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Study on Optimization of Cytoplasm Conditions for In Vitro Micronucleus Test Using Chinese Hamster Lung Cells (Chinese Hamster Lung Cell을 이용한 in vitro 소핵시험의 세포질 최적화 연구)

  • Paik, Min Kyoung;Kim, Areumnuri;Shin, Hye Rim;Chon, Kyongmi;Park, Kyung-Hun;Ryu, Ji-Hyeok;Moon, Byeong Chul
    • Korean Journal of Environmental Agriculture
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    • v.37 no.3
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    • pp.229-234
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    • 2018
  • BACKGROUND: in vitro micronucleus test (vitMNT) is one of the promising alternative testing methods in genotoxicity test and was adopted as OECD test guideline for chemical registration. This study was conducted to optimize the cytoplasm conditions in vitMNT using Chinese hamster lung (CHL) cell. METHODS AND RESULTS: In this study cytokinesis-block micronucleus test was conducted. Mitomycin C and colchicine were used as positive control chemicals and were treated for three hours (short time) or twenty-four hours (long time). Giemsa solution was used for cell staining. For optimization of vitMNT, the final fixative was prepared as five concentrations (0%, 1%, 3%, 5%, and 25%) of acetic acid in methanol, and treatment times of the final fixative were varied under four conditions (immediately, one hour, four hours, and one day). CONCLUSION: Acetic acid at 1% in methanol as the final fixative was most adequate to preserve the cytoplasm around the nucleus in the interphase cells. Also, fixative treatment time of cell suspension for one to four hours may minimize the cell rupture. These results can be helpful for getting an accurate result promptly due to clear visual distinction to score micronucleus in vitMNT using giemsa solution.

Bitcoin(Gold)'s Hedge·Safe-Haven·Equity·Taxation (비트코인(금)의 헷지·안전처·공평성·세제 소고)

  • Hwang, Y.
    • The Journal of Society for e-Business Studies
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    • v.23 no.3
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    • pp.13-32
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    • 2018
  • Btcoin has made a big progress through anonymity, decentralized authority, sharing economy, multi-ledger book-keeping, block-technology and the convenient financial vehicle. Bitcoin has the characteristics of mining and supply by decentralized suppliers, limited supply quantity and the partial money-like function as well as gold. The paper studies the hedge and safe-haven of Bitcoin and gold on daily frequency data over the period of July 20, 2010-Dec. 27, 2017 employing Asymmetric Vector GARCH. It finds that gold has the hedge and safe-haven against inflation and capital markets while Bitcoin has the weak hedge and the weak safe-haven. It shows insignificant effects of inflations of US and Korea on the volatilities of Bitcoin and gold. It also suggests the necessity of clearing of vagueness behind the anonymity for fair and transparent trade through the law application in the absence or fault in law (Lucken im Recht). following the spirit of the living constitution (lebendige gutes Recht oder Vorschrift). The relevant institutions are hoped to be given some of obligations such as registration, minimum required capital. report, disclosure, explanation, compliance and governance with autonomous corresponding rights. The study also suggests the reestablishment of the relevant financial law and taxation law. The hedge would not be successfully accomplished without the vigilant cautions of investors.

Anatomical Direct Reduction of Bony Mallet Finger Using Modified-Intrafocal Pinning Technique (변형-내초점 핀 고정술을 이용한 골성 망치 수지의 해부학적 직접 정복)

