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http://dx.doi.org/10.5536/KJPS.2014.41.3.191

Effects of c.494A>C and c.267T>G SNPs in OCX-32 Gene of Korean Native Chicken on Egg Production Traits  

Lee, Ji-Yeon (College of Animal Life Sciences, Kangwon National University)
Choi, So-Young (College of Animal Life Sciences, Kangwon National University)
Kim, Chong-Dae (National Institute of Animal Science)
Hong, Yeong Ho (Department of Animal Science and Technology, Chung-Ang University)
Jeong, Dong Kee (Department of Animal Biotechnology, Jeju National University)
Lee, Sung-Jin (College of Animal Life Sciences, Kangwon National University)
Publication Information
Korean Journal of Poultry Science / v.41, no.3, 2014 , pp. 191-196 More about this Journal
Abstract
The identification and utilization of potential candidate genes with significant effects on economically important traits have become increasingly important in poultry breeding programs. The ovocalyxin-32 (OCX-32) gene is located chromosome 9 in chicken, plays an important role in eggshell formation. This study was performed to assess the association between single nucleotide polymorphisms (SNPs) of OCX-32 gene and egg production traits in the Korean native chicken. Four Korean native chicken population (n = 181; including 46 females of Ogol, 46 females of white, 43 females of gray and 46 females of black) were used to analyze two SNPs (c.494A>C and c.267T>G) in the OCX-32 gene by PCR-RFLP (Polymerase Chain Reaction-Restricted Fragment Length Polymorphism). We measured egg production traits of age at first egg, first egg weight, egg production ratio and egg weight. The SNP c.494A>C was significantly associated with egg production ratio in Korean Ogol chickens (p<0.001) and egg weight in Korean white chickens (p<0.05). SNP c.267T>C was significantly associated with egg weight in Korean Ogol chickens (p<0.05). But there was no significant association in Korean gray and black chickens. Results suggest the possibility of using molecular markers in OCX-32 gene as a tool for performance and egg production traits in Korean native chicken breeding program.
Keywords
OCX-32; SNP; Korean native chicken; egg production; PCR-RFLP;
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