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Analysis of Residual Furan in Human Blood Using Solid Phase Microextraction-Gas Chromatography/Mass Spectrometry (SPME-GC/MS)  

Lee, Yun-Kyung (Department of Food Science and Technology, Dongguk University)
Jung, Seung-Won (Department of Food Science and Technology, Dongguk University)
Lee, Sung-Joon (Division of Food Science, College of Life Science and Biotechnology, Institute of Biomedical Sciences and Safety, Korea University)
Lee, Kwang-Geun (Department of Food Science and Technology, Dongguk University)
Publication Information
Food Science and Biotechnology / v.18, no.2, 2009 , pp. 379-383 More about this Journal
Abstract
For an accurate risk assessment of furan, a potential human carcinogen, levels must be determined in human blood plasma using a simple and robust assay. In this study, solid phase microextraction-gas chromatography/mass spectrometry (SPME-GC/MS) was used to analyze blood plasma levels of furan in 100 healthy individuals who consumed a normal diet. The subjects were 30 to 70 years of age and 51% were women. Ultimately, an analytical method was established for analyzing furan in human blood. The limit of quantification (LOQ) and furan recovery rate in blood were 1.0 ppb and 104%, respectively. Finally, furan was detected in 21 individuals (13 males, 8 females) with levels ranging up to 17.86 ppb (ng furan/g food).
Keywords
furan; human blood; solid phase microextraction (SPME); gas chromatography/mass spectrometry (GC/MS);
Citations & Related Records

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1 NTP. The 9th Report on Carcinogenesis, Furan CAS No. 110-00-9 (Carcinogenicity). National Toxicology Program, The National Institute of Environmental Health Dervices, Durham NC, USA (1993)
2 Federica B, Maria C, Alessandro M, Marilena M. Development and validation of a solid phase micro-extraction-gas chromatographymass spectrometry method for the determination of furan in babyfood. J. Chromatogr. A 1102: 268-272 (2006)   DOI   PUBMED   ScienceOn
3 Mark J, Pollien P, Lindinger C, Blank I, Mark T. Quantitation of furan and methylfuran formed in different precursor systems by proton transfer reaction mass spectrometry. J. Agr. Food Chem. 54:2786-2793 (2006)   DOI   ScienceOn
4 Seaman S, Becalski A. Furan precursors in food: A model study and development of a simple headspace method for detection of furan. J. AOAC Int. 88: 102-106 (2005)   PUBMED
5 Mortelmans K, Haworth S, Lawlor T, Speck W, Tainer B, Zeiger E. Salmonella mutagenicity tests: II. Results from the testing of 270 chemicals. Environ. Mol. Mutagen. 8: 1-55 (1986)
6 Wilson DM, Goldsworthy TL, Popp JA, Butterworth BE. Evaluation of genotoxicity, pathological lesions, and cell proliferation in livers of rats and mice treated with furan. Environ. Mol. Mutagen. 19:209-222 (1992)   DOI   ScienceOn
7 IARC. IARC Monographs on the evalution of carcinogenic risks to humans. Vol. 63, p. 509. In: Dry Cleaning, Some Chlorinated Solvents and Other Industrial Chemicals. International Agency for Research on Cancer, Lyon, France (1995)
8 Health Canada. Furan in food. Available from: http://www.hcsc. gc.ca/fnan/securit/ chem-chim/food-aliment/furan/index eng.php. Accessed June 11, 2007
9 Harris DC. Exploring Chemical Analysis. 3rd ed. W.H. Freeman and Company, New York, NY, USA. pp. 91-98 (2001)
10 Altaki MS, Santos FJ, Galceran MT. Analysis of furan in foods by headspace solid-phase microextraction-gas chromatography-ion trap mass spectrometry. J. Chromatogr. A 1146: 103-109 (2007)   DOI   PUBMED   ScienceOn
11 Crews C, Castle L. A review of the occurrence, formation, and analysis of furan in heat-processed foods. Trends Food Sci. Tech. 18: 365-372 (2007)   DOI   ScienceOn
12 Chen LJ, Hecht SS, Peterson LA. Identification of cis-2-butene-1,4-dial as a microsomal metabolite of furan. Chem. Res. Toxicol. 8:903-906 (1995)   DOI   ScienceOn
13 Burka LT, Washburn KD, Irwin RD. Disposition of [14C] furan in the male F344 rat. J. Toxicol. Env. Health 34: 245-257 (1991)   DOI   PUBMED   ScienceOn
14 Peterson LA, Naruko KC, Predercki DP. A Reactive metabolite of furan, cis-2-butene-1,4-dial, is mutagenic in the Ames assay. Chem. Res. Toxicol. 13: 531-534 (2000)   DOI   ScienceOn
15 Goldmann T, Perisset A, Scanlan F, Stadler RH. Rapid determination of furan in heated foodstuffs by isotope dilution solid phase micro-extraction-gas chromatography-mass spectrometry (SPME-GC-MS). Analyst 130: 878-883 (2005)   DOI   ScienceOn
16 Kedderis GL, Carfagna MA, Held SD, Batra R, Murphy JE, Gargas MI. Kinetic analysis of furan biotransformation by F-344 rats in vivo and in vitro. Toxicol. Appl. Pharm. 123: 274-282 (1993)   DOI   ScienceOn
17 U.S. Food and Drug Administration. Furan in food, thermal treatment; Request for data and information. Available from: http://www.cfsan.fda.gov/~lrd/fr040510. html. Accessed Nov. 19, 2007.
