• 한국가금학회지
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• 제41권3호
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• pp.191-196
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• 2014

가금 사육 프로그램에서 경제적으로 중요한 형질에 잠재적인 후보 유전자의 식별 및 활용은 점점 더 중요해지고 있다. Ovocalyxin-32(OCX-32) 유전자는 닭의 9번 염색체에 위치하며, 난각을 형성하는데 중요한 역할을 한다. 본 연구의 목적은 한국 재래닭의 OCX-32 유전자 내 SNP의 유전자형 결정과 산란 형질과의 연관성을 분석하기 위해 수행하였다. PCR-RFLP 방법을 통해 한국 오골계 46수, 백색 46수, 회색 43수, 흑색 46수를 포함한 총 181수의 한국 재래닭 암컷 4종의 SNP을 분석하였다. 산란 형질은 시산일령, 시산난중, 산란율, 난중의 4가지 항목을 포함하여 측정하였다. OCX-32 유전자 내 c.494A>C SNP은 오골계의 산란율과 유의적인 차이를 나타냈으며(p<0.001), 백색 재래닭에서는 난중과 유의적인 상관관계가 있었다(p<0.05). c.267T>G SNP은 오골계의 난중과 유의적인 연관성이 나타났다(p<0.05). 하지만 회색과 흑색 재래닭에서는 유의적인 상관관계가 나타나지 않았다. 본 연구 결과, 한국 재래닭의 사육 프로그램에서 산란형질을 선발하는 마커로 사용되기까지 추후 더 많은 개체군을 통한 연구가 요구되나, OCX-32 유전자 내 c.494A>C와 c.267T>G의 단일염기변이가 한국 재래닭 중 오골계와 백색 재래닭에서 산란 특성에 따른 DNA 선발 마커로서 활용할 수 있을 것으로 사료된다.

• 해양환경안전학회지
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• 제22권5호
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• pp.547-553
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• 2016

선박용 디젤엔진 배기가스에 포함된 입자상물질(PM) 가운데서 블랙카본(BC)은 극지방의 해빙을 촉진시키고 온난화를 유발하는 원인물질로서 큰 주목을 받고 있다. 본 연구에서는 향후 예상되는 PM/BC 배출 규제에 선제적으로 대응하고, 저감기술 개발 및 실증을 위한 기초연구의 일환으로 한국해양대학교 실습선 한바다호를 이용하여 실제 운항 중에 주기관의 배기관 수 포인트에서 PM을 샘플링하여 HR-TEM을 통해 그 구조와 특성을 분석하였다. 또한 배기가스 분석기를 이용하여 운전조건별 배기가스 내 유해물질의 배출 경향을 확인하였다. 분석 결과 엔진에서 배출된 PM의 구조는 구형 입자들의 촘촘하지 않은 체인형 결합으로 이루어져 있으며, 과급기로부터 멀어질수록 온도 저하에 의한 응집이 더 많이 관찰되었고 BC 특유의 graphitic 구조를 잃어가면서 점점 amorphous 구조를 띠는 경향이 나타났다. 또한 배기가스 분석을 통해 엔진 회전수가 증가할수록 배기 내 PM의 농도는 감소할 것으로 예측되었다.

• Animal Bioscience
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• 제34권8호
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• pp.1392-1402
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• 2021

Objective: The growth rate of pigs is related to differentiation and proliferation of muscle cells, which are regulated by growth factors and expression of growth-related genes. Thus, the objective of this study was to establish optimal culture conditions for Jeju black pig (JBP) muscle cells and determine the relationship of various factors involved in muscle growth with the proliferation of JBP muscle cells. Methods: Muscles were taken from the femur skeletal muscle of JBP embryos. After isolation of the muscle cells, cells were cultured in a 6-well plate under four different culture conditions to optimize culture conditions for JBP muscle cells. To analyze proliferation rate of JBP muscle cells, these muscle cells were seeded into 6-well plates at a density of 1.5×10⁵ cells per well and cultured for 3 days. Western blot and quantitative real-time polymerase chain reaction were applied to verify the myogenic differentiation 1 (MyoD) expression and growth-related gene expression in JBP muscle cells, respectively. Results: We established a muscle cell line from JBP embryos and optimized its culture conditions. These muscle cells were positive for MyoD, but not for paired box 7. The proliferation rate of these muscle cells was significantly higher in a culture medium containing bFGF and epidermal growth factor + basic fibroblast growth factor (EGF+bFGF) than that without a growth factor or containing EGF alone. Treatment with EGF and bFGF significantly induced the expression of MyoD protein, an important transcription factor in muscle cells. Moreover, we checked the changes of expression of growth-related genes in JBP muscle cells by presence or absence of growth factors. Expression level of collagen type XXI alpha 1 gene was changed only when EGF and bFGF were added together to culture media for JBP muscle cells. Conclusion: Concurrent use of EGF and bFGF increased the expression of MyoD protein, thus regulating the proliferation of JBP muscle cells and the expression of growth-related genes.

