• Journal of Korean Academy of Oral Health
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• v.42 no.4
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• pp.210-215
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• 2018

Objectives: The main objectives of this study were to verify the antibacterial activity of two essential oils, lavender and peppermint, against dental caries and to review their synergistic effect when used in combination. Our results provide basic data for the evaluation of the use of these two substances towards the prevention and cure of dental caries. Methods: The sample solutions of lavender and peppermint oils were prepared in three different concentrations (30%, 50%, and 70% (v/v)) by diluting them with third-distilled water and Tween 20. Streptococcus mutans was selected as the bacterial species for testing. The disk diffusion method was used to measure the antibacterial activity of the sample solutions. For generating growth curves and measuring the number of clusters of the bacterial, the liquid medium-dilution method was used; the absorbance of the medium was measured at 600 nm after 3, 6, 12 and 24 hours. Results: When the antibacterial activity of the oils was tested via the disk diffusion method, the activity improved with increasing concentrations of all the sample solutions of peppermint, lavender, and the blend, but there was no significant difference between them with respect to the type of oil. In the growth curves of S. mutans, growth inhibition was observed after 12 hours. The inhibitory effect of 30% lavender oil on growth was 64.9% and 80.1% after 12 and 24 hours of treatment, respectively whereas that of peppermint oil was 71.3% and 80.1% after 12 and 24 hours of treatment, respectively. The inhibitory effect of the blended oil was 71.9% and 81.0% after 12 and 24 hours of treatment, respectively. Conclusions: Further research is still required in order to determine the efficacy of lavender and peppermint oils, as well as other essential oils, for wider use in preventing dental caries.

• Korean Journal of Veterinary Research
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• v.49 no.1
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• pp.85-89
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• 2009

This study examined the therapeutic effects of aromatherapy for the treatment of otitis externa in dogs. Eleven dogs with otitis externa were examined. The control group (5 dogs) was treated with susceptible antibiotics, and the experimental group (6 dogs) was treated with aroma-oil applied topically to the ear canal. The aroma-oil contained 10 ml sweet almond oil, 0.3 ml bergamot oil, 0.2 ml lavender oil, 0.1 ml tea tree oil and 0.1 ml roman chamomile oil. The blended aroma-oil (0.1 ml) was applied to the ear canal twice daily for 2 weeks. The authors examined the changes in the clinical signs, bacterial count in discharges, total WBC count and neutrophil/lymphocyte ratios in the two groups. The bacterial cell counts in the experimental group were significantly lower at one (p < 0.01) and two weeks (p < 0.05) after treatment than the control group. These results suggest that aromatherapy is an effective and practical treatment for otitis externa in dogs.

• International Journal of Internet, Broadcasting and Communication
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• v.14 no.3
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• pp.171-181
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• 2022

Purpose: In this paper, we show our blending ratio of 10 types of Essential Oils that survives beneficial bacteria and kills harmful bacteria in the scalp, and we investigate the possibility of application of our blending ratio to the development of anti-dandruff products and the possibility of being used as a raw material for clinical beauty and customized cosmetics. Methods: The scalp microorganisms used in our study were M. furfur, S. epidermidis, E. coli, and P. nitroreducens. There are a total of 10 Essential Oils such as True Lavender, Lime, Roman chamomile, Rosemary camphor, Cedarwood, Geranium, Clove, Tea tree, Palmalosa, and Peppermint. The antibacterial test of the blended Essential Oil was carried out according to the test method of the standardized evaluation methodology of "Food and Food Additives Code". Since M. furfur is related to the growth of sebum in the scalp, in this study we used the fnLNB and the fnLNA with 20 ㎖ of whole fat cow milk added. Results: The blending ratio of EO, which inhibits dandruff-causing bacteria such as M. furfur, S. epidermidis, E. coli, and does not inhibit P. nitroreducens showing dominant growth in a healthy scalp, was B8(Clove 0.2%, Roman chamomile 0.5%, Tea tree 0.3%), B9(Geranium 0.1%, Palmarosa 0.1%, Roman chamomile 0.5%, Tea tree 0.3%), B10(Clove 0.1%, Geranium 0.1%, Palmarosa 0.1%, Roman chamomile 0.5%, Tea tree 0.2%). Conclusion: It is thought that the blending ratio of BEO obtained as a result of this study can provide a basis for use as an alternative to antibiotics in developing anti-dandruff drugs and emerge as a new alternative to solve scalp microbial imbalance. In order for EO to be used as a useful raw material for anti-dandruff preparation, researches on 1) Standardization (the effects of products differ according to the types, regions, climate, extraction methods, etc.), 2) Antimicrobial effects, 3) Safety, etc., must be established.