18 Hasnip S, Crews C, Castle L. Some factors affecting the formation of furan in heated foods. Food Addit. Contam. 23: 219-227 (2006)   DOI   ScienceOn
19 Byrns MC, Vu CC, Peterson LA. The formation of substitute 1,$N^6$-etheno-2'-deoxyadenosine and 1,$N^2$-etheno-2'-deoxyguanosine adducts by cis-2-butene-1,4-dail, a reactive metabolite of furan. Chem. Res. Toxicol. 17: 1607-1613 (2004)   DOI   ScienceOn
20 Ho IP, Yoo SJ, Tefera S. Determination of furan in coffee using automated solid-phase microextraction and gas chromatography/mass spectrometry. J. AOAC Int. 88: 574-576 (2005)   PUBMED
21 U.S. Food and Drug Administration. Exploratory Data on Furan in Food data. Available from: http://www.cfsan.fda.gov/ ~dam/furandat.html. Accessed June 11, 2007
22 U.S. Food and Drug Administration. Determination of furan in foods data. Available from: http://www.cfsan.fda.gov/ ~dms/furan.html. Accessed June 11, 2007
23 Fransson-Steen R, Goldsworthy TL, Kedderis GL, Maronpot RR. Furan-induced liver cell proliferation and apoptosis in female B6C3F1 mice. Toxicology 118: 195-204 (1997)   DOI   ScienceOn
24 European Food Safety Authority. Contaminants in the food chain on provisional findings on furan in food. European Food Safety Authority Journal 137:1. Available from: http://www.efsa.eu.int/science/contam/contam_documents/760_en. html. Accessed June 11, 2007
25 Fan X, Mastovska K. Effectiveness of ionizing radiation in reducing furan and acrylamide levels in foods. J. Agr. Food Chem. 54: 8266-8270 (2006)   DOI   ScienceOn
26 Jun HJ, Lee KG, Lee YK, Woo GJ, Park YS, Lee SJ. Correlation of urinary furan with plasma $\gamma$-glutamyltranspeptidase levels in healthy men and women. Food Chem. Toxicol. 46: 1753-1759 (2008)   DOI   ScienceOn
27 Becalski A, Forsyth D, Casey V, Lau BP-Y, Pepper K, Seaman S. Development and validation of a headspace method for determination of furan in food. Food Addit. Contam. 22: 535-540 (2005)   DOI   ScienceOn
28 Senyuva HZ, Gokmen V. Analysis of furan in foods. Is headspace sampling a fit-for-purpose technique? Food Addit. Contam. 22:1198-1202 (2005)   DOI   ScienceOn
29 Maga JA. Furan in foods. CRC Cr. Rev. Food Sci. 11: 355-400 (1979)   DOI   ScienceOn
30 Byrns MC, Predecki DP, Peterson LA. Characterization of nucleoside adducts of cis-2-butene-1,4-dial, a reactive metabolite of furan. Chem. Res. Toxicol. 15: 373-379 (2002)   DOI   ScienceOn
31 Stich HF, Rosin MP, Wu CH, Powrie WD. Clastogenicity of furans found in food. Cancer Lett. 13: 89-95 (1981)   DOI   ScienceOn
32 McGregor DB, Brown A, Cattanach P, Edwards I, McBride D, Riach C, Caspary WJ. Responses of the 1517y $tk^+/ tk^-$ mouse lymphoma cell forward mutation assay: III. 72 Coded chemicals. Environ. Mol. Mutagen. 12: 85-154 (1988)   DOI   PUBMED   ScienceOn