• 대한화학회지
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• 제5권1호
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• pp.33-37
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• 1961

We have shown that carboxy-peptidase destroys the biological activity of angiotensin octa-and deca-peptides. Since Proline occurs as the seventh amino acid from the amino end of the chain and since carboxypeptidase does not cleave proline from a peptid chain, it is evident that the heptapeptid H.asp-arg-val-tyr-ileu-his-pro.OH is formed by this hydrolysis. This peptide must then be biologically inactive. In order to determine whether the phenyl group of the C-terminal amino acid was the necessary requirement for biological activity of the octapeptide, $ala^8$ angiotensin octapeptide(amino acids of peptides numbered from amino end) was synthesized. For this synthesis the four dipeptides were prepared: carbobenzoxy-L-prolyl-L-alanine-P-nitrobenzyl-ester, m.p. $134-135^{\circ}C,$ carbobenzoxy-L-isoleucyl-imidazole benzyl-L-histidine methyl ester, m.p. $114-116^{\circ}C,$ carbobenzoxy-L-valyl-L-tyrosine hydrazide and carbobenzoxy B-benzyl-L-aspartyl-nitro-L-arginine. The first three dipeptides were obtained as crystalline compounds. Imidazole-benzyl-L-histidine was used in the hope that it would block the histidine imidazole against side reactions in steps subsequent to the formation of the C-terminal tetrapeptide. Also, it was through that the imidazole benzylated peptides would be easier to crystallize. This, however, was not the case. The tetrapeptide, carbobenzoxy-L-isoleucyl-L-im, benzyl-histidyl, L-prolyl-L-alanine-nitrobenzyl ester was not obtained in a crystalline form. Neither could the mono-or dihydrobromide of the tetrapeptide free base be induced to crystallize. Carbobenzoxy-L-valyl-L-tyrosine azide was condensed with the tetrapeptide free base to yield the protected hexapeptide; carbobenzoxy-L-valyl-L-tyrosyl-L-isoleucyl-L-im, benzyl, histidyl-L-Prolyl-L-alanine-nitrobenzyl ester. Upon removal of the carbobenzoxy group with hydrogen bromide in acetic acid an amorphous free base hexapeptide ester was obtained. This compound gave the correct C, H, N analysis and contained the six amino acids in the correct ratio. The octapeptide was obtained by condensing this hexapeptide with carbobenzoxy-B-benzyl-L-aspartyl-nitro, L-arginine using the mixed anhydride method of condensation. This amorphous product was proven to be homogenous by chromatography in two solvent systems and upon hydrolysis yielded the eight amino acids in correct ratio. The five protecting groups were removed from the octapeptide by hydrogenolysis over palladium black catalyst. Biological assay of the free peptide indicated that it possessed less than 0.1 per cent of both pressor and oxytocic activity of the phenylalanine8 angiotensin. This suggests that the phenyl group is a point of attachment between angiotensin and its biological receptor site.

• 한국항만경제학회지
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• 제28권3호
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• pp.235-257
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• 2012

최근 변화하는 항만수요 요구에 신속하게 대응하기 위하여 항만은 그 역할변화를 모색하고 있다. 무역을 위한 단순한 장소가 아닌 로지스틱 체인의 중요한 한 노드로서 기능을 하는 항만으로 변모하고 있다. 항만이 로지스틱 체인 속의 경쟁우위 요소로서 그 역할을 다하기 위해 항만 배후단지 공급의 필요성을 역설한 연구들이 다수 등장하였고 각 항만국들은 앞다투어 항만개발계획을 수립하고 있는 실정이다. 우리 정부 역시 "전국 무역항 항만배후단지 개발 종합계획(2006)"을 통해 아시아의 로지스틱 허브항을 육성하기 위한 항만배후단지 개발계획을 발표했다. 항만배후단지 개발을 위한 투자평가에 대한 선행연구들을 살펴보면 대부분 전통적인 투자평가 기법인 DCF(discounted cash flow)를 이용해 그 가치평가를 실시하고 있다. 그러나 항만개발사업의 경우 거대 초기 투자가 필요하고 장기간의 투자회수 기간이 소요되어 기존의 DCF법에 의한 투자평가는 미래의 불확실성에 대한 가치를 과소평가할 여지가 있다. 이에 본 연구에서는 실제 항만배후단지 경제성평가 데이터를 이용하여 기존의 DCF법과 실물옵션 가치법의 결과값을 비교 분석하였다. 그 결과 DCF에 의한 항만배후단지 경제성평가 결과는 NPV는 -73,601(백만)원, B/C ratio는 0.97, IRR은 5.06%을 보여 개발에 대한 모호함이 있으나, 실물옵션을 이용하여 추정된 확장된 NPV 값은 1,839,000(백만)원으로 개발에 대한 긍정적인 결과 값이 도출되었다. 본 연구는 실증연구를 통해 불확실성이 큰 항만투자영역에 있어 불확실성의 가치를 반영하지 못하는 DCF법을 보완할 수 있는 새로운 평가방법으로 실물옵션법의 유용성을 소개하고자 한다.

