoic acid(EPA), docosahexaenoic acid(DHA), and total $\omega$-3 fatty acids were 0.04g, 0.06g, and 0.48g, respectively. Plasma cholesterol levels of most of the subjects were within normal range, and there was no significant correlation between plasma cholesterol levels and intake levels of any specific fatty acid. Levels of EPA, DHA, EPA+DHA in plasma and erythrocyte membrane, but not the levels of $\alpha$-linolenic acid(LNA), were significantly correlated with dietary in take of respective fatty acids. Such a correlation, however, was not observed in a previous study where dietary intake was assessed for 3 days. The results of this study show that dietary intakes of $\omega$-3 fatty acids are low in the subject and that about two-month period is required to assess dietary intake levels of $\omega$-3 fatty acids with a reasonable accuracy.

• 대한지역사회영양학회지
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• 제9권1호
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• pp.58-65
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• 2004

The purpose of this research was to examine the relationship between the plasma LDL particle size and blood lipid profile, dietary factors and anthropometric values (body mass index, waist circumference and waist/hip ratio). The subjects were 173 adults aged 23 to 81 years, selected from the Outpatient Clinic and Cardiovascular Department of the Seoul Municipal Hospital. Dietary data were obtained using a 3-day food record and analyzed using Korean and US nutrient databases. The subjects were divided into three groups by LDL particle size : type A (large buoyant LDL, > 25.5 nm, n=96), type I (Intermediate LDL,$25.2\leq-\leq25.5$ nm, n=18), and type B (small dense LDL, < 25.2 nm, n=59) groups. The type B group had higher age, waist circumference, and waist/hip ratio (WHR) than the type A and type I groups. Serum concentration of triglyceride, Apo B, LDL/HDL cholesterol ratio and atherogenic index were significantly higher in the type B group as compared to those in the other two groups. HDL cholesterol level and Apo A-I/Apo B ratio were significantly lower in the type B group than the other two groups. The plasma LDL particle size was highly correlated with triglyceride (r= -0.450), Apo B (r= -0.402) and HDL cholesterol (r= 0.418). However, there was no correlation between plasma LDL particle size and dietary intakes. This study showed that small dense LDL was an important biochemical risk factor that was associated with other risk factors.

• The Korean Journal of Physiology
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• 제14권1호
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• pp.7-13
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• 1980

In the previous experiment, authors have shown that during the latter half of estrous cycle there was an increase in plasma testosterone level in the rats stimulated with hCG. To determine the physiologic significance of elevated plasma testosterone, changes of the plasma concentrations of TeBG and testosterone following hCG stimulation were analyzed in the rats having a regular 5 day cycle. The rats were divided into three groups; the control, the rats stimulated with single hCG on the day of proestrus and stimulated with hCG throughout the entire cycle. Blood samples were obtained once a day for an estrous cycle and analyzed for the binding capacity of TeBG using ammonium sulphate precipitation method and testosterone concentration by means of radioimmunoassay. Followings were the results; 1) There was no significant variation in the binding capacity of TeBG in peripheral blood during the estrous cycle of the control rats. 2) No cyclic variation in the binding capacity of TeBG was observed in the rats stimulated with single hCG on proestrus. although the levels tended to be higher in the rats with stimulation than in the control rats. 3) Continual stimulation of hCG produced a marked increase in the binding capacity of TeBG especially on the day of metaestrus. 4) The changes in the plasma level of testosterone followed the same basic pattern seen in the TeBG binding capacity. 5) From above results, the followings were suggested. a. hCG related increase of the binding capacity of TeBG is probably secondary to a modest increase in estrogen as well. b. hCG related increase of plasma testosterone in female rats is not entirely due to excess production rather in part due to decreased metabolism induced by the rise in TeBG. c. It seems likely that most of elevated testosterone shown in the rat stimulated with hCG is bound to TeBG and only small portion is unbound form which influence cellular activity. It is rather possible that an increase in TeBG could augment estrogen activity.

