• Title/Summary/Keyword: Blood plasma

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Effects of Yarrowia lipolytica supplementation on growth performance, intestinal health and apparent ileal digestibility of diets fed to nursery pigs

  • Cheng, Yi-Chi;Duarte, Marcos Elias;Kim, Sung Woo
    • Animal Bioscience
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    • v.35 no.4
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    • pp.605-613
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    • 2022
  • Objective: The objective was to evaluate the efficacy of increasing supplementation of Yarrowia lipolytica (YL) up to 3.0% replacing 1.6% poultry fat and 0.9% blood plasma for growth performance, intestinal health and nutrient digestibility of diets fed to nursery pigs. Methods: Twenty-four pigs weaned at 24 d of age (initial body weight at 7.2±0.6 kg) were allotted to three dietary treatments (n = 8) based on the randomized complete block. The diets with supplementation of YL (0.0%, 1.5%, and 3.0%, replacing poultry fat and blood plasma up to 1.6% and 0.9%, respectively) were fed for 21 d. Feed intake and body weight were recorded at d 0, 10, and 21. Fecal score was recorded at every odd day from d 3 to 19. Pigs were euthanized on d 21 to collect proximal and distal jejunal mucosa to measure intestinal health markers including tumor necrosis factor-alpha, interleukin-8, immunoglobulin A and immunoglobulin G. Ileal digesta was collected for apparent ileal digestibility (AID) of nutrients in diets. Data were analyzed using Proc Mixed of SAS. Results: Supplementation of YL (1.5% and 3.0%) replacing poultry fat and blood plasma did not affect growth performance, fecal score and intestinal health. Supplementation of YL at 1.5% did not affect nutrient digestibility, whereas supplementation of YL at 3.0% reduced AID of dry matter (40.2% to 55.0%), gross energy (44.0% to 57.5%), crude protein (52.1% to 66.1%), and ether extract (50.8% to 66.9%) compared to diets without supplementation. Conclusion: Yarrowia lipolytica can be supplemented at 1.5% in nursery diets, replacing 0.8% poultry fat and 0.45% blood plasma without affecting growth performance, intestinal health and nutrient digestibility. Supplementation of YL at 3.0% replacing 1.6% poultry fat and 0.9% blood plasma did not affect growth performance and intestinal health, whereas nutrient digestibility was reduced.

Effect of Intravascular Laser Irradiation on Lipoprotein(a) (저용량 He-Ne 레이저 정맥내(靜脈內) 조사(照射)가 혈중(血中) Lipoprotein(a)에 미치는 영향(影響))

  • Lee, Dong-Joon;Sun, Jung-Ki;Choi, Chang-Won;Lim, Jin-Hoon
    • The Journal of Internal Korean Medicine
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    • v.21 no.5
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    • pp.839-844
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    • 2000
  • Objectives : The purpose of study was to investigate the effect of ILlB(intravascular laser irradiation of blood) on serum lipoprotein(a) Methods : The 12 case is diagnosed as cerebrovascular diseases on computed tomograghy. The 18 case is not observed abnormality on computed tomograghy but, they have neurological sign. Blood test was enforced on the first medical examination, The 15 case with plasma lipoprotein(a) greater than 30 mg/dl have been classified into abnormal group and the 15 case of less than 30 mg/dl have been classified into control group. after ten times ILlB, It was observed that the change of plasma lipoprotein(a) and a fibrinogen of blood clotting factor. Conclusions : 1. After ten times ILlB, plasma lipoprotein(a) numerical value was decreased on 13 case of abnormal group. 2. Plasma lipoprotein(a) numerical value was decreased on 5 case of control group. 3. The twenty in fifteen patient's with hyperlipoproteinemias have been fibrinogenemias and after ILIB treatment, in the all of 16 case with hyperfibrinogenemia in the first medical examination to both abnormal and control group, plasma fibrinogen numerical value was decreased.