  • Kang, Sang-Woo;Park, Ji-Kang;Jung, Ho-Seung;Cha, Jung-Kwon;Kim, Kook-Jong
    • Archives of Hand and Microsurgery
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    • v.23 no.4
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    • pp.248-253
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    • 2018
  • Purpose: The purpose of this study was to evaluate the clinical results of anatomic reduction of bony mallet finger using modified-intrafocal pinning technique. Methods: From March 2014 to October 2017, 18 patients with bony mallet finger were treated with modified-intrafocal pinning technique. Kirschner-wire was used to directly reduction the bony fragment, and extension block pinning and distal interphalangeal joint fixation were additionally performed to minimize the loss of reduction. Postoperative pain, range of motion, and radiological evaluation were performed. Duration of bone healing, functional recovery and complication rate were evaluated and Crawford's criteria was used to determine functional outcome after surgery. Results: Bone union was achieved in all cases after a postoperative mean of 6 weeks (5-7 weeks). An average of $2.8^{\circ}$ ($0^{\circ}-10^{\circ}$) extension loss occurred in all patients. All patients showed satisfactory joint congruency and reformation of the joint surface, the mean flexion angle of the distal interphalangeal joint at the final follow-up was $72.2^{\circ}$ ($70^{\circ}-75^{\circ}$). According to Crawford's classification, 12 patients (66.7%) were excellent and 6 patients (33.3%) were good. Conclusion: Modified-intrafocal pinning technique is a method of obtaining anatomical bone healing by directly reduction and fixation of the bony fragment. Combined with other conventional percutaneous pinning procedures, it is expected that good results can be obtained if applied to appropriate indications.

The Poet Kim Shi-Jong living in both Joseon and Japan: the Meaning of 'Zainichi' Expressed in Epic Poem Niigata (조선과 일본에 사는 시인 김시종 - 장편시집 『니이가타』에 표현된 '재일'의 의미)

  • Kim, Gae-Ja
    • Cross-Cultural Studies
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    • v.45
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    • pp.7-32
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    • 2016
  • This article considers the meaning of 'Zainichi (在日)' expressed in epic poem Niigata (1970) written by Korean-Japanese poet Kim Shi Jong. Kim sets two points in his creation of poetry. One is summer of liberation released from Japanese colonial domination in 1945. The other is Japan where he lives as a Korean-Japanese. These two points have made him think about the meaning of living in post-colonial era and the national division of Korea, his home country. His thought like this is well expressed in his epic Niigata. Niigata was written in 1959 when the ship returning to North Korea departed from Niigata of Japan. However, Kim couldn't return to his home country at that time. He stowed away from South Korea to Japan in 1949. He participated in antigovernment activities occurred in Jeju Island to block the national division between the south and the north after the liberation in 1945, the so-called 4.3 incident. Besides, he was having conflict with the organization of North Korea at that time because it required a doctrinaire belief and creation in Korean. Kim was writing poems in Japanese and pursued the life of existence as a Korean-Japanese. Therefore, he decided to remain in Japan instead of returning to North Korea. Of course, he could not return to South Korea because he was a refugee. Kim imagined in Niigata, the place located in an extension of the 38th parallel and the spot of national division. He could not cross the division line when he was in his home country, but he could do it in Niigata through imagination. The life as a Korean-Japanese makes it possible. 'Zainichi', which means living in Japan, has been recognized as a worse situation compared to living in Korea. However, Kim changed his way of thinking. Zainichi can embrace South Korea, North Korea, and Japan. This is the very reason why he lives there as a Korean-Japanese. His thought like this is well expressed by symbolic representations and metamorphose as well as the imagination of spatial extension.

A Review of the Legal Nature that Users of the Virtual Currency Exchange Obtain and the Compensation Responsibility for the Damages Caused By Internet Problems or Network Errors (가상통화거래소 이용자가 가지는 법적 성격과 전산장애로 인한 손해배상 책임 연구)

  • Choi, JangWon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.11
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    • pp.287-294
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    • 2018
  • This thesis covers legal aspects of the crypto-currency exchange and the legal rights of crypto-currencies holders. Unlike financial markets in which central authorities or intermediaries determine the validity of transactions and manage records, crypto-currency markets utilize a decentralization system based on block chain technology. Such distinct characteristics distinguish crypto-currency from currency, notes, or financial instruments. Therefore, we need to check closely the legal principles that are applicable to crypto-currency. Crypto-currency users possess rights indirectly through the crypto-currency exchange. However, we should look at whether crypto-currency can be an object of ownership. This research found that legal protection for crypto-currency exchanges are limited. Domestic laws have many shortcomings to protect users' rights. This study found that users who incurred damages due to internet computation errors at exchanges require a protective system like stock markets. Therefore, studies on the legal controls and system regulations are required to protect users' rights. Also, crypto-currency information exchanges keep inside and protections for users' private information need to be further examined.

