• Journal of Life Science
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• v.20 no.8
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• pp.1249-1253
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• 2010

Sericin is a type of high molecular weight water-soluble glycoprotein surrounding fibroin (silk protein) that has been used as a cell culture supplement and accelerates cell proliferation in various serum-free media. The purpose of this study was to investigate the enhancing effect of thyroglobulin (Tg) secretion by sericin in thyrocytes, FRTL-5 cells. While Tg-mRNA expression was not enhanced, a secreted form of Tg was obviously increased by sericin. In this status, expression of both endoplasmic reticulum (ER) molecular chaperones (Bip & calreticulin) and ER membrane proteins (IRE1, PERK & ATF6) was enhanced. The proximal step of IRE1, XBP1 mRNA splicing was slightly detected however, the proximal step of PERK, phosphorylation of $eIF2{\alpha}$, was changeless. In addition, sericin enhanced cell viability by the MTT assay. The above results showing the ability of sericin to promote protein production demonstrated its potential usefulness as a new biomaterial.

• The Journal of Engineering Geology
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• v.9 no.3
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• pp.187-206
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• 1999

A computer program to numerically predict the permeability tensor of fractured rocks is developed using information on discontinuities which Borehole Televiewer and Borehole Image Processing System (BIPS) provide. It uses orientation and thickness of a large number of discontinuities as input data, and calculates relative values of the 9 elements consisting of the permeability tensor by the formulation based on the EPM model, which regards a fractured rock as a homogeneous, anisotropic porous medium. In order to assess feasibility of the program on field sites, the numerically calculated tensor was obtained using BIPS logs and compared to the results of pumping test conducted in the boreholes of the study area. The degree of horizontal anisotropy and the direction of maximum horizontal permeability are 2.8 and $N77^{\circ}CE$, respectively, determined from the pumping test data, while 3.0 and $N63^{\circ}CE$ from the numerical analysis by the developed program. Disagreement between two analyses, especially for the principal direction of anisotropy, seems to be caused by problems in analyzing the pumping test data, in applicability of the EPM model and the cubic law, and in simplified relationship between the crack size and aperture. Aside from these problems, consideration of hydraulic parameters characterizing roughness of cracks and infilling materials seems to be required to improve feasibility of the proposed program. Three-dimensional assessment of its feasibility on field sites can be accomplished by conducting a series of cross-hole packer tests consisting of an injecting well and a monitoring well at close distance.

• The Korean Journal of Physiology and Pharmacology
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• v.11 no.6
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• pp.239-246
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• 2007

Expressions of endoplasmic reticulum stress response (ERSR) genes were examined during the neuronal differentiation of rat fetal cortical precursor cells (rCPC) and rat pheochromocytoma PC12 cells. When rCPC were differentiated into neuronal cells for 7 days, early stem cell marker, nest in, expression was decreased from day 4, and neuronal markers such as neurofilament-L, -M and Tuj1 were increased after day 4. In this condition, expressions of BIP, ATF6, and phosphorylated PERK as well as their down stream signaling molecules such as CHOP, ATF4, XBP1, GADD34, Nrf2 and $p58^{IPK}$ were significantly increased, suggesting the induction of ERSR during neuronal differentiation of rCPC. ERSR was also induced during the differentiation of PC12 cells for 9 days with NGF. Neurofilament-L transcript was time-dependently increased. Both mRNA and protein levels of Tuj1 were increased after the induction, and the significant increase in NeuN was observed at day 9. Similar to the expression patterns of neuronal markers, BIP/GRP78 and CHOP mRNAs were highly increased at day 9, and ATF4 mRNA was also increased from day 7. These results strongly suggest the induction and possible role of ERSR in neuronal differentiation process. Further study to identify targets responsible for neuronal induction will be necessary.

• Journal of Korean Port Research
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• v.13 no.1
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• pp.51-64
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• 1999

This study is on the focus of the cost-effective and time-effective in the BCTOC which commenced the On Dock Termainal System in 1998 for the first time in Korea. On this process of study two categories should be considered : (1) Improvement on Business Process (2) Improvement on Information Technology. With these two accesses a new future-oriented and prospective model could be put on our hand as follows. (1) Improvement on Business Process \circled1Consistency of container In-Out Operation \circled2Simplicity of Rate appraisal \circled3Unified operation on Billing and Request (2) Improvement on Information Technology \circled1Scale up the Information Application \circled2Constructing an one-spot simultaneous Information Holding System \circled3Combination of Internet and Integrated Database System.

• Journal of Power System Engineering
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• v.21 no.1
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• pp.70-79
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• 2017

The output power of turbine is greatly affected by the losses generated within the passage. In order to develop a better turbine or loss models, an experimental study was conducted using a linear cascade experimental apparatus. The total pressure loss and flow structures were measured at two cross-sectional planes located downstream of blade row. Measurement was conducted in a steady state for the several different locations of the blade row along the rotational direction. The blade row moved by 20 % of the pitch, and tip clearance was varied from 2% to 8%. Axial-type blades were used and its blade chord was 200mm. A square nozzle was applied and its size was $200mm{\times}200mm$. The experiment was conducted at a Reynolds number of $3{\times}10^5$ based on the chord. Nozzle flow angle sets to $65^{\circ}$ based on the axial direction and the solidity of blade row was 1.38. From the experimental results, the total pressure loss was greatly varied in the receding region than in the entering region. The flow properties within the blade passage were strongly changed according to the location of blade row.