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Changes in Plasma Lipid Pattern in Streptozotocin-Induced Diabetic Rats: A Time Course Study (스트렙토토신-당뇨쥐의 유병기간에 따른 혈중지질패턴의 경시적 변화)

  • 이수자
    • Journal of Nutrition and Health
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    • v.32 no.7
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    • pp.767-774
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    • 1999
  • This study was carrid out to examine a part of the mechanism for the etiology of diabetic complications. Thirty normal and forty streptozotocin(STZ)-induced diabetic rats were used as the animal models. Animals were sacrificed at the time points of 3 days, 1, 2, 4 and 6 weeks after STZ-injection and time course in body weight and organ weight, the levels of blood glucose, plasma lipid patterns, and atherogenic index were measured during 6 weeks. The STZ-diabetic animals showed 63% survival rate and fsting blood glucose levels of the diabetic animals measured in the range of 230-410mg/dL during the experimental period. The body weigh of diabetic animals decreased significantly throughout the experimental period and the relative weights of organs to body weight were significantly higher than the normal control ones. The enlargement of the kidney in the diabetic animals was especially remarkable. Plasma triglyceride concentration in diabetic rats substancially increased from the first week of onset of diabetes mellitus and maintained higher levels than the control ones throughout the whole experimental period. The plasma total cholesterol level and atherogenic index in the diabetic rats were significantly higher than the normal ones from the third day after STZ injection and showed a gradual increase with the duration of the disease. Throughout the experiment, the diabetic rats consistently showed a slightly lower HDL-cholesterol level compared to the normal animals. From the results of this study, it appears that the significant changes in blood lipid pattern in STZ-diabetic animals start from the first week after STZ injection.

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Characterization of Plasma Proteins from Bloods of Slaughtered Cow and Pig and Utilization of the Proteins as Adhesives (도축혈액 혈장 단백질의 특성 및 접착제로의 응용)

  • Park, Eun-Hee;Lee, Hwa-Hyoung;Song, Kyung-Bin
    • Applied Biological Chemistry
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    • v.39 no.2
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    • pp.123-126
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    • 1996
  • Simple and rapid method of purification of plasma proteins from bloods of slaughtered animals was developed and the proteins were applied to plywood products as a blood 히ue to utilize waste materials. Plasma protein was obtained by adding 2% trichloroacetic acid (TCA) or 0.6 N HCI as optimal concentration to the supernatant, after centrifugation of bloods. Molecular properties of beef and pig plasma proteins were examined on SDS-PAGE. Application of blood glue to plywood was quite satisfactory compared to the synthetic amino resin by tensile-shear test for the strength of adhesive bonding.

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Analysis of Residual Furan in Human Blood Using Solid Phase Microextraction-Gas Chromatography/Mass Spectrometry (SPME-GC/MS)

  • Lee, Yun-Kyung;Jung, Seung-Won;Lee, Sung-Joon;Lee, Kwang-Geun
    • Food Science and Biotechnology
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    • v.18 no.2
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    • pp.379-383
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    • 2009
  • For an accurate risk assessment of furan, a potential human carcinogen, levels must be determined in human blood plasma using a simple and robust assay. In this study, solid phase microextraction-gas chromatography/mass spectrometry (SPME-GC/MS) was used to analyze blood plasma levels of furan in 100 healthy individuals who consumed a normal diet. The subjects were 30 to 70 years of age and 51% were women. Ultimately, an analytical method was established for analyzing furan in human blood. The limit of quantification (LOQ) and furan recovery rate in blood were 1.0 ppb and 104%, respectively. Finally, furan was detected in 21 individuals (13 males, 8 females) with levels ranging up to 17.86 ppb (ng furan/g food).