The Effect of Stellate Ganglion Block on Breast Cancer-Related Infectious Lymphedema (유방암 감염성 림프부종 환자에서 성상신경절 블록이 미치는 영향)

  • Lee, Youn Young;Park, Hahck Soo;Lee, Yeon Sil;Yoo, Seung Hee;Lee, Heeseung;Kim, Won Joong
    • Journal of Hospice and Palliative Care
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    • v.21 no.4
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    • pp.158-162
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    • 2018
  • Breast cancer related lymphedema (BCRL) is one of the most intractable complications after surgery. Patients suffer from physical impairment, as well as psychological depression. Moreover, a recent study revealed that cellulitis significantly increased the risk of BCRL, and cellulitis has been suggested as a risk factor of BCRL development. We describe a patient treated with stellate ganglion blocks (SGBs) without steroid for relief of symptoms and reduction of the arm circumference of breast cancer-related infectious lymphedema in a month. We measured the arm circumference at four locations; 10 cm and 5 cm above and below the elbow crease, numeric rating scale (NRS) score, lymphedema and breast cancer questionnaire (LBCQ) score on every visit to the pain clinic. A serial decrease of the arm circumference and pain score were observed after second injection. In the middle of the process, cellulitis recurred, we performed successive SGBs to treat infectious lymphedema. The patient was satisfied with the relieved pain and swelling, especially with improved shoulder range of motion as it contributes to better quality of life. This case describes the effects of SGB for infectious BCRL patients. SGB could be an alternative or ancillary treatment for infectious BCRL patients.

Efficient Humidity Color Sensor Based on a Photonic Crystal with a Metal-Organic Framework (금속-유기 구조체를 이용한 포토닉 크리스탈 기반의 효율적인 습도 컬러 센서)

  • Kim, Jun Yong;Lee, Sung Hak;Do, Yun Seon
    • Korean Journal of Optics and Photonics
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    • v.29 no.6
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    • pp.268-274
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    • 2018
  • In this study we suggest a humidity-sensitive color sensor using a one-dimensional photonic crystal and Hong Kong University of Science and Technology-1 (HKUST-1), which is a metal-organic framework (MOF) substance. One-dimensional photonic crystals have a photonic band gap, due to a periodic refractive-index change, and block and reflect light components in a specific wavelength band. The refractive index of HKUST-1 differs in dry and humid environments. Herein we designed a sensor using the presence of the photonic band gap, with FDTD simulation. As a result of optical analysis, the color conversion of the reflected light was superior to the color conversion of the transmitted light. When the center wavelength of the photonic band gap was 550 nm, the maximum peak value of the wet environment increased by a factor of about 9.5 compared to the dry environment, and the color conversion from achromatic to green was excellent as a sensor. The results of this study suggest the application of MOF materials to moisture sensors, and the nanostructure design of MOF materials will expand the applications to industrial devices.

12-bit SAR A/D Converter with 6MSB sharing (상위 6비트를 공유하는 12 비트 SAR A/D 변환기)