• Journal of Pharmaceutical Investigation
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• 제39권6호
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• pp.423-429
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• 2009

Liposomes have been used as one of the efficient carriers for drug delivery. In this study, anionic liposomes of which surface was modified by using both electrostaic interaction between anionic liposomes and cationically charged BSA molecules at lower pH than isoelectric point (pI) of BSA and denaturation of the BSA-coated liposomes by thermal treatment. The thermally denatured BSA-coated liposomes (DBAL) had mean particle diameter of 125.2${\pm}$1.7 nm and zeta potential value of -22.4${\pm}$4.5 mV. Loading efficiency of model drug, doxorubicin (DOX), into liposomes was 83.0${\pm}$2.6%. Results of in vitro stability study of DBAL in blood plasma showed that the mean particle diameter of DBAL 400 did not increase in blood plasma and adsorption of plasma protein was much less than plain or anionic liposomes. Intracellular uptake of DBAL 400 evaluated by confocal microscopy observation was higher than that of PEG liposomes.

• 한국양식학회지
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• 제9권1호
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• pp.3-10
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• 1996

인간의 태반성 성선자극호르몬(HCG) 또는 성선자극호르몬-방출호르몬 유도체(GnRH-A)와 pimozide를 이용하여 산란시기의 암컷황복에서 인공 산란을 유도하였다. 호르몬 또는 호르몬유도체의 효과는 배란 후 인공수정시켜 수정율과 배체형성율 및 면역방사측정법으로 혈액의 plasma 내 성선자극호르몬(GTH) 양을 측정하여 결정하였다. 수정율과 배체형성율이 가장 좋은 HCG 농도는 어체중 kg 당 1,000 IU이었다. 또한 GnRH-A ($10\;{\mu}g/kg$)와 pimozide를 동시에 처리했을 때 수정율과 배체형성율이 좋은 pimozide의 농도는 5 mg/kg이었다. Pimozide(1, 5 mg/kg)를 단독으로 처리한 어류에서는 plasma 내 GTH양에 영향을 줄 수 없었지만, GnRH-A와 동시에 처리했을 때 GTH양은 현저히 증가되었다. GnRH-A와 pimozide 및 dopamine을 동시에 처리했을 때 혈액의 plasma 내 GTH양은 크게 감소되었다. 이상의 결과는 산란시기 또는 이시기이외에 황복의 뇌로부터 여러 가지 성선자극호르몬과 성선자극억제호르몬을 분비됨을 시사해 준다.

• Journal of Korean Neurosurgical Society
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• 제59권5호
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• pp.442-448
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• 2016

Objective : Increased expression of angiogenic factors, such as vascular endothelial growth factor (VEGF), is associated with the pathogenesis of cerebral cavernous malformations (CCMs). The purpose of this study was to investigate plasma levels of VEGF in normal subjects and in patients with CCM and to evaluate change in these levels following stereotactic radiosurgery (SRS). Methods : Peripheral venous blood was collected from 6 patients with CCM before SRS using Gamma Knife and at the 1 week, 1 month, 3month, and 6 month follow-up visits. Plasma VEGF levels were measured using commercially available enzyme-linked immunosorbent assay kits. Peripheral blood samples were obtained from 10 healthy volunteers as controls. Results : Mean plasma VEGF level of 41.9 pg/mL (range, 11.7-114.9 pg/mL) in patients with CCM at baseline was higher than that of the healthy controls (29.3 pg/mL, range, 9.2-64.3 pg/mL), without significant differences between CCM patients and controls (p=0.828). Plasma VEGF level following SRS dropped to 24.6 pg/mL after 1 week, and decreased to 18.5 pg/mL after 1 month, then increased to 24.3 pg/mL after 3 months, and 32.6 pg/mL after 6 months. Two patients suffering from rebleeding after SRS showed a higher level of VEGF at 6 months after SRS than their pretreatment level. Conclusion : Plasma VEGF levels in patients with CCM were elevated over controls at baseline, and decreased from baseline to 1 month after SRS and increased further for up to 6 months. Theses results indicated that anti-angiogenic effect of SRS might play a role in the treatment of CCMs.