• Journal of the Korean Applied Science and Technology
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• v.38 no.6
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• pp.1370-1382
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• 2021

Previous studies have been conducted on domestic materials as a single extract. Research on complex mixtures for maximizing plant characteristics by individual extraction and potential interference with effects is insufficient. Therefore, this study confirmed the GC-MSD according to the extraction of essential oils for Agastache rugosa O. Kuntze(AR), Pinus densiflora Sieb. et Zuccarini(PD), Curcuma longa, Curcuma domestica(CC), Zingiber officinale Roscoe(ZR), Foeniculum vulgare Miller(FV), and Citrus medica L. var. sarcodactylis Swingle(CS). The cytotoxicity, antioxidant, and anti-inflammatory properties of the blending oil were confirmed to confirm its potential as a cosmetic material. As a result of analyzing GC-MSD aroma components, the main components were estragole of AR, à-Pinene for PD, Zingiberene for CC and ZR, Anethole from FV, and D-Limonene for CS. At a concentration of 100 uL/mL with no confirmed cytotoxicity, NO production was inhibited by 70.62%, DPPH radical scavenging activity was 64.03%, and ABTS radical scavenging activity was 89.55%. Through this, blended essential oil suggests the possibility of useful application as a raw material with antioxidant and anti-inflammatory effects in the cosmetic and food industries.

• Journal of Korean Biological Nursing Science
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• v.12 no.1
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• pp.16-23
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• 2010

Purpose: The purpose of this study was to examine the effects of aromatherapy on blood pressure, pulse, fatigue and sleep for patients with allergic rhinitis admitted at a hospital specialized in an oriental medicine. Methods: This research was a single-group pretest-posttest design. Data were collected at admission and discharge from May to December, 2009. The patients selected were received an aroma inhalation therapy via nebulizer with a 2% diluted oil mixture of eucalyptus and peppermint with 10 cc of saline solution in the ratio of 1:1 ones a day for 15 min in the period of 2 weeks. The data were analyzed for the descriptive statistics and paired t-test. Results: There was a statistically significant improvement on physical and psychological symptoms of fatigue and sleep with a increase in systolic blood pressure. Conclusion: Eucalyptus and peppermint blended oil aromatherapy as a sleep induction modality is an effective nursing and therapeutic intervention in conjunction with acupuncture and herbal medicine for symptoms of nasal congestion. However, further well designed research is required in evaluating the effectiveness of the types of clinically available essential oil.

• Journal of Animal Science and Technology
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• v.45 no.5
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• pp.777-786
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• 2003

An experiment was conducted to investigate the effects of supplementary Blended essential oil(CRINA$^{\circledR}$) on the performance, nutrient availability, fatty acid composition of leg muscle, small intestinal microflora and blood parameters in broiler chickens. One thousand unsexed day-old broiler chickens were assigned to five treatments : control(T1), 5ppm avilamycin(starter diet) & 5ppm flavomycin(grower diet) T2, 5ppm avilamycin(starter diet) & 50ppm CRINA$^{\circledR}$(grower diet) T3, 50ppm CRINA$^{\circledR}$(starter & grower diet) T4, 50ppm CRINA$^{\circledR}$+ 500ppm lactic acid$^{\circledR}$ (starter & grower diet) T5. Each treatment had four replications of 50 birds each. Growth performance was significantly improved by dietary supplements(T2-T5). There were no significant differences among treatment T2, T3, T4 and T5. Feed intake was not significantly different among treatments. Dietary supplementation of CRINA$^{\circledR}$(T3, T4, T5) resulted in significant(p〈0.05) improvement in feed/gain(F/G) during finishing period (4-5weeks). The birds fed CRINA$^{\circledR}$ supplemented diet(T4) showed significantly(p〈0.05) higher availability of crude fat, methionine and methionine + cystine than those fed antibiotics supplemented diet(T2). Mortality was not significantly affected by treatments. The colony forming unit(CFU) of E.coli in small intestinal content was significantly lower in antibiotics & CRINA$^{\circledR}$(T3) compared to CRINA$^{\circledR}$ treatment(T4)(P〈0.05). CFU of Cl. perfringens was low in CRINA$^{\circledR}$(T4) but not different significantly with other treatments. Serum triglyceride level of birds fed CRINA$^{\circledR}$ + lactic acid diet(T5) was significantly lower(p〈0.05) than those fed antibiotics supplemented diet(T2). Cholesterol level of the birds fed antibiotics(T2) or CRINA$^{\circledR}$ + lactic acid supplemented diet(T5) was significantly higher(p〈0.05) than other treatments. HDL level of birds fed control diet was significantly lower(p〈0.05) than that of others. The levels of serum IgG were not significantly different among treatments. Major fatty acids composition of leg muscle fat was significantly influenced by treatments. Control group showed significantly higher palmitic acid(C$_{16:0}$) and steraric acid(C$_{18:0}$) content than other treatments(p〈0.05). Content of oleic acid(C$_{18:1}$), however, was significantly lower in the control than others treatments. Content of linolenic acid(C$_{18:3}$) was significantly higher in CRINA$^{\circledR}$+ lactic acid(T5) than antibiotics & CRINA$^{\circledR}$(T3) treatments. Total saturated fatty acids content was higher and total unsaturated fatty acids were lower in the leg muscle fat of the control than that of other treatments. It is concluded that CRINA$^{\circledR}$ supplementation improved growth rate and F/G ratio in broilers. The combination of CRINA$^{\circledR}$ with either antibiotics or lactic acid did not show any additive or synergistic effects in broiler chickens .

