Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Effects of c.494A>C and c.267T>G SNPs in OCX-32 Gene of Korean Native Chicken on Egg Production Traits

OCX-32 유전자 내 c.494A>C 및 c.267T>G SNP이 한국 재래닭 산란형질에 미치는 효과 분석

  • Lee, Ji-Yeon (College of Animal Life Sciences, Kangwon National University) ;
  • Choi, So-Young (College of Animal Life Sciences, Kangwon National University) ;
  • Kim, Chong-Dae (National Institute of Animal Science) ;
  • Hong, Yeong Ho (Department of Animal Science and Technology, Chung-Ang University) ;
  • Jeong, Dong Kee (Department of Animal Biotechnology, Jeju National University) ;
  • Lee, Sung-Jin (College of Animal Life Sciences, Kangwon National University)
  • 이지연 (강원대학교 동물생명과학대학) ;
  • 최소영 (강원대학교 동물생명과학대학) ;
  • 김종대 (농촌진흥청 국립축산과학원) ;
  • 홍영호 (중앙대학교 동물생명공학과) ;
  • 정동기 (제주대학교 생명공학부) ;
  • 이성진 (강원대학교 동물생명과학대학)
  • Received : 2014.07.21
  • Accepted : 2014.09.01
  • Published : 2014.09.30
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Abstract

The identification and utilization of potential candidate genes with significant effects on economically important traits have become increasingly important in poultry breeding programs. The ovocalyxin-32 (OCX-32) gene is located chromosome 9 in chicken, plays an important role in eggshell formation. This study was performed to assess the association between single nucleotide polymorphisms (SNPs) of OCX-32 gene and egg production traits in the Korean native chicken. Four Korean native chicken population (n = 181; including 46 females of Ogol, 46 females of white, 43 females of gray and 46 females of black) were used to analyze two SNPs (c.494A>C and c.267T>G) in the OCX-32 gene by PCR-RFLP (Polymerase Chain Reaction-Restricted Fragment Length Polymorphism). We measured egg production traits of age at first egg, first egg weight, egg production ratio and egg weight. The SNP c.494A>C was significantly associated with egg production ratio in Korean Ogol chickens (p<0.001) and egg weight in Korean white chickens (p<0.05). SNP c.267T>C was significantly associated with egg weight in Korean Ogol chickens (p<0.05). But there was no significant association in Korean gray and black chickens. Results suggest the possibility of using molecular markers in OCX-32 gene as a tool for performance and egg production traits in Korean native chicken breeding program.

가금 사육 프로그램에서 경제적으로 중요한 형질에 잠재적인 후보 유전자의 식별 및 활용은 점점 더 중요해지고 있다. Ovocalyxin-32(OCX-32) 유전자는 닭의 9번 염색체에 위치하며, 난각을 형성하는데 중요한 역할을 한다. 본 연구의 목적은 한국 재래닭의 OCX-32 유전자 내 SNP의 유전자형 결정과 산란 형질과의 연관성을 분석하기 위해 수행하였다. PCR-RFLP 방법을 통해 한국 오골계 46수, 백색 46수, 회색 43수, 흑색 46수를 포함한 총 181수의 한국 재래닭 암컷 4종의 SNP을 분석하였다. 산란 형질은 시산일령, 시산난중, 산란율, 난중의 4가지 항목을 포함하여 측정하였다. OCX-32 유전자 내 c.494A>C SNP은 오골계의 산란율과 유의적인 차이를 나타냈으며(p<0.001), 백색 재래닭에서는 난중과 유의적인 상관관계가 있었다(p<0.05). c.267T>G SNP은 오골계의 난중과 유의적인 연관성이 나타났다(p<0.05). 하지만 회색과 흑색 재래닭에서는 유의적인 상관관계가 나타나지 않았다. 본 연구 결과, 한국 재래닭의 사육 프로그램에서 산란형질을 선발하는 마커로 사용되기까지 추후 더 많은 개체군을 통한 연구가 요구되나, OCX-32 유전자 내 c.494A>C와 c.267T>G의 단일염기변이가 한국 재래닭 중 오골계와 백색 재래닭에서 산란 특성에 따른 DNA 선발 마커로서 활용할 수 있을 것으로 사료된다.

Keywords

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