Experimental Studies on the Effect of Qingbaozhuyutang(?胞逐瘀湯), Bee Veenom on the Blood Stasis Pattern (청포축어탕 및 봉독(蜂毒)이 어혈병태모형(瘀血病態模型)에 미치는 영향(影響))

  • Gang, Sun-Hee;Choi, Seung-Hoon;Ahn, Kyoo-Seok
    • The Journal of Korean Medicine
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    • v.16 no.1 s.29
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    • pp.351-369
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    • 1995
  • In order to investigate the effect of Qingbaozhuyutang(淸胞逐瘀湯), Bee Veenom on 'Blood Stasis Pattern', here We regard Thrombosis and Elevated Blood viscosity as Blood Stasis Pattern. rats were injected Endotoxin into the caudal vein to make experimental thrombosis model(control group), and at sample group, rats were orally administrated solid extract of Qingbaozhuyutang(淸胞逐瘀湯) 0.5g/200g or were injected Apitoxin 1mg/Kg into the abdominal region(sample group), and then we observed platelet, fibrinogen, prothrombin time and FDP level. Another group were injected HA into the muscular rump to make experimental elevated blood viscosity instead of Endotoxin. Thereafter we measured whole blood viscosity and plasma viscosity. The results were summarized as follows. 1. Platelets were increased significantly in sample I compared with the control group. 2. Fibrinogen levels were increased significantly in sample I compared with the control group. 3. Prothrombin times were shortened significantly in sample II and sample III compared with the control group. 4. FDP levels were decreased significantly in sample II and sample III compared with the control group. 5. Whole blood viscosity and plasma viscosity were decreased significantly in all shear rates compared with the control group. According to the above results, it is considered that Qingbaozhuyutang(淸胞逐瘀湯) has significant effects on platelets, fibrinogens, prothrombin times, FDP levels, whole blood viscosity and plasma viscosities, therefore it seems to be applicable to disease related to 'Blood Stasis Pattern' through the thrombosis and elevated blood viscosity. But, Veenom has a little effect. the mechanism concerned for the effects is to be investigated in future.

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Effect of Diuresis on Plasma Renin Activity and Aldosterone Concentration in Normal and Toxemic Pregnancy (정상임부와 임신중독환자의 혈장 Renin활성도 및 Aldosterone 농도에 미치는 이뇨의 효과)

  • Sung, H.K.;Lee, H.S.;Cho, S.S.;Koh, J.H.;Lee, J.K.;Kim, H.S.
    • The Korean Journal of Nuclear Medicine
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    • v.7 no.1
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    • pp.45-50
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    • 1973
  • The changes of plasma renin activity, aldosterone concentration, serum sodium-, and potassium levels were studied before and after the water loading followed by diuretics injection. The materials were: 13 non-, 11 normal-, and 11 toxemic pregnancy cases. The plasma renin activity and aldosterone concentration of the cord and postpartum blood were also measured. Following were the results: 1. The plasma renin activity was elevated significantly in normal pregnancy, and slightly in toxemic pregnancy. The serum sodium levels were decreased in pregnancy. 2. The plasma aldosterone concentration was slightly decreased in normal pregnancy, and slightly increased in toxemic pregnancy, however, statistically insignificant. 3. The plasma renin activity of the cord and postpartum blood were lower than those of pregnancy cases. 4. The changes of plasma renin activity after the diuretic administration showed an initial increase, which recovered within 2 hours. These changes were the least in normal pregnancy, and the most in toxemic pregnancy. 5. The changes of plasma aldosterone concentration after the diuretic administration were similar to those of plasma renin activity, although the variations were not so wide.

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Correlations of Plasma Taurine, Homocysteine and Serum Lipid Levels of Elderly Women (노년기 여성들의 혈장 타우린의 농도와 혈장 호모시스테인 및 혈청 지질농도와의 상관관계)