• 정보처리학회논문지B
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• 제14B권6호
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• pp.453-460
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• 2007

본 논문은 정형화되지 않은 텍스트 라인들을 추출하기 위한 방법을 보여주고 있다. 텍스트 라인들은 각기 다른 각도로 구성되고, 심하게 굴곡이 있는 모양, 그리고 텍스트 라인내의 약간의 단어 사이의 공간이 생기게 된다. 그러한 텍스트 라인들은 포스터, 주소, 그리고 예술 문서 등에서 발견된다. 제안하는 방법은 기존의 직관적인 그룹핑 방법에 기반을 두고 있지만, 하나의 라인에서 발생하는 불충분한 특징점들과 모호한 회전 등을 극복하기 위한 방법을 개발하였다. 본 논문에서 텍스트 라인들은 몇 개의 연결된 성분들로 구성되고, 이 성분들은 하나의 문자 또는 연결된 문자들의 검은색 화소들의 집합이라고 가정하였다. 제안하는 방법은 반복적으로 증가되는 임계값과 가까운 성분들은 하나의 체인으로 병합하게 되고 확장되어 길어진 체인들은 라인의 원시 체인으로서 인지된다. 그때 원시 체인들은 텍스트 라인의 부분적 회전에 따라 좌우로 확장되어 진다. 텍스트 라인의 부분적인 회전은 원시 체인이 확장될 때, 체인들의 각 면에서 재구성될 것이다. 이러한 과정을 통해서 모든 텍스트 라인들이 구성되어 진다. 제안 방법은 로고와 슬로건에서 사용된 곡면으로 쓰여진 텍스트 라인들에 대해서 실험한 결과 직선 텍스트 라인은 98%, 곡선 텍스트 라인은 94%로서 높은 추출율을 보여주고 있다.

• Asian-Australasian Journal of Animal Sciences
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• 제27권8호
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• pp.1189-1195
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• 2014

Natural resistance-associated macrophage protein 1 encoding gene (NRAMP1) plays an important role in immune response against intracellular pathogens. To evaluate the effects of NRAMP1 gene on immune capacity in pigs, tissue expression of NRAMP1 mRNA was observed by real time quantitative polymerase chain reaction (PCR), and the results revealed NRAMP1 expressed widely in nine tissues. One single nucleotide polymorphism (SNP) (ENSSSCG00000025058: g.130 C>T) in exon1 and one SNP (ENSSSCG00000025058: g.657 A>G) in intron1 region of porcine NRAMP1 gene were demonstrated by DNA sequencing and PCR-RFLP analysis. A further analysis of SNP genotypes associated with immune traits including contain of white blood cell (WBC), granulocyte, lymphocyte, monocyte (MO), rate of cytotoxin in monocyte (MC) and $CD4^-CD8^+$ T lymphocyte subpopulations in blood was carried out in four pig populations including Large White and three Chinese indigenous breeds (Wannan Black, Huai pig and Wei pig). The results showed that the SNP (ENSSSCG00000025058: g.130 C>T) was significantly associated with level of WBC % (p = 0.031), MO% (p = 0.024), MC% (p = 0.013) and $CD4^-CD8^+$ T lymphocyte (p = 0.023). The other SNP (ENSSSCG00000025058: g.657 A>G) was significantly associated with the level of MO% (p = 0.012), MC% (p = 0.019) and $CD4^-CD8^+$ T lymphocyte (p = 0.037). These results indicate that the NRAMP1 gene can be regarded as a molecular marker for genetic selection of disease susceptibility in pig breeding.