• Journal of Nutrition and Health
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• 제26권7호
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• pp.899-906
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• 1993

사람의 혈중 셀레늄의 농도는 식품의 셀레늄 함유량과 생리적 이용도에 따른다. 그러므로 특히 토양중 셀레늄의 농도가 낮은 나라에서는 식이 형태에 따라 셀레늄의 섭취량과 혈중 셀레늄 농도의 차이를 볼 수 있다. 본 실험에서는 평균 독일 식이(average German diet)를 하는 건강한 성인 여자 175명과 wholesome nutrition을 지켜온 243명을 대상으로 셀레늄의 영양상태를 살펴보았다. 평균 독일 식이군에서 평균 전혈 셀레늄의 농도와 혈장 셀레늄의 농도는 84.79$\mu\textrm{g}$/l, 혈장 셀레늄의 농도는 75.02$\mu\textrm{g}$/l 이었고, wholesome nutrition군에서 전혈 셀레늄의 농도와 혈장 셀레늄의 농도가 각각 78.62$\mu\textrm{g}$/l 와 63.95$\mu\textrm{g}$/l로 두 식이군 간에 전혈 셀레늄 농도와 혈장 셀레늄 농도에서 모두 유의적인 차이를 나타내었다. 그리고 셀레늄의 혈중 농도에 영향을 주는 요인을 알아보기 위하여 BMI, 나이, 하루 식품 섭취량과의 상관 관계를 조사해 보았다. BMI와 나이는 전혈, 그리고 혈장 셀레늄 농도와 유의적인 상관관계를 찾아볼 수 없었으며, 하루 식품 섭취량과의 상관 관계에서는 wholesome nutrition군에서 전혈 셀레늄 농도에서 모두 생선 섭취량과 유의적인 상관 관계를 나타내었다.

• 한국식품과학회지
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• 제35권2호
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• pp.280-285
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• 2003

본 연구에서는 streptozotocin-유도 당뇨쥐를 이용하여 배양인삼의 첨가식이가 혈당강하 및 지질대사 등에 미치는 영향을 조사하기 위해 대조군과 당뇨대조군, 5% 및 10% 배야인삼첨가군 등 모두 4군으로 나누어 해당식이로 2주간 사육하였다. 배양인삼은 당뇨쥐의 체중증가와 혈당저하에 효과를 나타냈으며, 수분 및 사료 섭취량도 줄여 당뇨병 증세의 개선효과를 보였다. 또한 당뇨쥐의 지질대사를 개선하여 혈장중성지방, 총 콜레스테롤 및 동맥경화지수를 저하시키고 총 콜레스테롤에 대한 HDL-콜레스테롤의 비율을 증가시켰다. AST 및 ALT의 활성은 5% 첨가군에서만 당뇨대조군에 비해 낮은 값을 보였으며, 10% 첨가군에서는 오히려 높은 값을 보였다. Creatinine의 함량은 모든 첨가군에서 당뇨대조군에 비해 현저히 낮은 값을 보였다.

• Reproductive and Developmental Biology
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• 제37권4호
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• pp.247-253
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• 2013

Here, we present an approach of blood plasma proteome profiling and their comparisons between the young and the adult pigs as prerequisite for the identification of bio-markers related to the health conditions, growth performance and meat quality. To profile the proteome in porcine plasma, blood samples were collected from 19 young piglets and 20 adult male barrows and the plasma was retrieved. Then, protein profiling was initiated using one and two-dimensional electrophoresis. Proteins were spotted and then identified by MALDI-TOF-TOF and LC-MS-MS. In the results, more than thirty-six and twenty eight protein spots were selected in young piglets and adult pigs, respectively and twenty three proteins were identified. The proteome profile images were compared between those ones using Image Master Version 7.0. The image of expressed proteome showed that most of proteins from plasma of young piglet separated clearly and concentrated in 2DE display compared to ones from adult. Image analysis in detail was carried out to look for the specific proteins related to age progression. It demonstrated that the characteristics of proteome expression could be distinct to their age stages. Further investigations needed to proceed to understand the age dependent change of protein conformation and biological meaning of those differences in proteome expression between young and mature adult pigs.