• BMB Reports
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• v.45 no.8
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• pp.482-487
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• 2012

To identify the novel inhibitors of endoplasmic reticulum stress-induced cell death, we performed a high throughput assay with a chemical library containing a total of 3,280 bioactive small molecules. Cyclosporine A and bromocriptine were identified as potent inhibitors of thapsigargiin-induced cell death (cut-off at $4{\sigma}$ standard score). However, U74389G, the potent inhibitor of lipid peroxidation had lower activity in inhibiting cell death. The inhibition effect of cyclosporine A and bromocriptine was specific for only thapsigargin-induced cell death. The mechanism of inhibition by these compounds was identified as modification of the expression of glucose regulated protein-78 (GRP-78/Bip) and inhibition of phosphorylation of p38 mitogen activated protein kinase (MAPK). However, these compounds did not inhibit the same events triggered by tunicamycin, which was in agreement with the cell survival data. We suggest that the induction of protective unfolded protein response by these compounds confers resistance to cell death. In summary, we identified compounds that may provide insights on cell death mechanisms stimulated by ER stress.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.9 no.3
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• pp.39-50
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• 2006

In this study, the water quality investigated during 2002s to 2004s for Anyang river and its affluent that was based on biological and chemical methods as well as ecological index of each streams. The improving of water quality and the multiplicity of specious in the most streams except for Anyang, which was due to the improving of water quality and water resource. The seasonal dry states are completely disappeared, but water quality of Anyang main stream was classified as fifth grades water with BIP 8.51~10.00 and BOD 8.16~14.4mg/l in Anyang stream overall. And alpha-mesosaprobic in upper, mid parts and polysaprobic in down parts of Anyang main stream are appeared according to Saprobien system, respectively. The water supply of treated sewage is appeared that upgrading effects of water quality in Anyang main stream and affluents of Anyang city area, but the practical effects of "Saving Anyang River" could not gained because the water quality of other branches in upstream parts than upstream measuring point of Anyang main stream, Wanggok stream Sanbon stream in Gunpo and Eeiwang cities, was not improved.

• Proceedings of the KSEG Conference
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• 2005.04a
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• pp.157-164
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• 2005

BIPS와 Televiewer를 이용하여 10개의 지하수 관측공에서 획득된 1,591개 불연속면을 분석하여 지하수 유동과의 관계를 비교${\cdot}$분석하였다. 연구지역 내의 지형은 산계가 잘 발달된 계곡형 지형이며, 수계의 발달은 매우 미약하다. 연구지역 주변에는 북북동-남남서 방향의 양산단층과 동래단층이 발달되어 있으며, 연구지역 내에는 EW방향의 선구조도 발달되어 있다. 지하수공내에서 조사된 불연속면의 주 방향은 위성사진에서 판독된 선구조의 방향과 대부분 일치한다. 지구통계기법을 이용하여 작성된 지하수위 등고선도에서 추정된 지하수 유동방향과 유향${\cdot}$유속기에서 측정된 지하수 유향은 거의 일치하지만, 단열암반에 발달된 불연속면의 방향과 지하수 유동방향은 2가지로 해석되었다. 첫째는 단열암반에 발달된 불연속면의 방향과 지하수 유동방향이 잘 일치하는 경우(BH-01, 03, 04, 12공), 둘째는 불연속면의 방향과 지하수 유동방향이 잘 일치하지 않는 경우(BH-02, 05, 07, 11) 이다. 지하수 유동방향이 불연속면의 방향과 일치하지 않는 경우는 지형의 변화에 더 영향을 받는 것으로 나타났다. 따라서 단열암반 내에서 지하수의 유동은 단열의 방향성에 영향을 받지만, 지형의 특성에도 큰 영향을 받는다.

• Clinical and Experimental Reproductive Medicine
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• v.42 no.1
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• pp.1-7
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• 2015

Stress coping mechanisms are critical to minimize or overcome damage caused by ever changing environmental conditions. They are designed to promote cell survival. The unfolded protein response (UPR) pathway is mobilized in response to the accumulation of unfolded proteins, ultimately in order to regain endoplasmic reticulum (ER) homeostasis. Various elements of coping responses to ER stress including Perk, Ask1, Bip, Chop, Gadd34, Ire1, Atf4, Atf6, and Xbp1 have been identified and were found to be inducible in oocytes and preimplantation embryos, suggesting that, as a normal part of the cellular adaptive mechanism, these coping responses, including the UPR, play a pivotal role in the development of preimplantation embryos. As such, the UPR-associated molecules and pathways may become useful markers for the potential diagnosis of stress conditions for preimplantation embryos. After implantation, ER stress-induced coping responses become physiologically important for a normal decidual response, placentation, and early organogenesis. Attenuation of ER stress coping responses by tauroursodeoxycholate and salubrinal was effective for prevention of cell death of cultured embryos. Further elucidation of new and relevant ER stress coping responses in periimplantation embryos might contribute to a comprehensive understanding of the regulation of normal development of embryonic development and potentiation of embryonic development in vitro.

• The Korean Journal of Zoology
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• v.35 no.4
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• pp.456-465
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• 1992

The 94 KDa glucose-resulsted Protein (SH 94), one of stress Proteins, is a Ca2+-binding protein in the endoplasmic reticulum (ER). In this study, the possible effect of Ca2+ on the native conformation of grp 94 was examined. When the purified grp 94 was analyzed by Sel filtration in the presence of either EGTA or CaCl2, it was eluted with apparent molecular weight (MW) of 100 KDa in both cases. When similarly analyzed with microtome or cell Ivsate, however, srp 94 was eluted with apparent IW of 200 KDa in the presence of E6TA, while with apparent MW of 100 KDa in the presence of CaCl2, indicating possible association of grp 94 with one or more other proteins in the absence of CaCl2. Consequently, immunoprecipitation with anti-grp 94 was carried out to determine which proteins specifically interact with grp 94. It is sho%un that srp 94 may interact, in a Ca2+_dependent manner. with other proteins including BiP (grp 78) which is also a stress protein in the ER.