• 한국의류산업학회지
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• 제14권5호
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• pp.701-712
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• 2012

This study examines the various expressions and immanent value of fashion and beauty style based on Craig Owens's Allegory theory. I analyzed four application elements of Borrow, Site Specificity, Accumulation of Strategy, and Hybridization in? Ladygaga's Music Videos. The results are as follows. 'Borrow' presents a kitsch style and playful Pop-art style, transformation of gender from Mini Mouse body suit, telephone headpiece, and can hair. 'Site specificity' presents the temporarity of fashion material through rebirth, aging, natural extinction from the chain over the black jump suit, crime scene tape, and skull-face makeup with masculine style. 'Accumulation of strategy' presents a futuristic chic fashion style from a layered style, retrospective fashion, repetition and duplication in Music Videos. It shows the physical beauty of an Asian warrior style in Poker Face. 'Hybridization' present Cyborg feminism and 'Will of Power' from iPOD LCD glasses and Pyro-Bra. The Pyro-Bra represents how the female body can be used as a weapon in the outfits of Lady Gaga. In addition, Immanent value is as follows. Textual interaction with high art is accomplished through a combination of contemporary social and cultural significance to understand the cultural code and to extend the value. Combined with high culture, popular music genre is accomplished through musical appreciation by a woman in fashion styling and sensual pleasures of the body as a tool to express a sublime advantage. Fashion style is accomplished by overcoming a self-transcendent body image representation. The way in which mutual coupling dismantling, destruction, and uncertainty is to re-launch the static, with a pluralistic context of Textuality.

• 한국유기농업학회지
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• 제28권3호
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• pp.367-386
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• 2020

The significance of this study was to analyze the quality value of organic livestock pork for the first time based on the results of managing and testing the cycling organic farming of black pork and vegetables within farm for two years. The results of analysis could be summarized as follows. First, the pork of experimental group with crop-livestock cycling farming showed the excellent quality and high consumer preference compared to the control group of general pork or pork from non-crop-livestock cycling organic farming. In the content ratio of Omega-3 as a representative essential fatty acid, it was 1.46 that was about 2.8 times more than general pork (0.52). In case of Omega-6, it had about 2.5 times more than general pork. Especially, the U/S ratio value which was the content ratio of Unsaturated Fatty Acid (UFA, U) of Saturated Fatty Acid (SFA, S), was largely shown in pork (2.93) from cycling organic farming. Second, it would be necessary to maintain the economies of scope shown in crop-livestock cycling organic farming, and the high quality value of livestock products. For this, there should be a value chain model that could realize the economies of scope and economies of scale at the same time based on scaling and diversification through cooperative organization between farmers. Through this, it would be possible to establish a cycling model called 'community cooperative agriculture' by forming local internal markets through cooperation of production-processing and integration of distribution-sale-consumption. For the managerial activation of this cooperative organization, the government should promote/support the small crop-livestock cycling organic farming cooperative organization in local unit. For securing the reliability of crop-livestock cycling organic agricultural products and crop-livestock cycling organic livestock products, it would be necessary to review the introduction of Participatory Guarantee System (PGS).

• 한국의상디자인학회지
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• 제16권3호
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• pp.219-238
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• 2014

This study attempted to investigate present condition and product planning of global eco-fashion brands that harmonize fashion and sustainability. As research subjects, this study selected 97 oversea fashion brands mentioned in books related to eco-fashion, Black(2011), Brown(2010), Fuad-luke(2009). As for research methods, materials and ethical practices of these selected 97 brands through literature data and their internet site homepages. This study analyzed oversea eco-brands collected 26 British brands, 22 American brands, 36 European brands such as Germany, France, Italy, Sweden, Spain, Finland and so on, except Britain and 13 other regions including Japan, India, Canada, Mexico, and New zealand. In conclusion, the product planning characteristics of these oversea eco-fashion brands can be summarized as follows; community and fair trade, ecological and slow design, recycle, reuse, redesign, and new eco-models. Firstly, brands of 'community and fair trade' manufactured products through fair trade and local community's artisan by ethical practices with organic fabrics. Secondly, brands of 'ecological and slow design' pursued timeless design and multi-functional design as luxury eco-fashion styles. They used organic textiles, hemp, bamboo, soya, tencell, sea cell, and self-sustaining plants. Thirdly, brands of 'recycle, reuse, redesign' aimed for upcycling high-end fashion and used vintage clothes, textile scraps, PET, parachutes, tires, safety belts, advertising banner and so on. In addition, brands of 'new models as eco-fashion' suggested zero-waste cutting, recycling over-printing technology, new sustainable business model, and ethical practices in the supply chain of the fashion industry.