Journal of Korean Academy of Oral Health

The Korean Academy of Preventive Dentistry and Oral Health (대한예방치과·구강보건학회)
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Anti-bacterial effects of lavender and peppermint oils on Streptococcus mutans

Streptococcus mutans 대한 Lavender와 Peppermint Oil의 항균효과

  • Park, Chung Mu (Department of Clinical Laboratory Science, Dong-Eui University) ;
  • Yoon, Hyun Seo (Department of Dental Hygiene, Dong-Eui University)
  • 박충무 (동의대학교 임상병리학과) ;
  • 윤현서 (동의대학교 치위생학과)
  • Received : 2018.10.02
  • Accepted : 2018.11.22
  • Published : 2018.12.30
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Abstract

Objectives: The main objectives of this study were to verify the antibacterial activity of two essential oils, lavender and peppermint, against dental caries and to review their synergistic effect when used in combination. Our results provide basic data for the evaluation of the use of these two substances towards the prevention and cure of dental caries. Methods: The sample solutions of lavender and peppermint oils were prepared in three different concentrations (30%, 50%, and 70% (v/v)) by diluting them with third-distilled water and Tween 20. Streptococcus mutans was selected as the bacterial species for testing. The disk diffusion method was used to measure the antibacterial activity of the sample solutions. For generating growth curves and measuring the number of clusters of the bacterial, the liquid medium-dilution method was used; the absorbance of the medium was measured at 600 nm after 3, 6, 12 and 24 hours. Results: When the antibacterial activity of the oils was tested via the disk diffusion method, the activity improved with increasing concentrations of all the sample solutions of peppermint, lavender, and the blend, but there was no significant difference between them with respect to the type of oil. In the growth curves of S. mutans, growth inhibition was observed after 12 hours. The inhibitory effect of 30% lavender oil on growth was 64.9% and 80.1% after 12 and 24 hours of treatment, respectively whereas that of peppermint oil was 71.3% and 80.1% after 12 and 24 hours of treatment, respectively. The inhibitory effect of the blended oil was 71.9% and 81.0% after 12 and 24 hours of treatment, respectively. Conclusions: Further research is still required in order to determine the efficacy of lavender and peppermint oils, as well as other essential oils, for wider use in preventing dental caries.

