Journal of Pharmaceutical Investigation

The Korean Society of Pharmaceutical Sciences and Technology (한국약제학회)
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Stability in Plasma and Intracellular Uptake of Thermally Denatured Protein-coated anionic Liposomes

열변성 단백질이 결합된 음이온성 리포솜의 혈장 내 안정성 및 세포 내 이입 평가

  • Lee, Mi-Jung (Biomaterials Research Center, Korea Research Institute of Chemical Technology,Department of Pharmaceutical Science, College of Pharmacy, Kyung Hee University) ;
  • Hwang, In-Young (Biomaterials Research Center, Korea Research Institute of Chemical Technology,Department of Pharmaceutical Science, College of Pharmacy, Kyung Hee University) ;
  • Kim, Sung-Kyu (Biomaterials Research Center, Korea Research Institute of Chemical Technology) ;
  • Jung, Suk-Hyun (Biomaterials Research Center, Korea Research Institute of Chemical Technology) ;
  • Jeong, Seo-Young (Department of Pharmaceutical Science, College of Pharmacy, Kyung Hee University) ;
  • Seong, Ha-soo (Biomaterials Research Center, Korea Research Institute of Chemical Technology) ;
  • Cho, Sun-Hang (Biomaterials Research Center, Korea Research Institute of Chemical Technology) ;
  • Shin, Byung-Cheol (Biomaterials Research Center, Korea Research Institute of Chemical Technology)
  • 이미정 (한국화학연구원 바이오소재연구센터,경희대학교 약학대학) ;
  • 황인영 (한국화학연구원 바이오소재연구센터,경희대학교 약학대학) ;
  • 김성규 (한국화학연구원 바이오소재연구센터) ;
  • 정석현 (한국화학연구원 바이오소재연구센터) ;
  • 정서영 (경희대학교 약학대학) ;
  • 성하수 (한국화학연구원 바이오소재연구센터) ;
  • 조선행 (한국화학연구원 바이오소재연구센터) ;
  • 신병철 (한국화학연구원 바이오소재연구센터)
  • Published : 2009.12.20
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Abstract

Liposomes have been used as one of the efficient carriers for drug delivery. In this study, anionic liposomes of which surface was modified by using both electrostaic interaction between anionic liposomes and cationically charged BSA molecules at lower pH than isoelectric point (pI) of BSA and denaturation of the BSA-coated liposomes by thermal treatment. The thermally denatured BSA-coated liposomes (DBAL) had mean particle diameter of 125.2${\pm}$1.7 nm and zeta potential value of -22.4${\pm}$4.5 mV. Loading efficiency of model drug, doxorubicin (DOX), into liposomes was 83.0${\pm}$2.6%. Results of in vitro stability study of DBAL in blood plasma showed that the mean particle diameter of DBAL 400 did not increase in blood plasma and adsorption of plasma protein was much less than plain or anionic liposomes. Intracellular uptake of DBAL 400 evaluated by confocal microscopy observation was higher than that of PEG liposomes.

Keywords

References

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