  • Ahn, Chang-Soon;Lee, Yeon-Joo
    • The Korean Journal of Food And Nutrition
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    • v.23 no.4
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    • pp.501-510
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    • 2010
  • This study was conducted to assess blood components caused by metabolic syndrome increasing in postmenopausal elderly women. The blood samples of these subjects were analyzed to investigate the correlation of plasma taurine levels and plasma homocysteine levels, and serum lipid profiles. The subjects were 33 elderly women($72.8{\pm}4.4$ years). Their mean height, weight and BMI were $150.5{\pm}5.7\;cm$, $57.5{\pm}6.3\;kg$ and $25.4{\pm}2.5\;kg/m^2$. 16 women of this study subjects have been chronic diseases such as hypertension or diabetes. Their fasting blood glucose was $98.2{\pm}24.0\;mg/d{\ell}$, and their plasma total cholesterol (TC), HDL-C, LDL-C, triglyceride(TG) were $216.5{\pm}29.9$, $52.1{\pm}10.7$, $145.7{\pm}27.9$ and $141.2{\pm}59.6\;mg/d{\ell}$, respectively. Their blood lipid profiles were higher than the standard levels of metabolic syndrome, thus these levels of lipid profiles may play a role as risk factors on the elderly person. Plasma taurine level of the subjects was $278.5{\pm}48.1\;{\mu}mol/{\ell}$, and their plasma homocysteine level was $12.8{\pm}2.9\;{\mu}mol/{\ell}$. The concentration of plasma vitamin $B_{12}$ was significantly decreased by aging(p<0.05). The correlation of plasma homocysteine and plasma folate showed significantly negative(p<0.05). Thus, the decreased levels of plasma vitamin $B_{12}$ and folate by aging might affect on the plasma homocyteine concentration acting as a risk factor of cardiovascular diseases for elderly person. The correlation of plasma taurine and hemoglobin, and their platelet showed significantly positive(p<0.05). In conclusion, the diet on the elderly person is one of the important factors to prevent their health from chronic diseases. This study recommends that well balanced diets are needed for elderly person to keep their health and prevent from metabolic syndrome.

Separation of monocytes from canine peripheral blood (개 말초혈액(末稍血液)에서 monocytes 분리(分離))

  • Kim, Jeoung-bae;Lee, Bang-whan
    • Korean Journal of Veterinary Research
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    • v.29 no.2
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    • pp.33-39
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    • 1989
  • Pure separation of various leukocytes is required for the assessment of their roles in immunological and phisiological function. In this study, pure separation of monocytes from canine peripheral blood was attempted. At first, mononuclear cells (PBMC) were separated by ficoll-hypaque gradient method and then monocytes were recovered from PBMC suspensions in sucrose gradient Sol. (PBMC-Sucrose), autologous plasma (PBMC-Plasma) and autologous serum (PBMC-Serum) incubated at $37^{\circ}C$ for 2 hours. 1. In the separation of PBMC by ficoll-hypaque gradient method in canine blood, higher relative centrifugal force (RCF) was required, as high as more than 1,300xg RCF for 40 minutes, for clear formation of PBMC layer than that in human blood as usually used 400xg RCF for 40 minutes. 2. In monocytes-separation from three PBMC suspensions following PBMC separation, recovery-, purity- and viability-rate of monocytes showed better results in PBMC-Plasma and PBMC-Serum than in PBMC-Sucrose suspension, particulary showing better results from PBMC suspensions performed by centrifugation at 1,500xg RCF for 40 minutes.

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Effects of Interferents in Blood on the Blood Glucose Measurements by Using Polyphosphazene Diagnostic Membranes (포스파젠 고분자 진단막을 이용한 혈당 측정 시 혈액 중 장애성분이 미치는 영향)

  • Kwon, Suk-Ky
    • Membrane Journal
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    • v.23 no.4
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    • pp.297-303
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    • 2013
  • Diagnostic membranes which were made of polyphosphazenes were prepared for the measurements of blood glucose concentration. The effects of possible interferents in human blood on the glucose concentration measurements were examined. Above those interferents, ascorbic acid (AA) showed 3~9% higher K/S values compared to the standard plasma solution, tolbutamide (TA) showed 11~13% lower K/S values than those of plasma, triglycerides and bovine serum albumin (BSA) showed 20~25% lower K/S values than those of plasma. However, most of the interferents except above materials did not show any serious effects on the blood glucose measurements.