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References

  1. Choi HS. Influencing factors of dental caries across the life cycle of Koreans. J Korean Soc Dent Hyg 2017;17:889-898.
  2. Kim KE, Ahn ES, Han JH. Variation in the index of dental plaque removal and practice assessment after instruction on toothbrushing. J Dent Hyg Sci 2015;15:220-225. https://doi.org/10.17135/jdhs.2015.15.2.220
  3. Jeon EY, Lee SY. Remineralization effect according to application cycle of fluoride varnish: QLF-D analysis. J Korean Soc Dent Hyg 2016;16:525-530. https://doi.org/10.13065/jksdh.2016.16.04.525
  4. Kang JM, Im SU, Jo HY, Ma JK, Kim JS, Kim KH et al. Adhesive characteristics of Mutans Streptococci on the surface of filling materials and sealant. Korean J Dent Mater 2015;42:229-238. https://doi.org/10.14815/kjdm.2015.42.3.229
  5. Korea Health Promotion Institute. The 4th Health Plan 2020: Trend Report 2017. Seoul:Korea Health Promotion Institute;2017:77.
  6. Briner WW et al. Plaque in relation to dental caries and periodontal disease. Int Dent J 1971:21:293-301.
  7. Scheie AA. Modes of action currently known chemical antiplaque agents other than chlorhexdine. J Dent Res 1989;68(spec iss):1609-1616.
  8. Lee JS, Chung KH. Antimicrobial effect of Prunus mume extracts against cariogenic bacteria. J Korean Acad Oral Health 2017;41:65-70. https://doi.org/10.11149/jkaoh.2017.41.1.65
  9. Kim SS, Won JH, Lee GE, Lee RR, Lee JH, Kang KH. Anti-bacteria effect of green tea, mulberry, and mate leaves extracts on S. mutans. Journal of Digital Convergence 2017;15:347-353.
  10. Lee HW, Lee P, Kwon, Han KI, Hann MD. Antimicrobial activity of extracts from some traditional oriental medicinal plants against dental caries bacteria. J Dent Hyg Sci 2013;13:45-52.
  11. Baek HS, Kang SK, Auh QS, Chun YH, Hong JP. Effect of antibacterial effects of myrrh, rhatany, chamomomilla against to oral microorganisms. J Oral Med Pain 2013;38:299-312. https://doi.org/10.14476/jomp.2013.38.4.299
  12. Choi HJ, Heo NS, Choi TW, Lee YG, Jeong YK, Joo WH. Antimicrobial and Anti-halitosis Effects of Alnus firma Extracts. J Life Sci 2012;22:1071-1076. https://doi.org/10.5352/JLS.2012.22.8.1071
  13. Lee YS, Kim SK, Yang TC, Kim GS, Jeon JG, Chang KW. The antibacterial and growth inhibitory effect of some essential oils against the oral micro-organisms. J Korean Acad Oral Health 2006;30:490-496.
  14. Kim EH. Anticariogenic effect of chamaecyparis obtusa essential oil on Streptococcus mutans[master's thesis]. Chonbuk:Wonkwang University;2016.[Korean].
  15. Choi JY. Antibacterial effects of clove oil against oral bacteria and its other biological activities [master's thesis]. Chungnam:Hoseo University;2018.[Korean].
  16. Kazemzadeh R, Nikjou R, Rostamnegad M, Norouzi H. Effect of lavender aromatherapy on menopause hot flushing: A crossover randomized clinical trial. J Chin Med Assoc 2016;79:489-492. https://doi.org/10.1016/j.jcma.2016.01.020
  17. Sadeghzadeh J, Vakili A, Bandegi AR, Sameni HR, Zahedi Khorasani M, Darabian M. Lavandula reduces heart injury via attenuating tumor necrosis factor-alpha and oxidative stress in a rat model of infarct-like myocardial injury. Cell J. 2017;19:84-93.
  18. Lee JY, Ko MY, Kim JS, Park CI. Inhibitory effect of lavender essential oil on formalin-induced ultrasonic vocalizations in rats. J Soc Cosmet Sci Korea 2015;5:203-208.
  19. Yamamoto J, Yamada K, Naemura A, Yamashita T, Arai R. Testing various herbs for antithrombotic effect. Nutrition 2005;21:580-587. https://doi.org/10.1016/j.nut.2004.09.016
  20. Schelz Z, Molnar J, Hohmann J. Antimicrobial and anti plasmid activities of essential oils. Fitoterapia 2006;77:279-285. https://doi.org/10.1016/j.fitote.2006.03.013
  21. Oh JY, Lee BS, Kim YC. Hair growth promotion effect and antibacterial activity against pityrosporum ovale of peppermint oil. J Invest Cosmetol 2014;10:261-269. https://doi.org/10.15810/jic.2014.10.4.002
  22. Wilson R. Aromatherapy essential oils for vibrant health and beauty. Penguin Putnam Inc. NY;2002:107-109.
  23. Kwon PS, Kim DJ, Park H. Improved antibacterial effect of blending essential oils. Korean J Clin Lab Sci 2017;49:256-262. https://doi.org/10.15324/kjcls.2017.49.3.256
  24. Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Analy 2016;6:71-79. https://doi.org/10.1016/j.jpha.2015.11.005
  25. Kim HS, Kwon HS, Kim CH, Lee SW, Kongmany S, Cho SJ. Effects of methanol extracts from diospyros malabarica stems on growth and biofilm formation of oral bacteria. J Life Sci 2018;28:110-115.
  26. Lee SE, Hwang HJ, HA JS, Jeong HS, Kim JH. Screening of medicinal plant extracts for antioxidant activity. Life Sci 2003;73:167-179. https://doi.org/10.1016/S0024-3205(03)00259-5
  27. Campos CA, Gerschenson LN, Flores SK. Development of edible films and coatings with antimicrobial activity. Food Bioproc Tech 2011;4:849-875. https://doi.org/10.1007/s11947-010-0434-1
  28. Bhagava K, Conti DS, da Rocha SRP, Zhang Y. Application of and oregano oil nanoemulsion to the control of foodborne bacteria on fresh lettuce. Food Microbiol 2015;47:69-73. https://doi.org/10.1016/j.fm.2014.11.007
  29. Kim MS, Lee KW, Park EJ. Antimicrobial activity of lavander and rosemary essential oil nanoemulsions. Korean J Food Cook Sci 2017;33:256-263. https://doi.org/10.9724/kfcs.2017.33.3.256
  30. Chaudhari LKD, Jawale BA, Sharma S, Sharma H, Mounesh Kumar CD, Kulkarni PA. Antimicrobial activity of commercially available essential oils against streptococcus mutans. J Contemp Dent Paract 2012;13:71-74. https://doi.org/10.5005/jp-journals-10024-1098
  31. Kim SY, Kim HN, Jun EJ, Kim JB, Jeong SH. The growth inhibitory effect of some vegetable oils on Streptococcus mutans and lactobacillus casei. J Korean Acad Oral Health 2016;40:24-30. https://doi.org/10.11149/jkaoh.2016.40.1.24
  32. Choi YR, Kang MK. Antibacterial effect of tea tree on streptococcus mutans. J Korean Soc Dent Hyg 2017;17:613-620.