• Journal of Pharmacopuncture
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• v.13 no.1
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• pp.53-61
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• 2010

Purpose : To investigate the effects of the lavender, lemon and eucalyptus oil mixture on the atopy dermatitis skin lesions induced on NC/Nga Mice by dinitrochlorobenzene (DNCB). Material and Method : For this purpose, we fabricated the oil mixture blending three essential oils (lavender, lemon, eucalyptus : ELL) with one carrier oil (jojoba) and apply it on the atopic dermatitis skin lesions of NC/Nga Mice. Atopic dermatitis in NC/Nga Mice was induced by DNCB treatment on the dorsal skin of mice for 8 weeks. The mixture of ratio of each essential oil drop was 1 (eucalyptus) : 2 (lemon) : 2 (lavender) and this mixture was blended with jojoba oil 50ml (0.025%). The ELL-ointment was supplied for 8 weeks. We evaluated the effects of ELL on cell viability of mouse lung fibroblast, clinical skin features and severity, the level of serum Immunoglobulin (Ig) E & Ig G1, Interleukin (IL)-4, IL-13 and Interferon (IFN)-$\gamma$. Results : ELL showed safety on the cell viability of mouse lung fibroblast compared with control group. The cell viability was measured by SRB method. The effects of ELL on clinical skin features and severity in DNCB-induced dermatitis model of NC/Nga mice was significant compared with control group. EEL also showed significant effects on clinical symptom score compared with control group. Serum IgE & IgG1 level and development of atopy dermatitis skin lesions were evaluated. Serum IgE & IgG1 production was significantly down-regulated in EEL group compared with control group. ELL also down-regulated the levels of IL-4 and IL-13, and up-regulated the level of IFN-$\gamma$ compared with control group significantly. Conclusion : ELL was effective on atopy dermatitis by modulating Th2 related factors.

• Journal of Korean Academy of Nursing
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• v.35 no.1
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• pp.186-194
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• 2005

Purpose: The purpose of this study was to investigate the effect of aromatherapy on pain, depression, and feelings of satisfaction in life of arthritis patients. Method: This study used a quasi-experimental design with a non-equivalent control group, pre-and post-test. The sample consisted of 40 patients, enrolled in the Rheumatics Center, Kangnam St. Mary's Hospital, South Korea. The essential oils used were lavender, marjoram, eucalyptus, rosemary, and peppermint blended in proportions of 2:1:2:1:1. They were mixed with a carrier oil composed of almond (45%), apricot(45%), and jojoba oil(10%) and they were diluted to 1.5% after blending. The data were analyzed using an 2-test, Fisher's exact test, t-test and paired t-test. Result: Aromatherapy significantly decreased both the pain score and the depression score of the experimental group compared with the control group. However, aromatherapy didn't increase the feeling of satisfaction in life of the experimental group compared with the control group. Conclusion: The result of this study clearly shows that aromatherapy has major effects on decreasing pain and depression levels. Based on our experiment's findings, we suggest that aromatherapy can be a useful nursing intervention for arthritis patients.

• Journal of Korean Biological Nursing Science
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• v.6 no.2
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• pp.5-17
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• 2004

Purpose: The purpose of this study is to determine effects of aromatherapy on the activity and balance of autonomic nervous system and on physical resistance to stress. Methods: This research is a primitive experimental design which attempts to analyze aromatherapy EKG monitoring on real-time basis. Lavender & Chamomile, each 30 drops, were blended with 100ml Sweet Almond oil. Subjects was laid relaxed, and had insides of his wrists and ankles attached with electrodes for EKG. Right after applied with essential oil onto his philtrum and parotid, started on EKG monitoring. Results: The activities of parasympathetic and sympathetic nerve were changed as time elapsed, with significant differences(p=.000). In multiple comparison, the value was significantly different(p<.05).The balance of autonomic nerve was changed, becoming close to the normal level in accord with applicable international standards(1.5)(p=.011). In multiple comparison, the value showed a significant difference(p<.05). Physical resistance to stress increased with the passage of time, but not statistically significant. Conclusions: This suggests that aromatherapy can be used as a nursing intervention which aims at alleviating symptoms related to the imbalance of autonomic nerve system such as headache, hot flashes, irregular heartbeat, nervousness, depression and anxiety.

• Clean Technology
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• v.22 no.2
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• pp.96-105
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• 2016

VOCs emissions from industries cause the air pollution and odor. In the industrial facilities, the existing odor treatment techniques have limits and problems. In this study, the optimum essential oil and metal oxide selected by screening test. lavender oil, cypress oil and TiO₂ were determined by deodorant materials and those were blended by 5%, 45%, 10%, respectively. In addition, the result of batch type experiments depending on the dilution rate, injection, rate, temperature showed that the optimum condition of deodorant is 6 mL of injection rate, and 200 times of dilution rate and the removal efficiency increased in proportion with temperature. In addition, the activation energy was calculated from the rate equation, which appeared in the 3-4 times lower than conventional deodorants.