  • Lee, Ho-Yong;Yoon, Kwang-Sub
    • Journal of IKEEE
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    • v.22 no.4
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    • pp.1012-1018
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    • 2018
  • In this paper, CMOS SAR (Successive Approximation Register) A/D converter with 1.8V supply voltage is designed for IoT sensor processing. This paper proposes design of a 12-bit SAR A/D converter with two A / D converters in parallel to improve the sampling rate. A/D converter1 of the two A/D converters determines all the 12-bit bits, and another A/D converter2 uses the upper six bits of the other A/D converters to minimize power consumption and switching energy. Since the second A/D converter2 does not determine the upper 6 bits, the control circuits and SAR Logic are not needed and the area is minimized. In addition, the switching energy increases as the large capacitor capacity and the large voltage change in the C-DAC, and the second A/D converter does not determine the upper 6 bits, thereby reducing the switching energy. It is also possible to reduce the process variation in the C-DAC by proposed structure by the split capacitor capacity in the C-DAC equals the unit capacitor capacity. The proposed SAR A/D converter was designed using 0.18um CMOS process, and the supply voltage of 1.8V, the conversion speed of 10MS/s, and the Effective Number of Bit (ENOB) of 10.2 bits were measured. The area of core block is $600{\times}900um^2$, the total power consumption is $79.58{\mu}W$, and the FOM (Figure of Merit) is 6.716fJ / step.

Behaviour of Ground Anchor According to Period Characteristic of Seismic Load Using Numerical Analysis (수치해석을 통한 지진하중의 주기특성에 따른 그라운드 앵커의 거동)

  • Oh, Dong-Wook;Jung, Hyuk-Sang;Yoon, Hwan-Hee;Lee, Yong-Joo
    • Tunnel and Underground Space
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    • v.28 no.6
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    • pp.635-650
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    • 2018
  • Many people have been recognized that the Korean Peninsula is no longer safe area from the earthquake by the recent earthquakes occurred in the country. The earthquakes that occurred at Pohang and Gyeongju appeared differently from them considered in the seismic design and researches on the seismic design method have been also conducted by many researchers. Studies on seismic loads are mainly focused on existing superstructures, and research involving them has been actively carried out in reality. However, paper regarding structural stability of reinforcement from seismic load such as soil-nails, rock-bolts, ground anchors which were constructed to ensure stability of serviced structure have been published rarely. In this study, ground anchor been effected by static load and seismic load which is settled in the weathered rock is analyzed. Results for static load are obtained from field test and seismic load is from numerical analysis. In this study, the behavioral characteristics of the ground anchor were analyzed by numerical analysis in case of seismic loading based on the result of the in-situ tensile test of the ground anchor settled weathered rock. As a result, settlement of concrete block due to application of tension force for ground anchor occurred as well as following loss of axial force for ground anchor. Also, as bond length and period of seismic load are longer, increasement of displacement is greater.

International Research Status on Spent Nuclear Fuel Structural Integrity Tests Considering Vibration and Shock Loads Under Normal Conditions of Transport (정상운반조건의 진동 및 충격하중을 고려한 사용후핵연료의 구조적 건전성 시험평가 해외연구현황)

  • Lim, JaeHoon;Cho, Sang Soon;Choi, Woo-seok
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.17 no.2
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    • pp.167-181
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    • 2019
  • Currently, the development of evaluation technology for vibration and shock load characteristics and spent nuclear fuel structural integrity under normal conditions of transport is being conducted in the Republic of Korea. This is the first such research conducted in the Republic of Korea and, thus, previous international studies need to be investigated and will be referred to in the ongoing project. Before 2000, several studies related to measurement of vibration and shock loads on spent nuclear fuel were conducted in the US. US national research institutes conducted uniaxial fuel assembly shaker tests, concrete block tests, and multi-axis fuel assembly tests between 2009 and 2016. In 2017, multi-modal transportation tests including road, sea, and rail transport were also performed by research institutes from the US, Spain and the Republic of Korea. Therefore, test preparation procedures, acceleration and strain measurement results, and finite-element and multi-body dynamics analysis were investigated. Based on the measured strain data, the preliminary conclusion was obtained that the measured strain was too small to cause damage to spent nuclear fuel rods. However, this conclusion is a preliminary conclusion that only reviews part of the results; a detailed review is being conducted in the US. The investigation of international studies on spent nuclear fuel structural integrity tests considering vibration and shock loads under normal conditions of transport in the US will be useful data for the project being conducted in the Republic of Korea.